Format

Send to

Choose Destination
Nat Commun. 2019 Nov 22;10(1):5327. doi: 10.1038/s41467-019-13327-7.

Dynamic constriction and fission of endoplasmic reticulum membranes by reticulon.

Author information

1
Biofisika Institute (CSIC, UPV/EHU) and Department of Biochemistry and Molecular, Biology, University of the Basque Country, Leioa, 48940, Spain.
2
Scientific Institute, IRCCS E. Medea, Laboratory of Molecular Biology, Bosisio Parini, Lecco, Italy.
3
Neuroscience Institute, Italian National Research Council (CNR), Padova, Italy.
4
IMDEA Nanociencia, C/Faraday 9, Ciudad Universitaria de Cantoblanco, 28049, Madrid, Spain.
5
Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy.
6
Nanobiotecnología (IMDEA-Nanociencia) Unidad Asociada al Centro Nacional de Biotecnologia (CSIC), 28049, Madrid, Spain.
7
A.N. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, Moscow, 119071, Russia.
8
Federal Research and Clinical Centre of Physical-Chemical Medicine, Moscow, 119435, Russia.
9
Biofisika Institute (CSIC, UPV/EHU) and Department of Biochemistry and Molecular, Biology, University of the Basque Country, Leioa, 48940, Spain. vadim.frolov@ehu.eus.
10
IKERBASQUE, Basque Foundation for Science, Bilbao, 48013, Spain. vadim.frolov@ehu.eus.
11
Scientific Institute, IRCCS E. Medea, Laboratory of Molecular Biology, Bosisio Parini, Lecco, Italy. andrea.daga@gmail.com.

Abstract

The endoplasmic reticulum (ER) is a continuous cell-wide membrane network. Network formation has been associated with proteins producing membrane curvature and fusion, such as reticulons and atlastin. Regulated network fragmentation, occurring in different physiological contexts, is less understood. Here we find that the ER has an embedded fragmentation mechanism based upon the ability of reticulon to produce fission of elongating network branches. In Drosophila, Rtnl1-facilitated fission is counterbalanced by atlastin-driven fusion, with the prevalence of Rtnl1 leading to ER fragmentation. Ectopic expression of Drosophila reticulon in COS-7 cells reveals individual fission events in dynamic ER tubules. Consistently, in vitro analyses show that reticulon produces velocity-dependent constriction of lipid nanotubes leading to stochastic fission via a hemifission mechanism. Fission occurs at elongation rates and pulling force ranges intrinsic to the ER, thus suggesting a principle whereby the dynamic balance between fusion and fission controlling organelle morphology depends on membrane motility.

PMID:
31757972
PMCID:
PMC6876568
DOI:
10.1038/s41467-019-13327-7
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center