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Optimization and evaluation of a novel real-time RT-PCR test for detection of parechovirus in cerebrospinal fluid.

Liu B, Forman M, Valsamakis A.

J Virol Methods. 2019 Oct;272:113690. doi: 10.1016/j.jviromet.2019.113690. Epub 2019 Jul 5.


Linkage to Care, Antiretroviral Treatment Initiation, and Viral Suppression of Acute HIV-Infected Individuals Identified From an Emergency Department-Based HIV Screening and Linkage-to-Care Program.

Johns Hopkins Hospital Emergency Department HIV Screening and Linkage-to-Care Team, Negoita S, Rothman RE, Signer D, Whalen M, Ricketts E, Saheed M, Hsieh YH, Rothman RE, Jones JL, Nolan K, Valsamakis A, Chaulk P, Olthoff G.

Ann Emerg Med. 2018 Nov;72(5):621-623. doi: 10.1016/j.annemergmed.2018.07.001. No abstract available.


Diagnosis and monitoring of HCV infection using the cobas® HCV test for use on the cobas® 6800/8800 systems.

Yao JD, Young S, Heilek GM, Marino E, Paxinos EE, Marins EG, Valsamakis A.

J Clin Virol. 2018 May;102:63-69. doi: 10.1016/j.jcv.2018.02.017. Epub 2018 Feb 24.


Comparison of Hepatitis B Virus Infection in HIV-Infected and HIV-Uninfected Participants Enrolled in a Multinational Clinical Trial: HPTN 052.

Greer AE, Ou SS, Wilson E, Piwowar-Manning E, Forman MS, McCauley M, Gamble T, Ruangyuttikarn C, Hosseinipour MC, Kumarasamy N, Nyirenda M, Grinsztejn B, Pilotto JH, Kosashunhanan N, Gonçalves de Melo M, Makhema J, Akelo V, Panchia R, Badal-Faesen S, Chen YQ, Cohen MS, Eshleman SH, Thio CL, Valsamakis A.

J Acquir Immune Defic Syndr. 2017 Dec 1;76(4):388-393. doi: 10.1097/QAI.0000000000001511.


The effect of a genetic variant on quantitative real-time PCR in a case of disseminated adenovirus infection.

Gniadek TJ, Forman MT, Martin I, Arav-Boger R, Valsamakis A.

Diagn Microbiol Infect Dis. 2017 Sep;89(1):40-43. doi: 10.1016/j.diagmicrobio.2017.05.013. Epub 2017 Jun 1.


Chronic Hepatitis B, C, and D.

Cobb BR, Valsamakis A.

Microbiol Spectr. 2016 Aug;4(4). doi: 10.1128/microbiolspec.DMIH2-0025-2015. Review.


Outcomes in Transplant Recipients Treated With Foscarnet for Ganciclovir-Resistant or Refractory Cytomegalovirus Infection.

Avery RK, Arav-Boger R, Marr KA, Kraus E, Shoham S, Lees L, Trollinger B, Shah P, Ambinder R, Neofytos D, Ostrander D, Forman M, Valsamakis A.

Transplantation. 2016 Oct;100(10):e74-80. doi: 10.1097/TP.0000000000001418.


Editorial Commentary: Standardization of Viral Load Testing for Cytomegalovirus: The Long Road Just Got Longer.

Valsamakis A.

Clin Infect Dis. 2016 Sep 1;63(5):590-3. doi: 10.1093/cid/ciw375. Epub 2016 Jun 15. No abstract available.


Hepatitis E Virus Infection Among Solid Organ Transplant Recipients at a North American Transplant Center.

Sue PK, Pisanic N, Heaney CD, Forman M, Valsamakis A, Jackson AM, Ticehurst JR, Montgomery RA, Schwarz KB, Nelson KE, Karnsakul W.

Open Forum Infect Dis. 2016 Jan 18;3(1):ofw006. doi: 10.1093/ofid/ofw006. eCollection 2016 Jan.


The clinical significance of EBV DNA in the plasma and peripheral blood mononuclear cells of patients with or without EBV diseases.

Kanakry JA, Hegde AM, Durand CM, Massie AB, Greer AE, Ambinder RF, Valsamakis A.

Blood. 2016 Apr 21;127(16):2007-17. doi: 10.1182/blood-2015-09-672030. Epub 2016 Jan 7.


A Polymerase Chain Reaction-Based Algorithm to Detect and Prevent Transmission of Adenoviral Conjunctivitis in Hospital Employees.

Kuo IC, Espinosa C, Forman M, Valsamakis A.

Am J Ophthalmol. 2016 Mar;163:38-44. doi: 10.1016/j.ajo.2015.12.007. Epub 2015 Dec 13.


False-positive hepatitis C virus serology after placement of a ventricular assistance device.

Durand CM, Marr KA, Ostrander D, Subramanian A, Valsamakis A, Cox A, Neofytos D.

Transpl Infect Dis. 2016 Feb;18(1):146-9. doi: 10.1111/tid.12483. Epub 2016 Jan 30.


Comparison of BKV quantification using a single automated nucleic acid extraction platform and 3 real-time PCR assays.

Greer AE, Forman MS, Valsamakis A.

Diagn Microbiol Infect Dis. 2015 Aug;82(4):297-302. doi: 10.1016/j.diagmicrobio.2015.04.006. Epub 2015 Apr 25.


Clinical diagnosis of influenza in the ED.

Dugas AF, Valsamakis A, Atreya MR, Thind K, Alarcon Manchego P, Faisal A, Gaydos CA, Rothman RE.

Am J Emerg Med. 2015 Jun;33(6):770-5. doi: 10.1016/j.ajem.2015.03.008. Epub 2015 Mar 12.


Variability of hepatitis E serologic assays in a pediatric liver transplant recipient: challenges to diagnosing hepatitis E virus infection in the United States.

Sue PK, Pisanic N, Heaney CD, Mixson-Hayden T, Kamili S, Nelson K, Schwarz KB, Forman M, Valsamakis A, Ticehurst J, Karnsakul W.

Transpl Infect Dis. 2015 Apr;17(2):284-8. doi: 10.1111/tid.12366.


Evaluation of the Xpert Flu rapid PCR assay in high-risk emergency department patients.

Dugas AF, Valsamakis A, Gaydos CA, Forman M, Hardick J, Kidambi P, Amin S, Gupta A, Rothman RE.

J Clin Microbiol. 2014 Dec;52(12):4353-5. doi: 10.1128/JCM.02343-14. Epub 2014 Sep 24.


Detection and prevalence of adenoviral conjunctivitis among hospital employees using real-time polymerase chain reaction as an infection prevention tool.

Kuo IC, Espinosa C, Forman M, Pehar M, Maragakis LL, Valsamakis A.

Infect Control Hosp Epidemiol. 2014 Jun;35(6):728-31. doi: 10.1086/676428. Epub 2014 Apr 23.


BK virus replication in patients with anti-neutrophil cytoplasmic antibody-associated vasculitis.

Geetha D, Levine SM, Manno RL, Valsamakis A, Ghazarian S, Seo P.

Am J Nephrol. 2014;39(1):20-6. doi: 10.1159/000357409. Epub 2013 Dec 31. Erratum in: Am J Nephrol. 2014;40(1):96.


Case report: congenital rubella syndrome: a rare but persistent concern in the United States.

Fang J, Agrawal A, Gowtham S, Felling RJ, Jalazo E, Park HJ, Valsamakis A, Huisman TA, Golden WC.

J Perinatol. 2013 Nov;33(11):899-902. doi: 10.1038/jp.2013.73.


Multicenter evaluation of a commercial cytomegalovirus quantitative standard: effects of commutability on interlaboratory concordance.

Hayden RT, Shahbazian MD, Valsamakis A, Boonyaratanakornkit J, Cook L, Pang XL, Preiksaitis JK, Schönbrunner ER, Caliendo AM.

J Clin Microbiol. 2013 Nov;51(11):3811-7. doi: 10.1128/JCM.02036-13. Epub 2013 Sep 11.


Detection of cytomegalovirus DNA in plasma as an adjunct diagnostic for gastrointestinal tract disease in kidney and liver transplant recipients.

Durand CM, Marr KA, Arnold CA, Tang L, Durand DJ, Avery RK, Valsamakis A, Neofytos D.

Clin Infect Dis. 2013 Dec;57(11):1550-9. doi: 10.1093/cid/cit521. Epub 2013 Aug 15.


Derivation of a clinical prediction rule to predict hospitalization for influenza in EDs.

Chen KF, Hsieh YH, Gaydos CA, Valsamakis A, Rothman RE.

Am J Emerg Med. 2013 Mar;31(3):529-34. doi: 10.1016/j.ajem.2012.10.010. Epub 2013 Feb 4.


An international multicenter performance analysis of cytomegalovirus load tests.

Hirsch HH, Lautenschlager I, Pinsky BA, Cardeñoso L, Aslam S, Cobb B, Vilchez RA, Valsamakis A.

Clin Infect Dis. 2013 Feb;56(3):367-73. doi: 10.1093/cid/cis900. Epub 2012 Oct 24.


Clinical accuracy of a PLEX-ID flu device for simultaneous detection and identification of influenza viruses A and B.

Tang YW, Lowery KS, Valsamakis A, Schaefer VC, Chappell JD, White-Abell J, Quinn CD, Li H, Washington CA, Cromwell J, Giamanco CM, Forman M, Holden J, Rothman RE, Parker ML, Ortenberg EV, Zhang L, Lin YL, Gaydos CA.

J Clin Microbiol. 2013 Jan;51(1):40-5. doi: 10.1128/JCM.01978-12. Epub 2012 Oct 17.


Diagnostic performance of two highly multiplexed respiratory virus assays in a pediatric cohort.

Forman MS, Advani S, Newman C, Gaydos CA, Milstone AM, Valsamakis A.

J Clin Virol. 2012 Oct;55(2):168-72. doi: 10.1016/j.jcv.2012.06.019. Epub 2012 Jul 24.


Detecting respiratory viruses in asymptomatic children.

Advani S, Sengupta A, Forman M, Valsamakis A, Milstone AM.

Pediatr Infect Dis J. 2012 Dec;31(12):1221-6. doi: 10.1097/INF.0b013e318265a804.


Dried urine spots for detection and quantification of cytomegalovirus in newborns.

Forman M, Valsamakis A, Arav-Boger R.

Diagn Microbiol Infect Dis. 2012 Aug;73(4):326-9. doi: 10.1016/j.diagmicrobio.2012.04.009. Epub 2012 Jun 1.


An outbreak of human parainfluenza virus 3 infection in an outpatient hematopoietic stem cell transplantation clinic.

Sydnor ER, Greer A, Budd AP, Pehar M, Munshaw S, Neofytos D, Perl TM, Valsamakis A.

Am J Infect Control. 2012 Sep;40(7):601-5. doi: 10.1016/j.ajic.2011.11.011. Epub 2012 Mar 8.


Epidemiology of respiratory viruses in children admitted to an infant/toddler unit.

Milstone AM, Perl TM, Valsamakis A.

Am J Infect Control. 2012 Jun;40(5):462-4. doi: 10.1016/j.ajic.2011.05.024. Epub 2011 Sep 9.


Application of the hybrid capture 2 assay to squamous cell carcinomas of the head and neck: a convenient liquid-phase approach for the reliable determination of human papillomavirus status.

Bishop JA, Maleki Z, Valsamakis A, Ogawa T, Chang X, Pai SI, Westra WH.

Cancer Cytopathol. 2012 Feb 25;120(1):18-25. doi: 10.1002/cncy.20175. Epub 2011 Jul 12.


Multilaboratory evaluation of real-time PCR tests for hepatitis B virus DNA quantification.

Caliendo AM, Valsamakis A, Bremer JW, Ferreira-Gonzalez A, Granger S, Sabatini L, Tsongalis GJ, Wang YF, Yen-Lieberman B, Young S, Lurain NS.

J Clin Microbiol. 2011 Aug;49(8):2854-8. doi: 10.1128/JCM.00471-11. Epub 2011 Jun 22.


Cytomegalovirus DNA quantification using an automated platform for nucleic acid extraction and real-time PCR assay setup.

Forman M, Wilson A, Valsamakis A.

J Clin Microbiol. 2011 Jul;49(7):2703-5. doi: 10.1128/JCM.00721-11. Epub 2011 May 11.


Rapid identification viruses from nasal pharyngeal aspirates in acute viral respiratory infections by RT-PCR and electrospray ionization mass spectrometry.

Chen KF, Rothman RE, Ramachandran P, Blyn L, Sampath R, Ecker DJ, Valsamakis A, Gaydos CA.

J Virol Methods. 2011 Apr;173(1):60-6. doi: 10.1016/j.jviromet.2011.01.007. Epub 2011 Jan 21.


Reverse transcription polymerase chain reaction and electrospray ionization mass spectrometry for identifying acute viral upper respiratory tract infections.

Chen KF, Blyn L, Rothman RE, Ramachandran P, Valsamakis A, Ecker D, Sampath R, Gaydos CA.

Diagn Microbiol Infect Dis. 2011 Feb;69(2):179-86. doi: 10.1016/j.diagmicrobio.2010.10.010.


The combination of ribavirin and peginterferon is superior to peginterferon and placebo for children and adolescents with chronic hepatitis C.

Schwarz KB, Gonzalez-Peralta RP, Murray KF, Molleston JP, Haber BA, Jonas MM, Rosenthal P, Mohan P, Balistreri WF, Narkewicz MR, Smith L, Lobritto SJ, Rossi S, Valsamakis A, Goodman Z, Robuck PR, Barton BA; Peds-C Clinical Research Network.

Gastroenterology. 2011 Feb;140(2):450-458.e1. doi: 10.1053/j.gastro.2010.10.047. Epub 2010 Oct 28.


Clinical outcomes of seasonal influenza and pandemic influenza A (H1N1) in pediatric inpatients.

Tamma PD, Turnbull AE, Milstone AM, Cosgrove SE, Valsamakis A, Budd A, Perl TM.

BMC Pediatr. 2010 Oct 6;10:72. doi: 10.1186/1471-2431-10-72.


Severe pandemic H1N1 and seasonal influenza in children and young adults with sickle cell disease.

Strouse JJ, Reller ME, Bundy DG, Amoako M, Cancio M, Han RN, Valsamakis A, Casella JF.

Blood. 2010 Nov 4;116(18):3431-4. doi: 10.1182/blood-2010-05-282194. Epub 2010 Jul 23.


Multicenter study of clinical performance of the 3M Rapid Detection RSV test.

Ginocchio CC, Swierkosz E, McAdam AJ, Marcon M, Storch GA, Valsamakis A, Juretschko S, Romero J, Yen-Lieberman B.

J Clin Microbiol. 2010 Jul;48(7):2337-43. doi: 10.1128/JCM.00130-10. Epub 2010 May 12.


The critically ill child with novel H1N1 influenza A: a case series.

Lockman JL, Fischer WA, Perl TM, Valsamakis A, Nichols DG.

Pediatr Crit Care Med. 2010 Mar;11(2):173-8. doi: 10.1097/PCC.0b013e3181ccedae.


A clinical algorithm identifies high risk pediatric oncology and bone marrow transplant patients likely to benefit from treatment of adenoviral infection.

Williams KM, Agwu AL, Dabb AA, Higman MA, Loeb DM, Valsamakis A, Chen AR.

J Pediatr Hematol Oncol. 2009 Nov;31(11):825-31. doi: 10.1097/MPH.0b013e3181b7873e.


Evaluation of an automated, highly sensitive, real-time PCR-based assay (COBAS Ampliprep/COBAS TaqMan) for quantification of HCV RNA.

Sarrazin C, Dragan A, Gärtner BC, Forman MS, Traver S, Zeuzem S, Valsamakis A.

J Clin Virol. 2008 Oct;43(2):162-8. doi: 10.1016/j.jcv.2008.06.013. Epub 2008 Aug 8.


Differential CMV-specific CD8+ effector T cell responses in the lung allograft predominate over the blood during human primary infection.

Pipeling MR, West EE, Osborne CM, Whitlock AB, Dropulic LK, Willett MH, Forman M, Valsamakis A, Orens JB, Moller DR, Lechtzin N, Migueles SA, Connors M, McDyer JF.

J Immunol. 2008 Jul 1;181(1):546-56.


Multicenter comparison of different real-time PCR assays for quantitative detection of Epstein-Barr virus.

Hayden RT, Hokanson KM, Pounds SB, Bankowski MJ, Belzer SW, Carr J, Diorio D, Forman MS, Joshi Y, Hillyard D, Hodinka RL, Nikiforova MN, Romain CA, Stevenson J, Valsamakis A, Balfour HH Jr; U.S. EBV Working Group.

J Clin Microbiol. 2008 Jan;46(1):157-63. Epub 2007 Nov 7.


Molecular testing in the diagnosis and management of chronic hepatitis B.

Valsamakis A.

Clin Microbiol Rev. 2007 Jul;20(3):426-39, table of contents. Review.


Multilaboratory comparison of hepatitis C virus viral load assays.

Caliendo AM, Valsamakis A, Zhou Y, Yen-Lieberman B, Andersen J, Young S, Ferreira-Gonzalez A, Tsongalis GJ, Pyles R, Bremer JW, Lurain NS.

J Clin Microbiol. 2006 May;44(5):1726-32.


Respiratory syncytial virus detection by Remel Xpect, Binax Now RSV, direct immunofluorescent staining, and tissue culture.

Borek AP, Clemens SH, Gaskins VK, Aird DZ, Valsamakis A.

J Clin Microbiol. 2006 Mar;44(3):1105-7.


Low prevalence of pertussis among children admitted with respiratory symptoms during respiratory syncytial virus season.

Siberry GK, Paquette NR, Ross TL, Perl TM, Valsamakis A.

Infect Control Hosp Epidemiol. 2006 Jan;27(1):95-7. Epub 2006 Jan 6.


Modulation of disease, T cell responses, and measles virus clearance in monkeys vaccinated with H-encoding alphavirus replicon particles.

Pan CH, Valsamakis A, Colella T, Nair N, Adams RJ, Polack FP, Greer CE, Perri S, Polo JM, Griffin DE.

Proc Natl Acad Sci U S A. 2005 Aug 16;102(33):11581-8. Epub 2005 Jul 21.

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