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Items: 1 to 50 of 184

1.

Validation of Reactivity Descriptors to Assess the Aromatic Stacking within the Tyrosine Gate of FimH.

Roos G, Wellens A, Touaibia M, Yamakawa N, Geerlings P, Roy R, Wyns L, Bouckaert J.

ACS Med Chem Lett. 2013 Sep 13;4(11):1085-90. doi: 10.1021/ml400269v. eCollection 2013 Nov 14.

2.

Study of the structural and dynamic effects in the FimH adhesin upon α-d-heptyl mannose binding.

Vanwetswinkel S, Volkov AN, Sterckx YG, Garcia-Pino A, Buts L, Vranken WF, Bouckaert J, Roy R, Wyns L, van Nuland NA.

J Med Chem. 2014 Feb 27;57(4):1416-27. doi: 10.1021/jm401666c. Epub 2014 Feb 7.

PMID:
24476493
3.

A nanobody binding to non-amyloidogenic regions of the protein human lysozyme enhances partial unfolding but inhibits amyloid fibril formation.

De Genst E, Chan PH, Pardon E, Hsu SD, Kumita JR, Christodoulou J, Menzer L, Chirgadze DY, Robinson CV, Muyldermans S, Matagne A, Wyns L, Dobson CM, Dumoulin M.

J Phys Chem B. 2013 Oct 24;117(42):13245-13258. doi: 10.1021/jp403425z. Epub 2013 Sep 24.

4.

Nanobodies raised against monomeric α-synuclein distinguish between fibrils at different maturation stages.

Guilliams T, El-Turk F, Buell AK, O'Day EM, Aprile FA, Esbjörner EK, Vendruscolo M, Cremades N, Pardon E, Wyns L, Welland ME, Steyaert J, Christodoulou J, Dobson CM, De Genst E.

J Mol Biol. 2013 Jul 24;425(14):2397-411. doi: 10.1016/j.jmb.2013.01.040. Epub 2013 Apr 1.

5.

Atomic structure of a nanobody-trapped domain-swapped dimer of an amyloidogenic beta2-microglobulin variant.

Domanska K, Vanderhaegen S, Srinivasan V, Pardon E, Dupeux F, Marquez JA, Giorgetti S, Stoppini M, Wyns L, Bellotti V, Steyaert J.

Proc Natl Acad Sci U S A. 2011 Jan 25;108(4):1314-9. doi: 10.1073/pnas.1008560108. Epub 2011 Jan 10.

6.

Structure and properties of a complex of α-synuclein and a single-domain camelid antibody.

De Genst EJ, Guilliams T, Wellens J, O'Day EM, Waudby CA, Meehan S, Dumoulin M, Hsu ST, Cremades N, Verschueren KH, Pardon E, Wyns L, Steyaert J, Christodoulou J, Dobson CM.

J Mol Biol. 2010 Sep 17;402(2):326-43. doi: 10.1016/j.jmb.2010.07.001. Epub 2010 Jul 8.

7.

Allostery and intrinsic disorder mediate transcription regulation by conditional cooperativity.

Garcia-Pino A, Balasubramanian S, Wyns L, Gazit E, De Greve H, Magnuson RD, Charlier D, van Nuland NA, Loris R.

Cell. 2010 Jul 9;142(1):101-11. doi: 10.1016/j.cell.2010.05.039.

8.

Structural and thermodynamic characterization of pre- and postpolymerization states in the F4 fimbrial subunit FaeG.

Van Molle I, Moonens K, Garcia-Pino A, Buts L, De Kerpel M, Wyns L, Bouckaert J, De Greve H.

J Mol Biol. 2009 Dec 18;394(5):957-67. doi: 10.1016/j.jmb.2009.09.059. Epub 2009 Sep 30.

PMID:
19799915
9.

(1)H, (13)C and (15)N assignments of a camelid nanobody directed against human alpha-synuclein.

Vuchelen A, O'Day E, De Genst E, Pardon E, Wyns L, Dumoulin M, Dobson CM, Christodoulou J, Hsu ST.

Biomol NMR Assign. 2009 Dec;3(2):231-3. doi: 10.1007/s12104-009-9182-4.

10.

How thioredoxin dissociates its mixed disulfide.

Roos G, Foloppe N, Van Laer K, Wyns L, Nilsson L, Geerlings P, Messens J.

PLoS Comput Biol. 2009 Aug;5(8):e1000461. doi: 10.1371/journal.pcbi.1000461. Epub 2009 Aug 13.

11.

Rejuvenation of CcdB-poisoned gyrase by an intrinsically disordered protein domain.

De Jonge N, Garcia-Pino A, Buts L, Haesaerts S, Charlier D, Zangger K, Wyns L, De Greve H, Loris R.

Mol Cell. 2009 Jul 31;35(2):154-63. doi: 10.1016/j.molcel.2009.05.025.

12.

Sequence-specific 1H, 15N and 13C resonance assignments of the 23.7-kDa homodimeric toxin CcdB from Vibrio fischeri.

Respondek M, Buts L, De Jonge N, Haesaerts S, Loris R, Van Melderen L, Wyns L, Zangger K.

Biomol NMR Assign. 2009 Jun;3(1):145-7. doi: 10.1007/s12104-009-9161-9. Epub 2009 Apr 3.

PMID:
19636967
13.

The F4 fimbrial chaperone FaeE is stable as a monomer that does not require self-capping of its pilin-interactive surfaces.

Van Molle I, Moonens K, Buts L, Garcia-Pino A, Panjikar S, Wyns L, De Greve H, Bouckaert J.

Acta Crystallogr D Biol Crystallogr. 2009 May;65(Pt 5):411-20. doi: 10.1107/S0907444909005174. Epub 2009 Apr 18.

PMID:
19390146
14.

Arsenate reductase, mycothiol, and mycoredoxin concert thiol/disulfide exchange.

Ordóñez E, Van Belle K, Roos G, De Galan S, Letek M, Gil JA, Wyns L, Mateos LM, Messens J.

J Biol Chem. 2009 May 29;284(22):15107-16. doi: 10.1074/jbc.M900877200. Epub 2009 Mar 13.

15.

Coupling of domain swapping to kinetic stability in a thioredoxin mutant.

Garcia-Pino A, Martinez-Rodriguez S, Wahni K, Wyns L, Loris R, Messens J.

J Mol Biol. 2009 Feb 6;385(5):1590-9. doi: 10.1016/j.jmb.2008.11.040. Epub 2008 Dec 3.

PMID:
19071139
16.

Camelid immunoglobulins and nanobody technology.

Muyldermans S, Baral TN, Retamozzo VC, De Baetselier P, De Genst E, Kinne J, Leonhardt H, Magez S, Nguyen VK, Revets H, Rothbauer U, Stijlemans B, Tillib S, Wernery U, Wyns L, Hassanzadeh-Ghassabeh G, Saerens D.

Vet Immunol Immunopathol. 2009 Mar 15;128(1-3):178-83. doi: 10.1016/j.vetimm.2008.10.299. Epub 2008 Oct 17.

PMID:
19026455
17.

Crystallization of Doc and the Phd-Doc toxin-antitoxin complex.

Garcia-Pino A, Dao-Thi MH, Gazit E, Magnuson RD, Wyns L, Loris R.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Nov 1;64(Pt 11):1034-8. doi: 10.1107/S1744309108031722. Epub 2008 Oct 28.

18.

Engineering a camelid antibody fragment that binds to the active site of human lysozyme and inhibits its conversion into amyloid fibrils.

Chan PH, Pardon E, Menzer L, De Genst E, Kumita JR, Christodoulou J, Saerens D, Brans A, Bouillenne F, Archer DB, Robinson CV, Muyldermans S, Matagne A, Redfield C, Wyns L, Dobson CM, Dumoulin M.

Biochemistry. 2008 Oct 21;47(42):11041-54. doi: 10.1021/bi8005797. Epub 2008 Sep 25.

PMID:
18816062
19.

Doc of prophage P1 is inhibited by its antitoxin partner Phd through fold complementation.

Garcia-Pino A, Christensen-Dalsgaard M, Wyns L, Yarmolinsky M, Magnuson RD, Gerdes K, Loris R.

J Biol Chem. 2008 Nov 7;283(45):30821-7. doi: 10.1074/jbc.M805654200. Epub 2008 Aug 30.

20.

Heterologous expression, purification and characterisation of the extracellular domain of trypanosome invariant surface glycoprotein ISG75.

Tran T, Büscher P, Vandenbussche G, Wyns L, Messens J, De Greve H.

J Biotechnol. 2008 Jun 30;135(3):247-54. doi: 10.1016/j.jbiotec.2008.04.012. Epub 2008 May 1.

PMID:
18538880
21.

Intervening with urinary tract infections using anti-adhesives based on the crystal structure of the FimH-oligomannose-3 complex.

Wellens A, Garofalo C, Nguyen H, Van Gerven N, Slättegård R, Hernalsteens JP, Wyns L, Oscarson S, De Greve H, Hultgren S, Bouckaert J.

PLoS One. 2008 Apr 30;3(4):e2040. doi: 10.1371/journal.pone.0002040. Erratum in: PLoS ONE. 2008;3(6). doi: 10.1371/annotation/ea59d179-0a71-4836-86f0-8d375f5df089.

22.

Nonspecific base recognition mediated by water bridges and hydrophobic stacking in ribonuclease I from Escherichia coli.

Rodriguez SM, Panjikar S, Van Belle K, Wyns L, Messens J, Loris R.

Protein Sci. 2008 Apr;17(4):681-90. doi: 10.1110/ps.073420708. Epub 2008 Feb 27.

23.

Combining sites of bacterial fimbriae.

De Greve H, Wyns L, Bouckaert J.

Curr Opin Struct Biol. 2007 Oct;17(5):506-12. Epub 2007 Aug 23. Review.

PMID:
17719218
24.

The oxidase DsbA folds a protein with a nonconsecutive disulfide.

Messens J, Collet JF, Van Belle K, Brosens E, Loris R, Wyns L.

J Biol Chem. 2007 Oct 26;282(43):31302-7. Epub 2007 Aug 16.

25.

How a plant lectin recognizes high mannose oligosaccharides.

Garcia-Pino A, Buts L, Wyns L, Imberty A, Loris R.

Plant Physiol. 2007 Aug;144(4):1733-41. Epub 2007 Jun 7.

26.

Purification and crystallization of Vibrio fischeri CcdB and its complexes with fragments of gyrase and CcdA.

De Jonge N, Buts L, Vangelooven J, Mine N, Van Melderen L, Wyns L, Loris R.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Apr 1;63(Pt 4):356-60. Epub 2007 Mar 30.

27.

The conserved active site proline determines the reducing power of Staphylococcus aureus thioredoxin.

Roos G, Garcia-Pino A, Van Belle K, Brosens E, Wahni K, Vandenbussche G, Wyns L, Loris R, Messens J.

J Mol Biol. 2007 May 4;368(3):800-11. Epub 2007 Feb 22.

PMID:
17368484
28.

Chloroplasts assemble the major subunit FaeG of Escherichia coli F4 (K88) fimbriae to strand-swapped dimers.

Van Molle I, Joensuu JJ, Buts L, Panjikar S, Kotiaho M, Bouckaert J, Wyns L, Niklander-Teeri V, De Greve H.

J Mol Biol. 2007 May 4;368(3):791-9. Epub 2007 Feb 22.

PMID:
17368480
29.

Crystallization of ornithine acetyltransferase from yeast by counter-diffusion and preliminary X-ray study.

Maes D, Crabeel M, Van de Weerdt C, Martial J, Peeters E, Charlier D, Decanniere K, Vanhee C, Wyns L, Zegers I.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Dec 1;62(Pt 12):1294-7. Epub 2006 Nov 30.

30.

N-terminal truncation enables crystallization of the receptor-binding domain of the FedF bacterial adhesin.

De Kerpel M, Van Molle I, Brys L, Wyns L, De Greve H, Bouckaert J.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Dec 1;62(Pt 12):1278-82. Epub 2006 Nov 30.

31.

Combining site-specific mutagenesis and seeding as a strategy to crystallize 'difficult' proteins: the case of Staphylococcus aureus thioredoxin.

Roos G, Brosens E, Wahni K, Desmyter A, Spinelli S, Wyns L, Messens J, Loris R.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Dec 1;62(Pt 12):1255-8. Epub 2006 Nov 30.

32.

Crystallization and preliminary X-ray analysis of a protease inhibitor from the latex of Carica papaya.

Azarkan M, Garcia-Pino A, Dibiani R, Wyns L, Loris R, Baeyens-Volant D.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Dec 1;62(Pt 12):1239-42. Epub 2006 Nov 30.

33.

The affinity of the FimH fimbrial adhesin is receptor-driven and quasi-independent of Escherichia coli pathotypes.

Bouckaert J, Mackenzie J, de Paz JL, Chipwaza B, Choudhury D, Zavialov A, Mannerstedt K, Anderson J, Piérard D, Wyns L, Seeberger PH, Oscarson S, De Greve H, Knight SD.

Mol Microbiol. 2006 Sep;61(6):1556-68. Epub 2006 Aug 23.

34.

Interplay between metal binding and cis/trans isomerization in legume lectins: structural and thermodynamic study of P. angolensis lectin.

Garcia-Pino A, Buts L, Wyns L, Loris R.

J Mol Biol. 2006 Aug 4;361(1):153-67. Epub 2006 Jun 21.

PMID:
16824540
35.

Interplay between ion binding and catalysis in the thioredoxin-coupled arsenate reductase family.

Roos G, Buts L, Van Belle K, Brosens E, Geerlings P, Loris R, Wyns L, Messens J.

J Mol Biol. 2006 Jul 21;360(4):826-38. Epub 2006 Jun 6.

PMID:
16797027
36.

Structural basis for the recognition of complex-type biantennary oligosaccharides by Pterocarpus angolensis lectin.

Buts L, Garcia-Pino A, Imberty A, Amiot N, Boons GJ, Beeckmans S, Versées W, Wyns L, Loris R.

FEBS J. 2006 Jun;273(11):2407-20.

37.

The activation of electrophile, nucleophile and leaving group during the reaction catalysed by pI258 arsenate reductase.

Roos G, Loverix S, Brosens E, Van Belle K, Wyns L, Geerlings P, Messens J.

Chembiochem. 2006 Jun;7(6):981-9.

PMID:
16607668
38.

Structural basis of carbohydrate recognition by a Man(alpha1-2)Man-specific lectin from Bowringia milbraedii.

Buts L, Garcia-Pino A, Wyns L, Loris R.

Glycobiology. 2006 Jul;16(7):635-40. Epub 2006 Mar 27.

PMID:
16567368
39.

Molecular basis for the preferential cleft recognition by dromedary heavy-chain antibodies.

De Genst E, Silence K, Decanniere K, Conrath K, Loris R, Kinne J, Muyldermans S, Wyns L.

Proc Natl Acad Sci U S A. 2006 Mar 21;103(12):4586-91. Epub 2006 Mar 13.

40.

Structure of a membrane-based steric chaperone in complex with its lipase substrate.

Pauwels K, Lustig A, Wyns L, Tommassen J, Savvides SN, Van Gelder P.

Nat Struct Mol Biol. 2006 Apr;13(4):374-5. Epub 2006 Mar 5.

PMID:
16518399
41.

Crystallization of the C-terminal domain of the addiction antidote CcdA in complex with its toxin CcdB.

Buts L, De Jonge N, Loris R, Wyns L, Dao-Thi MH.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Oct 1;61(Pt 10):949-52. Epub 2005 Sep 30.

42.

Crystallization and preliminary X-ray analysis of the Man(alpha1-2)Man-specific lectin from Bowringia mildbraedii in complex with its carbohydrate ligand.

Garcia-Pino A, Loris R, Wyns L, Buts L.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Oct 1;61(Pt 10):931-4. Epub 2005 Sep 30.

43.

Crystallization and crystal manipulation of a steric chaperone in complex with its lipase substrate.

Pauwels K, Loris R, Vandenbussche G, Ruysschaert JM, Wyns L, Van Gelder P.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Aug 1;61(Pt 8):791-5. Epub 2005 Jul 30.

44.

Crystallization of the FaeE chaperone of Escherichia coli F4 fimbriae.

Van Molle I, Buts L, Coppens F, Qiang L, Wyns L, Loris R, Bouckaert J, De Greve H.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Apr 1;61(Pt 4):427-31. Epub 2005 Apr 1.

45.

Toxin-antitoxin modules as bacterial metabolic stress managers.

Buts L, Lah J, Dao-Thi MH, Wyns L, Loris R.

Trends Biochem Sci. 2005 Dec;30(12):672-9. Epub 2005 Oct 28. Review.

PMID:
16257530
46.

Protein crystallization in hydrogel beads.

Willaert R, Zegers I, Wyns L, Sleutel M.

Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1280-8. Epub 2005 Aug 16.

PMID:
16131762
47.

Identification of a universal VHH framework to graft non-canonical antigen-binding loops of camel single-domain antibodies.

Saerens D, Pellis M, Loris R, Pardon E, Dumoulin M, Matagne A, Wyns L, Muyldermans S, Conrath K.

J Mol Biol. 2005 Sep 23;352(3):597-607.

PMID:
16095608
48.

Impact of natural variation in bacterial F17G adhesins on crystallization behaviour.

Buts L, Wellens A, Van Molle I, Wyns L, Loris R, Lahmann M, Oscarson S, De Greve H, Bouckaert J.

Acta Crystallogr D Biol Crystallogr. 2005 Aug;61(Pt 8):1149-59. Epub 2005 Jul 20.

PMID:
16041081
49.

Crystallization and crystal manipulation of the Pterocarpus angolensis seed lectin.

Loris R, Garcia-Pino A, Buts L, Bouckaert J, Beeckmans S, De Greve H, Wyns L.

Acta Crystallogr D Biol Crystallogr. 2005 Jun;61(Pt 6):685-9. Epub 2005 May 26.

PMID:
15930620
50.

Antigen binding and solubility effects upon the veneering of a camel VHH in framework-2 to mimic a VH.

Conrath K, Vincke C, Stijlemans B, Schymkowitz J, Decanniere K, Wyns L, Muyldermans S, Loris R.

J Mol Biol. 2005 Jul 1;350(1):112-25.

PMID:
15913651

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