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Biochem Soc Trans. 2019 Jul 11. pii: BST20180399. doi: 10.1042/BST20180399. [Epub ahead of print]

Visualizing the inner life of microbes: practices of multi-color single-molecule localization microscopy in microbiology.

Author information

1
Single-Molecule Microbiology Group, Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology and LOEWE Center for Synthetic Microbiology (SYNMIKRO), Marburg, Germany.
2
Single-Molecule Microbiology Group, Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology and LOEWE Center for Synthetic Microbiology (SYNMIKRO), Marburg, Germany ulrike.endesfelder@synmikro.mpi-marburg.mpg.de.

Abstract

In this review, we discuss multi-color single-molecule imaging and tracking strategies for studying microbial cell biology. We first summarize and compare the methods in a detailed literature review of published studies conducted in bacteria and fungi. We then introduce a guideline on which factors and parameters should be evaluated when designing a new experiment, from fluorophore and labeling choices to imaging routines and data analysis. Finally, we give some insight into some of the recent and promising applications and developments of these techniques and discuss the outlook for this field.

KEYWORDS:

localization-based microscopy; microbial cell biology; single-molecule imaging; single-particle tracking

PMID:
31296734
DOI:
10.1042/BST20180399

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