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Items: 1 to 50 of 70

1.

Diagnostic Accuracy and Utility of FluoroType MTBDR, a New Molecular Assay for Multidrug-Resistant Tuberculosis.

de Vos M, Derendinger B, Dolby T, Simpson J, van Helden PD, Rice JE, Wangh LJ, Theron G, Warren RM.

J Clin Microbiol. 2018 Aug 27;56(9). pii: e00531-18. doi: 10.1128/JCM.00531-18. Print 2018 Sep.

2.

Closed-Tube Barcoding.

Sirianni NM, Yuan H, Rice JE, Kaufman RS, Deng J, Fulton C, Wangh LJ.

Genome. 2016 Nov;59(11):1049-1061. Epub 2016 Aug 30.

3.

Low-concentration initiator primers improve the amplification of gene targets with high sequence variability.

Pierce KE, Wangh LJ.

Methods Mol Biol. 2015;1275:73-89. doi: 10.1007/978-1-4939-2365-6_5.

PMID:
25697652
4.
5.

Virtual Barcoding using LATE-PCR and Lights-On/Lights-Off probes: identification of nematode species in a closed-tube reaction.

Rice LM, Reis AH Jr, Wangh LJ.

Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(2):1358-63. doi: 10.3109/19401736.2014.947581. Epub 2014 Aug 11.

PMID:
25109627
6.

Kinetic hairpin oligonucleotide blockers for selective amplification of rare mutations.

Jia Y, Sanchez JA, Wangh LJ.

Sci Rep. 2014 Aug 1;4:5921. doi: 10.1038/srep05921.

7.

Construction of a microfluidic chip, using dried-down reagents, for LATE-PCR amplification and detection of single-stranded DNA.

Jia Y, Mak PI, Massey C, Martins RP, Wangh LJ.

Lab Chip. 2013 Dec 7;13(23):4635-41. doi: 10.1039/c3lc51049b.

PMID:
24113690
9.

Design and construction of a single tube, quantitative endpoint, LATE-PCR multiplex assay for ventilator-associated pneumonia.

Rice LM, Reis AH Jr, Mistry R, Khan H, Khosla P, Bharya S, Wangh LJ.

J Appl Microbiol. 2013 Sep;115(3):818-27. doi: 10.1111/jam.12281. Epub 2013 Jul 9.

10.

Rapid detection of TEM-type extended-spectrum β-lactamase (ESBL) mutations using lights-on/lights-off probes with single-stranded DNA amplification.

Pierce KE, Peter H, Bachmann TT, Volpe C, Mistry R, Rice JE, Wangh LJ.

J Mol Diagn. 2013 May;15(3):291-8. doi: 10.1016/j.jmoldx.2013.02.002. Epub 2013 Mar 19.

PMID:
23518216
11.

Design and Construction of a Single-Tube, LATE-PCR, Multiplex Endpoint Assay with Lights-On/Lights-Off Probes for the Detection of Pathogens Associated with Sepsis.

Carver-Brown RK, Reis AH Jr, Rice LM, Czajka JW, Wangh LJ.

J Pathog. 2012;2012:424808. doi: 10.1155/2012/424808. Epub 2012 Dec 26.

12.

Verification of monoplex and multiplex linear-after-the-exponential PCR gene-specific sepsis assays using clinical isolates.

Gentile NL, Dillier AM, Williams GV, Ackers J, Reis AH Jr, Rice LM, Wangh LJ, Czajka JW, Kost GJ.

J Appl Microbiol. 2013 Feb;114(2):586-94. doi: 10.1111/jam.12062. Epub 2013 Jan 7.

13.

Design of a single-tube, endpoint, linear-after-the-exponential-PCR assay for 17 pathogens associated with sepsis.

Rice LM, Reis AH Jr, Ronish B, Carver-Brown RK, Czajka JW, Gentile N, Kost G, Wangh LJ.

J Appl Microbiol. 2013 Feb;114(2):457-69. doi: 10.1111/jam.12061. Epub 2012 Dec 20.

14.

Rapid detection of sequence variation in Clostridium difficile genes using LATE-PCR with multiple mismatch-tolerant hybridization probes.

Pierce KE, Khan H, Mistry R, Goldenberg SD, French GL, Wangh LJ.

J Microbiol Methods. 2012 Nov;91(2):269-75. doi: 10.1016/j.mimet.2012.08.018. Epub 2012 Sep 7.

PMID:
22982259
15.

Fluorescent signatures for variable DNA sequences.

Rice JE, Reis AH Jr, Rice LM, Carver-Brown RK, Wangh LJ.

Nucleic Acids Res. 2012 Nov;40(21):e164. doi: 10.1093/nar/gks731. Epub 2012 Aug 8.

16.

Design and verification of a highly reliable Linear-After-The-Exponential PCR (LATE-PCR) assay for the detection of African swine fever virus.

Ronish B, Hakhverdyan M, Ståhl K, Gallardo C, Fernandez-Pinero J, Belák S, Leblanc N, Wangh L.

J Virol Methods. 2011 Mar;172(1-2):8-15. doi: 10.1016/j.jviromet.2010.12.003. Epub 2010 Dec 15. Erratum in: J Virol Methods. 2011 May;173(2):403. Gallardo, C [added]; Fernandez-Pinero, J [added].

PMID:
21167207
17.

LATE-PCR and allied technologies: real-time detection strategies for rapid, reliable diagnosis from single cells.

Pierce KE, Wangh LJ.

Methods Mol Biol. 2011;688:47-66. doi: 10.1007/978-1-60761-947-5_5.

PMID:
20938832
18.

Pan-serotypic detection of foot-and-mouth disease virus by RT linear-after-the-exponential PCR.

Reid SM, Pierce KE, Mistry R, Bharya S, Dukes JP, Volpe C, Wangh LJ, King DP.

Mol Cell Probes. 2010 Oct;24(5):250-5. doi: 10.1016/j.mcp.2010.04.004. Epub 2010 Apr 28.

PMID:
20433917
19.

Heat shock memory in preimplantation mouse embryos.

Jia Y, Hartshorn C, Hartung O, Wangh LJ.

Fertil Steril. 2010 May 15;93(8):2760-3. doi: 10.1016/j.fertnstert.2010.02.031. Epub 2010 Apr 7.

20.

One-step RT-LATE-PCR for mRNA and viral RNA detection and quantification.

Hartshorn C, Wangh LJ.

Methods Mol Biol. 2010;630:153-85. doi: 10.1007/978-1-60761-629-0_11.

PMID:
20300997
21.

Dilute-'N'-Go dideoxy sequencing of all DNA strands generated in multiplex LATE-PCR assays.

Jia Y, Osborne A, Rice JE, Wangh LJ.

Nucleic Acids Res. 2010 Jun;38(11):e119. doi: 10.1093/nar/gkq111. Epub 2010 Feb 26.

22.

Design and optimization of a novel reverse transcription linear-after-the-exponential PCR for the detection of foot-and-mouth disease virus.

Pierce KE, Mistry R, Reid SM, Bharya S, Dukes JP, Hartshorn C, King DP, Wangh LJ.

J Appl Microbiol. 2010 Jul;109(1):180-9. doi: 10.1111/j.1365-2672.2009.04640.x. Epub 2009 Nov 28.

23.

[Cigarette smoke-induced oxidative damage to human bronchial epithelial cells].

Liu SK, Wangh L, Chen C, Zhang ZZ.

Sichuan Da Xue Xue Bao Yi Xue Ban. 2009 Jul;40(4):667-71. Chinese.

PMID:
19764569
24.

Single-molecule LATE-PCR analysis of human mitochondrial genomic sequence variations.

Osborne A, Reis AH, Bach L, Wangh LJ.

PLoS One. 2009 May 20;4(5):e5636. doi: 10.1371/journal.pone.0005636.

25.

Single-cell duplex RT-LATE-PCR reveals Oct4 and Xist RNA gradients in 8-cell embryos.

Hartshorn C, Eckert JJ, Hartung O, Wangh LJ.

BMC Biotechnol. 2007 Dec 7;7:87.

26.

Monoplex/multiplex linear-after-the-exponential-PCR assays combined with PrimeSafe and Dilute-'N'-Go sequencing.

Rice JE, Sanchez JA, Pierce KE, Reis AH Jr, Osborne A, Wangh LJ.

Nat Protoc. 2007;2(10):2429-38.

PMID:
17947984
28.

Early onset of heat-shock response in mouse embryos revealed by quantification of stress-inducible hsp70i RNA.

Hartshorn C, Anshelevich A, Jia Y, Wangh LJ.

Gene Regul Syst Bio. 2007 Dec 6;1:365-73.

29.

Two-temperature LATE-PCR endpoint genotyping.

Sanchez JA, Abramowitz JD, Salk JJ, Reis AH Jr, Rice JE, Pierce KE, Wangh LJ.

BMC Biotechnol. 2006 Dec 4;6:44.

30.

Direct amplification of single-stranded DNA for pyrosequencing using linear-after-the-exponential (LATE)-PCR.

Salk JJ, Sanchez JA, Pierce KE, Rice JE, Soares KC, Wangh LJ.

Anal Biochem. 2006 Jun 1;353(1):124-32. Epub 2006 Feb 28.

31.

Laser zona drilling does not induce hsp70i transcription in blastomeres of eight-cell mouse embryos.

Hartshorn C, Anshelevich A, Wangh LJ.

Fertil Steril. 2005 Nov;84(5):1547-50.

PMID:
16275269
32.

Linear-After-The-Exponential (LATE)-PCR: primer design criteria for high yields of specific single-stranded DNA and improved real-time detection.

Pierce KE, Sanchez JA, Rice JE, Wangh LJ.

Proc Natl Acad Sci U S A. 2005 Jun 14;102(24):8609-14. Epub 2005 Jun 3.

33.
34.

Linear-after-the-exponential (LATE)-PCR: an advanced method of asymmetric PCR and its uses in quantitative real-time analysis.

Sanchez JA, Pierce KE, Rice JE, Wangh LJ.

Proc Natl Acad Sci U S A. 2004 Feb 17;101(7):1933-8. Epub 2004 Feb 9. Erratum in: Proc Natl Acad Sci U S A. 2009 Feb 10;106(6):2083.

35.

Effectiveness and limitations of uracil-DNA glycosylases in sensitive real-time PCR assays.

Pierce KE, Wangh LJ.

Biotechniques. 2004 Jan;36(1):44-6, 48. No abstract available.

36.

Detection of cystic fibrosis alleles from single cells using molecular beacons and a novel method of asymmetric real-time PCR.

Pierce KE, Rice JE, Sanchez JA, Wangh LJ.

Mol Hum Reprod. 2003 Dec;9(12):815-20.

PMID:
14614044
37.

Real-time PCR with molecular beacons provides a highly accurate assay for detection of Tay-Sachs alleles in single cells.

Rice JE, Sanchez JA, Pierce KE, Wangh LJ.

Prenat Diagn. 2002 Dec;22(12):1130-4.

PMID:
12454972
38.
39.

QuantiLyse: reliable DNA amplification from single cells.

Pierce KE, Rice JE, Sanchez JA, Wangh LJ.

Biotechniques. 2002 May;32(5):1106-11.

40.
41.

Nonrandom location and orientation of the inactive X chromosome in human neutrophil nuclei.

Karni RJ, Wangh LJ, Sanchez JA.

Chromosoma. 2001 Aug;110(4):267-74.

PMID:
11534818
42.

Real-time PCR using molecular beacons for accurate detection of the Y chromosome in single human blastomeres.

Pierce KE, Rice JE, Sanchez JA, Brenner C, Wangh LJ.

Mol Hum Reprod. 2000 Dec;6(12):1155-64.

PMID:
11101699
43.

Efficient human sperm pronucleus formation and replication in Xenopus egg extracts.

Neuber E, Havari E, Aquiles Sanchez J, Powers RD, Wangh LJ.

Biol Reprod. 1999 Oct;61(4):912-20.

PMID:
10491624
44.

New insights into the mechanisms of nuclear segmentation in human neutrophils.

Sanchez JA, Wangh LJ.

J Cell Biochem. 1999 Apr 1;73(1):1-10. Review.

PMID:
10088718
45.
46.

Efficient plasmid DNA replication in Xenopus egg extracts does not depend on prior chromatin assembly.

Aquiles Sanchez J, Wonsey DR, Harris L, Morales J, Wangh LJ.

J Biol Chem. 1995 Dec 15;270(50):29676-81.

47.

Efficient reactivation of Xenopus erythrocyte nuclei in Xenopus egg extracts.

Wangh LJ, DeGrace D, Sanchez JA, Gold A, Yeghiazarians Y, Wiedemann K, Daniels S.

J Cell Sci. 1995 Jun;108 ( Pt 6):2187-96.

48.

The view from Cairo.

Wangh LJ.

Brandeis Rev. 1995 Winter;15(2):23-7.

PMID:
12319267
49.

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