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Cancer Immunol Res. 2019 May;7(5):784-796. doi: 10.1158/2326-6066.CIR-18-0517. Epub 2019 Mar 14.

A Transcriptionally Distinct CXCL13+CD103+CD8+ T-cell Population Is Associated with B-cell Recruitment and Neoantigen Load in Human Cancer.

Author information

1
Department of Obstetrics and Gynecology, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands.
2
Institute of Cancer and Genomic Sciences, University of Birmingham, Birmingham, United Kingdom.
3
Department of Genetics, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands.
4
Department of Pathology, Leiden University Medical Center, Leiden University, Leiden, the Netherlands.
5
Trev and Joyce Deeley Research Centre, BC Cancer, Victoria, British Columbia, Canada.
6
Department of Radiation Oncology, Leiden University Medical Center, Leiden University, Leiden, the Netherlands.
7
Molecular and Population Genetics Laboratory, The Wellcome Trust Centre for Human Genetics and Oxford Cancer Centre, University of Oxford, Oxford, United Kingdom.
8
NIHR Oxford Biomedical Research Centre, Oxford University Hospitals NHS Foundation Trust and John Radcliffe Hospital, Oxford, United Kingdom.
9
Department of Obstetrics and Gynecology, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands. m.de.bruyn@umcg.nl.

Abstract

The chemokine CXCL13 mediates recruitment of B cells to tumors and is essential for the formation of tertiary lymphoid structures (TLSs). TLSs are thought to support antitumor immunity and are associated with improved prognosis. However, it remains unknown whether TLSs are formed in response to the general inflammatory character of the tumor microenvironment, or rather, are induced by (neo)antigen-specific adaptive immunity. We here report on the finding that the TGFβ-dependent CD103+CD8+ tumor-infiltrating T-cell (TIL) subpopulation expressed and produced CXCL13. Accordingly, CD8+ T cells from peripheral blood activated in the presence of TGFβ upregulated CD103 and secreted CXCL13. Conversely, inhibition of TGFβ receptor signaling abrogated CXCL13 production. CXCL13+CD103+CD8+ TILs correlated with B-cell recruitment, TLSs, and neoantigen burden in six cohorts of human tumors. Altogether, our findings indicated that TGFβ plays a noncanonical role in coordinating immune responses against human tumors and suggest a potential role for CXCL13+CD103+CD8+ TILs in mediating B-cell recruitment and TLS formation in human tumors.

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