PMID- 27043016
OWN - NLM
STAT- MEDLINE
DCOM- 20160812
LR  - 20170220
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 4
DP  - 2016
TI  - Estimating the Burden of Leptospirosis among Febrile Subjects Aged below 20 Years
      in Kampong Cham Communities, Cambodia, 2007-2009.
PG  - e0151555
LID - 10.1371/journal.pone.0151555 [doi]
AB  - BACKGROUND: Leptospirosis is an emerging but neglected public health challenge in
      the Asia/Pacific Region with an annual incidence estimated at 10-100 per 100,000 
      population. No accurate data, however, are available for at-risk rural Cambodian 
      communities. METHOD: We conducted anonymous, unlinked testing for IgM antibodies 
      to Leptospira spp. on paired sera of Cambodian patients <20 years of age between 
      2007-2009 collected through active, community-based surveillance for febrile
      illnesses in a convenience sample of 27 rural and semi-rural villages in four
      districts of Kampong Cham province, Cambodia. Leptospirosis testing was done on
      paired serological samples negative for Dengue, Japanese encephalitis and
      Chikungunya viruses after random selection. Convalescent samples found positive
      while initial samples were negative were considered as proof of acute infection. 
      We then applied a mathematical model to estimate the risk of fever caused by
      leptospirosis, dengue or other causes in rural Cambodia. RESULTS: A total of 630 
      samples are coming from a randomly selected subset of 2358 samples. IgM positive 
      were found on the convalescent serum sample, among which 100 (15.8%) samples were
      IgM negative on an earlier sample. Seventeen of these 100 seroconversions were
      confirmed using a Microagglutination Test. We estimated the probability of having
      a fever due to leptospirosis at 1. 03% (95% Credible Interval CI: 0. 95%-1. 22%) 
      per semester. In comparison, this probability was 2. 61% (95% CI: 2. 55%, 2. 83%)
      for dengue and 17. 65% (95% CI: 17. 49%, 18. 08%) for other causes. CONCLUSION:
      Our data from febrile cases aged below 20 years suggest that the burden of
      leptospirosis is high in rural Cambodian communities. This is especially true
      during the rainy season, even in the absence of identified epidemics.
FAU - Hem, Sopheak
AU  - Hem S
AD  - Institut Pasteur in Cambodia, Phnom Penh, Cambodia.
FAU - Ly, Sowath
AU  - Ly S
AD  - Institut Pasteur in Cambodia, Phnom Penh, Cambodia.
FAU - Votsi, Irene
AU  - Votsi I
AD  - Mathematical Modelling of Infectious Diseases Unit, Institut Pasteur, Paris,
      France.
FAU - Vogt, Florian
AU  - Vogt F
AD  - Masters of Science in Epidemiology, London School of Hygiene and Tropical
      Medicine, London, United Kingdom.
FAU - Asgari, Nima
AU  - Asgari N
AD  - World Health Organization, Phnom Penh, Cambodia.
FAU - Buchy, Philippe
AU  - Buchy P
AD  - Institut Pasteur in Cambodia, Phnom Penh, Cambodia.
FAU - Heng, Seiha
AU  - Heng S
AD  - Institut Pasteur in Cambodia, Phnom Penh, Cambodia.
FAU - Picardeau, Mathieu
AU  - Picardeau M
AD  - Institut Pasteur, National Reference Center and WHO Collaborating Center for
      Leptospirosis, Paris, France.
FAU - Sok, Touch
AU  - Sok T
AD  - Communicable Diseases Control Department of the Ministry of Health, Phnom Penh,
      Cambodia.
FAU - Ly, Sovann
AU  - Ly S
AD  - Communicable Diseases Control Department of the Ministry of Health, Phnom Penh,
      Cambodia.
FAU - Huy, Rekol
AU  - Huy R
AD  - National Dengue Control Program, National Center for Entomology, Parasitology,
      and Malaria Control, Phnom Penh, Cambodia.
FAU - Guillard, Bertrand
AU  - Guillard B
AD  - Institut Pasteur in Cambodia, Phnom Penh, Cambodia.
FAU - Cauchemez, Simon
AU  - Cauchemez S
AD  - Mathematical Modelling of Infectious Diseases Unit, Institut Pasteur, Paris,
      France.
FAU - Tarantola, Arnaud
AU  - Tarantola A
AD  - Institut Pasteur in Cambodia, Phnom Penh, Cambodia.
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20160404
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Immunoglobulin M)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antibodies, Bacterial/blood
MH  - Cambodia/epidemiology
MH  - Child
MH  - Child, Preschool
MH  - Dengue/blood/epidemiology
MH  - Female
MH  - Humans
MH  - Immunoglobulin M/blood
MH  - Infant
MH  - Infant, Newborn
MH  - Leptospirosis/blood/*epidemiology
MH  - Male
MH  - *Rural Population
MH  - Social Planning
PMC - PMC4820258
EDAT- 2016/04/05 06:00
MHDA- 2016/08/16 06:00
CRDT- 2016/04/05 06:00
PHST- 2013/04/18 00:00 [received]
PHST- 2016/03/01 00:00 [accepted]
PHST- 2016/04/05 06:00 [entrez]
PHST- 2016/04/05 06:00 [pubmed]
PHST- 2016/08/16 06:00 [medline]
AID - 10.1371/journal.pone.0151555 [doi]
AID - PONE-D-13-15867 [pii]
PST - epublish
SO  - PLoS One. 2016 Apr 4;11(4):e0151555. doi: 10.1371/journal.pone.0151555.
      eCollection 2016.