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Items: 48

1.

Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments.

Masson GR, Burke JE, Ahn NG, Anand GS, Borchers C, Brier S, Bou-Assaf GM, Engen JR, Englander SW, Faber J, Garlish R, Griffin PR, Gross ML, Guttman M, Hamuro Y, Heck AJR, Houde D, Iacob RE, Jørgensen TJD, Kaltashov IA, Klinman JP, Konermann L, Man P, Mayne L, Pascal BD, Reichmann D, Skehel M, Snijder J, Strutzenberg TS, Underbakke ES, Wagner C, Wales TE, Walters BT, Weis DD, Wilson DJ, Wintrode PL, Zhang Z, Zheng J, Schriemer DC, Rand KD.

Nat Methods. 2019 Jul;16(7):595-602. doi: 10.1038/s41592-019-0459-y. Epub 2019 Jun 27. Review.

2.

Characterization of Excipient Effects on Reversible Self-Association, Backbone Flexibility, and Solution Properties of an IgG1 Monoclonal Antibody at High Concentrations: Part 1.

Hu Y, Arora J, Joshi SB, Esfandiary R, Middaugh CR, Weis DD, Volkin DB.

J Pharm Sci. 2019 Jun 13. pii: S0022-3549(19)30373-9. doi: 10.1016/j.xphs.2019.06.005. [Epub ahead of print]

PMID:
31201906
3.

Characterization of Excipient Effects on Reversible Self-Association, Backbone Flexibility, and Solution Properties of an IgG1 Monoclonal Antibody at High Concentrations: Part 2.

Hu Y, Toth RT 4th, Joshi SB, Esfandiary R, Middaugh CR, Volkin DB, Weis DD.

J Pharm Sci. 2019 Jun 11. pii: S0022-3549(19)30368-5. doi: 10.1016/j.xphs.2019.06.001. [Epub ahead of print]

PMID:
31195015
4.

Reliable Identification of Significant Differences in Differential Hydrogen Exchange-Mass Spectrometry Measurements Using a Hybrid Significance Testing Approach.

Hageman TS, Weis DD.

Anal Chem. 2019 Jul 2;91(13):8008-8016. doi: 10.1021/acs.analchem.9b01325. Epub 2019 Jun 11.

PMID:
31099554
5.

A Structural Variant Approach for Establishing a Detection Limit in Differential Hydrogen Exchange-Mass Spectrometry Measurements.

Hageman TS, Weis DD.

Anal Chem. 2019 Jul 2;91(13):8017-8024. doi: 10.1021/acs.analchem.9b01326. Epub 2019 Jun 11.

PMID:
31099552
6.

Interlaboratory Comparison of Hydrogen-Deuterium Exchange Mass Spectrometry Measurements of the Fab Fragment of NISTmAb.

Hudgens JW, Gallagher ES, Karageorgos I, Anderson KW, Filliben JJ, Huang RY, Chen G, Bou-Assaf GM, Espada A, Chalmers MJ, Harguindey E, Zhang HM, Walters BT, Zhang J, Venable J, Steckler C, Park I, Brock A, Lu X, Pandey R, Chandramohan A, Anand GS, Nirudodhi SN, Sperry JB, Rouse JC, Carroll JA, Rand KD, Leurs U, Weis DD, Al-Naqshabandi MA, Hageman TS, Deredge D, Wintrode PL, Papanastasiou M, Lambris JD, Li S, Urata S.

Anal Chem. 2019 Jun 4;91(11):7336-7345. doi: 10.1021/acs.analchem.9b01100. Epub 2019 May 14.

PMID:
31045344
7.

A Collection of Single-Domain Antibodies that Crowd Ricin Toxin's Active Site.

Angalakurthi SK, Vance DJ, Rong Y, Nguyen CMT, Rudolph MJ, Volkin D, Middaugh CR, Weis DD, Mantis NJ.

Antibodies (Basel). 2018 Dec;7(4). pii: 45. doi: 10.3390/antib7040045. Epub 2018 Dec 17.

8.

Fine-Specificity Epitope Analysis Identifies Contact Points on Ricin Toxin Recognized by Protective Monoclonal Antibodies.

Van Slyke G, Angalakurthi SK, Toth RT 4th, Vance DJ, Rong Y, Ehrbar D, Shi Y, Middaugh CR, Volkin DB, Weis DD, Mantis NJ.

Immunohorizons. 2018 Sep;2(8):262-273. doi: 10.4049/immunohorizons.1800042.

9.

Rapid Prediction of Deamidation Rates of Proteins to Assess Their Long-Term Stability Using Hydrogen Exchange-Mass Spectrometry.

Gamage CLD, Hageman TS, Weis DD.

J Pharm Sci. 2019 Jun;108(6):1964-1972. doi: 10.1016/j.xphs.2019.01.019. Epub 2019 Jan 23.

PMID:
30684538
10.
11.

Contribution of an unusual CDR2 element of a single domain antibody in ricin toxin binding affinity and neutralizing activity.

Rudolph MJ, Vance DJ, Kelow S, Angalakurthi SK, Nguyen S, Davis SA, Rong Y, Middaugh CR, Weis DD, Dunbrack R Jr, Karanicolas J, Mantis NJ.

Protein Eng Des Sel. 2018 Jul 1;31(7-8):277-287. doi: 10.1093/protein/gzy022.

12.

Impact of Glycosylation on the Local Backbone Flexibility of Well-Defined IgG1-Fc Glycoforms Using Hydrogen Exchange-Mass Spectrometry.

More AS, Toth RT 4th, Okbazghi SZ, Middaugh CR, Joshi SB, Tolbert TJ, Volkin DB, Weis DD.

J Pharm Sci. 2018 Sep;107(9):2315-2324. doi: 10.1016/j.xphs.2018.04.026. Epub 2018 May 8.

13.

Modulating Antibody Structure and Function through Directed Mutations and Chemical Rescue.

Kaiser CE, Rincon Pabon JP, Khowsathit J, Castaldi MP, Kazmirski SL, Weis DD, Zhang AX, Karanicolas J.

ACS Synth Biol. 2018 Apr 20;7(4):1152-1162. doi: 10.1021/acssynbio.8b00124. Epub 2018 Apr 9.

14.

Evaluation of Hydrogen Exchange Mass Spectrometry as a Stability-Indicating Method for Formulation Excipient Screening for an IgG4 Monoclonal Antibody.

Toth RT 4th, Pace SE, Mills BJ, Joshi SB, Esfandiary R, Middaugh CR, Weis DD, Volkin DB.

J Pharm Sci. 2018 Apr;107(4):1009-1019. doi: 10.1016/j.xphs.2017.12.009. Epub 2017 Dec 18.

PMID:
29269271
15.

Correction to Empirical Correction for Differences in Chemical Exchange Rates in Hydrogen Exchange-Mass Spectrometry Measurements.

Toth RT 4th, Mills BJ, Joshi SB, Esfandiary R, Bishop SM, Middaugh CR, Volkin DB, Weis DD.

Anal Chem. 2017 Dec 19;89(24):13673. doi: 10.1021/acs.analchem.7b04690. Epub 2017 Dec 6. No abstract available.

PMID:
29210567
16.

High-Definition Mapping of Four Spatially Distinct Neutralizing Epitope Clusters on RiVax, a Candidate Ricin Toxin Subunit Vaccine.

Toth RT 4th, Angalakurthi SK, Van Slyke G, Vance DJ, Hickey JM, Joshi SB, Middaugh CR, Volkin DB, Weis DD, Mantis NJ.

Clin Vaccine Immunol. 2017 Dec 5;24(12). pii: e00237-17. doi: 10.1128/CVI.00237-17. Print 2017 Dec.

17.

High-Resolution Epitope Positioning of a Large Collection of Neutralizing and Nonneutralizing Single-Domain Antibodies on the Enzymatic and Binding Subunits of Ricin Toxin.

Vance DJ, Tremblay JM, Rong Y, Angalakurthi SK, Volkin DB, Middaugh CR, Weis DD, Shoemaker CB, Mantis NJ.

Clin Vaccine Immunol. 2017 Dec 5;24(12). pii: e00236-17. doi: 10.1128/CVI.00236-17. Print 2017 Dec.

18.

Empirical Correction for Differences in Chemical Exchange Rates in Hydrogen Exchange-Mass Spectrometry Measurements.

Toth RT 4th, Mills BJ, Joshi SB, Esfandiary R, Bishop SM, Middaugh CR, Volkin DB, Weis DD.

Anal Chem. 2017 Sep 5;89(17):8931-8941. doi: 10.1021/acs.analchem.7b01396. Epub 2017 Aug 11. Erratum in: Anal Chem. 2017 Dec 19;89(24):13673.

PMID:
28753295
19.

Using homology modeling to interrogate binding affinity in neutralization of ricin toxin by a family of single domain antibodies.

Bazzoli A, Vance DJ, Rudolph MJ, Rong Y, Angalakurthi SK, Toth RT 4th, Middaugh CR, Volkin DB, Weis DD, Karanicolas J, Mantis NJ.

Proteins. 2017 Nov;85(11):1994-2008. doi: 10.1002/prot.25353. Epub 2017 Aug 4.

20.

Quantifying Protection in Disordered Proteins Using Millisecond Hydrogen Exchange-Mass Spectrometry and Peptic Reference Peptides.

Al-Naqshabandi MA, Weis DD.

Biochemistry. 2017 Aug 8;56(31):4064-4072. doi: 10.1021/acs.biochem.6b01312. Epub 2017 Jul 27.

PMID:
28675294
21.
22.

Correlating the Effects of Antimicrobial Preservatives on Conformational Stability, Aggregation Propensity, and Backbone Flexibility of an IgG1 mAb.

Arora J, Joshi SB, Middaugh CR, Weis DD, Volkin DB.

J Pharm Sci. 2017 Jun;106(6):1508-1518. doi: 10.1016/j.xphs.2017.02.007. Epub 2017 Feb 14.

PMID:
28212986
23.

Automated Strong Cation-Exchange Cleanup To Remove Macromolecular Crowding Agents for Protein Hydrogen Exchange Mass Spectrometry.

Rusinga FI, Weis DD.

Anal Chem. 2017 Jan 17;89(2):1275-1282. doi: 10.1021/acs.analchem.6b04057. Epub 2016 Dec 23.

PMID:
27936623
24.

Charge-mediated Fab-Fc interactions in an IgG1 antibody induce reversible self-association, cluster formation, and elevated viscosity.

Arora J, Hu Y, Esfandiary R, Sathish HA, Bishop SM, Joshi SB, Middaugh CR, Volkin DB, Weis DD.

MAbs. 2016 Nov/Dec;8(8):1561-1574. Epub 2016 Aug 25.

25.

Label-Free, In-Solution Screening of Peptide Libraries for Binding to Protein Targets Using Hydrogen Exchange Mass Spectrometry.

Maaty WS, Weis DD.

J Am Chem Soc. 2016 Feb 3;138(4):1335-43. doi: 10.1021/jacs.5b11742. Epub 2016 Jan 21.

26.

Hydrogen exchange mass spectrometry reveals protein interfaces and distant dynamic coupling effects during the reversible self-association of an IgG1 monoclonal antibody.

Arora J, Hickey JM, Majumdar R, Esfandiary R, Bishop SM, Samra HS, Middaugh CR, Weis DD, Volkin DB.

MAbs. 2015;7(3):525-39. doi: 10.1080/19420862.2015.1029217.

27.

Correlations between changes in conformational dynamics and physical stability in a mutant IgG1 mAb engineered for extended serum half-life.

Majumdar R, Esfandiary R, Bishop SM, Samra HS, Middaugh CR, Volkin DB, Weis DD.

MAbs. 2015;7(1):84-95. doi: 10.4161/19420862.2014.985494.

28.

Mapping residual structure in intrinsically disordered proteins at residue resolution using millisecond hydrogen/deuterium exchange and residue averaging.

Keppel TR, Weis DD.

J Am Soc Mass Spectrom. 2015 Apr;26(4):547-54. doi: 10.1007/s13361-014-1033-6. Epub 2014 Dec 7.

PMID:
25481641
29.

Hydrogen-deuterium exchange mass spectrometry as an emerging analytical tool for stabilization and formulation development of therapeutic monoclonal antibodies.

Majumdar R, Middaugh CR, Weis DD, Volkin DB.

J Pharm Sci. 2015 Feb;104(2):327-45. doi: 10.1002/jps.24224. Epub 2014 Oct 29. Review.

PMID:
25354868
30.

The designability of protein switches by chemical rescue of structure: mechanisms of inactivation and reactivation.

Xia Y, DiPrimio N, Keppel TR, Vo B, Fraser K, Battaile KP, Egan C, Bystroff C, Lovell S, Weis DD, Anderson JC, Karanicolas J.

J Am Chem Soc. 2013 Dec 18;135(50):18840-9. doi: 10.1021/ja407644b. Epub 2013 Dec 6.

31.

High-throughput biophysical analysis and data visualization of conformational stability of an IgG1 monoclonal antibody after deglycosylation.

Alsenaidy MA, Kim JH, Majumdar R, Weis DD, Joshi SB, Tolbert TJ, Middaugh CR, Volkin DB.

J Pharm Sci. 2013 Nov;102(11):3942-56. doi: 10.1002/jps.23730. Epub 2013 Sep 24.

32.

Analysis of overlapped and noisy hydrogen/deuterium exchange mass spectra.

Guttman M, Weis DD, Engen JR, Lee KK.

J Am Soc Mass Spectrom. 2013 Dec;24(12):1906-12. doi: 10.1007/s13361-013-0727-5. Epub 2013 Sep 10.

33.

Partial cooperative unfolding in proteins as observed by hydrogen exchange mass spectrometry.

Engen JR, Wales TE, Chen S, Marzluff EM, Hassell KM, Weis DD, Smithgall TE.

Int Rev Phys Chem. 2013 Jan 1;32(1):96-127.

34.

Correlating excipient effects on conformational and storage stability of an IgG1 monoclonal antibody with local dynamics as measured by hydrogen/deuterium-exchange mass spectrometry.

Manikwar P, Majumdar R, Hickey JM, Thakkar SV, Samra HS, Sathish HA, Bishop SM, Middaugh CR, Weis DD, Volkin DB.

J Pharm Sci. 2013 Jul;102(7):2136-51. doi: 10.1002/jps.23543. Epub 2013 Apr 25.

PMID:
23620222
35.

Effects of salts from the Hofmeister series on the conformational stability, aggregation propensity, and local flexibility of an IgG1 monoclonal antibody.

Majumdar R, Manikwar P, Hickey JM, Samra HS, Sathish HA, Bishop SM, Middaugh CR, Volkin DB, Weis DD.

Biochemistry. 2013 May 14;52(19):3376-89. doi: 10.1021/bi400232p. Epub 2013 May 2.

PMID:
23594236
36.

Analysis of disordered proteins using a simple apparatus for millisecond quench-flow H/D exchange.

Keppel TR, Weis DD.

Anal Chem. 2013 May 21;85(10):5161-8. doi: 10.1021/ac4004979. Epub 2013 May 1.

PMID:
23586525
37.

Minimizing carry-over in an online pepsin digestion system used for the H/D exchange mass spectrometric analysis of an IgG1 monoclonal antibody.

Majumdar R, Manikwar P, Hickey JM, Arora J, Middaugh CR, Volkin DB, Weis DD.

J Am Soc Mass Spectrom. 2012 Dec;23(12):2140-8. doi: 10.1007/s13361-012-0485-9. Epub 2012 Sep 20.

PMID:
22993047
38.

Structural basis for activation of calcineurin by calmodulin.

Rumi-Masante J, Rusinga FI, Lester TE, Dunlap TB, Williams TD, Dunker AK, Weis DD, Creamer TP.

J Mol Biol. 2012 Jan 13;415(2):307-17. doi: 10.1016/j.jmb.2011.11.008. Epub 2011 Nov 12.

39.

An efficient and inexpensive refrigerated LC system for H/D exchange mass spectrometry.

Keppel TR, Jacques ME, Young RW, Ratzlaff KL, Weis DD.

J Am Soc Mass Spectrom. 2011 Aug;22(8):1472-6. doi: 10.1007/s13361-011-0152-6. Epub 2011 May 15.

PMID:
21953202
40.

Mapping unstructured regions and synergistic folding in intrinsically disordered proteins with amide H/D exchange mass spectrometry.

Keppel TR, Howard BA, Weis DD.

Biochemistry. 2011 Oct 11;50(40):8722-32. doi: 10.1021/bi200875p. Epub 2011 Sep 19. Erratum in: Biochemistry. 2012 Oct 2;51(39):7812.

PMID:
21894929
41.

A repulsive electrostatic mechanism for protein export through the type III secretion apparatus.

Rathinavelan T, Zhang L, Picking WL, Weis DD, De Guzman RN, Im W.

Biophys J. 2010 Feb 3;98(3):452-61. doi: 10.1016/j.bpj.2009.10.030.

42.

The use of acetone as a substitute for acetonitrile in analysis of peptides by liquid chromatography/electrospray ionization mass spectrometry.

Keppel TR, Jacques ME, Weis DD.

Rapid Commun Mass Spectrom. 2010 Jan;24(1):6-10. doi: 10.1002/rcm.4352.

PMID:
19957295
43.

Functional characterization and conformational analysis of the Herpesvirus saimiri Tip-C484 protein.

Mitchell JL, Trible RP, Emert-Sedlak LA, Weis DD, Lerner EC, Applen JJ, Sefton BM, Smithgall TE, Engen JR.

J Mol Biol. 2007 Mar 2;366(4):1282-93. Epub 2006 Dec 16.

44.

Altered dynamics in Lck SH3 upon binding to the LBD1 domain of Herpesvirus saimiri Tip.

Weis DD, Kjellen P, Sefton BM, Engen JR.

Protein Sci. 2006 Oct;15(10):2402-10.

45.

Semi-automated data processing of hydrogen exchange mass spectra using HX-Express.

Weis DD, Engen JR, Kass IJ.

J Am Soc Mass Spectrom. 2006 Dec;17(12):1700-3. Epub 2006 Aug 22.

46.

Identification and characterization of EX1 kinetics in H/D exchange mass spectrometry by peak width analysis.

Weis DD, Wales TE, Engen JR, Hotchko M, Ten Eyck LF.

J Am Soc Mass Spectrom. 2006 Nov;17(11):1498-1509. doi: 10.1016/j.jasms.2006.05.014. Epub 2006 Jul 27.

47.
48.

Absorption Anomalies in Ratio and Subtraction FT-IR Spectroscopy.

Weis DD, Ewing GE.

Anal Chem. 1998 Aug 1;70(15):3175-83. doi: 10.1021/ac971174a.

PMID:
21644655

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