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Items: 38


Abstracts of Presentations at the Association of Clinical Scientists 139th Meeting Hershey, PA, May 15-18, 2019.

Donaldson K, Buchanich JM, Grigson PS, Deneke E, Donaldson K, Vrana KE, Sacks DB, Kuehn GJ, Cardamone D, Pesce A, Smiley S, Nickley J, Krock K, Thomas R, Wilkerson ML, Farag HA, Challa SR, Tice AM, Wolk DM, Prichard J, Grant ML, Regmi S, Kerbacher B, Quinton LE, Farag HA, Tice AM, Wolk DM, Olson J, Haynes A, Yu E, McCully KS, Assi J, Wong M, Zarrin-Khameh N, Nifong TP, Hawker CD, Carlton GT, Rivera JM, Foulis PR, Zuraw A, Morlote D, Peker D, Reddy V, Harada S, Crutchfield C, Zander D, Barbhuiya MA, Pederson EC, Straub ML, Scott SC, Neibauer TL, Salter WF, Creer MH, Zhu Y, Bornhorst JA, Theobald JP, Algeciras-Schimnich A, Cao L, Knox J, Hardy R, Texas HJ, McGuire MF, Hunter RL, Brown RE, Hicks J, Hicks J, Cai Z, Brown RE, Ali Y, Cheng KC, Katz SR, Ding Y, Vanselow DJ, Yakovlev MA, Lin AY, Clark DP, Vargas P, Xin X, Copper JE, Canfield VA, Ang KC, Wang Y, Xiao X, De Carlo F, van Rossum DB, La Rivière PJ, Newell J, Hossler C, Roche M, Warrick J, Phaeton R, Kesterson J, Donaldson K, Myers C, Barrios R, Mintz P, Robyak K, Hamilton C, McGhee P, Pederson C, Straub M, Scott S, Neibauer T, Salter W, Creer M, Zhu Y, Hamilton C, Robyak K, McGhee P, Pederson C, Straub M, Scott S, Neibauer T, Salter W, Creer M, Zhu Y, Singh N, Morlote D, Vnencak-Jones C, Yemelyanova A, Harada S, Shah M, Moghadamtousi SZ, Lan C, Duose D, Hu P, Esquenazi Y, Luthra R, Ballester LY, Koenig AN, Liu CG, Zhang J, Kalia A, Al-Habib A, Van Arsdall M, Dhingra S, Patel K, Tatevian N.

Ann Clin Lab Sci. 2019 May;49(3):403-416. No abstract available.


Performance of the T2Bacteria Panel for Diagnosing Bloodstream Infections: A Diagnostic Accuracy Study.

Nguyen MH, Clancy CJ, Pasculle AW, Pappas PG, Alangaden G, Pankey GA, Schmitt BH, Rasool A, Weinstein MP, Widen R, Hernandez DR, Wolk DM, Walsh TJ, Perfect JR, Wilson MN, Mylonakis E.

Ann Intern Med. 2019 May 14. doi: 10.7326/M18-2772. [Epub ahead of print]


Near point-of-care adoption of Cepheid Xpert® Flu/RSV XC testing within an integrated healthcare delivery network.

Hernandez DR, Pellitteri GP, Kimmich DJ, Heiter BJ, Smeal K, Wolk DM.

Diagn Microbiol Infect Dis. 2019 May;94(1):28-29. doi: 10.1016/j.diagmicrobio.2018.11.013. Epub 2018 Nov 23.


Matrix-Assisted Laser Desorption Time of Flight Mass Spectrometry.

Wolk DM, Clark AE.

Clin Lab Med. 2018 Sep;38(3):471-486. doi: 10.1016/j.cll.2018.05.008. Review.


Multicenter Diagnostic Accuracy Evaluation of the Luminex Aries Real-Time PCR Assay for Group B Streptococcus Detection in Lim Broth-Enriched Samples.

Hernandez DR, Wolk DM, Walker KL, Young S, Dunn R, Dunbar SA, Rao A.

J Clin Microbiol. 2018 Jul 26;56(8). pii: e01768-17. doi: 10.1128/JCM.01768-17. Print 2018 Aug.


Multicenter Evaluation of the Accelerate PhenoTest BC Kit for Rapid Identification and Phenotypic Antimicrobial Susceptibility Testing Using Morphokinetic Cellular Analysis.

Pancholi P, Carroll KC, Buchan BW, Chan RC, Dhiman N, Ford B, Granato PA, Harrington AT, Hernandez DR, Humphries RM, Jindra MR, Ledeboer NA, Miller SA, Mochon AB, Morgan MA, Patel R, Schreckenberger PC, Stamper PD, Simner PJ, Tucci NE, Zimmerman C, Wolk DM.

J Clin Microbiol. 2018 Mar 26;56(4). pii: e01329-17. doi: 10.1128/JCM.01329-17. Print 2018 Apr.


Multicenter Evaluation of the Xpert MRSA NxG Assay for Detection of Methicillin-Resistant Staphylococcus aureus in Nasal Swabs.

Yarbrough ML, Warren DK, Allen K, Burkholder D, Daum R, Donskey C, Knaack D, LaMarca A, May L, Miller LG, Parenti DM, Peterson L, Tan TY, Widen R, Hernandez DR, Wolk DM, Burnham CA.

J Clin Microbiol. 2017 Dec 26;56(1). pii: e01381-17. doi: 10.1128/JCM.01381-17. Print 2018 Jan.


16S rRNA gene sequencing on a benchtop sequencer: accuracy for identification of clinically important bacteria.

Watts GS, Youens-Clark K, Slepian MJ, Wolk DM, Oshiro MM, Metzger GS, Dhingra D, Cranmer LD, Hurwitz BL.

J Appl Microbiol. 2017 Dec;123(6):1584-1596. doi: 10.1111/jam.13590. Epub 2017 Nov 7.


Cladophialophora Bantiana : A Rare Intracerebral Fungal Abscess-Case Series and Review of Literature.

Ahmad M, Jacobs D, Wu HH, Wolk DM, Kazmi SAJ, Jaramillo C, Toms SA.

Surg J (N Y). 2017 Mar 30;3(2):e62-e68. doi: 10.1055/s-0037-1598248. eCollection 2017 Apr.


Bloodstream Infections.

Martinez RM, Wolk DM.

Microbiol Spectr. 2016 Aug;4(4). doi: 10.1128/microbiolspec.DMIH2-0031-2016. Review.


Effectiveness of Practices To Increase Timeliness of Providing Targeted Therapy for Inpatients with Bloodstream Infections: a Laboratory Medicine Best Practices Systematic Review and Meta-analysis.

Buehler SS, Madison B, Snyder SR, Derzon JH, Cornish NE, Saubolle MA, Weissfeld AS, Weinstein MP, Liebow EB, Wolk DM.

Clin Microbiol Rev. 2016 Jan;29(1):59-103. doi: 10.1128/CMR.00053-14. Review.


Multicenter Evaluation of MRSASelect II Chromogenic Agar for Identification of Methicillin-Resistant Staphylococcus aureus from Wound and Nasal Specimens.

Hernandez DR, Newton DW, Ledeboer NA, Buchan B, Young C, Clark AE, Connoly J, Wolk DM.

J Clin Microbiol. 2016 Feb;54(2):305-11. doi: 10.1128/JCM.02410-15. Epub 2015 Nov 18.


T2MR and T2Candida: novel technology for the rapid diagnosis of candidemia and invasive candidiasis.

Pfaller MA, Wolk DM, Lowery TJ.

Future Microbiol. 2016;11(1):103-17. doi: 10.2217/fmb.15.111. Epub 2015 Sep 15. Review.


Physician documentation of sepsis syndrome is associated with more aggressive treatment.

Stoneking LR, Winkler JP, DeLuca LA, Stolz U, Stutz A, Luman JC, Gaub M, Wolk DM, Fiorello AB, Denninghoff KR.

West J Emerg Med. 2015 May;16(3):401-7. doi: 10.5811/westjem.2015.3.25529. Epub 2015 Apr 10.


Update on the diagnosis of pulmonary coccidioidomycosis.

Malo J, Luraschi-Monjagatta C, Wolk DM, Thompson R, Hage CA, Knox KS.

Ann Am Thorac Soc. 2014 Feb;11(2):243-53. doi: 10.1513/AnnalsATS.201308-286FR. Review.


Multicenter evaluation of the Sepsityper™ extraction kit and MALDI-TOF MS for direct identification of positive blood culture isolates using the BD BACTEC™ FX and VersaTREK(®) diagnostic blood culture systems.

Schieffer KM, Tan KE, Stamper PD, Somogyi A, Andrea SB, Wakefield T, Romagnoli M, Chapin KC, Wolk DM, Carroll KC.

J Appl Microbiol. 2014 Apr;116(4):934-41. doi: 10.1111/jam.12434. Epub 2014 Feb 8.


Matrix-assisted laser desorption ionization-time of flight mass spectrometry: a fundamental shift in the routine practice of clinical microbiology.

Clark AE, Kaleta EJ, Arora A, Wolk DM.

Clin Microbiol Rev. 2013 Jul;26(3):547-603. doi: 10.1128/CMR.00072-12. Review.


Molecular Characterization of Invasive Staphylococcus aureus Infection in Central New York Children: Importance of Two Clonal Groups and Inconsistent Presence of Selected Virulence Determinants.

Suryadevara M, Clark AE, Wolk DM, Carman A, Rosenbaum PF, Shaw J.

J Pediatric Infect Dis Soc. 2013 Mar;2(1):30-9. doi: 10.1093/jpids/pis087. Epub 2012 Sep 18.


Would earlier microbe identification alter antibiotic therapy in bacteremic emergency department patients?

Stoneking LR, Patanwala AE, Winkler JP, Fiorello AB, Lee ES, Olson DP, Wolk DM.

J Emerg Med. 2013 Jan;44(1):1-8. doi: 10.1016/j.jemermed.2012.02.036. Epub 2012 May 16.


PCR-electrospray ionization mass spectrometry: the potential to change infectious disease diagnostics in clinical and public health laboratories.

Wolk DM, Kaleta EJ, Wysocki VH.

J Mol Diagn. 2012 Jul;14(4):295-304. doi: 10.1016/j.jmoldx.2012.02.005. Epub 2012 May 11. Review.


Detection of respiratory viruses on air filters from aircraft.

Korves TM, Johnson D, Jones BW, Watson J, Wolk DM, Hwang GM.

Lett Appl Microbiol. 2011 Sep;53(3):306-12. doi: 10.1111/j.1472-765X.2011.03107.x. Epub 2011 Jul 14.


Comparative analysis of PCR-electrospray ionization/mass spectrometry (MS) and MALDI-TOF/MS for the identification of bacteria and yeast from positive blood culture bottles.

Kaleta EJ, Clark AE, Cherkaoui A, Wysocki VH, Ingram EL, Schrenzel J, Wolk DM.

Clin Chem. 2011 Jul;57(7):1057-67. doi: 10.1373/clinchem.2011.161968. Epub 2011 May 16.


Diagnostic accuracy of the Cepheid GeneXpert vanA/vanB assay ver. 1.0 to detect the vanA and vanB vancomycin resistance genes in Enterococcus from perianal specimens.

Marner ES, Wolk DM, Carr J, Hewitt C, Dominguez LL, Kovacs T, Johnson DR, Hayden RT.

Diagn Microbiol Infect Dis. 2011 Apr;69(4):382-9. doi: 10.1016/j.diagmicrobio.2010.11.005.


Use of PCR coupled with electrospray ionization mass spectrometry for rapid identification of bacterial and yeast bloodstream pathogens from blood culture bottles.

Kaleta EJ, Clark AE, Johnson DR, Gamage DC, Wysocki VH, Cherkaoui A, Schrenzel J, Wolk DM.

J Clin Microbiol. 2011 Jan;49(1):345-53. doi: 10.1128/JCM.00936-10. Epub 2010 Nov 3.


Non-traumatic Alternaria keratomycosis in a rigid gas-permeable contact lens patient.

Ursea R, Tavares LA, Feng MT, McColgin AZ, Snyder RW, Wolk DM.

Br J Ophthalmol. 2010 Mar;94(3):389-90. doi: 10.1136/bjo.2009.164632. No abstract available.


Diabetic foot infections: A need for innovative assessments.

Fisher TK, Wolcott R, Wolk DM, Bharara M, Kimbriel HR, Armstrong DG.

Int J Low Extrem Wounds. 2010 Mar;9(1):31-6. doi: 10.1177/1534734610363459.


Pathogen profiling: rapid molecular characterization of Staphylococcus aureus by PCR/electrospray ionization-mass spectrometry and correlation with phenotype.

Wolk DM, Blyn LB, Hall TA, Sampath R, Ranken R, Ivy C, Melton R, Matthews H, White N, Li F, Harpin V, Ecker DJ, Limbago B, McDougal LK, Wysocki VH, Cai M, Carroll KC.

J Clin Microbiol. 2009 Oct;47(10):3129-37. doi: 10.1128/JCM.00709-09. Epub 2009 Aug 26.


Rapid molecular genotyping and clonal complex assignment of Staphylococcus aureus isolates by PCR coupled to electrospray ionization-mass spectrometry.

Hall TA, Sampath R, Blyn LB, Ranken R, Ivy C, Melton R, Matthews H, White N, Li F, Harpin V, Ecker DJ, McDougal LK, Limbago B, Ross T, Wolk DM, Wysocki V, Carroll KC.

J Clin Microbiol. 2009 Jun;47(6):1733-41. doi: 10.1128/JCM.02175-08. Epub 2009 Mar 18.


Rapid detection of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in wound specimens and blood cultures: multicenter preclinical evaluation of the Cepheid Xpert MRSA/SA skin and soft tissue and blood culture assays.

Wolk DM, Struelens MJ, Pancholi P, Davis T, Della-Latta P, Fuller D, Picton E, Dickenson R, Denis O, Johnson D, Chapin K.

J Clin Microbiol. 2009 Mar;47(3):823-6. doi: 10.1128/JCM.01884-08. Epub 2009 Jan 14.


Multicenter evaluation of the Cepheid Xpert methicillin-resistant Staphylococcus aureus (MRSA) test as a rapid screening method for detection of MRSA in nares.

Wolk DM, Picton E, Johnson D, Davis T, Pancholi P, Ginocchio CC, Finegold S, Welch DF, de Boer M, Fuller D, Solomon MC, Rogers B, Mehta MS, Peterson LR.

J Clin Microbiol. 2009 Mar;47(3):758-64. doi: 10.1128/JCM.01714-08. Epub 2009 Jan 7.


Development of a method for the detection of waterborne microsporidia.

Hoffman RM, Wolk DM, Spencer SK, Borchardt MA.

J Microbiol Methods. 2007 Aug;70(2):312-8. Epub 2007 May 21.


Is your document control out of control? Complying with document control regulations.

Garcia F, Harrison E, Wise C, Wolk DM.

Clin Leadersh Manag Rev. 2003 Sep-Oct;17(5):255-62.


In situ hybridization for the differentiation of Aspergillus, Fusarium, and Pseudallescheria species in tissue section.

Hayden RT, Isotalo PA, Parrett T, Wolk DM, Qian X, Roberts GD, Lloyd RV.

Diagn Mol Pathol. 2003 Mar;12(1):21-6.


Real-time PCR method for detection of Encephalitozoon intestinalis from stool specimens.

Wolk DM, Schneider SK, Wengenack NL, Sloan LM, Rosenblatt JE.

J Clin Microbiol. 2002 Nov;40(11):3922-8.


Laboratory diagnosis of viral hepatitis.

Wolk DM, Jones MF, Rosenblatt JE.

Infect Dis Clin North Am. 2001 Dec;15(4):1109-26. Review.


Fatal sepsis in a patient with rheumatoid arthritis treated with etanercept.

Baghai M, Osmon DR, Wolk DM, Wold LE, Haidukewych GJ, Matteson EL.

Mayo Clin Proc. 2001 Jun;76(6):653-6.


A spore counting method and cell culture model for chlorine disinfection studies of Encephalitozoon syn. Septata intestinalis.

Wolk DM, Johnson CH, Rice EW, Marshall MM, Grahn KF, Plummer CB, Sterling CR.

Appl Environ Microbiol. 2000 Apr;66(4):1266-73.

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