PMID- 27078494
OWN - NLM
STAT- MEDLINE
DCOM- 20160901
LR  - 20170220
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 4
DP  - 2016
TI  - Frequency, Antimicrobial Resistance and Genetic Diversity of Klebsiella
      pneumoniae in Food Samples.
PG  - e0153561
LID - 10.1371/journal.pone.0153561 [doi]
AB  - This study aimed to assess the frequency of Klebsiella pneumoniae in food samples
      and to detect antibiotic resistance phenotypes, antimicrobial resistance genes
      and the molecular subtypes of the recovered isolates. A total of 998 food samples
      were collected, and 99 (9.9%) K. pneumoniae strains were isolated; the
      frequencies were 8.2% (4/49) in fresh raw seafood, 13.8% (26/188) in fresh raw
      chicken, 11.4% (34/297) in frozen raw food and 7.5% (35/464) in cooked food
      samples. Antimicrobial resistance was observed against 16 antimicrobials. The
      highest resistance rate was observed for ampicillin (92.3%), followed by
      tetracycline (31.3%), trimethoprim-sulfamethoxazole (18.2%), and chloramphenicol 
      (10.1%). Two K. pneumoniae strains were identified as extended-spectrum
      beta-lactamase (ESBL)-one strain had three beta-lactamases genes (blaSHV,
      blaCTX-M-1, and blaCTX-M-10) and one had only the blaSHV gene. Nineteen
      multidrug-resistant (MDR) strains were detected; the percentage of MDR strains in
      fresh raw chicken samples was significantly higher than in other sample types
      (P<0.05). Six of the 18 trimethoprim-sulfamethoxazole-resistant strains carried
      the folate pathway inhibitor gene (dhfr). Four isolates were screened by PCR for 
      quinolone resistance genes; aac(6')-Ib-cr, qnrB, qnrA and qnrS were detected. In 
      addition, gyrA gene mutations such as T247A (Ser83Ile), C248T (Ser83Phe), and
      A260C (Asp87Ala) and a parC C240T (Ser80Ile) mutation were identified. Five
      isolates were screened for aminoglycosides resistance genes; aacA4, aacC2, and
      aadA1 were detected. Pulsed-field gel electrophoresis-based subtyping identified 
      91 different patterns. Our results indicate that food, especially fresh raw
      chicken, is a reservoir of antimicrobial-resistant K. pneumoniae, and the
      potential health risks posed by such strains should not be underestimated. Our
      results demonstrated high prevalence, antibiotic resistance rate and genetic
      diversity of K. pneumoniae in food in China. Improved control and prevention
      strategies are urgently needed.
FAU - Guo, Yumei
AU  - Guo Y
AD  - Shijiazhuang Center for Disease Control and Prevention, Shijiazhuang, People's
      Republic of China.
FAU - Zhou, Haijian
AU  - Zhou H
AD  - State Key Laboratory for Infectious Disease Prevention and Control, National
      Institute for Communicable Disease Control and Prevention, Chinese Center for
      Disease Control and Prevention, Beijing, People's Republic of China.
AD  - Collaborative Innovation Center for Diagnosis and Treatment of Infectious
      Diseases, Hangzhou, People's Republic of China.
FAU - Qin, Liyun
AU  - Qin L
AD  - Shijiazhuang Center for Disease Control and Prevention, Shijiazhuang, People's
      Republic of China.
FAU - Pang, Zhizhao
AU  - Pang Z
AD  - Shijiazhuang Center for Disease Control and Prevention, Shijiazhuang, People's
      Republic of China.
FAU - Qin, Tian
AU  - Qin T
AD  - State Key Laboratory for Infectious Disease Prevention and Control, National
      Institute for Communicable Disease Control and Prevention, Chinese Center for
      Disease Control and Prevention, Beijing, People's Republic of China.
AD  - Collaborative Innovation Center for Diagnosis and Treatment of Infectious
      Diseases, Hangzhou, People's Republic of China.
FAU - Ren, Hongyu
AU  - Ren H
AD  - State Key Laboratory for Infectious Disease Prevention and Control, National
      Institute for Communicable Disease Control and Prevention, Chinese Center for
      Disease Control and Prevention, Beijing, People's Republic of China.
FAU - Pan, Zhuo
AU  - Pan Z
AD  - Shijiazhuang Center for Disease Control and Prevention, Shijiazhuang, People's
      Republic of China.
FAU - Zhou, Jikun
AU  - Zhou J
AD  - Shijiazhuang Center for Disease Control and Prevention, Shijiazhuang, People's
      Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160414
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antineoplastic Agents)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/pharmacology
MH  - Chickens
MH  - *Drug Resistance, Bacterial
MH  - *Food Microbiology
MH  - *Genes, Bacterial
MH  - *Genetic Variation
MH  - Humans
MH  - Klebsiella Infections/drug therapy/*microbiology
MH  - Klebsiella pneumoniae/drug effects/*genetics/*isolation & purification
MH  - Mutation
MH  - Poultry/microbiology
MH  - Seafood/microbiology
PMC - PMC4831839
EDAT- 2016/04/15 06:00
MHDA- 2016/09/02 06:00
CRDT- 2016/04/15 06:00
PHST- 2015/12/16 00:00 [received]
PHST- 2016/03/31 00:00 [accepted]
PHST- 2016/04/15 06:00 [entrez]
PHST- 2016/04/15 06:00 [pubmed]
PHST- 2016/09/02 06:00 [medline]
AID - 10.1371/journal.pone.0153561 [doi]
AID - PONE-D-15-50034 [pii]
PST - epublish
SO  - PLoS One. 2016 Apr 14;11(4):e0153561. doi: 10.1371/journal.pone.0153561.
      eCollection 2016.