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Xenogeneic modulation of the ClpCP protease of Bacillus subtilis by a phage-encoded adaptor-like protein.

Mulvenna N, Hantke I, Burchell L, Nicod S, Bell D, Turgay K, Wigneshweraraj S.

J Biol Chem. 2019 Jul 30. pii: jbc.RA119.010007. doi: 10.1074/jbc.RA119.010007. [Epub ahead of print]


Xenogeneic Regulation of the Bacterial Transcription Machinery.

Tabib-Salazar A, Mulvenna N, Severinov K, Matthews SJ, Wigneshweraraj S.

J Mol Biol. 2019 Feb 15. pii: S0022-2836(19)30085-3. doi: 10.1016/j.jmb.2019.02.008. [Epub ahead of print] Review.


New insights into the adaptive transcriptional response to nitrogen starvation in Escherichia coli.

Switzer A, Brown DR, Wigneshweraraj S.

Biochem Soc Trans. 2018 Dec 17;46(6):1721-1728. doi: 10.1042/BST20180502. Epub 2018 Dec 4. Review.


Naturally occurring polymorphisms in the virulence regulator Rsp modulate Staphylococcus aureus survival in blood and antibiotic susceptibility.

Krishna A, Holden MTG, Peacock SJ, Edwards AM, Wigneshweraraj S.

Microbiology. 2018 Sep;164(9):1189-1195. doi: 10.1099/mic.0.000695. Epub 2018 Jul 20.


A novel regulatory factor affecting the transcription of methionine biosynthesis genes in Escherichia coli experiencing sustained nitrogen starvation.

Switzer A, Evangelopoulos D, Figueira R, de Carvalho LPS, Brown DR, Wigneshweraraj S.

Microbiology. 2018 Nov;164(11):1457-1470. doi: 10.1099/mic.0.000683. Epub 2018 Jun 29.


T7 phage factor required for managing RpoS in Escherichia coli.

Tabib-Salazar A, Liu B, Barker D, Burchell L, Qimron U, Matthews SJ, Wigneshweraraj S.

Proc Natl Acad Sci U S A. 2018 Jun 5;115(23):E5353-E5362. doi: 10.1073/pnas.1800429115. Epub 2018 May 22.


A Rapid Colorimetric Method to Visualize Protein Interactions.

Liu B, Wang Z, Lan L, Yang Q, Zhang P, Shi L, Lang Y, Tabib-Salazar A, Wigneshweraraj S, Zhang J, Wang Y, Tang Y, Matthews S, Zhang X.

Chemistry. 2018 May 7;24(26):6727-6731. doi: 10.1002/chem.201800401. Epub 2018 Apr 17.


Conformational heterogeneity and bubble dynamics in single bacterial transcription initiation complexes.

Duchi D, Gryte K, Robb NC, Morichaud Z, Sheppard C, Brodolin K, Wigneshweraraj S, Kapanidis AN.

Nucleic Acids Res. 2018 Jan 25;46(2):677-688. doi: 10.1093/nar/gkx1146.


Phenotypic consequences of RNA polymerase dysregulation in Escherichia coli.

Sarkar P, Switzer A, Peters C, Pogliano J, Wigneshweraraj S.

Nucleic Acids Res. 2017 Nov 2;45(19):11131-11143. doi: 10.1093/nar/gkx733.


Exploring the potential of T7 bacteriophage protein Gp2 as a novel inhibitor of mycobacterial RNA polymerase.

du Plessis J, Cloete R, Burchell L, Sarkar P, Warren RM, Christoffels A, Wigneshweraraj S, Sampson SL.

Tuberculosis (Edinb). 2017 Sep;106:82-90. doi: 10.1016/ Epub 2017 Jul 18.


Full shut-off of Escherichia coli RNA-polymerase by T7 phage requires a small phage-encoded DNA-binding protein.

Tabib-Salazar A, Liu B, Shadrin A, Burchell L, Wang Z, Wang Z, Goren MG, Yosef I, Qimron U, Severinov K, Matthews SJ, Wigneshweraraj S.

Nucleic Acids Res. 2017 Jul 27;45(13):7697-7707. doi: 10.1093/nar/gkx370.


Staphylococcus aureus inactivates daptomycin by releasing membrane phospholipids.

Pader V, Hakim S, Painter KL, Wigneshweraraj S, Clarke TB, Edwards AM.

Nat Microbiol. 2016 Oct 24;2:16194. doi: 10.1038/nmicrobiol.2016.194.


The Xp10 Bacteriophage Protein P7 Inhibits Transcription by the Major and Major Variant Forms of the Host RNA Polymerase via a Common Mechanism.

Brown DR, Sheppard CM, Burchell L, Matthews S, Wigneshweraraj S.

J Mol Biol. 2016 Oct 9;428(20):3911-3919. doi: 10.1016/j.jmb.2016.08.004. Epub 2016 Aug 8.


Adaptation to sustained nitrogen starvation by Escherichia coli requires the eukaryote-like serine/threonine kinase YeaG.

Figueira R, Brown DR, Ferreira D, Eldridge MJ, Burchell L, Pan Z, Helaine S, Wigneshweraraj S.

Sci Rep. 2015 Dec 1;5:17524. doi: 10.1038/srep17524.


The Rsb Phosphoregulatory Network Controls Availability of the Primary Sigma Factor in Chlamydia trachomatis and Influences the Kinetics of Growth and Development.

Thompson CC, Griffiths C, Nicod SS, Lowden NM, Wigneshweraraj S, Fisher DJ, McClure MO.

PLoS Pathog. 2015 Aug 27;11(8):e1005125. doi: 10.1371/journal.ppat.1005125. eCollection 2015 Aug. Erratum in: PLoS Pathog. 2015 Nov;11(11):e1005309.


Regulation of virulence gene expression.

Thomas MS, Wigneshweraraj S.

Virulence. 2014;5(8):832-4. doi: 10.1080/21505594.2014.995573. No abstract available.


Systematic mutational analysis of the LytTR DNA binding domain of Staphylococcus aureus virulence gene transcription factor AgrA.

Nicod SS, Weinzierl RO, Burchell L, Escalera-Maurer A, James EH, Wigneshweraraj S.

Nucleic Acids Res. 2014 Nov 10;42(20):12523-36. doi: 10.1093/nar/gku1015. Epub 2014 Oct 28.


What role does the quorum-sensing accessory gene regulator system play during Staphylococcus aureus bacteremia?

Painter KL, Krishna A, Wigneshweraraj S, Edwards AM.

Trends Microbiol. 2014 Dec;22(12):676-85. doi: 10.1016/j.tim.2014.09.002. Epub 2014 Oct 6. Review.


Combinatorial stress responses: direct coupling of two major stress responses in Escherichia coli.

Brown DR, Barton G, Pan Z, Buck M, Wigneshweraraj S.

Microb Cell. 2014 Sep 1;1(9):315-317. doi: 10.15698/mic2014.09.168.


The Agr quorum-sensing system regulates fibronectin binding but not hemolysis in the absence of a functional electron transport chain.

Pader V, James EH, Painter KL, Wigneshweraraj S, Edwards AM.

Infect Immun. 2014 Oct;82(10):4337-47. doi: 10.1128/IAI.02254-14. Epub 2014 Aug 4.


Nitrogen stress response and stringent response are coupled in Escherichia coli.

Brown DR, Barton G, Pan Z, Buck M, Wigneshweraraj S.

Nat Commun. 2014 Jun 20;5:4115. doi: 10.1038/ncomms5115.


The sabotage of the bacterial transcription machinery by a small bacteriophage protein.

Liu B, Shadrin A, Sheppard C, Mekler V, Xu Y, Severinov K, Matthews S, Wigneshweraraj S.

Bacteriophage. 2014 Jan 1;4(1):e28520. Epub 2014 Mar 12.


Domain movements of the enhancer-dependent sigma factor drive DNA delivery into the RNA polymerase active site: insights from single molecule studies.

Sharma A, Leach RN, Gell C, Zhang N, Burrows PC, Shepherd DA, Wigneshweraraj S, Smith DA, Zhang X, Buck M, Stockley PG, Tuma R.

Nucleic Acids Res. 2014 Apr;42(8):5177-90. doi: 10.1093/nar/gku146. Epub 2014 Feb 19.


A bacteriophage transcription regulator inhibits bacterial transcription initiation by σ-factor displacement.

Liu B, Shadrin A, Sheppard C, Mekler V, Xu Y, Severinov K, Matthews S, Wigneshweraraj S.

Nucleic Acids Res. 2014 Apr;42(7):4294-305. doi: 10.1093/nar/gku080. Epub 2014 Jan 30.


Nitrogen and carbon status are integrated at the transcriptional level by the nitrogen regulator NtrC in vivo.

Schumacher J, Behrends V, Pan Z, Brown DR, Heydenreich F, Lewis MR, Bennett MH, Razzaghi B, Komorowski M, Barahona M, Stumpf MP, Wigneshweraraj S, Bundy JG, Buck M.

MBio. 2013 Nov 19;4(6):e00881-13. doi: 10.1128/mBio.00881-13.


Phage T7 Gp2 inhibition of Escherichia coli RNA polymerase involves misappropriation of σ70 domain 1.1.

Bae B, Davis E, Brown D, Campbell EA, Wigneshweraraj S, Darst SA.

Proc Natl Acad Sci U S A. 2013 Dec 3;110(49):19772-7. doi: 10.1073/pnas.1314576110. Epub 2013 Nov 11.


Transcriptional downregulation of agr expression in Staphylococcus aureus during growth in human serum can be overcome by constitutively active mutant forms of the sensor kinase AgrC.

James EH, Edwards AM, Wigneshweraraj S.

FEMS Microbiol Lett. 2013 Dec;349(2):153-62. doi: 10.1111/1574-6968.12309. Epub 2013 Nov 18.


A non-bacterial transcription factor inhibits bacterial transcription by a multipronged mechanism.

Sheppard C, James E, Barton G, Matthews S, Severinov K, Wigneshweraraj S.

RNA Biol. 2013 Apr;10(4):495-501. doi: 10.4161/rna.24283. Epub 2013 Apr 1.


Overexpression of Escherichia coli udk mimics the absence of T7 Gp2 function and thereby abrogates successful infection by T7 phage.

Shadrin A, Sheppard C, Savalia D, Severinov K, Wigneshweraraj S.

Microbiology. 2013 Feb;159(Pt 2):269-74. doi: 10.1099/mic.0.064527-0. Epub 2012 Dec 14.


Key roles of the downstream mobile jaw of Escherichia coli RNA polymerase in transcription initiation.

Drennan A, Kraemer M, Capp M, Gries T, Ruff E, Sheppard C, Wigneshweraraj S, Artsimovitch I, Record MT Jr.

Biochemistry. 2012 Nov 27;51(47):9447-59. doi: 10.1021/bi301260u. Epub 2012 Nov 14.


Substitutions in the Escherichia coli RNA polymerase inhibitor T7 Gp2 that allow inhibition of transcription when the primary interaction interface between Gp2 and RNA polymerase becomes compromised.

Shadrin A, Sheppard C, Severinov K, Matthews S, Wigneshweraraj S.

Microbiology. 2012 Nov;158(Pt 11):2753-64. doi: 10.1099/mic.0.062547-0. Epub 2012 Sep 13.


Opening and closing of the bacterial RNA polymerase clamp.

Chakraborty A, Wang D, Ebright YW, Korlann Y, Kortkhonjia E, Kim T, Chowdhury S, Wigneshweraraj S, Irschik H, Jansen R, Nixon BT, Knight J, Weiss S, Ebright RH.

Science. 2012 Aug 3;337(6094):591-5. doi: 10.1126/science.1218716.


Structural and mechanistic basis for the inhibition of Escherichia coli RNA polymerase by T7 Gp2.

James E, Liu M, Sheppard C, Mekler V, Cámara B, Liu B, Simpson P, Cota E, Severinov K, Matthews S, Wigneshweraraj S.

Mol Cell. 2012 Sep 14;47(5):755-66. doi: 10.1016/j.molcel.2012.06.013. Epub 2012 Jul 19.


Molecular mechanism of transcription inhibition by phage T7 gp2 protein.

Mekler V, Minakhin L, Sheppard C, Wigneshweraraj S, Severinov K.

J Mol Biol. 2011 Nov 11;413(5):1016-27. doi: 10.1016/j.jmb.2011.09.029. Epub 2011 Sep 21.


Reprint of: inhibition of Escherichia coli RNAp by T7 Gp2 protein: role of negatively charged strip of amino acid residues in Gp2.

Sheppard C, Cámara B, Shadrin A, Akulenko N, Liu M, Baldwin G, Severinov K, Cota E, Matthews S, Wigneshweraraj SR.

J Mol Biol. 2011 Oct 7;412(5):832-41. doi: 10.1016/j.jmb.2011.07.064. Epub 2011 Jul 30.


Molecular insights into the control of transcription initiation at the Staphylococcus aureus agr operon.

Reynolds J, Wigneshweraraj S.

J Mol Biol. 2011 Oct 7;412(5):862-81. doi: 10.1016/j.jmb.2011.06.018. Epub 2011 Jun 25.


Activity map of the Escherichia coli RNA polymerase bridge helix.

Jovanovic M, Burrows PC, Bose D, Cámara B, Wiesler S, Zhang X, Wigneshweraraj S, Weinzierl RO, Buck M.

J Biol Chem. 2011 Apr 22;286(16):14469-79. doi: 10.1074/jbc.M110.212902. Epub 2011 Feb 25.


Inhibition of Escherichia coli RNAp by T7 Gp2 protein: role of negatively charged strip of amino acid residues in Gp2.

Sheppard C, Cámara B, Shadrin A, Akulenko N, Liu M, Baldwin G, Severinov K, Cota E, Matthews S, Wigneshweraraj SR.

J Mol Biol. 2011 Apr 15;407(5):623-32. doi: 10.1016/j.jmb.2011.02.013. Epub 2011 Feb 18.


Sensing DNA opening in transcription using quenchable Förster resonance energy transfer.

Cordes T, Santoso Y, Tomescu AI, Gryte K, Hwang LC, Camará B, Wigneshweraraj S, Kapanidis AN.

Biochemistry. 2010 Nov 2;49(43):9171-80. doi: 10.1021/bi101184g.


T7 phage protein Gp2 inhibits the Escherichia coli RNA polymerase by antagonizing stable DNA strand separation near the transcription start site.

Cámara B, Liu M, Reynolds J, Shadrin A, Liu B, Kwok K, Simpson P, Weinzierl R, Severinov K, Cota E, Matthews S, Wigneshweraraj SR.

Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):2247-52. doi: 10.1073/pnas.0907908107. Epub 2010 Jan 19.


The role of the conserved phenylalanine in the sigma54-interacting GAFTGA motif of bacterial enhancer binding proteins.

Zhang N, Joly N, Burrows PC, Jovanovic M, Wigneshweraraj SR, Buck M.

Nucleic Acids Res. 2009 Oct;37(18):5981-92. doi: 10.1093/nar/gkp658. Epub 2009 Aug 19.


Construction and functional analyses of a comprehensive sigma54 site-directed mutant library using alanine-cysteine mutagenesis.

Xiao Y, Wigneshweraraj SR, Weinzierl R, Wang YP, Buck M.

Nucleic Acids Res. 2009 Jul;37(13):4482-97. doi: 10.1093/nar/gkp419. Epub 2009 May 27.


Coupling sigma factor conformation to RNA polymerase reorganisation for DNA melting.

Burrows PC, Joly N, Cannon WV, Cámara BP, Rappas M, Zhang X, Dawes K, Nixon BT, Wigneshweraraj SR, Buck M.

J Mol Biol. 2009 Mar 27;387(2):306-19. doi: 10.1016/j.jmb.2009.01.052. Epub 2009 Jan 31.


Organization of an activator-bound RNA polymerase holoenzyme.

Bose D, Pape T, Burrows PC, Rappas M, Wigneshweraraj SR, Buck M, Zhang X.

Mol Cell. 2008 Nov 7;32(3):337-46. doi: 10.1016/j.molcel.2008.09.015.


Visualizing the organization and reorganization of transcription complexes for gene expression.

Burrows PC, Wigneshweraraj S, Bose D, Joly N, Schumacher J, Rappas M, Pape T, Stockley PG, Zhang X, Buck M.

Biochem Soc Trans. 2008 Aug;36(Pt 4):776-9. doi: 10.1042/BST0360776.


Modus operandi of the bacterial RNA polymerase containing the sigma54 promoter-specificity factor.

Wigneshweraraj S, Bose D, Burrows PC, Joly N, Schumacher J, Rappas M, Pape T, Zhang X, Stockley P, Severinov K, Buck M.

Mol Microbiol. 2008 May;68(3):538-46. doi: 10.1111/j.1365-2958.2008.06181.x. Epub 2008 Mar 4. Review.


Protein-DNA interactions that govern AAA+ activator-dependent bacterial transcription initiation.

Burrows PC, Wigneshweraraj SR, Buck M.

J Mol Biol. 2008 Jan 4;375(1):43-58. Epub 2007 Oct 23.


Coupling nucleotide hydrolysis to transcription activation performance in a bacterial enhancer binding protein.

Joly N, Rappas M, Wigneshweraraj SR, Zhang X, Buck M.

Mol Microbiol. 2007 Nov;66(3):583-95. Epub 2007 Sep 19.


Sensor I threonine of the AAA+ ATPase transcriptional activator PspF is involved in coupling nucleotide triphosphate hydrolysis to the restructuring of sigma 54-RNA polymerase.

Schumacher J, Joly N, Rappas M, Bradley D, Wigneshweraraj SR, Zhang X, Buck M.

J Biol Chem. 2007 Mar 30;282(13):9825-33. Epub 2007 Jan 22.


A role for the conserved GAFTGA motif of AAA+ transcription activators in sensing promoter DNA conformation.

Dago AE, Wigneshweraraj SR, Buck M, Morett E.

J Biol Chem. 2007 Jan 12;282(2):1087-97. Epub 2006 Nov 6.

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