Format

Send to

Choose Destination
Sci Transl Med. 2018 Aug 8;10(453). pii: eaao6299. doi: 10.1126/scitranslmed.aao6299.

MicroRNA-30c-5p modulates neuropathic pain in rodents.

Author information

1
Departamento de Fisiología y Farmacología, Universidad de Cantabria, E-39011 Santander, Spain.
2
Instituto de Investigación Valdecilla, E-39011 Santander, Spain.
3
Servicio de Anestesiología, Hospital Universitario Valdecilla, E-39008 Santander, Spain.
4
Departamento de Ciencias Médicas y Quirúrgicas, Universidad de Cantabria, E-39011 Santander, Spain.
5
CIBER de Epidemiología y Salud Pública, Spain.
6
Departamento de Fisiología y Farmacología, Universidad de Cantabria, E-39011 Santander, Spain. hurlem@unican.es.

Abstract

Neuropathic pain is a debilitating chronic syndrome that is often refractory to currently available analgesics. Aberrant expression of several microRNAs (miRNAs) in nociception-related neural structures is associated with neuropathic pain in rodent models. We have exploited the antiallodynic phenotype of mice lacking the bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI), a transforming growth factor-β (TGF-β) pseudoreceptor. We used these mice to identify new miRNAs that might be useful for diagnosing, treating, or predicting neuropathic pain. We show that, after sciatic nerve injury in rats, miR-30c-5p was up-regulated in the spinal cord, dorsal root ganglia, cerebrospinal fluid (CSF) and plasma and that the expression of miR-30c-5p positively correlated with the severity of allodynia. The administration of a miR-30c-5p inhibitor into the cisterna magna of the brain delayed neuropathic pain development and reversed fully established allodynia in rodents. The mechanism was mediated by TGF-β and involved the endogenous opioid system. In patients with neuropathic pain associated with leg ischemia, the expression of miR-30c-5p was increased in plasma and CSF compared to control patients without pain. Logistic regression analysis in our cohort of patients showed that the expression of miR-30c-5p in plasma and CSF, in combination with other clinical variables, might be useful to help to predict neuropathic pain occurrence in patients with chronic peripheral ischemia.

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center