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Antimicrob Agents Chemother. 2018 Nov 26;62(12). pii: e00845-18. doi: 10.1128/AAC.00845-18. Print 2018 Dec.

Dried Blood Spot Technique-Based Liquid Chromatography-Tandem Mass Spectrometry Method as a Simple Alternative for Benznidazole Pharmacokinetic Assessment.

Author information

1
Núcleo de Desenvolvimento Farmacêutico e Cosmético/Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil danilo.bedor@nudfac.com.br.
2
B&S Inovação em Desenvolvimento e Análise de Produtos Farmacêuticos, Recife, Pernambuco, Brazil.
3
Núcleo de Desenvolvimento Farmacêutico e Cosmético/Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
4
Parasitology and Chagas Service, Buenos Aires Children's Hospital Ricardo Gutierrez, Buenos Aires, Argentina.
5
Drugs for Neglected Diseases Initiative (DNDi), Geneva, Switzerland.

Abstract

Chagas disease (CD) is recognized as one of the major neglected global tropical diseases. Benznidazole (BNZ) is the drug of choice for the treatment of adults, young infants, and newborns with CD. However, the pharmacokinetics (PK) of BNZ have been poorly evaluated in all age groups, with consequent gaps in knowledge about PK-pharmacodynamic relationships in CD. The purpose of this study was to develop and validate a bioanalytical method to quantify BNZ levels in small-volume whole-blood samples collected as dried blood spots (DBS). The analysis was performed using high-performance liquid chromatography-positive electrospray tandem mass spectrometry. PK evaluation in healthy male volunteers was conducted to verify the correlation between DBS and plasma BNZ concentrations. The calibration curve was linear from 50 to 20,000 ng · ml-1 Intra- and interday precision and bias values were less than 14.87% (n = 9) and 9.81% (n = 27), respectively. The recovery rates ranged from 94 to 100% with no matrix effect. There was no hematocrit level effect in a range of 20 to 70%. The PK results obtained from DBS and plasma were comparable (r 2 = 0.8295) and equivalent to previously published information on BNZ. BNZ in DBS was stable at room temperature for more than one year. This article describes the first microsampling method for measuring BNZ levels in DBS that has the potential to facilitate broad implementation of PK in clinical trials involving adult and pediatric patients in remote areas and helps to address existing knowledge gaps in the treatment of CD.

KEYWORDS:

LC-MS/MS; benznidazole; dried blood spots; pharmacokinetics

PMID:
30275095
PMCID:
PMC6256767
DOI:
10.1128/AAC.00845-18
[Indexed for MEDLINE]
Free PMC Article

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