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Items: 1 to 50 of 223

1.

Inositol Pyrophosphate InsP8 Acts as an Intracellular Phosphate Signal in Arabidopsis.

Dong J, Ma G, Sui L, Wei M, Satheesh V, Zhang R, Ge S, Li J, Zhang TE, Wittwer C, Jessen HJ, Zhang H, An GY, Chao DY, Liu D, Lei M.

Mol Plant. 2019 Aug 13. pii: S1674-2052(19)30263-1. doi: 10.1016/j.molp.2019.08.002. [Epub ahead of print]

PMID:
31419530
2.

The kinetic requirements of extreme qPCR.

Millington AL, Houskeeper JA, Quackenbush JF, Trauba JM, Wittwer CT.

Biomol Detect Quantif. 2019 Mar 13;17:100081. doi: 10.1016/j.bdq.2019.100081. eCollection 2019 Mar.

3.

Evaluation of Mass Cytometry in the Clinical Laboratory.

Ravkov EV, Charlton CM, Barker AP, Hill H, Peterson LK, Slev P, Tebo A, Voelkerding KV, Wittwer CT, Heikal N, Delgado JC, Lázár-Molnár E, Kumánovics A.

Cytometry B Clin Cytom. 2019 Jul;96(4):266-274. doi: 10.1002/cyto.b.21791. Epub 2019 Jun 7.

PMID:
31173473
4.

Photolysis of cell-permeant caged inositol pyrophosphates controls oscillations of cytosolic calcium in a β-cell line.

Hauke S, Dutta AK, Eisenbeis VB, Bezold D, Bittner T, Wittwer C, Thakor D, Pavlovic I, Schultz C, Jessen HJ.

Chem Sci. 2019 Jan 10;10(9):2687-2692. doi: 10.1039/c8sc03479f. eCollection 2019 Mar 7.

5.

Integrated Extreme Real-Time PCR and High-Speed Melting Analysis in 52 to 87 Seconds.

Myrick JT, Pryor RJ, Palais RA, Ison SJ, Sanford L, Dwight ZL, Huuskonen JJ, Sundberg SO, Wittwer CT.

Clin Chem. 2019 Feb;65(2):263-271. doi: 10.1373/clinchem.2018.296608. Epub 2018 Nov 20.

PMID:
30459167
6.

5-Diphosphoinositol pentakisphosphate (5-IP7) regulates phosphate release from acidocalcisomes and yeast vacuoles.

Potapenko E, Cordeiro CD, Huang G, Storey M, Wittwer C, Dutta AK, Jessen HJ, Starai VJ, Docampo R.

J Biol Chem. 2018 Dec 7;293(49):19101-19112. doi: 10.1074/jbc.RA118.005884. Epub 2018 Oct 12.

PMID:
30315104
7.

Hydrophilic interaction liquid chromatography-tandem mass spectrometry for the quantitative analysis of mammalian-derived inositol poly/pyrophosphates.

Ito M, Fujii N, Wittwer C, Sasaki A, Tanaka M, Bittner T, Jessen HJ, Saiardi A, Takizawa S, Nagata E.

J Chromatogr A. 2018 Oct 26;1573:87-97. doi: 10.1016/j.chroma.2018.08.061. Epub 2018 Aug 31.

PMID:
30220429
8.

Accuracy, limitations and cost efficiency of eDNA-based community survey in tropical frogs.

Bálint M, Nowak C, Márton O, Pauls SU, Wittwer C, Aramayo JL, Schulze A, Chambert T, Cocchiararo B, Jansen M.

Mol Ecol Resour. 2018 Nov;18(6):1415-1426. doi: 10.1111/1755-0998.12934. Epub 2018 Aug 29.

PMID:
30155977
9.

A "Culture" Shift: Broad Bacterial Detection, Identification, and Antimicrobial Susceptibility Testing Directly from Whole Blood.

Andini N, Hu A, Zhou L, Cogill S, Wang TH, Wittwer CT, Yang S.

Clin Chem. 2018 Oct;64(10):1453-1462. doi: 10.1373/clinchem.2018.290189. Epub 2018 Aug 7.

10.

An approach to analytical validation and testing of body fluid assays for the automated clinical laboratory.

Block DR, Ouverson LJ, Wittwer CA, Saenger AK, Baumann NA.

Clin Biochem. 2018 Aug;58:44-52. doi: 10.1016/j.clinbiochem.2018.05.002. Epub 2018 May 5.

PMID:
29738695
11.

The Effect of Single Mismatches on Primer Extension.

Rejali NA, Moric E, Wittwer CT.

Clin Chem. 2018 May;64(5):801-809. doi: 10.1373/clinchem.2017.282285. Epub 2018 Feb 14.

12.

Quantifying variant differences in DNA melting curves: Effects of length, melting rate, and curve overlay.

Li M, Palais RA, Zhou L, Wittwer CT.

Anal Biochem. 2017 Dec 15;539:90-95. doi: 10.1016/j.ab.2017.10.015. Epub 2017 Oct 21.

PMID:
29061329
13.

High-Speed Melting Analysis: The Effect of Melting Rate on Small Amplicon Microfluidic Genotyping.

Pryor RJ, Myrick JT, Palais RA, Sundberg SO, Paek JY, Wittwer CT, Knight IT.

Clin Chem. 2017 Oct;63(10):1624-1632. doi: 10.1373/clinchem.2017.276147. Epub 2017 Aug 17.

14.

MIQE: A Step Toward More Robust and Reproducible Quantitative PCR.

Bustin SA, Wittwer CT.

Clin Chem. 2017 Sep;63(9):1537-1538. doi: 10.1373/clinchem.2016.268953. Epub 2017 Jun 12. Review. No abstract available.

15.

Anti-ACSA-2 defines a novel monoclonal antibody for prospective isolation of living neonatal and adult astrocytes.

Kantzer CG, Boutin C, Herzig ID, Wittwer C, Reiß S, Tiveron MC, Drewes J, Rockel TD, Ohlig S, Ninkovic J, Cremer H, Pennartz S, Jungblut M, Bosio A.

Glia. 2017 Jun;65(6):990-1004. doi: 10.1002/glia.23140. Epub 2017 Mar 20.

PMID:
28317180
16.

Gain-of-function EGLN1 prolyl hydroxylase (PHD2 D4E:C127S) in combination with EPAS1 (HIF-2α) polymorphism lowers hemoglobin concentration in Tibetan highlanders.

Tashi T, Scott Reading N, Wuren T, Zhang X, Moore LG, Hu H, Tang F, Shestakova A, Lorenzo F, Burjanivova T, Koul P, Guchhait P, Wittwer CT, Julian CG, Shah B, Huff CD, Gordeuk VR, Prchal JT, Ge R.

J Mol Med (Berl). 2017 Jun;95(6):665-670. doi: 10.1007/s00109-017-1519-3. Epub 2017 Feb 23.

PMID:
28233034
17.

Democratizing the Real-Time PCR.

Wittwer CT.

Clin Chem. 2017 Apr;63(4):924-925. doi: 10.1373/clinchem.2016.263269. Epub 2017 Feb 3. No abstract available.

18.

Portable Nanopore Sequencing for Viral Surveillance.

Wittwer CT.

Clin Chem. 2016 Nov;62(11):1427-1429. Epub 2016 Sep 16. No abstract available.

19.

Genomic Copy Number Analysis of HER2-Equivocal Breast Cancers.

Geiersbach KB, Willmore-Payne C, Pasi AV, Paxton CN, Werner TL, Xu X, Wittwer CT, Gulbahce HE, Downs-Kelly E.

Am J Clin Pathol. 2016 Oct;146(4):439-47. doi: 10.1093/ajcp/aqw130. Epub 2016 Sep 10.

PMID:
27614666
20.

Disruptive Innovation in Laboratory Medicine.

Rifai N, Topol E, Chan E, Lo YM, Wittwer CT.

Clin Chem. 2015 Sep;61(9):1129-32. doi: 10.1373/clinchem.2015.243667. Epub 2015 Jul 7. No abstract available.

21.

A dark-brown serum sample.

Willrich MA, Wittwer CA, Baumann NA, Block DR.

Clin Chem. 2015 Jul;61(7):999-1000. doi: 10.1373/clinchem.2015.238493. No abstract available.

22.

Detection of chromosomal translocations in formalin-fixed paraffin-embedded (FFPE) leukemic specimens by digital expression profiling.

Liew M, Mao R, Wittwer CT, Salama ME.

Int J Lab Hematol. 2015 Oct;37(5):690-8. doi: 10.1111/ijlh.12388. Epub 2015 Jun 3.

PMID:
26037935
23.

Dynamic filopodia are required for chemokine-dependent intracellular polarization during guided cell migration in vivo.

Meyen D, Tarbashevich K, Banisch TU, Wittwer C, Reichman-Fried M, Maugis B, Grimaldi C, Messerschmidt EM, Raz E.

Elife. 2015 Apr 15;4. doi: 10.7554/eLife.05279.

24.

Copy number assessment by competitive PCR with limiting deoxynucleotide triphosphates and high-resolution melting.

Zhou L, Palais RA, Paxton CN, Geiersbach KB, Wittwer CT.

Clin Chem. 2015 May;61(5):724-33. doi: 10.1373/clinchem.2014.236208. Epub 2015 Mar 10.

25.

Rare event counting of CD59- red cells in human blood: A 47-month experience using PNH consensus guidelines for WBC and RBC testing in a reference lab.

Liew M, Farley M, Andreasen J, Parker CJ, Wittwer CT.

Cytometry B Clin Cytom. 2015 Jul-Aug;88(4):261-9. doi: 10.1002/cyto.b.21225. Epub 2015 Feb 3.

26.

Molecular diagnostics: a revolution in progress.

Chiu RW, Lo YM, Wittwer CT.

Clin Chem. 2015 Jan;61(1):1-3. doi: 10.1373/clinchem.2014.233361. No abstract available.

27.

Extreme PCR: efficient and specific DNA amplification in 15-60 seconds.

Farrar JS, Wittwer CT.

Clin Chem. 2015 Jan;61(1):145-53. doi: 10.1373/clinchem.2014.228304. Epub 2014 Oct 15.

28.

Microfluidic genotyping by rapid serial PCR and high-speed melting analysis.

Sundberg SO, Wittwer CT, Howell RM, Huuskonen J, Pryor RJ, Farrar JS, Stiles HM, Palais RA, Knight IT.

Clin Chem. 2014 Oct;60(10):1306-13. doi: 10.1373/clinchem.2014.223768. Epub 2014 Aug 12.

29.

Quasi-digital PCR: Enrichment and quantification of rare DNA variants.

Sundberg SO, Wittwer CT, Zhou L, Palais R, Dwight Z, Gale BK.

Biomed Microdevices. 2014 Aug;16(4):639-44. doi: 10.1007/s10544-014-9866-0.

PMID:
24781884
30.

Genotyping accuracy of high-resolution DNA melting instruments.

Li M, Zhou L, Palais RA, Wittwer CT.

Clin Chem. 2014 Jun;60(6):864-72. doi: 10.1373/clinchem.2013.220160. Epub 2014 Apr 9.

31.

The influence of nucleotide sequence and temperature on the activity of thermostable DNA polymerases.

Montgomery JL, Rejali N, Wittwer CT.

J Mol Diagn. 2014 May;16(3):305-13. doi: 10.1016/j.jmoldx.2014.01.006. Epub 2014 Mar 6.

PMID:
24607271
32.

Heterozygote PCR product melting curve prediction.

Dwight ZL, Palais R, Kent J, Wittwer CT.

Hum Mutat. 2014 Mar;35(3):278-82. doi: 10.1002/humu.22494. Epub 2014 Jan 3.

PMID:
24375593
33.

Fluorescence-based temperature control for polymerase chain reaction.

Sanford LN, Wittwer CT.

Anal Biochem. 2014 Mar 1;448:75-81. doi: 10.1016/j.ab.2013.11.027. Epub 2013 Nov 28.

PMID:
24291705
34.

The need for transparency and good practices in the qPCR literature.

Bustin SA, Benes V, Garson J, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley G, Wittwer CT, Schjerling P, Day PJ, Abreu M, Aguado B, Beaulieu JF, Beckers A, Bogaert S, Browne JA, Carrasco-Ramiro F, Ceelen L, Ciborowski K, Cornillie P, Coulon S, Cuypers A, De Brouwer S, De Ceuninck L, De Craene J, De Naeyer H, De Spiegelaere W, Deckers K, Dheedene A, Durinck K, Ferreira-Teixeira M, Fieuw A, Gallup JM, Gonzalo-Flores S, Goossens K, Heindryckx F, Herring E, Hoenicka H, Icardi L, Jaggi R, Javad F, Karampelias M, Kibenge F, Kibenge M, Kumps C, Lambertz I, Lammens T, Markey A, Messiaen P, Mets E, Morais S, Mudarra-Rubio A, Nakiwala J, Nelis H, Olsvik PA, Pérez-Novo C, Plusquin M, Remans T, Rihani A, Rodrigues-Santos P, Rondou P, Sanders R, Schmidt-Bleek K, Skovgaard K, Smeets K, Tabera L, Toegel S, Van Acker T, Van den Broeck W, Van der Meulen J, Van Gele M, Van Peer G, Van Poucke M, Van Roy N, Vergult S, Wauman J, Tshuikina-Wiklander M, Willems E, Zaccara S, Zeka F, Vandesompele J.

Nat Methods. 2013 Nov;10(11):1063-7. doi: 10.1038/nmeth.2697.

PMID:
24173381
35.

Influence of PCR reagents on DNA polymerase extension rates measured on real-time PCR instruments.

Montgomery JL, Wittwer CT.

Clin Chem. 2014 Feb;60(2):334-40. doi: 10.1373/clinchem.2013.212829. Epub 2013 Oct 15.

36.

Quantum method for fluorescence background removal in DNA melting analysis.

Sanford LN, Kent JO, Wittwer CT.

Anal Chem. 2013 Oct 15;85(20):9907-15. doi: 10.1021/ac4024928. Epub 2013 Sep 26.

PMID:
24070125
37.

Continuous fluorescence monitoring of rapid cycle DNA amplification. 1997.

Wittwer CT, Herrmann MG, Moss AA, Rasmussen RP.

Biotechniques. 2013 Jun;54(6):314-20. No abstract available.

38.

Stopped-flow DNA polymerase assay by continuous monitoring of dNTP incorporation by fluorescence.

Montgomery JL, Rejali N, Wittwer CT.

Anal Biochem. 2013 Oct 15;441(2):133-9. doi: 10.1016/j.ab.2013.07.008. Epub 2013 Jul 16.

PMID:
23872003
39.

Circulating nucleosomes and immunogenic cell death markers HMGB1, sRAGE and DNAse in patients with advanced pancreatic cancer undergoing chemotherapy.

Wittwer C, Boeck S, Heinemann V, Haas M, Stieber P, Nagel D, Holdenrieder S.

Int J Cancer. 2013 Dec 1;133(11):2619-30. doi: 10.1002/ijc.28294. Epub 2013 Aug 9.

40.

High resolution melting analysis of the MMAB gene in cblB patients and in those with undiagnosed methylmalonic aciduria.

Illson ML, Dempsey-Nunez L, Kent J, Huang Q, Brebner A, Raff ML, Watkins D, Gilfix BM, Wittwer CT, Rosenblatt DS.

Mol Genet Metab. 2013 Sep-Oct;110(1-2):86-9. doi: 10.1016/j.ymgme.2013.04.020. Epub 2013 May 4.

PMID:
23707710
41.

Cytokeratin 19-fragments (CYFRA 21-1) as a novel serum biomarker for response and survival in patients with advanced pancreatic cancer.

Boeck S, Wittwer C, Heinemann V, Haas M, Kern C, Stieber P, Nagel D, Holdenrieder S.

Br J Cancer. 2013 Apr 30;108(8):1684-94. doi: 10.1038/bjc.2013.158. Epub 2013 Apr 11.

42.

The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments.

Huggett JF, Foy CA, Benes V, Emslie K, Garson JA, Haynes R, Hellemans J, Kubista M, Mueller RD, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT, Bustin SA.

Clin Chem. 2013 Jun;59(6):892-902. doi: 10.1373/clinchem.2013.206375. Epub 2013 Apr 9.

43.

Symmetric snapback primers for scanning and genotyping of the cystic fibrosis transmembrane conductance regulator gene.

Zhou L, Palais RA, Ye F, Chen J, Montgomery JL, Wittwer CT.

Clin Chem. 2013 Jul;59(7):1052-61. doi: 10.1373/clinchem.2013.202689. Epub 2013 Mar 15.

44.

Soluble receptor of advanced glycation end products (sRAGE) indicates response to chemotherapy in pancreatic cancer patients.

Wittwer C, Boeck S, Heinemann V, Haas M, Stieber P, Nagel D, Holdenrieder S.

Int J Clin Pharmacol Ther. 2013 Jan;51(1):67-9. No abstract available.

45.

Monitoring temperature with fluorescence during real-time PCR and melting analysis.

Sanford LN, Wittwer CT.

Anal Biochem. 2013 Mar 1;434(1):26-33. doi: 10.1016/j.ab.2012.10.037. Epub 2012 Nov 7.

PMID:
23142429
46.

High resolution melting analysis of the MMAA gene in patients with cblA and in those with undiagnosed methylmalonic aciduria.

Dempsey-Nunez L, Illson ML, Kent J, Huang Q, Brebner A, Watkins D, Gilfix BM, Wittwer CT, Rosenblatt DS.

Mol Genet Metab. 2012 Nov;107(3):363-7. doi: 10.1016/j.ymgme.2012.09.012. Epub 2012 Sep 15.

PMID:
23026888
47.

uAnalyze: web-based high-resolution DNA melting analysis with comparison to thermodynamic predictions.

Dwight ZL, Palais R, Wittwer CT.

IEEE/ACM Trans Comput Biol Bioinform. 2012 Nov-Dec;9(6):1805-11.

PMID:
22889837
48.

Circulating nucleosomes and biomarkers of immunogenic cell death as predictive and prognostic markers in cancer patients undergoing cytotoxic therapy.

Stoetzer OJ, Wittwer C, Lehner J, Fahmueller YN, Kohles N, Fersching DM, Leszinski G, Roessner J, Holdenrieder S.

Expert Opin Biol Ther. 2012 Jun;12 Suppl 1:S217-24. doi: 10.1517/14712598.2012.689280. Review.

PMID:
22620489
49.

Methodological and preanalytical evaluation of a RAGE immunoassay.

Wittwer C, Lehner J, Fersching D, Siegele B, Stoetzer OJ, Holdenrieder S.

Anticancer Res. 2012 May;32(5):2075-8.

PMID:
22593491
50.

Methodological and preanalytical evaluation of an HMGB1 immunoassay.

Lehner J, Wittwer C, Fersching D, Siegele B, Holdenrieder S, Stoetzer OJ.

Anticancer Res. 2012 May;32(5):2059-62.

PMID:
22593488

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