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J Exp Med. 2018 Apr 2;215(4):1115-1133. doi: 10.1084/jem.20171608. Epub 2018 Mar 6.

Unveiling skin macrophage dynamics explains both tattoo persistence and strenuous removal.

Author information

1
Centre d'Immunologie de Marseille-Luminy, Aix Marseille Université, INSERM, CNRS UMR, Marseille, France.
2
UMR3215, Institut Curie, Paris, France.
3
Centre d'Immunophénomique, Aix Marseille Université, INSERM, CNRS, Marseille, France.
4
Institut Curie, PSL Research University, INSERM U932, Paris, France.
5
Centre d'Immunologie de Marseille-Luminy, Aix Marseille Université, INSERM, CNRS UMR, Marseille, France henri@ciml.univ-mrs.fr.
6
Centre d'Immunologie de Marseille-Luminy, Aix Marseille Université, INSERM, CNRS UMR, Marseille, France bernardm@ciml.univ-mrs.fr.

Abstract

Here we describe a new mouse model that exploits the pattern of expression of the high-affinity IgG receptor (CD64) and allows diphtheria toxin (DT)-mediated ablation of tissue-resident macrophages and monocyte-derived cells. We found that the myeloid cells of the ear skin dermis are dominated by DT-sensitive, melanin-laden cells that have been missed in previous studies and correspond to macrophages that have ingested melanosomes from neighboring melanocytes. Those cells have been referred to as melanophages in humans. We also identified melanophages in melanocytic melanoma. Benefiting of our knowledge on melanophage dynamics, we determined the identity, origin, and dynamics of the skin myeloid cells that capture and retain tattoo pigment particles. We showed that they are exclusively made of dermal macrophages. Using the possibility to delete them, we further demonstrated that tattoo pigment particles can undergo successive cycles of capture-release-recapture without any tattoo vanishing. Therefore, congruent with dermal macrophage dynamics, long-term tattoo persistence likely relies on macrophage renewal rather than on macrophage longevity.

PMID:
29511065
PMCID:
PMC5881467
[Available on 2018-10-02]
DOI:
10.1084/jem.20171608

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