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Zhongguo Zhong Yao Za Zhi. 2018 Oct;43(20):4050-4054. doi: 10.19540/j.cnki.cjcmm.2018.0106.

[Establishment of a quantitative real-time PCR for rapid detection of Panax quinquefolius].

[Article in Chinese]

Author information

1
Technology Center of Zhuhai Entry-Exit Inspection and Quarantine Bureau, Zhuhai 519000, China.

Abstract

In this study, the specific primers and probes of Panax quinquefolius were designed for a quantitative real-time PCR, and the rapid identification method of P. quinquefolius was established by optimizing conditions. The method was used to validate 43 samples of the traditional Chinese medicine,and the results showed that 22 samples of P. quinquefolius were identified accurately. The limit of detection of the method can be reach to 1×10⁻⁴ ng. The method is accurate, fast, sensitive and specifically.

KEYWORDS:

Panax ginseng ; Panax quinquefolius ; quantitative real-time PCR ; rapid identification

PMID:
30486529
DOI:
10.19540/j.cnki.cjcmm.2018.0106
[Indexed for MEDLINE]

Conflict of interest statement

The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose.

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