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Genetics. 2018 Aug;209(4):1197-1224. doi: 10.1534/genetics.118.300985. Epub 2018 Jun 25.

Initiation of Meiotic Development Is Controlled by Three Post-transcriptional Pathways in Caenorhabditis elegans.

Author information

1
Department of Genetics, School of Medicine, Washington University in St. Louis, Missouri 63110.
2
Department of Biological Sciences, University of Calgary, T2N 1N4, Canada.
3
Department of Chromosome Biology, Max F. Perutz Laboratories, University of Vienna, Vienna Biocenter, 1030, Austria.
4
Department of Genetics, School of Medicine, Washington University in St. Louis, Missouri 63110 ts@genetics.wustl.edu.

Abstract

A major event in germline development is the transition from stem/progenitor cells to entry into meiosis and gametogenesis. This transition requires downregulation of mitotic cell cycle activity and upregulation of processes associated with meiosis. We identify the Caenorhabditis elegans SCFPROM-1 E3 ubiquitin-ligase complex as functioning to downregulate mitotic cell cycle protein levels including cyclin E, WAPL-1, and KNL-2 at meiotic entry and, independently, promoting homologous chromosome pairing as a positive regulator of the CHK-2 kinase. SCFPROM-1 is thus a novel regulator of meiotic entry, coordinating downregulation of mitotic cell cycle proteins and promoting homolog pairing. We further show that SCFPROM-1 functions redundantly, in parallel to the previously described GLD-1 and GLD-2 meiotic entry pathways, downstream of and inhibited by GLP-1 Notch signaling, which specifies the stem cell fate. Accordingly, C. elegans employs three post-transcriptional pathways, SCFPROM-1-mediated protein degradation, GLD-1-mediated translational repression, and GLD-2-mediated translational activation, to control and coordinate the initiation of meiotic development.

KEYWORDS:

GLD-1; GLD-2; PROM-1; SCF; germline; meiotic development; meiotic entry

PMID:
29941619
PMCID:
PMC6063227
DOI:
10.1534/genetics.118.300985
[Indexed for MEDLINE]
Free PMC Article

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