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Integrated Extreme Real-Time PCR and High-Speed Melting Analysis in 52 to 87 Seconds.

Myrick JT, Pryor RJ, Palais RA, Ison SJ, Sanford L, Dwight ZL, Huuskonen JJ, Sundberg SO, Wittwer CT.

Clin Chem. 2019 Feb;65(2):263-271. doi: 10.1373/clinchem.2018.296608. Epub 2018 Nov 20.


A "Culture" Shift: Broad Bacterial Detection, Identification, and Antimicrobial Susceptibility Testing Directly from Whole Blood.

Andini N, Hu A, Zhou L, Cogill S, Wang TH, Wittwer CT, Yang S.

Clin Chem. 2018 Oct;64(10):1453-1462. doi: 10.1373/clinchem.2018.290189. Epub 2018 Aug 7.


The Effect of Single Mismatches on Primer Extension.

Rejali NA, Moric E, Wittwer CT.

Clin Chem. 2018 May;64(5):801-809. doi: 10.1373/clinchem.2017.282285. Epub 2018 Feb 14.


Quantifying variant differences in DNA melting curves: Effects of length, melting rate, and curve overlay.

Li M, Palais RA, Zhou L, Wittwer CT.

Anal Biochem. 2017 Dec 15;539:90-95. doi: 10.1016/j.ab.2017.10.015. Epub 2017 Oct 21.


High-Speed Melting Analysis: The Effect of Melting Rate on Small Amplicon Microfluidic Genotyping.

Pryor RJ, Myrick JT, Palais RA, Sundberg SO, Paek JY, Wittwer CT, Knight IT.

Clin Chem. 2017 Oct;63(10):1624-1632. doi: 10.1373/clinchem.2017.276147. Epub 2017 Aug 17.


MIQE: A Step Toward More Robust and Reproducible Quantitative PCR.

Bustin SA, Wittwer CT.

Clin Chem. 2017 Sep;63(9):1537-1538. doi: 10.1373/clinchem.2016.268953. Epub 2017 Jun 12. Review. No abstract available.


Gain-of-function EGLN1 prolyl hydroxylase (PHD2 D4E:C127S) in combination with EPAS1 (HIF-2α) polymorphism lowers hemoglobin concentration in Tibetan highlanders.

Tashi T, Scott Reading N, Wuren T, Zhang X, Moore LG, Hu H, Tang F, Shestakova A, Lorenzo F, Burjanivova T, Koul P, Guchhait P, Wittwer CT, Julian CG, Shah B, Huff CD, Gordeuk VR, Prchal JT, Ge R.

J Mol Med (Berl). 2017 Jun;95(6):665-670. doi: 10.1007/s00109-017-1519-3. Epub 2017 Feb 23.


Democratizing the Real-Time PCR.

Wittwer CT.

Clin Chem. 2017 Apr;63(4):924-925. doi: 10.1373/clinchem.2016.263269. Epub 2017 Feb 3. No abstract available.


Portable Nanopore Sequencing for Viral Surveillance.

Wittwer CT.

Clin Chem. 2016 Nov;62(11):1427-1429. Epub 2016 Sep 16. No abstract available.


Genomic Copy Number Analysis of HER2-Equivocal Breast Cancers.

Geiersbach KB, Willmore-Payne C, Pasi AV, Paxton CN, Werner TL, Xu X, Wittwer CT, Gulbahce HE, Downs-Kelly E.

Am J Clin Pathol. 2016 Oct;146(4):439-47. doi: 10.1093/ajcp/aqw130. Epub 2016 Sep 10.


Disruptive Innovation in Laboratory Medicine.

Rifai N, Topol E, Chan E, Lo YM, Wittwer CT.

Clin Chem. 2015 Sep;61(9):1129-32. doi: 10.1373/clinchem.2015.243667. Epub 2015 Jul 7. No abstract available.


Detection of chromosomal translocations in formalin-fixed paraffin-embedded (FFPE) leukemic specimens by digital expression profiling.

Liew M, Mao R, Wittwer CT, Salama ME.

Int J Lab Hematol. 2015 Oct;37(5):690-8. doi: 10.1111/ijlh.12388. Epub 2015 Jun 3.


Copy number assessment by competitive PCR with limiting deoxynucleotide triphosphates and high-resolution melting.

Zhou L, Palais RA, Paxton CN, Geiersbach KB, Wittwer CT.

Clin Chem. 2015 May;61(5):724-33. doi: 10.1373/clinchem.2014.236208. Epub 2015 Mar 10.


Rare event counting of CD59- red cells in human blood: A 47-month experience using PNH consensus guidelines for WBC and RBC testing in a reference lab.

Liew M, Farley M, Andreasen J, Parker CJ, Wittwer CT.

Cytometry B Clin Cytom. 2015 Jul-Aug;88(4):261-9. doi: 10.1002/cyto.b.21225. Epub 2015 Feb 3.


Molecular diagnostics: a revolution in progress.

Chiu RW, Lo YM, Wittwer CT.

Clin Chem. 2015 Jan;61(1):1-3. doi: 10.1373/clinchem.2014.233361. No abstract available.


Extreme PCR: efficient and specific DNA amplification in 15-60 seconds.

Farrar JS, Wittwer CT.

Clin Chem. 2015 Jan;61(1):145-53. doi: 10.1373/clinchem.2014.228304. Epub 2014 Oct 15.


Microfluidic genotyping by rapid serial PCR and high-speed melting analysis.

Sundberg SO, Wittwer CT, Howell RM, Huuskonen J, Pryor RJ, Farrar JS, Stiles HM, Palais RA, Knight IT.

Clin Chem. 2014 Oct;60(10):1306-13. doi: 10.1373/clinchem.2014.223768. Epub 2014 Aug 12.


Quasi-digital PCR: Enrichment and quantification of rare DNA variants.

Sundberg SO, Wittwer CT, Zhou L, Palais R, Dwight Z, Gale BK.

Biomed Microdevices. 2014 Aug;16(4):639-44. doi: 10.1007/s10544-014-9866-0.


Genotyping accuracy of high-resolution DNA melting instruments.

Li M, Zhou L, Palais RA, Wittwer CT.

Clin Chem. 2014 Jun;60(6):864-72. doi: 10.1373/clinchem.2013.220160. Epub 2014 Apr 9.


The influence of nucleotide sequence and temperature on the activity of thermostable DNA polymerases.

Montgomery JL, Rejali N, Wittwer CT.

J Mol Diagn. 2014 May;16(3):305-13. doi: 10.1016/j.jmoldx.2014.01.006. Epub 2014 Mar 6.


Heterozygote PCR product melting curve prediction.

Dwight ZL, Palais R, Kent J, Wittwer CT.

Hum Mutat. 2014 Mar;35(3):278-82. doi: 10.1002/humu.22494. Epub 2014 Jan 3.


Fluorescence-based temperature control for polymerase chain reaction.

Sanford LN, Wittwer CT.

Anal Biochem. 2014 Mar 1;448:75-81. doi: 10.1016/j.ab.2013.11.027. Epub 2013 Nov 28.


The need for transparency and good practices in the qPCR literature.

Bustin SA, Benes V, Garson J, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley G, Wittwer CT, Schjerling P, Day PJ, Abreu M, Aguado B, Beaulieu JF, Beckers A, Bogaert S, Browne JA, Carrasco-Ramiro F, Ceelen L, Ciborowski K, Cornillie P, Coulon S, Cuypers A, De Brouwer S, De Ceuninck L, De Craene J, De Naeyer H, De Spiegelaere W, Deckers K, Dheedene A, Durinck K, Ferreira-Teixeira M, Fieuw A, Gallup JM, Gonzalo-Flores S, Goossens K, Heindryckx F, Herring E, Hoenicka H, Icardi L, Jaggi R, Javad F, Karampelias M, Kibenge F, Kibenge M, Kumps C, Lambertz I, Lammens T, Markey A, Messiaen P, Mets E, Morais S, Mudarra-Rubio A, Nakiwala J, Nelis H, Olsvik PA, Pérez-Novo C, Plusquin M, Remans T, Rihani A, Rodrigues-Santos P, Rondou P, Sanders R, Schmidt-Bleek K, Skovgaard K, Smeets K, Tabera L, Toegel S, Van Acker T, Van den Broeck W, Van der Meulen J, Van Gele M, Van Peer G, Van Poucke M, Van Roy N, Vergult S, Wauman J, Tshuikina-Wiklander M, Willems E, Zaccara S, Zeka F, Vandesompele J.

Nat Methods. 2013 Nov;10(11):1063-7. doi: 10.1038/nmeth.2697.


Influence of PCR reagents on DNA polymerase extension rates measured on real-time PCR instruments.

Montgomery JL, Wittwer CT.

Clin Chem. 2014 Feb;60(2):334-40. doi: 10.1373/clinchem.2013.212829. Epub 2013 Oct 15.


Quantum method for fluorescence background removal in DNA melting analysis.

Sanford LN, Kent JO, Wittwer CT.

Anal Chem. 2013 Oct 15;85(20):9907-15. doi: 10.1021/ac4024928. Epub 2013 Sep 26.


Continuous fluorescence monitoring of rapid cycle DNA amplification. 1997.

Wittwer CT, Herrmann MG, Moss AA, Rasmussen RP.

Biotechniques. 2013 Jun;54(6):314-20. No abstract available.


Stopped-flow DNA polymerase assay by continuous monitoring of dNTP incorporation by fluorescence.

Montgomery JL, Rejali N, Wittwer CT.

Anal Biochem. 2013 Oct 15;441(2):133-9. doi: 10.1016/j.ab.2013.07.008. Epub 2013 Jul 16.


High resolution melting analysis of the MMAB gene in cblB patients and in those with undiagnosed methylmalonic aciduria.

Illson ML, Dempsey-Nunez L, Kent J, Huang Q, Brebner A, Raff ML, Watkins D, Gilfix BM, Wittwer CT, Rosenblatt DS.

Mol Genet Metab. 2013 Sep-Oct;110(1-2):86-9. doi: 10.1016/j.ymgme.2013.04.020. Epub 2013 May 4.


The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments.

Huggett JF, Foy CA, Benes V, Emslie K, Garson JA, Haynes R, Hellemans J, Kubista M, Mueller RD, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT, Bustin SA.

Clin Chem. 2013 Jun;59(6):892-902. doi: 10.1373/clinchem.2013.206375. Epub 2013 Apr 9.


Symmetric snapback primers for scanning and genotyping of the cystic fibrosis transmembrane conductance regulator gene.

Zhou L, Palais RA, Ye F, Chen J, Montgomery JL, Wittwer CT.

Clin Chem. 2013 Jul;59(7):1052-61. doi: 10.1373/clinchem.2013.202689. Epub 2013 Mar 15.


Monitoring temperature with fluorescence during real-time PCR and melting analysis.

Sanford LN, Wittwer CT.

Anal Biochem. 2013 Mar 1;434(1):26-33. doi: 10.1016/j.ab.2012.10.037. Epub 2012 Nov 7.


High resolution melting analysis of the MMAA gene in patients with cblA and in those with undiagnosed methylmalonic aciduria.

Dempsey-Nunez L, Illson ML, Kent J, Huang Q, Brebner A, Watkins D, Gilfix BM, Wittwer CT, Rosenblatt DS.

Mol Genet Metab. 2012 Nov;107(3):363-7. doi: 10.1016/j.ymgme.2012.09.012. Epub 2012 Sep 15.


uAnalyze: web-based high-resolution DNA melting analysis with comparison to thermodynamic predictions.

Dwight ZL, Palais R, Wittwer CT.

IEEE/ACM Trans Comput Biol Bioinform. 2012 Nov-Dec;9(6):1805-11.


Rare duplication or deletion of exons 6, 7 and 8 in CYBB leading to X-linked chronic granulomatous disease in two patients from different families.

Stasia MJ, van Leeuwen K, de Boer M, Martel C, Mollin M, Thuret I, Michel G, Hanson C, Augustine NH, Coutton C, Satre V, Wittwer CT, Hill H, Roos D.

J Clin Immunol. 2012 Aug;32(4):653-62. doi: 10.1007/s10875-012-9667-2. Epub 2012 Mar 2.


Advancing laboratory medicine through innovation: a tale of six inventors.

Rifai N, Diamandis EP, Lo YM, Kricka LJ, Wilding P, Ladenson JH, Wittwer CT.

Clin Chem. 2012 Mar;58(3):502-10. doi: 10.1373/clinchem.2011.178582. Epub 2012 Jan 11. No abstract available.


Allele-specific PCR with competitive probe blocking for sensitive and specific detection of BRAF V600E in thyroid fine-needle aspiration specimens.

Smith GD, Zhou L, Rowe LR, Jarboe EA, Collins BT, Bentz JS, Wittwer CT, Chadwick BE.

Acta Cytol. 2011;55(6):576-83. doi: 10.1159/000333453. Epub 2011 Dec 9.


Monovalent and divalent salt correction algorithms for Tm prediction--recommendations for Primer3 usage.

von Ahsen N, Wittwer CT, Schütz E.

Brief Bioinform. 2011 Sep;12(5):514-7. doi: 10.1093/bib/bbq081. Epub 2010 Dec 22.


Detecting and resolving position-dependent temperature effects in real-time quantitative polymerase chain reaction.

von Kanel T, Gerber D, Wittwer CT, Hermann M, Gallati S.

Anal Biochem. 2011 Dec 15;419(2):161-7. doi: 10.1016/j.ab.2011.08.041. Epub 2011 Aug 27.


Snapback primer genotyping of the Gilbert syndrome UGT1A1 (TA)(n) promoter polymorphism by high-resolution melting.

Farrar JS, Palais RA, Wittwer CT.

Clin Chem. 2011 Sep;57(9):1303-10. doi: 10.1373/clinchem.2011.166306. Epub 2011 Jul 19.


Primer sequence disclosure: a clarification of the MIQE guidelines.

Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT.

Clin Chem. 2011 Jun;57(6):919-21. doi: 10.1373/clinchem.2011.162958. Epub 2011 Mar 18. No abstract available.


uMELT: prediction of high-resolution melting curves and dynamic melting profiles of PCR products in a rich web application.

Dwight Z, Palais R, Wittwer CT.

Bioinformatics. 2011 Apr 1;27(7):1019-20. doi: 10.1093/bioinformatics/btr065. Epub 2011 Feb 7.


Detecting rare thrombophilia variants by high-resolution melting.

Seipp MT, Wittwer CT.

Clin Chem. 2011 Apr;57(4):639-41. doi: 10.1373/clinchem.2010.159160. Epub 2011 Feb 3. No abstract available.


Making DNA melting useful.

Wittwer CT.

Clin Chem. 2010 Sep;56(9):1500-1. doi: 10.1373/clinchem.2010.146175. Epub 2010 Jun 25. No abstract available.


Guidelines for the diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria and related disorders by flow cytometry.

Borowitz MJ, Craig FE, Digiuseppe JA, Illingworth AJ, Rosse W, Sutherland DR, Wittwer CT, Richards SJ; Clinical Cytometry Society.

Cytometry B Clin Cytom. 2010 Jul;78(4):211-30. doi: 10.1002/cyto.b.20525.


Enrichment and detection of rare alleles by means of snapback primers and rapid-cycle PCR.

Zhou L, Palais RA, Smith GD, Anderson D, Rowe LR, Wittwer CT.

Clin Chem. 2010 May;56(5):814-22. doi: 10.1373/clinchem.2009.142034. Epub 2010 Mar 18.


Rapid genetic analysis of x-linked chronic granulomatous disease by high-resolution melting.

Hill HR, Augustine NH, Pryor RJ, Reed GH, Bagnato JD, Tebo AE, Bender JM, Pasi BM, Chinen J, Hanson IC, de Boer M, Roos D, Wittwer CT.

J Mol Diagn. 2010 May;12(3):368-76. doi: 10.2353/jmoldx.2010.090147. Epub 2010 Mar 12.


High-resolution DNA melting analysis in clinical research and diagnostics.

Montgomery JL, Sanford LN, Wittwer CT.

Expert Rev Mol Diagn. 2010 Mar;10(2):219-40. doi: 10.1586/erm.09.84. Review.


Rapid molecular analysis of the STAT3 gene in Job syndrome of hyper-IgE and recurrent infectious diseases.

Kumánovics A, Wittwer CT, Pryor RJ, Augustine NH, Leppert MF, Carey JC, Ochs HD, Wedgwood RJ, Faville RJ Jr, Quie PG, Hill HR.

J Mol Diagn. 2010 Mar;12(2):213-9. doi: 10.2353/jmoldx.2010.090080. Epub 2010 Jan 21.


High resolution melting analysis for gene scanning.

Erali M, Wittwer CT.

Methods. 2010 Apr;50(4):250-61. doi: 10.1016/j.ymeth.2010.01.013. Epub 2010 Jan 18.

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