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Energetic adaptations: Metabolic control of endocytic membrane traffic.

Rahmani S, Defferrari MS, Wakarchuk WW, Antonescu CN.

Traffic. 2019 Dec;20(12):912-931. doi: 10.1111/tra.12705. Epub 2019 Nov 15. Review.


Comparison of α2,6-sialyltransferases for sialylation of therapeutic proteins.

Janesch B, Saxena H, Sim L, Wakarchuk WW.

Glycobiology. 2019 Sep 20;29(10):735-747. doi: 10.1093/glycob/cwz050.


Directed evolution of bacterial polysialyltransferases.

Janesch B, Baumann L, Mark A, Thompson N, Rahmani S, Sim L, Withers SG, Wakarchuk WW.

Glycobiology. 2019 Jul 1;29(7):588-598. doi: 10.1093/glycob/cwz021.


Assay Methods for the Glycosyltransferases Involved in Synthesis of Bacterial Polysaccharides.

Abukar T, Buenbrazo N, Janesch B, Kell L, Wakarchuk W.

Methods Mol Biol. 2019;1954:215-235. doi: 10.1007/978-1-4939-9154-9_17.


Genetics behind the Biosynthesis of Nonulosonic Acid-Containing Lipooligosaccharides in Campylobacter coli.

Kolehmainen A, Rossi M, Stupak J, Li J, Gilbert M, Wakarchuk W.

J Bacteriol. 2019 Mar 26;201(8). pii: e00759-18. doi: 10.1128/JB.00759-18. Print 2019 Apr 15.


A Bacterial Expression Platform for Production of Therapeutic Proteins Containing Human-like O-Linked Glycans.

Du T, Buenbrazo N, Kell L, Rahmani S, Sim L, Withers SG, DeFrees S, Wakarchuk W.

Cell Chem Biol. 2019 Feb 21;26(2):203-212.e5. doi: 10.1016/j.chembiol.2018.10.017. Epub 2018 Nov 29.


Characterization of a thermostable endoglucanase from Cellulomonas fimi ATCC484.

Saxena H, Hsu B, de Asis M, Zierke M, Sim L, Withers SG, Wakarchuk W.

Biochem Cell Biol. 2018 Feb;96(1):68-76. doi: 10.1139/bcb-2017-0150. Epub 2017 Oct 5.


X-ray crystallographic structure of a bacterial polysialyltransferase provides insight into the biosynthesis of capsular polysialic acid.

Lizak C, Worrall LJ, Baumann L, Pfleiderer MM, Volkers G, Sun T, Sim L, Wakarchuk W, Withers SG, Strynadka NCJ.

Sci Rep. 2017 Jul 19;7(1):5842. doi: 10.1038/s41598-017-05627-z.


Recognition of protein-linked glycans as a determinant of peptidase activity.

Noach I, Ficko-Blean E, Pluvinage B, Stuart C, Jenkins ML, Brochu D, Buenbrazo N, Wakarchuk W, Burke JE, Gilbert M, Boraston AB.

Proc Natl Acad Sci U S A. 2017 Jan 31;114(5):E679-E688. doi: 10.1073/pnas.1615141114. Epub 2017 Jan 17.


Construction of a hybrid β-hexosaminidase subunit capable of forming stable homodimers that hydrolyze GM2 ganglioside in vivo.

Tropak MB, Yonekawa S, Karumuthil-Melethil S, Thompson P, Wakarchuk W, Gray SJ, Walia JS, Mark BL, Mahuran D.

Mol Ther Methods Clin Dev. 2016 Mar 2;3:15057. doi: 10.1038/mtm.2015.57. eCollection 2016.


Proteomic Analysis of the Secretome of Cellulomonas fimi ATCC 484 and Cellulomonas flavigena ATCC 482.

Wakarchuk WW, Brochu D, Foote S, Robotham A, Saxena H, Erak T, Kelly J.

PLoS One. 2016 Mar 7;11(3):e0151186. doi: 10.1371/journal.pone.0151186. eCollection 2016.


Preparation of legionaminic acid analogs of sialo-glycoconjugates by means of mammalian sialyltransferases.

Watson DC, Wakarchuk WW, Gervais C, Durocher Y, Robotham A, Fernandes SM, Schnaar RL, Young NM, Gilbert M.

Glycoconj J. 2015 Dec;32(9):729-34. doi: 10.1007/s10719-015-9624-4. Epub 2015 Oct 9.


Structure of human ST8SiaIII sialyltransferase provides insight into cell-surface polysialylation.

Volkers G, Worrall LJ, Kwan DH, Yu CC, Baumann L, Lameignere E, Wasney GA, Scott NE, Wakarchuk W, Foster LJ, Withers SG, Strynadka NC.

Nat Struct Mol Biol. 2015 Aug;22(8):627-35. doi: 10.1038/nsmb.3060. Epub 2015 Jul 20.


Sialyltransferases with enhanced legionaminic acid transferase activity for the preparation of analogs of sialoglycoconjugates.

Watson DC, Wakarchuk WW, Leclerc S, Schur MJ, Schoenhofen IC, Young NM, Gilbert M.

Glycobiology. 2015 Jul;25(7):767-73. doi: 10.1093/glycob/cwv017. Epub 2015 Apr 3.


Characterization of five β-glycoside hydrolases from Cellulomonas fimi ATCC 484.

Gao J, Wakarchuk W.

J Bacteriol. 2014 Dec;196(23):4103-10. doi: 10.1128/JB.02194-14. Epub 2014 Sep 15.


A plate-based high-throughput activity assay for polysialyltransferase from Neisseria meningitidis.

Yu CC, Hill T, Kwan DH, Chen HM, Lin CC, Wakarchuk W, Withers SG.

Anal Biochem. 2014 Jan 1;444:67-74. doi: 10.1016/j.ab.2013.09.030. Epub 2013 Oct 10.


A glyco-gold nanoparticle based assay for α-2,8-polysialyltransferase from Neisseria meningitidis.

Yu CC, Huang LD, Kwan DH, Wakarchuk WW, Withers SG, Lin CC.

Chem Commun (Camb). 2013 Oct 3;49(86):10166-8. doi: 10.1039/c3cc45147j.


Biochemical characterization of a polysialyltransferase from Mannheimia haemolytica A2 and comparison to other bacterial polysialyltransferases.

Lindhout T, Bainbridge CR, Costain WJ, Gilbert M, Wakarchuk WW.

PLoS One. 2013 Jul 26;8(7):e69888. doi: 10.1371/journal.pone.0069888. Print 2013.


Review of phosphocholine substituents on bacterial pathogen glycans: synthesis, structures and interactions with host proteins.

Young NM, Foote SJ, Wakarchuk WW.

Mol Immunol. 2013 Dec;56(4):563-73. doi: 10.1016/j.molimm.2013.05.237. Epub 2013 Aug 1. Review.


In cellulo examination of a beta-alpha hybrid construct of beta-hexosaminidase A subunits, reported to interact with the GM2 activator protein and hydrolyze GM2 ganglioside.

Sinici I, Yonekawa S, Tkachyova I, Gray SJ, Samulski RJ, Wakarchuk W, Mark BL, Mahuran DJ.

PLoS One. 2013;8(3):e57908. doi: 10.1371/journal.pone.0057908. Epub 2013 Mar 4.


Structure-based mutagenic analysis of mechanism and substrate specificity in mammalian glycosyltransferases: porcine ST3Gal-I.

Rakic B, Rao FV, Freimann K, Wakarchuk W, Strynadka NC, Withers SG.

Glycobiology. 2013 May;23(5):536-45. doi: 10.1093/glycob/cwt001. Epub 2013 Jan 8.


Domain organization of the polymerizing mannosyltransferases involved in synthesis of the Escherichia coli O8 and O9a lipopolysaccharide O-antigens.

Greenfield LK, Richards MR, Vinogradov E, Wakarchuk WW, Lowary TL, Whitfield C.

J Biol Chem. 2012 Nov 2;287(45):38135-49. doi: 10.1074/jbc.M112.412577. Epub 2012 Sep 18.


Structures of Merkel cell polyomavirus VP1 complexes define a sialic acid binding site required for infection.

Neu U, Hengel H, Blaum BS, Schowalter RM, Macejak D, Gilbert M, Wakarchuk WW, Imamura A, Ando H, Kiso M, Arnberg N, Garcea RL, Peters T, Buck CB, Stehle T.

PLoS Pathog. 2012;8(7):e1002738. doi: 10.1371/journal.ppat.1002738. Epub 2012 Jul 26.


Biosynthesis of the polymannose lipopolysaccharide O-antigens from Escherichia coli serotypes O8 and O9a requires a unique combination of single- and multiple-active site mannosyltransferases.

Greenfield LK, Richards MR, Li J, Wakarchuk WW, Lowary TL, Whitfield C.

J Biol Chem. 2012 Oct 12;287(42):35078-91. doi: 10.1074/jbc.M112.401000. Epub 2012 Aug 8.


Enzymatic engineering of polysialic acid on cells in vitro and in vivo using a purified bacterial polysialyltransferase.

El Maarouf A, Moyo-Lee Yaw D, Lindhout T, Pearse DD, Wakarchuk W, Rutishauser U.

J Biol Chem. 2012 Sep 21;287(39):32770-9. Epub 2012 Jul 31.


Helicobacter pylori β1,3-N-acetylglucosaminyltransferase for versatile synthesis of type 1 and type 2 poly-LacNAcs on N-linked, O-linked and I-antigen glycans.

Peng W, Pranskevich J, Nycholat C, Gilbert M, Wakarchuk W, Paulson JC, Razi N.

Glycobiology. 2012 Nov;22(11):1453-64. doi: 10.1093/glycob/cws101. Epub 2012 Jul 11.


Thiogalactopyranosides are resistant to hydrolysis by α-galactosidases.

Adlercreutz D, Yoshimura Y, Mannerstedt K, Wakarchuk WW, Bennett EP, Dovichi NJ, Hindsgaul O, Palcic MM.

Chembiochem. 2012 Jul 23;13(11):1673-9. doi: 10.1002/cbic.201200155. Epub 2012 Jun 27.


Small molecules containing hetero-bicyclic ring systems compete with UDP-Glc for binding to WaaG glycosyltransferase.

Landström J, Persson K, Rademacher C, Lundborg M, Wakarchuk W, Peters T, Widmalm G.

Glycoconj J. 2012 Oct;29(7):491-502. doi: 10.1007/s10719-012-9411-4. Epub 2012 Jun 19.


Recognition of sialylated poly-N-acetyllactosamine chains on N- and O-linked glycans by human and avian influenza A virus hemagglutinins.

Nycholat CM, McBride R, Ekiert DC, Xu R, Rangarajan J, Peng W, Razi N, Gilbert M, Wakarchuk W, Wilson IA, Paulson JC.

Angew Chem Int Ed Engl. 2012 May 14;51(20):4860-3. doi: 10.1002/anie.201200596. Epub 2012 Apr 13.


Characterization of α2,3- and α2,6-sialyltransferases from Helicobacter acinonychis.

Schur MJ, Lameignere E, Strynadka NC, Wakarchuk WW.

Glycobiology. 2012 Jul;22(7):997-1006. doi: 10.1093/glycob/cws071. Epub 2012 Apr 14.


Identification and characterization of a lipopolysaccharide α,2,3-sialyltransferase from the human pathogen Helicobacter bizzozeronii.

Kondadi PK, Rossi M, Twelkmeyer B, Schur MJ, Li J, Schott T, Paulin L, Auvinen P, Hänninen ML, Schweda EK, Wakarchuk W.

J Bacteriol. 2012 May;194(10):2540-50. doi: 10.1128/JB.00126-12. Epub 2012 Mar 9.


Inhibition of galactosyltransferases by a novel class of donor analogues.

Descroix K, Pesnot T, Yoshimura Y, Gehrke SS, Wakarchuk W, Palcic MM, Wagner GK.

J Med Chem. 2012 Mar 8;55(5):2015-24. doi: 10.1021/jm201154p. Epub 2012 Feb 22.


Transition state analysis of Vibrio cholerae sialidase-catalyzed hydrolyses of natural substrate analogues.

Chan J, Lewis AR, Indurugalla D, Schur M, Wakarchuk W, Bennet AJ.

J Am Chem Soc. 2012 Feb 29;134(8):3748-57. doi: 10.1021/ja208564y. Epub 2012 Feb 17.


Structure and mechanism of the lipooligosaccharide sialyltransferase from Neisseria meningitidis.

Lin LY, Rakic B, Chiu CP, Lameignere E, Wakarchuk WW, Withers SG, Strynadka NC.

J Biol Chem. 2011 Oct 28;286(43):37237-48. doi: 10.1074/jbc.M111.249920. Epub 2011 Aug 31.


Structural and kinetic analysis of substrate binding to the sialyltransferase Cst-II from Campylobacter jejuni.

Lee HJ, Lairson LL, Rich JR, Lameignere E, Wakarchuk WW, Withers SG, Strynadka NC.

J Biol Chem. 2011 Oct 14;286(41):35922-32. doi: 10.1074/jbc.M111.261172. Epub 2011 Aug 5.


Site-specific enzymatic polysialylation of therapeutic proteins using bacterial enzymes.

Lindhout T, Iqbal U, Willis LM, Reid AN, Li J, Liu X, Moreno M, Wakarchuk WW.

Proc Natl Acad Sci U S A. 2011 May 3;108(18):7397-402. doi: 10.1073/pnas.1019266108. Epub 2011 Apr 18.


Genetics and molecular specificity of sialylation of Histophilus somni lipooligosaccharide (LOS) and the effect of LOS sialylation on Toll-like receptor-4 signaling.

Howard MD, Willis L, Wakarchuk W, St Michael F, Cox A, Horne WT, Hontecillas R, Bassaganya-Riera J, Lorenz E, Inzana TJ.

Vet Microbiol. 2011 Nov 21;153(1-2):163-72. doi: 10.1016/j.vetmic.2011.02.054. Epub 2011 Mar 5.


Enzymatic synthesis and properties of glycoconjugates with legionaminic acid as a replacement for neuraminic acid.

Watson DC, Leclerc S, Wakarchuk WW, Young NM.

Glycobiology. 2011 Jan;21(1):99-108. doi: 10.1093/glycob/cwq135. Epub 2010 Oct 25.


Fluorescence activated cell sorting as a general ultra-high-throughput screening method for directed evolution of glycosyltransferases.

Yang G, Rich JR, Gilbert M, Wakarchuk WW, Feng Y, Withers SG.

J Am Chem Soc. 2010 Aug 4;132(30):10570-7. doi: 10.1021/ja104167y.


A sensitive fluorescence-based assay for monitoring GM2 ganglioside hydrolysis in live patient cells and their lysates.

Tropak MB, Bukovac SW, Rigat BA, Yonekawa S, Wakarchuk W, Mahuran DJ.

Glycobiology. 2010 Mar;20(3):356-65. doi: 10.1093/glycob/cwp183. Epub 2009 Nov 16.


NMR spectroscopic characterization of the sialyltransferase CstII from Campylobacter jejuni: histidine 188 is the general base.

Chan PH, Lairson LL, Lee HJ, Wakarchuk WW, Strynadka NC, Withers SG, McIntosh LP.

Biochemistry. 2009 Dec 1;48(47):11220-30. doi: 10.1021/bi901606n.


Structural insight into mammalian sialyltransferases.

Rao FV, Rich JR, Rakić B, Buddai S, Schwartz MF, Johnson K, Bowe C, Wakarchuk WW, Defrees S, Withers SG, Strynadka NC.

Nat Struct Mol Biol. 2009 Nov;16(11):1186-8. doi: 10.1038/nsmb.1685. Epub 2009 Oct 11.


Mechanistic investigation of the endo-alpha-N-acetylgalactosaminidase from Streptococcus pneumoniae R6.

Willis LM, Zhang R, Reid A, Withers SG, Wakarchuk WW.

Biochemistry. 2009 Nov 3;48(43):10334-41. doi: 10.1021/bi9013825.


Structural and kinetic characterizations of the polysialic acid O-acetyltransferase OatWY from Neisseria meningitidis.

Lee HJ, Rakić B, Gilbert M, Wakarchuk WW, Withers SG, Strynadka NC.

J Biol Chem. 2009 Sep 4;284(36):24501-11. doi: 10.1074/jbc.M109.006049. Epub 2009 Jun 12.


A new sialidase mechanism: bacteriophage K1F endo-sialidase is an inverting glycosidase.

Morley TJ, Willis LM, Whitfield C, Wakarchuk WW, Withers SG.

J Biol Chem. 2009 Jun 26;284(26):17404-10. doi: 10.1074/jbc.M109.003970. Epub 2009 May 1.


Complete chemoenzymatic synthesis of the Forssman antigen using novel glycosyltransferases identified in Campylobacter jejuni and Pasteurella multocida.

Houliston RS, Bernatchez S, Karwaski MF, Mandrell RE, Jarrell HC, Wakarchuk WW, Gilbert M.

Glycobiology. 2009 Feb;19(2):153-9. doi: 10.1093/glycob/cwn117. Epub 2008 Oct 25.


The structural basis for T-antigen hydrolysis by Streptococcus pneumoniae: a target for structure-based vaccine design.

Caines ME, Zhu H, Vuckovic M, Willis LM, Withers SG, Wakarchuk WW, Strynadka NC.

J Biol Chem. 2008 Nov 14;283(46):31279-83. doi: 10.1074/jbc.C800150200. Epub 2008 Sep 10.


Chemo-enzymatic synthesis of poly-N-acetyllactosamine (poly-LacNAc) structures and their characterization for CGL2-galectin-mediated binding of ECM glycoproteins to biomaterial surfaces.

Sauerzapfe B, Krenek K, Schmiedel J, Wakarchuk WW, Pelantová H, Kren V, Elling L.

Glycoconj J. 2009 Feb;26(2):141-59. doi: 10.1007/s10719-008-9172-2. Epub 2008 Aug 29.


A beta-1,4-galactosyltransferase from Helicobacter pylori is an efficient and versatile biocatalyst displaying a novel activity for thioglycoside synthesis.

Namdjou DJ, Chen HM, Vinogradov E, Brochu D, Withers SG, Wakarchuk WW.

Chembiochem. 2008 Jul 2;9(10):1632-40. doi: 10.1002/cbic.200700775.


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