PMID- 26823121
OWN - NLM
STAT- MEDLINE
DCOM- 20170630
LR  - 20180217
IS  - 1464-410X (Electronic)
IS  - 1464-4096 (Linking)
VI  - 117
IP  - 6
DP  - 2016 Jun
TI  - Testosterone modulates endothelial progenitor cells in rat corpus cavernosum.
PG  - 976-81
LID - 10.1111/bju.13438 [doi]
AB  - OBJECTIVE: To investigate the effects of testosterone on cavernosal endothelial
      progenitor cells (EPCs) in a castrated rat model. MATERIALS AND METHODS: In all, 
      45 male Sprague-Dawley rats (12-weeks old) were divided into control, surgical
      castration, and castration with testosterone replacement groups. The rats were
      castrated under ketamine anaesthesia, and testosterone was administered by daily 
      subcutaneous injection of 3 mg/kg testosterone propionate. The corpus cavernosum 
      was obtained after perfusion with 10 mL saline via the abdominal aorta 4 weeks
      later. The expression of EPC-specific markers [cluster of differentiation 34
      (CD34), fetal liver kinase 1 (Flk1), and vascular endothelial (VE)-cadherin] was 
      evaluated by flow cytometry analysis and immunofluorescence staining. RESULTS:
      CD34+/Flk1+ and CD34+/VE-cadherin+ cells were detected in the cavernosal
      sinusoidal endothelial space. Flow cytometry analysis showed that CD34 and Flk1
      double positive cells (EPCs) comprised approximately 3.79% of the corpus
      cavernosum in normal rats. The percentage of EPC marker-positive cells decreased 
      significantly in the castration group (2.8%; P < 0.05) and was restored to 3.56% 
      after testosterone supplementation. Confocal microscopy revealed that the numbers
      of CD34+/Flk1+ and CD34+/VE-cadherin+ cells decreased in castrated rats compared 
      with controls, but were similar to control levels in rats receiving testosterone 
      replacement. CONCLUSIONS: The EPC markers were expressed in the cavernosal
      sinusoidal endothelial space, and the numbers of resident EPCs were regulated by 
      testosterone. These results suggest that testosterone replacement therapy may
      improve erectile function by modulating EPCs in patients with hypogonadism.
CI  - (c) 2016 The Authors BJU International (c) 2016 BJU International Published by
      John Wiley & Sons Ltd.
FAU - Hwang, Insang
AU  - Hwang I
AD  - Department of Urology, Chonnam National University Medical School, Sexual
      Medicine Research Center, Chonnam National University, Gwangju, Korea.
FAU - Lee, Hyun-Suk
AU  - Lee HS
AD  - Department of Urology, Chonnam National University Medical School, Sexual
      Medicine Research Center, Chonnam National University, Gwangju, Korea.
FAU - Yu, Ho Song
AU  - Yu HS
AD  - Department of Urology, Chonnam National University Medical School, Sexual
      Medicine Research Center, Chonnam National University, Gwangju, Korea.
FAU - Kim, Mi Eun
AU  - Kim ME
AD  - Department of Biology, BK21-plus Research Team for Bioactive Control Technology, 
      College of Natural Sciences, Chosun University, Gwangju, Korea.
FAU - Lee, Jun Sik
AU  - Lee JS
AD  - Department of Biology, BK21-plus Research Team for Bioactive Control Technology, 
      College of Natural Sciences, Chosun University, Gwangju, Korea.
FAU - Park, Kwangsung
AU  - Park K
AD  - Department of Urology, Chonnam National University Medical School, Sexual
      Medicine Research Center, Chonnam National University, Gwangju, Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160225
PL  - England
TA  - BJU Int
JT  - BJU international
JID - 100886721
RN  - 3XMK78S47O (Testosterone)
RN  - EC 2.7.10.1 (Vascular Endothelial Growth Factor Receptor-2)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Endothelial Progenitor Cells/*cytology/*drug effects/pathology
MH  - Erectile Dysfunction/*drug therapy/metabolism/pathology
MH  - Flow Cytometry
MH  - *Hormone Replacement Therapy
MH  - Male
MH  - Orchiectomy
MH  - Penis/*cytology/*drug effects/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Testosterone/administration & dosage/*pharmacology/therapeutic use
MH  - Vascular Endothelial Growth Factor Receptor-2/metabolism
OTO - NOTNLM
OT  - *castration
OT  - *endothelial progenitor cells
OT  - *penis
OT  - *rats
OT  - *testosterone
EDAT- 2016/01/30 06:00
MHDA- 2017/07/01 06:00
CRDT- 2016/01/30 06:00
PHST- 2016/01/30 06:00 [entrez]
PHST- 2016/01/30 06:00 [pubmed]
PHST- 2017/07/01 06:00 [medline]
AID - 10.1111/bju.13438 [doi]
PST - ppublish
SO  - BJU Int. 2016 Jun;117(6):976-81. doi: 10.1111/bju.13438. Epub 2016 Feb 25.