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Items: 1 to 50 of 436

1.

Directed evolution of an α1,3-fucosyltransferase using a single-cell ultrahigh-throughput screening method.

Tan Y, Zhang Y, Han Y, Liu H, Chen H, Ma F, Withers SG, Feng Y, Yang G.

Sci Adv. 2019 Oct 9;5(10):eaaw8451. doi: 10.1126/sciadv.aaw8451. eCollection 2019 Oct.

2.

Prospecting for microbial α-N-acetylgalactosaminidases yields a new class of GH31 O-glycanase.

Rahfeld P, Wardman JF, Mehr K, Huff D, Morgan-Lang C, Chen HM, Hallam SJ, Withers SG.

J Biol Chem. 2019 Nov 1;294(44):16400-16415. doi: 10.1074/jbc.RA119.010628. Epub 2019 Sep 17.

PMID:
31530641
3.

Passaging of an influenza A(H1N1)pdm09 virus in a difluoro sialic acid inhibitor selects for a novel, but unfit I106M neuraminidase mutant.

McKimm-Breschkin JL, Barrett S, McKenzie-Kludas C, McAuley J, Streltsov VA, Withers SG.

Antiviral Res. 2019 Sep;169:104542. doi: 10.1016/j.antiviral.2019.104542. Epub 2019 Jun 22.

PMID:
31233807
4.

An enzymatic pathway in the human gut microbiome that converts A to universal O type blood.

Rahfeld P, Sim L, Moon H, Constantinescu I, Morgan-Lang C, Hallam SJ, Kizhakkedathu JN, Withers SG.

Nat Microbiol. 2019 Sep;4(9):1475-1485. doi: 10.1038/s41564-019-0469-7. Epub 2019 Jun 10.

PMID:
31182795
5.

Synthesis of modified 1,5-imino-d-xylitols as ligands for lysosomal β-glucocerebrosidase.

Zoidl M, Wolfsgruber A, Schalli M, Nasseri SA, Weber P, Stütz AE, Withers SG, Wrodnigg TM.

Monatsh Chem. 2019;150(5):831-842. doi: 10.1007/s00706-019-02427-1. Epub 2019 May 11.

6.

High-Throughput Recovery and Characterization of Metagenome-Derived Glycoside Hydrolase-Containing Clones as a Resource for Biocatalyst Development.

Armstrong Z, Liu F, Kheirandish S, Chen HM, Mewis K, Duo T, Morgan-Lang C, Hallam SJ, Withers SG.

mSystems. 2019 Jun 4;4(4). pii: e00082-19. doi: 10.1128/mSystems.00082-19.

7.

Development and Application of a High-Throughput Functional Metagenomic Screen for Glycoside Phosphorylases.

Macdonald SS, Armstrong Z, Morgan-Lang C, Osowiecka M, Robinson K, Hallam SJ, Withers SG.

Cell Chem Biol. 2019 Jul 18;26(7):1001-1012.e5. doi: 10.1016/j.chembiol.2019.03.017. Epub 2019 May 9.

PMID:
31080075
8.

Probing the role of an invariant active site His in family GH1 β-glycosidases.

Strazzulli A, Perugino G, Mazzone M, Rossi M, Withers SG, Moracci M.

J Enzyme Inhib Med Chem. 2019 Dec;34(1):973-980. doi: 10.1080/14756366.2019.1608198.

9.

Directed evolution of bacterial polysialyltransferases.

Janesch B, Baumann L, Mark A, Thompson N, Rahmani S, Sim L, Withers SG, Wakarchuk WW.

Glycobiology. 2019 Jul 1;29(7):588-598. doi: 10.1093/glycob/cwz021.

PMID:
30976781
10.

High-Throughput "FP-Tag" Assay for the Identification of Glycosyltransferase Inhibitors.

Gao Z, Ovchinnikova OG, Huang BS, Liu F, Williams DE, Andersen RJ, Lowary TL, Whitfield C, Withers SG.

J Am Chem Soc. 2019 Feb 13;141(6):2201-2204. doi: 10.1021/jacs.8b10940. Epub 2019 Feb 1.

PMID:
30698425
11.

Facile Formation of β-thioGlcNAc Linkages to Thiol-Containing Sugars, Peptides, and Proteins using a Mutant GH20 Hexosaminidase.

Tegl G, Hanson J, Chen HM, Kwan DH, Santana AG, Withers SG.

Angew Chem Int Ed Engl. 2019 Feb 4;58(6):1632-1637. doi: 10.1002/anie.201809928. Epub 2019 Jan 9.

12.

The pKa values of the catalytic residues in the retaining glycoside hydrolase T26H mutant of T4 lysozyme.

Brockerman JA, Okon M, Withers SG, McIntosh LP.

Protein Sci. 2019 Mar;28(3):620-632. doi: 10.1002/pro.3562. Epub 2019 Jan 12.

PMID:
30537432
13.

A Bacterial Expression Platform for Production of Therapeutic Proteins Containing Human-like O-Linked Glycans.

Du T, Buenbrazo N, Kell L, Rahmani S, Sim L, Withers SG, DeFrees S, Wakarchuk W.

Cell Chem Biol. 2019 Feb 21;26(2):203-212.e5. doi: 10.1016/j.chembiol.2018.10.017. Epub 2018 Nov 29.

PMID:
30503285
14.

Endo-fucoidan hydrolases from glycoside hydrolase family 107 (GH107) display structural and mechanistic similarities to α-l-fucosidases from GH29.

Vickers C, Liu F, Abe K, Salama-Alber O, Jenkins M, Springate CMK, Burke JE, Withers SG, Boraston AB.

J Biol Chem. 2018 Nov 23;293(47):18296-18308. doi: 10.1074/jbc.RA118.005134. Epub 2018 Oct 3.

PMID:
30282808
15.

Proximity Ligation-Based Fluorogenic Imaging Agents for Neuraminidases.

Gao Z, Thompson AJ, Paulson JC, Withers SG.

Angew Chem Int Ed Engl. 2018 Oct 8;57(41):13538-13541. doi: 10.1002/anie.201808196. Epub 2018 Sep 14.

16.

Identity and role of the non-conserved acid/base catalytic residue in the GH29 fucosidase from the spider Nephilingis cruentata.

Perrella NN, Withers SG, Lopes AR.

Glycobiology. 2018 Dec 1;28(12):925-932. doi: 10.1093/glycob/cwy083.

PMID:
30204861
17.

Structural Dissection of Helianthamide Reveals the Basis of Its Potent Inhibition of Human Pancreatic α-Amylase.

Tysoe C, Withers SG.

Biochemistry. 2018 Sep 18;57(37):5384-5387. doi: 10.1021/acs.biochem.8b00825. Epub 2018 Sep 7.

PMID:
30180544
18.

Insights into Heptosyltransferase I Catalysis and Inhibition through the Structure of Its Ternary Complex.

Blaukopf M, Worrall L, Kosma P, Strynadka NCJ, Withers SG.

Structure. 2018 Oct 2;26(10):1399-1407.e5. doi: 10.1016/j.str.2018.07.001. Epub 2018 Aug 16.

19.

Synthesis of azido-deoxy and amino-deoxy glycosides and glycosyl fluorides for screening of glycosidase libraries and assembly of substituted glycosides.

Chen HM, Withers SG.

Carbohydr Res. 2018 Sep;467:33-44. doi: 10.1016/j.carres.2018.07.007. Epub 2018 Jul 27.

PMID:
30075363
20.

Metagenomics reveals functional synergy and novel polysaccharide utilization loci in the Castor canadensis fecal microbiome.

Armstrong Z, Mewis K, Liu F, Morgan-Lang C, Scofield M, Durno E, Chen HM, Mehr K, Withers SG, Hallam SJ.

ISME J. 2018 Nov;12(11):2757-2769. doi: 10.1038/s41396-018-0215-9. Epub 2018 Jul 16.

21.

A Mechanism-Based Approach to Screening Metagenomic Libraries for Discovery of Unconventional Glycosidases.

Nasseri SA, Betschart L, Opaleva D, Rahfeld P, Withers SG.

Angew Chem Int Ed Engl. 2018 Aug 27;57(35):11359-11364. doi: 10.1002/anie.201806792. Epub 2018 Aug 1.

PMID:
30001477
22.

Discovery of UDP-Glycosyltransferases and BAHD-Acyltransferases Involved in the Biosynthesis of the Antidiabetic Plant Metabolite Montbretin A.

Irmisch S, Jo S, Roach CR, Jancsik S, Man Saint Yuen M, Madilao LL, O'Neil-Johnson M, Williams R, Withers SG, Bohlmann J.

Plant Cell. 2018 Aug;30(8):1864-1886. doi: 10.1105/tpc.18.00406. Epub 2018 Jul 2.

23.

Modulating the Nucleophile of a Glycoside Hydrolase through Site-Specific Incorporation of Fluoroglutamic Acids.

Kötzler MP, Robinson K, Chen HM, Okon M, McIntosh LP, Withers SG.

J Am Chem Soc. 2018 Jul 5;140(26):8268-8276. doi: 10.1021/jacs.8b04235. Epub 2018 Jun 26.

PMID:
29894173
24.

The Molecular Basis of Polysaccharide Sulfatase Activity and a Nomenclature for Catalytic Subsites in this Class of Enzyme.

Hettle AG, Vickers C, Robb CS, Liu F, Withers SG, Hehemann JH, Boraston AB.

Structure. 2018 May 1;26(5):747-758.e4. doi: 10.1016/j.str.2018.03.012. Epub 2018 Apr 19.

25.

Oversized galactosides as a probe for conformational dynamics in LacY.

Smirnova I, Kasho V, Jiang X, Chen HM, Withers SG, Kaback HR.

Proc Natl Acad Sci U S A. 2018 Apr 17;115(16):4146-4151. doi: 10.1073/pnas.1800706115. Epub 2018 Mar 30.

26.

Potent GH20 N-Acetyl-β-d-hexosaminidase Inhibitors: N-Substituted 3-acetamido-4-amino-5-hydroxymethyl-cyclopentanediols.

Weber P, Nasseri SA, Pabst BM, Torvisco A, Müller P, Paschke E, Tschernutter M, Windischhofer W, Withers SG, Wrodnigg TM, Stütz AE.

Molecules. 2018 Mar 20;23(3). pii: E708. doi: 10.3390/molecules23030708.

27.

Structural and mechanistic analysis of a β-glycoside phosphorylase identified by screening a metagenomic library.

Macdonald SS, Patel A, Larmour VLC, Morgan-Lang C, Hallam SJ, Mark BL, Withers SG.

J Biol Chem. 2018 Mar 2;293(9):3451-3467. doi: 10.1074/jbc.RA117.000948. Epub 2018 Jan 9.

28.

Remarkable Reactivity Differences between Glucosides with Identical Leaving Groups.

Duo T, Robinson K, Greig IR, Chen HM, Patrick BO, Withers SG.

J Am Chem Soc. 2017 Nov 8;139(44):15994-15999. doi: 10.1021/jacs.7b09645. Epub 2017 Oct 24.

PMID:
29035043
29.

Characterization of a thermostable endoglucanase from Cellulomonas fimi ATCC484.

Saxena H, Hsu B, de Asis M, Zierke M, Sim L, Withers SG, Wakarchuk W.

Biochem Cell Biol. 2018 Feb;96(1):68-76. doi: 10.1139/bcb-2017-0150. Epub 2017 Oct 5.

PMID:
28982013
30.

Discovery of New Glycosidases From Metagenomic Libraries.

Armstrong Z, Rahfeld P, Withers SG.

Methods Enzymol. 2017;597:3-23. doi: 10.1016/bs.mie.2017.06.001. Epub 2017 Jul 29.

PMID:
28935108
31.

A Conifer UDP-Sugar Dependent Glycosyltransferase Contributes to Acetophenone Metabolism and Defense against Insects.

Mageroy MH, Jancsik S, Man Saint Yuen M, Fischer M, Withers SG, Paetz C, Schneider B, Mackay J, Bohlmann J.

Plant Physiol. 2017 Oct;175(2):641-651. doi: 10.1104/pp.17.00611. Epub 2017 Aug 9.

32.

Structural basis of Notch O-glucosylation and O-xylosylation by mammalian protein-O-glucosyltransferase 1 (POGLUT1).

Li Z, Fischer M, Satkunarajah M, Zhou D, Withers SG, Rini JM.

Nat Commun. 2017 Aug 4;8(1):185. doi: 10.1038/s41467-017-00255-7.

33.

Glycosyl Cations versus Allylic Cations in Spontaneous and Enzymatic Hydrolysis.

Danby PM, Withers SG.

J Am Chem Soc. 2017 Aug 9;139(31):10629-10632. doi: 10.1021/jacs.7b05628. Epub 2017 Jul 26.

PMID:
28737389
34.

X-ray crystallographic structure of a bacterial polysialyltransferase provides insight into the biosynthesis of capsular polysialic acid.

Lizak C, Worrall LJ, Baumann L, Pfleiderer MM, Volkers G, Sun T, Sim L, Wakarchuk W, Withers SG, Strynadka NCJ.

Sci Rep. 2017 Jul 19;7(1):5842. doi: 10.1038/s41598-017-05627-z.

35.

A new type of pharmacological chaperone for GM1-gangliosidosis related human lysosomal β-galactosidase: N-Substituted 5-amino-1-hydroxymethyl-cyclopentanetriols.

Schalli M, Weber P, Tysoe C, Pabst BM, Thonhofer M, Paschke E, Stütz AE, Tschernutter M, Windischhofer W, Withers SG.

Bioorg Med Chem Lett. 2017 Aug 1;27(15):3431-3435. doi: 10.1016/j.bmcl.2017.05.086. Epub 2017 May 30.

PMID:
28600215
36.

Refolding the unfoldable: A systematic approach for renaturation of Bacillus circulans xylanase.

Kötzler MP, McIntosh LP, Withers SG.

Protein Sci. 2017 Aug;26(8):1555-1563. doi: 10.1002/pro.3181. Epub 2017 May 11.

37.

N-Substituted 5-amino-1-hydroxymethyl-cyclopentanetriols: A new family of activity promotors for a GM1-gangliosidosis related human lysosomal β-galactosidase mutant.

Schalli M, Tysoe C, Fischer R, Pabst BM, Thonhofer M, Paschke E, Rappitsch T, Stütz AE, Tschernutter M, Windischhofer W, Withers SG.

Carbohydr Res. 2017 Apr 18;443-444:15-22. doi: 10.1016/j.carres.2017.03.009. Epub 2017 Mar 11.

PMID:
28319682
38.

A Morita-Baylis-Hillman based route to C-5a-chain-extended 4-epi-isofagomine type glycosidase inhibitors.

Lebl R, Thonhofer M, Tysoe C, Pabst BM, Schalli M, Weber P, Paschke E, Stütz AE, Tschernutter M, Windischhofer W, Withers SG.

Carbohydr Res. 2017 Apr 10;442:31-40. doi: 10.1016/j.carres.2017.03.003. Epub 2017 Mar 6.

PMID:
28288345
39.

Rapid Discovery of Potent and Selective Glycosidase-Inhibiting De Novo Peptides.

Jongkees SAK, Caner S, Tysoe C, Brayer GD, Withers SG, Suga H.

Cell Chem Biol. 2017 Mar 16;24(3):381-390. doi: 10.1016/j.chembiol.2017.02.001. Epub 2017 Mar 2.

40.

Characterization of an extradiol dioxygenase involved in the catabolism of lignin-derived biphenyl.

Kuatsjah E, Chen HM, Withers SG, Eltis LD.

FEBS Lett. 2017 Apr;591(7):1001-1009. doi: 10.1002/1873-3468.12611. Epub 2017 Mar 19.

41.

TIEG1 modulates β-catenin sub-cellular localization and enhances Wnt signaling in bone.

Subramaniam M, Cicek M, Pitel KS, Bruinsma ES, Nelson Holte MH, Withers SG, Rajamannan NM, Secreto FJ, Venuprasad K, Hawse JR.

Nucleic Acids Res. 2017 May 19;45(9):5170-5182. doi: 10.1093/nar/gkx118.

42.

Ultrasensitive Fluorogenic Reagents for Neuraminidase Titration.

Gao Z, Niikura M, Withers SG.

Angew Chem Int Ed Engl. 2017 May 22;56(22):6112-6116. doi: 10.1002/anie.201610544. Epub 2017 Feb 13.

PMID:
28191709
43.

Introducing transgalactosylation activity into a family 42 β-galactosidase.

Strazzulli A, Cobucci-Ponzano B, Carillo S, Bedini E, Corsaro MM, Pocsfalvi G, Withers SG, Rossi M, Moracci M.

Glycobiology. 2017 May 1;27(5):425-437. doi: 10.1093/glycob/cwx013.

PMID:
28158578
44.

C-5a-substituted validamine type glycosidase inhibitors.

Schalli M, Wolfsgruber A, Gonzalez Santana A, Tysoe C, Fischer R, Stütz AE, Thonhofer M, Withers SG.

Carbohydr Res. 2017 Feb 22;440-441:1-9. doi: 10.1016/j.carres.2017.01.006. Epub 2017 Jan 13.

PMID:
28135569
45.

Structure and Mechanism of Staphylococcus aureus TarS, the Wall Teichoic Acid β-glycosyltransferase Involved in Methicillin Resistance.

Sobhanifar S, Worrall LJ, King DT, Wasney GA, Baumann L, Gale RT, Nosella M, Brown ED, Withers SG, Strynadka NC.

PLoS Pathog. 2016 Dec 14;12(12):e1006067. doi: 10.1371/journal.ppat.1006067. eCollection 2016 Dec.

46.

Fungal Glycolipid Hydrolase Inhibitors and Their Effect on Cryptococcus neoformans.

Santana AG, Tysoe C, Hu G, Kronstad J, Goddard-Borger ED, Withers SG.

Chembiochem. 2017 Feb 1;18(3):284-290. doi: 10.1002/cbic.201600538. Epub 2017 Jan 3.

PMID:
27905163
47.

Evaluation of the Significance of Starch Surface Binding Sites on Human Pancreatic α-Amylase.

Zhang X, Caner S, Kwan E, Li C, Brayer GD, Withers SG.

Biochemistry. 2016 Nov 1;55(43):6000-6009. Epub 2016 Oct 24.

PMID:
27756128
48.

Glycosynthase mediated synthesis of psychosine.

Goddard-Borger ED, Tysoe C, Withers SG.

Carbohydr Res. 2016 Nov 29;435:97-99. doi: 10.1016/j.carres.2016.09.013. Epub 2016 Sep 22.

PMID:
27721144
49.

Enzymatic fine-tuning for 2-(6-hydroxynaphthyl) β-D-xylopyranoside synthesis catalyzed by the recombinant β-xylosidase BxTW1 from Talaromyces amestolkiae.

Nieto-Domínguez M, Prieto A, Fernández de Toro B, Cañada FJ, Barriuso J, Armstrong Z, Withers SG, de Eugenio LI, Martínez MJ.

Microb Cell Fact. 2016 Oct 4;15(1):171.

50.

A general and efficient strategy for generating the stable enzymes.

Zhang XF, Yang GY, Zhang Y, Xie Y, Withers SG, Feng Y.

Sci Rep. 2016 Sep 26;6:33797. doi: 10.1038/srep33797.

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