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Structure and function of lipid A-modifying enzymes.

Anandan A, Vrielink A.

Ann N Y Acad Sci. 2019 Sep 25. doi: 10.1111/nyas.14244. [Epub ahead of print] Review.


Editorial overview: Catalysis and regulation: Structural features guiding enzyme catalysed processes.

Vrielink A, Holden HM.

Curr Opin Struct Biol. 2018 Dec;53:iii-v. doi: 10.1016/ No abstract available.


Enzyme targets for drug design of new anti-virulence therapeutics.

Kahler CM, Sarkar-Tyson M, Kibble EA, Stubbs KA, Vrielink A.

Curr Opin Struct Biol. 2018 Dec;53:140-150. doi: 10.1016/ Epub 2018 Sep 14. Review.


Structure-Function Relationships of the Neisserial EptA Enzyme Responsible for Phosphoethanolamine Decoration of Lipid A: Rationale for Drug Targeting.

Kahler CM, Nawrocki KL, Anandan A, Vrielink A, Shafer WM.

Front Microbiol. 2018 Aug 21;9:1922. doi: 10.3389/fmicb.2018.01922. eCollection 2018. Review.


The first transcriptomes from field-collected individual whiteflies ( Bemisia tabaci, Hemiptera: Aleyrodidae):  a case study of the endosymbiont composition.

Sseruwagi P, Wainaina J, Ndunguru J, Tumuhimbise R, Tairo F, Guo JY, Vrielink A, Blythe A, Kinene T, De Marchi B, Kehoe MA, Tanz S, Boykin LM.

Version 3. Gates Open Res. 2018 Mar 8 [revised 2018 Jan 1];1:16. doi: 10.12688/gatesopenres.12783.3. eCollection 2017.


Computational insights for the hydride transfer and distinctive roles of key residues in cholesterol oxidase.

Yu LJ, Golden E, Chen N, Zhao Y, Vrielink A, Karton A.

Sci Rep. 2017 Dec 8;7(1):17265. doi: 10.1038/s41598-017-17503-x.


Computational site-directed mutagenesis studies of the role of the hydrophobic triad on substrate binding in cholesterol oxidase.

Harb LH, Arooj M, Vrielink A, Mancera RL.

Proteins. 2017 Sep;85(9):1645-1655. doi: 10.1002/prot.25319. Epub 2017 May 25.


Acquisition of the capsule locus by horizontal gene transfer in Neisseria meningitidis is often accompanied by the loss of UDP-GalNAc synthesis.

Bartley SN, Mowlaboccus S, Mullally CA, Stubbs KA, Vrielink A, Maiden MC, Harrison OB, Perkins TT, Kahler CM.

Sci Rep. 2017 Mar 14;7:44442. doi: 10.1038/srep44442.


Structure of a lipid A phosphoethanolamine transferase suggests how conformational changes govern substrate binding.

Anandan A, Evans GL, Condic-Jurkic K, O'Mara ML, John CM, Phillips NJ, Jarvis GA, Wills SS, Stubbs KA, Moraes I, Kahler CM, Vrielink A.

Proc Natl Acad Sci U S A. 2017 Feb 28;114(9):2218-2223. doi: 10.1073/pnas.1612927114. Epub 2017 Feb 13.


Direct demonstration of lipid phosphorylation in the lipid bilayer of the biomimetic bicontinuous cubic phase using the confined enzyme lipid A phosphoethanolamine transferase.

van 't Hag L, Anandan A, Seabrook SA, Gras SL, Drummond CJ, Vrielink A, Conn CE.

Soft Matter. 2017 Feb 15;13(7):1493-1504. doi: 10.1039/c6sm02487d.


An extended N-H bond, driven by a conserved second-order interaction, orients the flavin N5 orbital in cholesterol oxidase.

Golden E, Yu LJ, Meilleur F, Blakeley MP, Duff AP, Karton A, Vrielink A.

Sci Rep. 2017 Jan 18;7:40517. doi: 10.1038/srep40517.


Detergents in Membrane Protein Purification and Crystallisation.

Anandan A, Vrielink A.

Adv Exp Med Biol. 2016;922:13-28. doi: 10.1007/978-3-319-35072-1_2.


The yeast transcription elongation factor Spt4/5 is a sequence-specific RNA binding protein.

Blythe AJ, Yazar-Klosinski B, Webster MW, Chen E, Vandevenne M, Bendak K, Mackay JP, Hartzog GA, Vrielink A.

Protein Sci. 2016 Sep;25(9):1710-21. doi: 10.1002/pro.2976. Epub 2016 Jul 15.


The Design and Structure of Outer Membrane Receptors from Peroxisomes, Mitochondria, and Chloroplasts.

Panigrahi R, Kubiszewski-Jakubiak S, Whelan J, Vrielink A.

Structure. 2015 Oct 6;23(10):1783-1800. doi: 10.1016/j.str.2015.08.005. Epub 2015 Sep 10. Review.


Production and characterization of recombinant perdeuterated cholesterol oxidase.

Golden E, Attwood PV, Duff AP, Meilleur F, Vrielink A.

Anal Biochem. 2015 Sep 15;485:102-8. doi: 10.1016/j.ab.2015.06.008. Epub 2015 Jun 11.


Cholesterol oxidase: ultrahigh-resolution crystal structure and multipolar atom model-based analysis.

Zarychta B, Lyubimov A, Ahmed M, Munshi P, Guillot B, Vrielink A, Jelsch C.

Acta Crystallogr D Biol Crystallogr. 2015 Apr;71(Pt 4):954-68. doi: 10.1107/S1399004715002382. Epub 2015 Mar 27.


High-resolution structures of cholesterol oxidase in the reduced state provide insights into redox stabilization.

Golden E, Karton A, Vrielink A.

Acta Crystallogr D Biol Crystallogr. 2014 Dec 1;70(Pt 12):3155-66. doi: 10.1107/S139900471402286X. Epub 2014 Nov 22.


The role of oxidoreductases in determining the function of the neisserial lipid A phosphoethanolamine transferase required for resistance to polymyxin.

Piek S, Wang Z, Ganguly J, Lakey AM, Bartley SN, Mowlaboccus S, Anandan A, Stubbs KA, Scanlon MJ, Vrielink A, Azadi P, Carlson RW, Kahler CM.

PLoS One. 2014 Sep 12;9(9):e106513. doi: 10.1371/journal.pone.0106513. eCollection 2014.


Ubiquitin fusion constructs allow the expression and purification of multi-KOW domain complexes of the Saccharomyces cerevisiae transcription elongation factor Spt4/5.

Blythe A, Gunasekara S, Walshe J, Mackay JP, Hartzog GA, Vrielink A.

Protein Expr Purif. 2014 Aug;100:54-60. doi: 10.1016/j.pep.2014.05.005. Epub 2014 May 21.


Binding and channeling of alternative substrates in the enzyme DmpFG: a molecular dynamics study.

Smith NE, Vrielink A, Attwood PV, Corry B.

Biophys J. 2014 Apr 15;106(8):1681-90. doi: 10.1016/j.bpj.2014.03.013.


Exploring ligand recognition, selectivity and dynamics of TPR domains of chloroplast Toc64 and mitochondria Om64 from Arabidopsis thaliana.

Panigrahi R, Whelan J, Vrielink A.

J Mol Recognit. 2014 Jun;27(6):402-14. doi: 10.1002/jmr.2360.


Ligand recognition by the TPR domain of the import factor Toc64 from Arabidopsis thaliana.

Panigrahi R, Adina-Zada A, Whelan J, Vrielink A.

PLoS One. 2013 Dec 31;8(12):e83461. doi: 10.1371/journal.pone.0083461. eCollection 2013.


The structure of the neisserial lipooligosaccharide phosphoethanolamine transferase A (LptA) required for resistance to polymyxin.

Wanty C, Anandan A, Piek S, Walshe J, Ganguly J, Carlson RW, Stubbs KA, Kahler CM, Vrielink A.

J Mol Biol. 2013 Sep 23;425(18):3389-402. doi: 10.1016/j.jmb.2013.06.029. Epub 2013 Jun 28.


Mechanism of the dehydrogenase reaction of DmpFG and analysis of inter-subunit channeling efficiency and thermodynamic parameters in the overall reaction.

Smith NE, Tie WJ, Flematti GR, Stubbs KA, Corry B, Attwood PV, Vrielink A.

Int J Biochem Cell Biol. 2013 Aug;45(8):1878-85. doi: 10.1016/j.biocel.2013.05.028. Epub 2013 Jun 3.


Structure of a class III engineered cephalosporin acylase: comparisons with class I acylase and implications for differences in substrate specificity and catalytic activity.

Golden E, Paterson R, Tie WJ, Anandan A, Flematti G, Molla G, Rosini E, Pollegioni L, Vrielink A.

Biochem J. 2013 Apr 15;451(2):217-26. doi: 10.1042/BJ20121715.


Cloning, expression, purification and crystallization of an endotoxin-biosynthesis enzyme from Neisseria meningitidis.

Anandan A, Piek S, Kahler CM, Vrielink A.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Dec 1;68(Pt 12):1494-7. doi: 10.1107/S1744309112042236. Epub 2012 Nov 14.


Biological channeling of a reactive intermediate in the bifunctional enzyme DmpFG.

Smith NE, Vrielink A, Attwood PV, Corry B.

Biophys J. 2012 Feb 22;102(4):868-77. doi: 10.1016/j.bpj.2012.01.029. Epub 2012 Feb 21.


Enzymatic toxins from snake venom: structural characterization and mechanism of catalysis.

Kang TS, Georgieva D, Genov N, Murakami MT, Sinha M, Kumar RP, Kaur P, Kumar S, Dey S, Sharma S, Vrielink A, Betzel C, Takeda S, Arni RK, Singh TP, Kini RM.

FEBS J. 2011 Dec;278(23):4544-76. doi: 10.1111/j.1742-4658.2011.08115.x. Epub 2011 May 17. Review.


Conserved and novel functions for Arabidopsis thaliana MIA40 in assembly of proteins in mitochondria and peroxisomes.

Carrie C, Giraud E, Duncan O, Xu L, Wang Y, Huang S, Clifton R, Murcha M, Filipovska A, Rackham O, Vrielink A, Whelan J.

J Biol Chem. 2010 Nov 12;285(46):36138-48. doi: 10.1074/jbc.M110.121202. Epub 2010 Sep 9.


Crystallization and preliminary diffraction analysis of an engineered cephalosporin acylase.

Anandan A, Vallet C, Coyle T, Moustafa IM, Vrielink A.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Jul 1;66(Pt 7):808-10. doi: 10.1107/S1744309110017185. Epub 2010 Jun 24.


Preliminary studies into the inhibition of the cholesterol alpha-glucosyltransferase from Helicobacter pylori using azasugars.

Gunasekara S, Vrielink A, Stubbs KA.

Carbohydr Res. 2010 May 7;345(7):960-4. doi: 10.1016/j.carres.2010.03.003. Epub 2010 Mar 4.


Cholesterol oxidase: structure and function.

Vrielink A.

Subcell Biochem. 2010;51:137-58. doi: 10.1007/978-90-481-8622-8_5. Review.


A hydrogen-bonding network is important for oxidation and isomerization in the reaction catalyzed by cholesterol oxidase.

Lyubimov AY, Chen L, Sampson NS, Vrielink A.

Acta Crystallogr D Biol Crystallogr. 2009 Nov;65(Pt 11):1222-31. doi: 10.1107/S0907444909037421. Epub 2009 Oct 22.


Cholesterol oxidase: biochemistry and structural features.

Vrielink A, Ghisla S.

FEBS J. 2009 Dec;276(23):6826-43. doi: 10.1111/j.1742-4658.2009.07377.x. Epub 2009 Oct 16. Review.


The binding and release of oxygen and hydrogen peroxide are directed by a hydrophobic tunnel in cholesterol oxidase.

Chen L, Lyubimov AY, Brammer L, Vrielink A, Sampson NS.

Biochemistry. 2008 May 13;47(19):5368-77. doi: 10.1021/bi800228w. Epub 2008 Apr 15.


Distortion of flavin geometry is linked to ligand binding in cholesterol oxidase.

Lyubimov AY, Heard K, Tang H, Sampson NS, Vrielink A.

Protein Sci. 2007 Dec;16(12):2647-56.


Crystal structure of LAAO from Calloselasma rhodostoma with an L-phenylalanine substrate: insights into structure and mechanism.

Moustafa IM, Foster S, Lyubimov AY, Vrielink A.

J Mol Biol. 2006 Dec 15;364(5):991-1002. Epub 2006 Sep 16.


Structural and kinetic analyses of the H121A mutant of cholesterol oxidase.

Lim L, Molla G, Guinn N, Ghisla S, Pollegioni L, Vrielink A.

Biochem J. 2006 Nov 15;400(1):13-22.


Atomic resolution crystallography reveals how changes in pH shape the protein microenvironment.

Lyubimov AY, Lario PI, Moustafa I, Vrielink A.

Nat Chem Biol. 2006 May;2(5):259-64. Epub 2006 Apr 9. Erratum in: Nat Chem Biol. 2006 Jun;2(6):346.


Sub-Angstrom resolution enzyme X-ray structures: is seeing believing?

Vrielink A, Sampson N.

Curr Opin Struct Biol. 2003 Dec;13(6):709-15. Review.


Cholesterol oxidases: a study of nature's approach to protein design.

Sampson NS, Vrielink A.

Acc Chem Res. 2003 Sep;36(9):713-22. Review.


Crystal structure of a bifunctional aldolase-dehydrogenase: sequestering a reactive and volatile intermediate.

Manjasetty BA, Powlowski J, Vrielink A.

Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):6992-7. Epub 2003 May 22.


A novel type of regulation of the vimentin intermediate filament cytoskeleton by a Golgi protein.

Gao YS, Vrielink A, MacKenzie R, Sztul E.

Eur J Cell Biol. 2002 Jul;81(7):391-401.


Molecular features of the copper binding sites in the octarepeat domain of the prion protein.

Burns CS, Aronoff-Spencer E, Dunham CM, Lario P, Avdievich NI, Antholine WE, Olmstead MM, Vrielink A, Gerfen GJ, Peisach J, Scott WG, Millhauser GL.

Biochemistry. 2002 Mar 26;41(12):3991-4001.


Purification and characterization of the human PDE4A catalytic domain (PDE4A330-723) expressed in Sf9 cells.

Lario PI, Bobechko B, Bateman K, Kelly J, Vrielink A, Huang Z.

Arch Biochem Biophys. 2001 Oct 1;394(1):54-60.


Structure and characterization of the glycan moiety of L-amino-acid oxidase from the Malayan pit viper Calloselasma rhodostoma.

Geyer A, Fitzpatrick TB, Pawelek PD, Kitzing K, Vrielink A, Ghisla S, Macheroux P.

Eur J Biochem. 2001 Jul;268(14):4044-53.


Oxygen access to the active site of cholesterol oxidase through a narrow channel is gated by an Arg-Glu pair.

Coulombe R, Yue KQ, Ghisla S, Vrielink A.

J Biol Chem. 2001 Aug 10;276(32):30435-41. Epub 2001 Jun 7.

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