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Appl Environ Microbiol. 2014 Jul;80(14):4383-90. doi: 10.1128/AEM.00045-14. Epub 2014 May 9.

Effect of long-term starvation on the survival, recovery, and carbon utilization profiles of a bovine Escherichia coli O157:H7 isolate from New Zealand.

Author information

1
Food Assurance and Meat Quality, Food & Bio-based Products, Hopkirk Research Institute, AgResearch, Palmerston North, New Zealand Department of Biological Sciences, University of Waikato, Hamilton, New Zealand ron.xavier@mpi.govt.nz.
2
Department of Biological Sciences, University of Waikato, Hamilton, New Zealand.
3
Ministry of Primary Industries, Wellington, New Zealand.

Abstract

The ability to maintain a dual lifestyle of colonizing the ruminant gut and surviving in nonhost environments once shed is key to the success of Escherichia coli O157:H7 as a zoonotic pathogen. Both physical and biological conditions encountered by the bacteria are likely to change during the transition between host and nonhost environments. In this study, carbon starvation at suboptimal temperatures in nonhost environments was simulated by starving a New Zealand bovine E. coli O157:H7 isolate in phosphate-buffered saline at 4 and 15°C for 84 days. Recovery of starved cells on media with different nutrient availabilities was monitored under aerobic and anaerobic conditions. We found that the New Zealand bovine E. coli O157:H7 isolate was able to maintain membrane integrity and viability over 84 days and that the level of recovery depended on the nutrient level of the recovery medium as well as the starvation temperature. In addition, a significant difference in carbon utilization was observed between starved and nonstarved cells.

PMID:
24814789
PMCID:
PMC4068667
DOI:
10.1128/AEM.00045-14
[Indexed for MEDLINE]
Free PMC Article

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