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Seroprevalence of rubella virus antibodies among pregnant women in the Center and South-West regions of Cameroon.

Taku NA, Ndze VN, Abernathy E, Hao L, Waku-Kouomou D, Icenogle JP, Wanji S, Akoachere JKT.

PLoS One. 2019 Nov 21;14(11):e0225594. doi: 10.1371/journal.pone.0225594. eCollection 2019.


Neglected Tropical Diseases as a 'litmus test' for Universal Health Coverage? Understanding who is left behind and why in Mass Drug Administration: Lessons from four country contexts.

Dean L, Ozano K, Adekeye O, Dixon R, Fung EG, Gyapong M, Isiyaku S, Kollie K, Kukula V, Lar L, MacPherson E, Makia C, Kouokam Magne E, Nnamdi DB, Mue Nji T, Ntuen U, Oluwole A, Piotrowski H, Siping M, Tchoffo MN, Tchuem Tchuenté LA, Thomson R, Tsey I, Wanji S, Yashiyi J, Zawolo G, Theobald S.

PLoS Negl Trop Dis. 2019 Nov 21;13(11):e0007847. doi: 10.1371/journal.pntd.0007847. eCollection 2019 Nov.


In vitro maintenance of Mansonella perstans microfilariae and its relevance for drug screening.

Njouendou AJ, Kien CA, Esum ME, Ritter M, Chounna Ndongmo WP, Fombad FF, Gandjui NVT, Njiokou F, Enyong P, Pfarr K, Turner J, Layland LE, Hoerauf A, Wanji S.

Exp Parasitol. 2019 Nov;206:107769. doi: 10.1016/j.exppara.2019.107769. Epub 2019 Sep 30.


Depressive Symptoms Amongst People with Podoconiosis and Lower Limb Lymphoedema of Other Cause in Cameroon: A Cross-Sectional Study.

Semrau M, Davey G, Beng AA, Ndongmo WPC, Njouendou AJ, Wanji S, Deribe K.

Trop Med Infect Dis. 2019 Jul 9;4(3). pii: E102. doi: 10.3390/tropicalmed4030102.


In Silico Identification of Novel Biomarkers and Development of New Rapid Diagnostic Tests for the Filarial Parasites Mansonella perstans and Mansonella ozzardi.

Poole CB, Sinha A, Ettwiller L, Apone L, McKay K, Panchapakesa V, Lima NF, Ferreira MU, Wanji S, Carlow CKS.

Sci Rep. 2019 Jul 16;9(1):10275. doi: 10.1038/s41598-019-46550-9.


Projected number of people with onchocerciasis-loiasis co-infection in Africa, 1995 to 2025.

Vinkeles Melchers NVS, Coffeng LE, Boussinesq M, Pedrique B, Pion SDS, Tekle AH, Zouré HGM, Wanji S, Remme JH, Stolk WA.

Clin Infect Dis. 2019 Jul 13. pii: ciz647. doi: 10.1093/cid/ciz647. [Epub ahead of print]


Armillariella Oral Solution Ameliorates Small Intestinal Damage in a Mouse Model of Chemotherapy-Induced Mucositis.

Wenqin D, Yaodong Z, Wanji S, Fengli Z, Li S, Haili J, Ping L, Mei Z.

Nutr Cancer. 2019;71(7):1142-1152. doi: 10.1080/01635581.2019.1599029. Epub 2019 Jun 18.


A combination of metabolic resistance and high frequency of the 1014F kdr mutation is driving pyrethroid resistance in Anopheles coluzzii population from Guinea savanna of Cameroon.

Fadel AN, Ibrahim SS, Tchouakui M, Terence E, Wondji MJ, Tchoupo M, Wanji S, Wondji CS.

Parasit Vectors. 2019 May 27;12(1):263. doi: 10.1186/s13071-019-3523-7.


Discovery of short-course antiwolbachial quinazolines for elimination of filarial worm infections.

Bakowski MA, Shiroodi RK, Liu R, Olejniczak J, Yang B, Gagaring K, Guo H, White PM, Chappell L, Debec A, Landmann F, Dubben B, Lenz F, Struever D, Ehrens A, Frohberger SJ, Sjoberg H, Pionnier N, Murphy E, Archer J, Steven A, Chunda VC, Fombad FF, Chounna PW, Njouendou AJ, Metuge HM, Ndzeshang BL, Gandjui NV, Akumtoh DN, Kwenti TDB, Woods AK, Joseph SB, Hull MV, Xiong W, Kuhen KL, Taylor MJ, Wanji S, Turner JD, Hübner MP, Hoerauf A, Chatterjee AK, Roland J, Tremblay MS, Schultz PG, Sullivan W, Chu XJ, Petrassi HM, McNamara CW.

Sci Transl Med. 2019 May 8;11(491). pii: eaav3523. doi: 10.1126/scitranslmed.aav3523.


Update on the biology and ecology of Culicoides species in the South-West region of Cameroon with implications on the transmission of Mansonella perstans.

Wanji S, Tayong DB, Ebai R, Opoku V, Kien CA, Ndongmo WPC, Njouendou AJ, Ghani RN, Ritter M, Debrah YA, Layland LE, Enyong PA, Hoerauf A.

Parasit Vectors. 2019 Apr 11;12(1):166. doi: 10.1186/s13071-019-3432-9.


Mouse models of Loa loa.

Pionnier NP, Sjoberg H, Chunda VC, Fombad FF, Chounna PW, Njouendou AJ, Metuge HM, Ndzeshang BL, Gandjui NV, Akumtoh DN, Tayong DB, Taylor MJ, Wanji S, Turner JD.

Nat Commun. 2019 Mar 29;10(1):1429. doi: 10.1038/s41467-019-09442-0.


Preclinical development of an oral anti-Wolbachia macrolide drug for the treatment of lymphatic filariasis and onchocerciasis.

Taylor MJ, von Geldern TW, Ford L, Hübner MP, Marsh K, Johnston KL, Sjoberg HT, Specht S, Pionnier N, Tyrer HE, Clare RH, Cook DAN, Murphy E, Steven A, Archer J, Bloemker D, Lenz F, Koschel M, Ehrens A, Metuge HM, Chunda VC, Ndongmo Chounna PW, Njouendou AJ, Fombad FF, Carr R, Morton HE, Aljayyoussi G, Hoerauf A, Wanji S, Kempf DJ, Turner JD, Ward SA.

Sci Transl Med. 2019 Mar 13;11(483). pii: eaau2086. doi: 10.1126/scitranslmed.aau2086.


Mapping of lymphatic filariasis in loiasis areas: A new strategy shows no evidence for Wuchereria bancrofti endemicity in Cameroon.

Wanji S, Esum ME, Njouendou AJ, Mbeng AA, Chounna Ndongmo PW, Abong RA, Fru J, Fombad FF, Nchanji GT, Ngongeh G, Ngandjui NV, Enyong PI, Storey H, Curtis KC, Fischer K, Fauver JR, Lew D, Goss CW, Fischer PU.

PLoS Negl Trop Dis. 2019 Mar 8;13(3):e0007192. doi: 10.1371/journal.pntd.0007192. eCollection 2019 Mar.


Comparative analysis of IgG and IgG subclasses against Plasmodium falciparum MSP-119 in children from five contrasting bioecological zones of Cameroon.

Kwenti TE, Kukwah TA, Kwenti TDB, Nyassa BR, Dilonga MH, Enow-Orock G, Tendongfor N, Anong ND, Wanji S, Njunda LA, Nkuo-Akenji T.

Malar J. 2019 Jan 22;18(1):16. doi: 10.1186/s12936-019-2654-9.


Short-course, oral flubendazole does not mediate significant efficacy against Onchocerca adult male worms or Brugia microfilariae in murine infection models.

Sjoberg HT, Pionnier N, Aljayyoussi G, Metuge HM, Njouendou AJ, Chunda VC, Fombad FF, Tayong DB, Gandjui NVT, Akumtoh DN, Chounna PWN, Ndzeshang BL, Lachaud S, Tekle F, Quirynen L, Engelen M, Baeten B, Steven A, Ward SA, Taylor MJ, Wanji S, Turner JD.

PLoS Negl Trop Dis. 2019 Jan 16;13(1):e0006356. doi: 10.1371/journal.pntd.0006356. eCollection 2019 Jan.


The central adaptor molecule TRIF influences L. sigmodontis worm development.

Wiszniewsky A, Ritter M, Krupp V, Schulz S, Arndts K, Weighardt H, Wanji S, Hoerauf A, Layland LE.

Parasitol Res. 2019 Feb;118(2):539-549. doi: 10.1007/s00436-018-6159-1. Epub 2019 Jan 15.


Effect of flubendazole on developing stages of Loa loa in vitro and in vivo: a new approach for screening filaricidal agents.

Fombad FF, Njouendou AJ, Ndongmo PC, Ritter M, Chunda VC, Metuge HM, Gandjui NVT, Enyong P, Njiokou F, Hoerauf A, Mackenzie CD, Wanji S.

Parasit Vectors. 2019 Jan 8;12(1):14. doi: 10.1186/s13071-018-3282-x.


Identification and characterization of Loa loa antigens responsible for cross-reactivity with rapid diagnostic tests for lymphatic filariasis.

Hertz MI, Nana-Djeunga H, Kamgno J, Jelil Njouendou A, Chawa Chunda V, Wanji S, Rush A, Fischer PU, Weil GJ, Budge PJ.

PLoS Negl Trop Dis. 2018 Nov 16;12(11):e0006963. doi: 10.1371/journal.pntd.0006963. eCollection 2018 Nov.


Implementation research: new imperatives and opportunities in global health.

Theobald S, Brandes N, Gyapong M, El-Saharty S, Proctor E, Diaz T, Wanji S, Elloker S, Raven J, Elsey H, Bharal S, Pelletier D, Peters DH.

Lancet. 2018 Nov 17;392(10160):2214-2228. doi: 10.1016/S0140-6736(18)32205-0. Epub 2018 Oct 9.


Impact of repeated annual community directed treatment with ivermectin on loiasis parasitological indicators in Cameroon: Implications for onchocerciasis and lymphatic filariasis elimination in areas co-endemic with Loa loa in Africa.

Wanji S, Chounna Ndongmo WP, Fombad FF, Kengne-Ouafo JA, Njouendou AJ, Longang Tchounkeu YF, Koudou B, Bockarie M, Fobi G, Roungou JB, Enyong PA.

PLoS Negl Trop Dis. 2018 Sep 18;12(9):e0006750. doi: 10.1371/journal.pntd.0006750. eCollection 2018 Sep.


Bionomics and vectorial role of anophelines in wetlands along the volcanic chain of Cameroon.

Amvongo-Adjia N, Wirsiy EL, Riveron JM, Chounna Ndongmo WP, Enyong PA, Njiokou F, Wondji CS, Wanji S.

Parasit Vectors. 2018 Aug 14;11(1):471. doi: 10.1186/s13071-018-3041-z.


Predicted distribution and burden of podoconiosis in Cameroon.

Deribe K, Cano J, Njouendou AJ, Eyong ME, Beng AA, Giorgi E, Pigott DM, Pullan RL, Noor AM, Enquselassie F, Murray CJL, Hay SI, Newport MJ, Davey G, Wanji S.

BMJ Glob Health. 2018 Jun 22;3(3):e000730. doi: 10.1136/bmjgh-2018-000730. eCollection 2018.


How Can Onchocerciasis Elimination in Africa Be Accelerated? Modeling the Impact of Increased Ivermectin Treatment Frequency and Complementary Vector Control.

Verver S, Walker M, Kim YE, Fobi G, Tekle AH, Zouré HGM, Wanji S, Boakye DA, Kuesel AC, de Vlas SJ, Boussinesq M, Basáñez MG, Stolk WA.

Clin Infect Dis. 2018 Jun 1;66(suppl_4):S267-S274. doi: 10.1093/cid/cix1137.


Study of lymphoedema of non-filarial origin in the northwest region of Cameroon: spatial distribution, profiling of cases and socio-economic aspects of podoconiosis.

Wanji S, Kengne-Ouafo JA, Deribe K, Tembei AM, Njouendou AJ, Tayong DB, Sofeu-Feugaing DD, Datchoua-Poutcheu FR, Cano J, Giorgi E, Longang-Tchounkeu YF, Enyong PA, Newport MJ, Davey G.

Int Health. 2018 Jul 1;10(4):285-293. doi: 10.1093/inthealth/ihy028.


Evaluation of in vitro culture systems for the maintenance of microfilariae and infective larvae of Loa loa.

Zofou D, Fombad FF, Gandjui NVT, Njouendou AJ, Kengne-Ouafo AJ, Chounna Ndongmo PW, Datchoua-Poutcheu FR, Enyong PA, Bita DT, Taylor MJ, Turner JD, Wanji S.

Parasit Vectors. 2018 May 2;11(1):275. doi: 10.1186/s13071-018-2852-2.


Heterogeneity in the in vitro susceptibility of Loa loa microfilariae to drugs commonly used in parasitological infections.

Njouendou AJ, Fombad FF, O'Neill M, Zofou D, Nutting C, Ndongmo PC, Kengne-Ouafo AJ, Geary TG, Mackenzie CD, Wanji S.

Parasit Vectors. 2018 Apr 4;11(1):223. doi: 10.1186/s13071-018-2799-3.


A Comparative Analysis of Economic Cost of Podoconiosis and Leprosy on Affected Households in the Northwest Region of Cameroon.

Tembei AM, Kengne-Ouaffo JA, Ngoh EA, John B, Nji TM, Deribe K, Enyong P, Nkuo-Akenji T, Davey G, Wanji S.

Am J Trop Med Hyg. 2018 Apr;98(4):1075-1081. doi: 10.4269/ajtmh.17-0931. Epub 2018 Feb 15.


Identifying co-endemic areas for major filarial infections in sub-Saharan Africa: seeking synergies and preventing severe adverse events during mass drug administration campaigns.

Cano J, Basáñez MG, O'Hanlon SJ, Tekle AH, Wanji S, Zouré HG, Rebollo MP, Pullan RL.

Parasit Vectors. 2018 Jan 31;11(1):70. doi: 10.1186/s13071-018-2655-5.


Author Correction: Short-Course, High-Dose Rifampicin Achieves Wolbachia Depletion Predictive of Curative Outcomes in Preclinical Models of Lymphatic Filariasis and Onchocerciasis.

Aljayyoussi G, Tyrer HE, Ford L, Sjoberg H, Pionnier N, Waterhouse D, Davies J, Gamble J, Metuge H, Cook DAN, Steven A, Sharma R, Guimaraes AF, Clare RH, Cassidy A, Johnston KL, Myhill L, Hayward L, Wanji S, Turner JD, Taylor MJ, Ward SA.

Sci Rep. 2018 Jan 18;8(1):1384. doi: 10.1038/s41598-018-19723-1.


Mapping the geographical distribution of podoconiosis in Cameroon using parasitological, serological, and clinical evidence to exclude other causes of lymphedema.

Deribe K, Beng AA, Cano J, Njouendo AJ, Fru-Cho J, Awah AR, Eyong ME, Chounna Ndongmo PW, Giorgi E, Pigott DM, Golding N, Pullan RL, Noor AM, Enquselassie F, Murray CJL, Brooker SJ, Hay SI, Enyong P, Newport MJ, Wanji S, Davey G.

PLoS Negl Trop Dis. 2018 Jan 11;12(1):e0006126. doi: 10.1371/journal.pntd.0006126. eCollection 2018 Jan.


Mansonella perstans microfilaremic individuals are characterized by enhanced type 2 helper T and regulatory T and B cell subsets and dampened systemic innate and adaptive immune responses.

Ritter M, Ndongmo WPC, Njouendou AJ, Nghochuzie NN, Nchang LC, Tayong DB, Arndts K, Nausch N, Jacobsen M, Wanji S, Layland LE, Hoerauf A.

PLoS Negl Trop Dis. 2018 Jan 11;12(1):e0006184. doi: 10.1371/journal.pntd.0006184. eCollection 2018 Jan.


Programmatic factors associated with the limited impact of Community-Directed Treatment with Ivermectin to control Onchocerciasis in three drainage basins of South West Cameroon.

Duamor CT, Datchoua-Poutcheu FR, Chounna Ndongmo WP, Yoah AT, Njukang E, Kah E, Maingeh MS, Kengne-Ouaffo JA, Tayong DB, Enyong PA, Wanji S.

PLoS Negl Trop Dis. 2017 Nov 20;11(11):e0005966. doi: 10.1371/journal.pntd.0005966. eCollection 2017 Nov.


Potential Role for Flubendazole in Limiting Filariasis Transmission: Observations of Microfilarial Sensitivity.

O'Neill M, Njouendou JA, Dzimianski M, Burkman E, Ndongmo PC, Kengne-Ouafo JA, Wanji S, Moorhead A, Mackenzie CD, Geary TG.

Am J Trop Med Hyg. 2018 Jan;98(1):21-26. doi: 10.4269/ajtmh.17-0390.


Successful long-term maintenance of Mansonella perstans in an in vitro culture system.

Njouendou AJ, Ritter M, Ndongmo WPC, Kien CA, Narcisse GTV, Fombad FF, Tayong DB, Pfarr K, Layland LE, Hoerauf A, Wanji S.

Parasit Vectors. 2017 Nov 10;10(1):563. doi: 10.1186/s13071-017-2515-8.


A Test-and-Not-Treat Strategy for Onchocerciasis in Loa loa-Endemic Areas.

Kamgno J, Pion SD, Chesnais CB, Bakalar MH, D'Ambrosio MV, Mackenzie CD, Nana-Djeunga HC, Gounoue-Kamkumo R, Njitchouang GR, Nwane P, Tchatchueng-Mbouga JB, Wanji S, Stolk WA, Fletcher DA, Klion AD, Nutman TB, Boussinesq M.

N Engl J Med. 2017 Nov 23;377(21):2044-2052. doi: 10.1056/NEJMoa1705026. Epub 2017 Nov 8.


Genome-wide analysis of ivermectin response by Onchocerca volvulus reveals that genetic drift and soft selective sweeps contribute to loss of drug sensitivity.

Doyle SR, Bourguinat C, Nana-Djeunga HC, Kengne-Ouafo JA, Pion SDS, Bopda J, Kamgno J, Wanji S, Che H, Kuesel AC, Walker M, Basáñez MG, Boakye DA, Osei-Atweneboana MY, Boussinesq M, Prichard RK, Grant WN.

PLoS Negl Trop Dis. 2017 Jul 26;11(7):e0005816. doi: 10.1371/journal.pntd.0005816. eCollection 2017 Jul.


Ivermectin treatment of Loa loa hyper-microfilaraemic baboons (Papio anubis): Assessment of microfilarial load reduction, haematological and biochemical parameters and histopathological changes following treatment.

Wanji S, Eyong EJ, Tendongfor N, Ngwa CJ, Esuka EN, Kengne-Ouafo AJ, Datchoua-Poutcheu FR, Enyong P, Agnew D, Eversole RR, Hopkins A, Mackenzie CD.

PLoS Negl Trop Dis. 2017 Jul 7;11(7):e0005576. doi: 10.1371/journal.pntd.0005576. eCollection 2017 Jul.


Validation of onchocerciasis biomarker N-acetyltyramine-O-glucuronide (NATOG).

Globisch D, Eubanks LM, Shirey RJ, Pfarr KM, Wanji S, Debrah AY, Hoerauf A, Janda KD.

Bioorg Med Chem Lett. 2017 Aug 1;27(15):3436-3440. doi: 10.1016/j.bmcl.2017.05.082. Epub 2017 May 29.


Short-Course, High-Dose Rifampicin Achieves Wolbachia Depletion Predictive of Curative Outcomes in Preclinical Models of Lymphatic Filariasis and Onchocerciasis.

Aljayyoussi G, Tyrer HE, Ford L, Sjoberg H, Pionnier N, Waterhouse D, Davies J, Gamble J, Metuge H, Cook DAN, Steven A, Sharma R, Guimaraes AF, Clare RH, Cassidy A, Johnston KL, Myhill L, Hayward L, Wanji S, Turner JD, Taylor MJ, Ward SA.

Sci Rep. 2017 Mar 16;7(1):210. doi: 10.1038/s41598-017-00322-5. Erratum in: Sci Rep. 2018 Jan 18;8(1):1384.


Determination of the predictive factors of long-lasting insecticide-treated net ownership and utilisation in the Bamenda Health District of Cameroon.

Fokam EB, Kindzeka GF, Ngimuh L, Dzi KT, Wanji S.

BMC Public Health. 2017 Mar 16;17(1):263. doi: 10.1186/s12889-017-4155-5.


Colorimetric tests for diagnosis of filarial infection and vector surveillance using non-instrumented nucleic acid loop-mediated isothermal amplification (NINA-LAMP).

Poole CB, Li Z, Alhassan A, Guelig D, Diesburg S, Tanner NA, Zhang Y, Evans TC Jr, LaBarre P, Wanji S, Burton RA, Carlow CK.

PLoS One. 2017 Feb 15;12(2):e0169011. doi: 10.1371/journal.pone.0169011. eCollection 2017.


Epidemiology of Mansonella perstans in the middle belt of Ghana.

Debrah LB, Nausch N, Opoku VS, Owusu W, Mubarik Y, Berko DA, Wanji S, Layland LE, Hoerauf A, Jacobsen M, Debrah AY, Phillips RO.

Parasit Vectors. 2017 Jan 7;10(1):15. doi: 10.1186/s13071-016-1960-0.


Using Community-Level Prevalence of Loa loa Infection to Predict the Proportion of Highly-Infected Individuals: Statistical Modelling to Support Lymphatic Filariasis and Onchocerciasis Elimination Programs.

Schlüter DK, Ndeffo-Mbah ML, Takougang I, Ukety T, Wanji S, Galvani AP, Diggle PJ.

PLoS Negl Trop Dis. 2016 Dec 1;10(12):e0005157. doi: 10.1371/journal.pntd.0005157. eCollection 2016 Dec.


Positivity of Antigen Tests Used for Diagnosis of Lymphatic Filariasis in Individuals Without Wuchereria bancrofti Infection But with High Loa loa Microfilaremia.

Pion SD, Montavon C, Chesnais CB, Kamgno J, Wanji S, Klion AD, Nutman TB, Boussinesq M.

Am J Trop Med Hyg. 2016 Dec 7;95(6):1417-1423. doi: 10.4269/ajtmh.16-0547. Epub 2016 Oct 10.


Detecting and staging podoconiosis cases in North West Cameroon: positive predictive value of clinical screening of patients by community health workers and researchers.

Wanji S, Kengne-Ouafo JA, Datchoua-Poutcheu FR, Njouendou AJ, Tayong DB, Sofeu-Feugaing DD, Amvongo-Adjia N, Fovennso BA, Longang-Tchounkeu YF, Tekola-Ayele F, Enyong PA, Newport MJ, Davey G.

BMC Public Health. 2016 Sep 20;16:997. doi: 10.1186/s12889-016-3669-6.


Tailoring mass drug administration to context: implementation research is critical in achieving equitable progress in the control and elimination of helminth neglected tropical diseases in sub-Saharan Africa.

Dean L, Page S, Hawkins K, Stothard R, Thomson R, Wanji S, Gyapong M, Anagbogu I, Molyneux D, Theobald S.

Int Health. 2016 Jul;8(4):233-4. doi: 10.1093/inthealth/ihw031.


Update on the distribution of Mansonella perstans in the southern part of Cameroon: influence of ecological factors and mass drug administration with ivermectin.

Wanji S, Tayong DB, Layland LE, Datchoua Poutcheu FR, Ndongmo WP, Kengne-Ouafo JA, Ritter M, Amvongo-Adjia N, Fombad FF, Njeshi CN, Nkwescheu AS, Enyong PA, Hoerauf A.

Parasit Vectors. 2016 May 31;9(1):311. doi: 10.1186/s13071-016-1595-1.


Stage-specific Proteomes from Onchocerca ochengi, Sister Species of the Human River Blindness Parasite, Uncover Adaptations to a Nodular Lifestyle.

Armstrong SD, Xia D, Bah GS, Krishna R, Ngangyung HF, LaCourse EJ, McSorley HJ, Kengne-Ouafo JA, Chounna-Ndongmo PW, Wanji S, Enyong PA, Taylor DW, Blaxter ML, Wastling JM, Tanya VN, Makepeace BL.

Mol Cell Proteomics. 2016 Aug;15(8):2554-75. doi: 10.1074/mcp.M115.055640. Epub 2016 May 25.


Further evidence of the cross-reactivity of the Binax NOW® Filariasis ICT cards to non-Wuchereria bancrofti filariae: experimental studies with Loa loa and Onchocerca ochengi.

Wanji S, Amvongo-Adjia N, Njouendou AJ, Kengne-Ouafo JA, Ndongmo WP, Fombad FF, Koudou B, Enyong PA, Bockarie M.

Parasit Vectors. 2016 May 5;9:267. doi: 10.1186/s13071-016-1556-8.

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