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Items: 50

1.

GMO-free RNAi: exogenous application of RNA molecules in plants.

Dalakouras A, Wassenegger M, Dadami E, Ganopoulos I, Pappas M, Papadopoulou KK.

Plant Physiol. 2019 Jul 8. pii: pp.00570.2019. doi: 10.1104/pp.19.00570. [Epub ahead of print]

2.

The Rep proteins encoded by alphasatellites restore expression of a transcriptionally silenced green fluorescent protein transgene in Nicotiana benthamiana.

Abbas Q, Amin I, Mansoor S, Shafiq M, Wassenegger M, Briddon RW.

Virusdisease. 2019 Mar;30(1):101-105. doi: 10.1007/s13337-017-0413-5. Epub 2017 Dec 5.

PMID:
31143837
3.

Transient expression of intron-containing transgenes generates non-spliced aberrant pre-mRNAs that are processed into siRNAs.

Dalakouras A, Lauter A, Bassler A, Krczal G, Wassenegger M.

Planta. 2019 Feb;249(2):457-468. doi: 10.1007/s00425-018-3015-6. Epub 2018 Sep 24.

PMID:
30251012
4.

Delivery of Hairpin RNAs and Small RNAs Into Woody and Herbaceous Plants by Trunk Injection and Petiole Absorption.

Dalakouras A, Jarausch W, Buchholz G, Bassler A, Braun M, Manthey T, Krczal G, Wassenegger M.

Front Plant Sci. 2018 Aug 24;9:1253. doi: 10.3389/fpls.2018.01253. eCollection 2018.

5.

Induction of Silencing in Plants by High-Pressure Spraying of In vitro-Synthesized Small RNAs.

Dalakouras A, Wassenegger M, McMillan JN, Cardoza V, Maegele I, Dadami E, Runne M, Krczal G, Wassenegger M.

Front Plant Sci. 2016 Aug 30;7:1327. doi: 10.3389/fpls.2016.01327. eCollection 2016.

6.

RNA-directed DNA methylation efficiency depends on trigger and target sequence identity.

Dalakouras A, Dadami E, Wassenegger M, Krczal G, Wassenegger M.

Plant J. 2016 Jul;87(2):202-14. doi: 10.1111/tpj.13193. Epub 2016 Jul 26.

7.

Functional Analysis of Cotton Leaf Curl Kokhran Virus/Cotton Leaf Curl Multan Betasatellite RNA Silencing Suppressors.

Saeed M, Briddon RW, Dalakouras A, Krczal G, Wassenegger M.

Biology (Basel). 2015 Oct 23;4(4):697-714. doi: 10.3390/biology4040697.

8.

Replicating Potato spindle tuber viroid mediates de novo methylation of an intronic viroid sequence but no cleavage of the corresponding pre-mRNA.

Dalakouras A, Dadami E, Bassler A, Zwiebel M, Krczal G, Wassenegger M.

RNA Biol. 2015;12(3):268-75. doi: 10.1080/15476286.2015.1017216.

9.

Engineering viroid resistance.

Dalakouras A, Dadami E, Wassenegger M.

Viruses. 2015 Feb 10;7(2):634-46. doi: 10.3390/v7020634. Review.

10.

Three gene products of a begomovirus-betasatellite complex restore expression of a transcriptionally silenced green fluorescent protein transgene in Nicotiana benthamiana.

Saeed M, Krczal G, Wassenegger M.

Virus Genes. 2015 Apr;50(2):340-4. doi: 10.1007/s11262-014-1155-8. Epub 2014 Dec 24.

PMID:
25537949
11.

Viroid-induced DNA methylation in plants.

Dalakouras A, Dadami E, Wassenegger M.

Biomol Concepts. 2013 Dec;4(6):557-65. doi: 10.1515/bmc-2013-0030. Review.

PMID:
25436756
12.

An endogene-resembling transgene is resistant to DNA methylation and systemic silencing.

Dadami E, Dalakouras A, Zwiebel M, Krczal G, Wassenegger M.

RNA Biol. 2014;11(7):934-41. doi: 10.4161/rna.29623. Epub 2014 Jul 23.

13.
14.

Binding and processing of small dsRNA molecules by the class 1 RNase III protein encoded by sweet potato chlorotic stunt virus.

Weinheimer I, Boonrod K, Moser M, Wassenegger M, Krczal G, Butcher SJ, Valkonen JP.

J Gen Virol. 2014 Feb;95(Pt 2):486-95. doi: 10.1099/vir.0.058693-0. Epub 2013 Nov 1.

PMID:
24187016
15.

An endogene-resembling transgene delays the onset of silencing and limits siRNA accumulation.

Dadami E, Moser M, Zwiebel M, Krczal G, Wassenegger M, Dalakouras A.

FEBS Lett. 2013 Mar 18;587(6):706-10. doi: 10.1016/j.febslet.2013.01.045. Epub 2013 Feb 1.

16.

Revisiting RNA-directed DNA methylation.

Dalakouras A, Wassenegger M.

RNA Biol. 2013 Mar;10(3):453-5. doi: 10.4161/rna.23542. Epub 2013 Jan 16.

17.

Transgenerational maintenance of transgene body CG but not CHG and CHH methylation.

Dalakouras A, Dadami E, Zwiebel M, Krczal G, Wassenegger M.

Epigenetics. 2012 Sep;7(9):1071-8. Epub 2012 Aug 6.

18.

Analysis of the autoproteolytic activity of the recombinant helper component proteinase from zucchini yellow mosaic virus.

Boonrod K, Füllgrabe MW, Krczal G, Wassenegger M.

Biol Chem. 2011 Oct;392(10):937-45. doi: 10.1515/BC.2011.097. Epub 2011 Aug 28.

PMID:
21871010
19.

Diverse spontaneous silencing of a transgene among two Nicotiana species.

Dalakouras A, Moser M, Boonrod K, Krczal G, Wassenegger M.

Planta. 2011 Oct;234(4):699-707. doi: 10.1007/s00425-011-1433-9. Epub 2011 May 27.

PMID:
21617990
20.

The helper component-proteinase of the Zucchini yellow mosaic virus inhibits the Hua Enhancer 1 methyltransferase activity in vitro.

Jamous RM, Boonrod K, Fuellgrabe MW, Ali-Shtayeh MS, Krczal G, Wassenegger M.

J Gen Virol. 2011 Sep;92(Pt 9):2222-6. doi: 10.1099/vir.0.031534-0. Epub 2011 May 18.

PMID:
21593273
21.

Expression, purification and functional characterization of recombinant Zucchini yellow mosaic virus HC-Pro.

Fuellgrabe MW, Boonrod K, Jamous R, Moser M, Shiboleth Y, Krczal G, Wassenegger M.

Protein Expr Purif. 2011 Jan;75(1):40-5. doi: 10.1016/j.pep.2010.07.008. Epub 2010 Jul 30.

PMID:
20674747
22.

Hairpin transcription does not necessarily lead to efficient triggering of the RNAi pathway.

Dalakouras A, Tzanopoulou M, Tsagris M, Wassenegger M, Kalantidis K.

Transgenic Res. 2011 Apr;20(2):293-304. doi: 10.1007/s11248-010-9416-3. Epub 2010 Jun 26.

PMID:
20582569
23.

A chimeric satellite transgene sequence is inefficiently targeted by viroid-induced DNA methylation in tobacco.

Dalakouras A, Moser M, Krczal G, Wassenegger M.

Plant Mol Biol. 2010 Jul;73(4-5):439-47. doi: 10.1007/s11103-010-9631-6. Epub 2010 Apr 3.

PMID:
20364297
24.

Analysis of an autoproteolytic activity of rice yellow mottle virus silencing suppressor P1.

Weinheimer I, Boonrod K, Moser M, Zwiebel M, Füllgrabe M, Krczal G, Wassenegger M.

Biol Chem. 2010 Feb-Mar;391(2-3):271-81. doi: 10.1515/BC.2010.022.

PMID:
20030588
25.

A hairpin RNA construct residing in an intron efficiently triggered RNA-directed DNA methylation in tobacco.

Dalakouras A, Moser M, Zwiebel M, Krczal G, Hell R, Wassenegger M.

Plant J. 2009 Dec;60(5):840-51. doi: 10.1111/j.1365-313X.2009.04003.x. Epub 2009 Aug 21.

26.

RNAi-mediated resistance to Potato spindle tuber viroid in transgenic tomato expressing a viroid hairpin RNA construct.

Schwind N, Zwiebel M, Itaya A, Ding B, Wang MB, Krczal G, Wassenegger M.

Mol Plant Pathol. 2009 Jul;10(4):459-69. doi: 10.1111/j.1364-3703.2009.00546.x.

PMID:
19523100
27.

Strategies for antiviral resistance in transgenic plants.

Prins M, Laimer M, Noris E, Schubert J, Wassenegger M, Tepfer M.

Mol Plant Pathol. 2008 Jan;9(1):73-83. doi: 10.1111/j.1364-3703.2007.00447.x. Review.

PMID:
18705886
28.

First Report of Solanum jasminoides Infected by Citrus exocortis viroid in Germany and the Netherlands and Tomato apical stunt viroid in Belgium and Germany.

Verhoeven JTJ, Jansen CCC, Roenhorst JW, Steyer S, Schwind N, Wassenegger M.

Plant Dis. 2008 Jun;92(6):973. doi: 10.1094/PDIS-92-6-0973A.

PMID:
30769734
29.

The conserved FRNK box in HC-Pro, a plant viral suppressor of gene silencing, is required for small RNA binding and mediates symptom development.

Shiboleth YM, Haronsky E, Leibman D, Arazi T, Wassenegger M, Whitham SA, Gaba V, Gal-On A.

J Virol. 2007 Dec;81(23):13135-48. Epub 2007 Sep 26.

30.

Nomenclature and functions of RNA-directed RNA polymerases.

Wassenegger M, Krczal G.

Trends Plant Sci. 2006 Mar;11(3):142-51. Epub 2006 Feb 13. Review.

PMID:
16473542
31.

The role of the RNAi machinery in heterochromatin formation.

Wassenegger M.

Cell. 2005 Jul 15;122(1):13-6. Review.

32.

Direct role of a viroid RNA motif in mediating directional RNA trafficking across a specific cellular boundary.

Qi Y, Pélissier T, Itaya A, Hunt E, Wassenegger M, Ding B.

Plant Cell. 2004 Jul;16(7):1741-52. Epub 2004 Jun 11.

33.

Viroid-induced RNA silencing of GFP-viroid fusion transgenes does not induce extensive spreading of methylation or transitive silencing.

Vogt U, Pélissier T, Pütz A, Razvi F, Fischer R, Wassenegger M.

Plant J. 2004 Apr;38(1):107-18.

34.

Gene silencing-based disease resistance.

Wassenegger M.

Transgenic Res. 2002 Dec;11(6):639-53. Review.

PMID:
12509140
35.

Gene silencing.

Wassenegger M.

Int Rev Cytol. 2002;219:61-113. Review.

PMID:
12211631
36.

Binding of IRE-BP to its cognate RNA sequence: SFM studies on a universal RNA backbone for the analysis of RNA-protein interaction.

Bonin M, Oberstrass J, Vogt U, Wassenegger M, Nellen W.

Biol Chem. 2001 Aug;382(8):1157-62.

PMID:
11592396
37.
38.

RNA-directed DNA methylation.

Wassenegger M.

Plant Mol Biol. 2000 Jun;43(2-3):203-20. Review.

PMID:
10999405
39.

A DNA target of 30 bp is sufficient for RNA-directed DNA methylation.

Pélissier T, Wassenegger M.

RNA. 2000 Jan;6(1):55-65.

40.
41.

Signalling in gene silencing.

Wassenegger M, Pélissier T.

Trends Plant Sci. 1999 Jun;4(6):207-209. No abstract available.

PMID:
10366874
42.

Heavy de novo methylation at symmetrical and non-symmetrical sites is a hallmark of RNA-directed DNA methylation.

Pélissier T, Thalmeir S, Kempe D, Sänger HL, Wassenegger M.

Nucleic Acids Res. 1999 Apr 1;27(7):1625-34.

43.

Isolation of an RNA-directed RNA polymerase-specific cDNA clone from tomato.

Schiebel W, Pélissier T, Riedel L, Thalmeir S, Schiebel R, Kempe D, Lottspeich F, Sänger HL, Wassenegger M.

Plant Cell. 1998 Dec;10(12):2087-101.

44.

Application of PCR to transgenic plants.

Wassenegger M.

Methods Mol Biol. 1998;92:153-64. No abstract available.

PMID:
9664511
45.

A model for RNA-mediated gene silencing in higher plants.

Wassenegger M, Pélissier T.

Plant Mol Biol. 1998 May;37(2):349-62. Review.

PMID:
9617806
46.

A single nucleotide substitution converts potato spindle tuber viroid (PSTVd) from a noninfectious to an infectious RNA for nicotiana tabacum.

Wassenegger M, Spieker RL, Thalmeir S, Gast FU, Riedel L, Sänger HL.

Virology. 1996 Dec 15;226(2):191-7.

47.

Molecular analysis of the gene family of the signal recognition particle (SRP) RNA of tomato.

Riedel L, Volger U, Luckinger R, Pütz A, Sänger HL, Wassenegger M.

Plant Mol Biol. 1996 Apr;31(1):113-25.

PMID:
8704144
48.

Characterization of the signal recognition particle (SRP) RNA population of tomato (Lycopersicon esculentum).

Riedel L, Pütz A, Hauser MT, Luckinger R, Wassenegger M, Sänger HL.

Plant Mol Biol. 1995 Feb;27(4):669-80.

PMID:
7727745
50.

RNA-directed de novo methylation of genomic sequences in plants.

Wassenegger M, Heimes S, Riedel L, Sänger HL.

Cell. 1994 Feb 11;76(3):567-76.

PMID:
8313476

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