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Special Issue: Recombinant Protein Expression in Microorganisms.

Brautaset T, Valla S.

Microorganisms. 2019 Sep 16;7(9). pii: E355. doi: 10.3390/microorganisms7090355.


RpoS controls the expression and the transport of the AlgE1-7 epimerases in Azotobacter vinelandii.

Moreno S, Ertesvåg H, Valla S, Núñez C, Espin G, Cocotl-Yañez M.

FEMS Microbiol Lett. 2018 Oct 1;365(19). doi: 10.1093/femsle/fny210.


The XylS/Pm regulator/promoter system and its use in fundamental studies of bacterial gene expression, recombinant protein production and metabolic engineering.

Gawin A, Valla S, Brautaset T.

Microb Biotechnol. 2017 Jul;10(4):702-718. doi: 10.1111/1751-7915.12701. Epub 2017 Mar 9. Review.


Identification of genes affecting alginate biosynthesis in Pseudomonas fluorescens by screening a transposon insertion library.

Ertesvåg H, Sletta H, Senneset M, Sun YQ, Klinkenberg G, Konradsen TA, Ellingsen TE, Valla S.

BMC Genomics. 2017 Jan 3;18(1):11. doi: 10.1186/s12864-016-3467-7.


New insights into Pseudomonas fluorescens alginate biosynthesis relevant for the establishment of an efficient production process for microbial alginates.

Maleki S, Mærk M, Hrudikova R, Valla S, Ertesvåg H.

N Biotechnol. 2017 Jul 25;37(Pt A):2-8. doi: 10.1016/j.nbt.2016.08.005. Epub 2016 Sep 1. Review.


Alginate Biosynthesis Factories in Pseudomonas fluorescens: Localization and Correlation with Alginate Production Level.

Maleki S, Almaas E, Zotchev S, Valla S, Ertesvåg H.

Appl Environ Microbiol. 2015 Dec 11;82(4):1227-1236. doi: 10.1128/AEM.03114-15. Print 2016 Feb 15.


Benzoic Acid-Inducible Gene Expression in Mycobacteria.

Dragset MS, Barczak AK, Kannan N, Mærk M, Flo TH, Valla S, Rubin EJ, Steigedal M.

PLoS One. 2015 Sep 8;10(9):e0134544. doi: 10.1371/journal.pone.0134544. eCollection 2015.


Mutational Analyses of Glucose Dehydrogenase and Glucose-6-Phosphate Dehydrogenase Genes in Pseudomonas fluorescens Reveal Their Effects on Growth and Alginate Production.

Maleki S, Mærk M, Valla S, Ertesvåg H.

Appl Environ Microbiol. 2015 May 15;81(10):3349-56. doi: 10.1128/AEM.03653-14. Epub 2015 Mar 6.


Structural and functional characterization of the R-modules in alginate C-5 epimerases AlgE4 and AlgE6 from Azotobacter vinelandii.

Buchinger E, Knudsen DH, Behrens MA, Pedersen JS, Aarstad OA, Tøndervik A, Valla S, Skjåk-Bræk G, Wimmer R, Aachmann FL.

J Biol Chem. 2014 Nov 7;289(45):31382-96. doi: 10.1074/jbc.M114.567008. Epub 2014 Sep 29.


Safety in numbers: multiple occurrences of highly similar homologs among Azotobacter vinelandii carbohydrate metabolism proteins probably confer adaptive benefits.

Mærk M, Johansen J, Ertesvåg H, Drabløs F, Valla S.

BMC Genomics. 2014 Mar 14;15:192. doi: 10.1186/1471-2164-15-192.


A new and improved host-independent plasmid system for RK2-based conjugal transfer.

Strand TA, Lale R, Degnes KF, Lando M, Valla S.

PLoS One. 2014 Mar 3;9(3):e90372. doi: 10.1371/journal.pone.0090372. eCollection 2014.


Design and optimization of short DNA sequences that can be used as 5' fusion partners for high-level expression of heterologous genes in Escherichia coli.

Kucharova V, Skancke J, Brautaset T, Valla S.

Appl Environ Microbiol. 2013 Nov;79(21):6655-64. doi: 10.1128/AEM.01676-13. Epub 2013 Aug 23.


Meeting report: 1st international functional metagenomics workshop may 7-8, 2012, st. Jacobs, ontario, Canada.

Engel K, Ashby D, Brady SF, Cowan DA, Doemer J, Edwards EA, Fiebig K, Martens EC, McCormac D, Mead DA, Miyazaki K, Moreno-Hagelsieb G, O'Gara F, Reid A, Rose DR, Simonet P, Sjöling S, Smalla K, Streit WR, Tedman-Jones J, Valla S, Wellington EM, Wu CC, Liles MR, Neufeld JD, Sessitsch A, Charles TC.

Stand Genomic Sci. 2013 Apr 15;8(1):106-11. doi: 10.4056/sigs.3406845. eCollection 2013 Apr 15.


Combinatorial mutagenesis and selection to understand and improve yeast promoters.

Berg L, Strand TA, Valla S, Brautaset T.

Biomed Res Int. 2013;2013:926985. doi: 10.1155/2013/926985. Epub 2013 Jun 6.


Non-Invasive Analysis of Recombinant mRNA Stability in Escherichia coli by a Combination of Transcriptional Inducer Wash-Out and qRT-PCR.

Kucharova V, Strand TA, Almaas E, Naas AE, Brautaset T, Valla S.

PLoS One. 2013 Jun 19;8(6):e66429. doi: 10.1371/journal.pone.0066429. Print 2013.


The microbial communities in two apparently physically separated deep subsurface oil reservoirs show extensive DNA sequence similarities.

Lewin A, Johansen J, Wentzel A, Kotlar HK, Drabløs F, Valla S.

Environ Microbiol. 2014 Feb;16(2):545-58. doi: 10.1111/1462-2920.12181. Epub 2013 Jul 4.


Mannuronan C-5 epimerases suited for tailoring of specific alginate structures obtained by high-throughput screening of an epimerase mutant library.

Tøndervik A, Klinkenberg G, Aachmann FL, Svanem BI, Ertesvåg H, Ellingsen TE, Valla S, Skjåk-Bræk G, Sletta H.

Biomacromolecules. 2013 Aug 12;14(8):2657-66. doi: 10.1021/bm4005194. Epub 2013 Jun 28.


Combinatorial engineering for heterologous gene expression.

Zwick F, Lale R, Valla S.

Bioengineered. 2013 Nov-Dec;4(6):431-4. doi: 10.4161/bioe.24703. Epub 2013 Apr 22.


A comparative analysis of the properties of regulated promoter systems commonly used for recombinant gene expression in Escherichia coli.

Balzer S, Kucharova V, Megerle J, Lale R, Brautaset T, Valla S.

Microb Cell Fact. 2013 Mar 18;12:26. doi: 10.1186/1475-2859-12-26.


Mapping global effects of the anti-sigma factor MucA in Pseudomonas fluorescens SBW25 through genome-scale metabolic modeling.

Borgos SE, Bordel S, Sletta H, Ertesvåg H, Jakobsen Ø, Bruheim P, Ellingsen TE, Nielsen J, Valla S.

BMC Syst Biol. 2013 Mar 11;7:19. doi: 10.1186/1752-0509-7-19.


Metagenomics of microbial life in extreme temperature environments.

Lewin A, Wentzel A, Valla S.

Curr Opin Biotechnol. 2013 Jun;24(3):516-25. doi: 10.1016/j.copbio.2012.10.012. Epub 2012 Nov 9. Review.


Combinatorial mutagenesis and selection of improved signal sequences and their application for high-level production of translocated heterologous proteins in Escherichia coli.

Heggeset TM, Kucharova V, Naerdal I, Valla S, Sletta H, Ellingsen TE, Brautaset T.

Appl Environ Microbiol. 2013 Jan;79(2):559-68. doi: 10.1128/AEM.02407-12. Epub 2012 Nov 9.


Rapid reagentless quantification of alginate biosynthesis in Pseudomonas fluorescens bacteria mutants using FT-IR spectroscopy coupled to multivariate partial least squares regression.

Correa E, Sletta H, Ellis DI, Hoel S, Ertesvåg H, Ellingsen TE, Valla S, Goodacre R.

Anal Bioanal Chem. 2012 Jul;403(9):2591-9. doi: 10.1007/s00216-012-6068-6. Epub 2012 May 15.


High coverage sequencing of DNA from microorganisms living in an oil reservoir 2.5 kilometres subsurface.

Kotlar HK, Lewin A, Johansen J, Throne-Holst M, Haverkamp T, Markussen S, Winnberg A, Ringrose P, Aakvik T, Ryeng E, Jakobsen K, Drabløs F, Valla S.

Environ Microbiol Rep. 2011 Dec;3(6):674-81. doi: 10.1111/j.1758-2229.2011.00279.x. Epub 2011 Sep 14.


Ralstonia sp. U2 naphthalene dioxygenase and Comamonas sp. JS765 nitrobenzene dioxygenase show differences in activity towards methylated naphthalenes.

Tøndervik A, Bruheim P, Berg L, Ellingsen TE, Kotlar HK, Valla S, Throne-Holst M.

J Biosci Bioeng. 2012 Feb;113(2):173-8. doi: 10.1016/j.jbiosc.2011.10.001. Epub 2011 Oct 28.


Broad-host-range plasmid vectors for gene expression in bacteria.

Lale R, Brautaset T, Valla S.

Methods Mol Biol. 2011;765:327-43. doi: 10.1007/978-1-61779-197-0_19.


Exploring the 5'-UTR DNA region as a target for optimizing recombinant gene expression from the strong and inducible Pm promoter in Escherichia coli.

Berg L, Kucharova V, Bakke I, Valla S, Brautaset T.

J Biotechnol. 2012 Apr 30;158(4):224-30. doi: 10.1016/j.jbiotec.2011.07.012. Epub 2011 Jul 27.


Continuous control of the flow in biochemical pathways through 5' untranslated region sequence modifications in mRNA expressed from the broad-host-range promoter Pm.

Lale R, Berg L, Stüttgen F, Netzer R, Stafsnes M, Brautaset T, Vee Aune TE, Valla S.

Appl Environ Microbiol. 2011 Apr;77(8):2648-55. doi: 10.1128/AEM.02091-10. Epub 2011 Feb 18.


Directed evolution of the transcription factor XylS for development of improved expression systems.

Vee Aune TE, Bakke I, Drabløs F, Lale R, Brautaset T, Valla S.

Microb Biotechnol. 2010 Jan;3(1):38-47. doi: 10.1111/j.1751-7915.2009.00126.x. Epub 2009 Jun 3.


1H, 13C and 15N resonances of the AlgE62 subunit from Azotobacter vinelandii mannuronan C5-epimerase.

Andreassen T, Buchinger E, Skjåk-Bræk G, Valla S, Aachmann FL.

Biomol NMR Assign. 2011 Oct;5(2):147-9. doi: 10.1007/s12104-010-9288-8. Epub 2010 Dec 28.


Isolation of mutant alginate lyases with cleavage specificity for di-guluronic acid linkages.

Tøndervik A, Klinkenberg G, Aarstad OA, Drabløs F, Ertesvåg H, Ellingsen TE, Skjåk-Bræk G, Valla S, Sletta H.

J Biol Chem. 2010 Nov 12;285(46):35284-92. doi: 10.1074/jbc.M110.162800. Epub 2010 Sep 7.


NMR assignments of 1H, 13C and 15N resonances of the C-terminal subunit from Azotobacter vinelandii mannuronan C5-epimerase 6 (AlgE6R3).

Buchinger E, Skjåk-Bræk G, Valla S, Wimmer R, Aachmann FL.

Biomol NMR Assign. 2011 Apr;5(1):27-9. doi: 10.1007/s12104-010-9259-0. Epub 2010 Aug 14.


Use of protein trans-splicing to produce active and segmentally (2)H, (15)N labeled mannuronan C5-epimerase AlgE4.

Buchinger E, Aachmann FL, Aranko AS, Valla S, Skjåk-Braek G, Iwaï H, Wimmer R.

Protein Sci. 2010 Aug;19(8):1534-43. doi: 10.1002/pro.432.


Isolation and characterization of marine pigmented bacteria from Norwegian coastal waters and screening for carotenoids with UVA-blue light absorbing properties.

Stafsnes MH, Josefsen KD, Kildahl-Andersen G, Valla S, Ellingsen TE, Bruheim P.

J Microbiol. 2010 Feb;48(1):16-23. doi: 10.1007/s12275-009-0118-6. Epub 2010 Mar 11.


Characterization of three new Azotobacter vinelandii alginate lyases, one of which is involved in cyst germination.

Gimmestad M, Ertesvåg H, Heggeset TM, Aarstad O, Svanem BI, Valla S.

J Bacteriol. 2009 Aug;191(15):4845-53. doi: 10.1128/JB.00455-09. Epub 2009 May 29.


A plasmid RK2-based broad-host-range cloning vector useful for transfer of metagenomic libraries to a variety of bacterial species.

Aakvik T, Degnes KF, Dahlsrud R, Schmidt F, Dam R, Yu L, Völker U, Ellingsen TE, Valla S.

FEMS Microbiol Lett. 2009 Jun;296(2):149-58. doi: 10.1111/j.1574-6968.2009.01639.x. Epub 2009 May 4.


Genome sequence of Azotobacter vinelandii, an obligate aerobe specialized to support diverse anaerobic metabolic processes.

Setubal JC, dos Santos P, Goldman BS, Ertesvåg H, Espin G, Rubio LM, Valla S, Almeida NF, Balasubramanian D, Cromes L, Curatti L, Du Z, Godsy E, Goodner B, Hellner-Burris K, Hernandez JA, Houmiel K, Imperial J, Kennedy C, Larson TJ, Latreille P, Ligon LS, Lu J, Maerk M, Miller NM, Norton S, O'Carroll IP, Paulsen I, Raulfs EC, Roemer R, Rosser J, Segura D, Slater S, Stricklin SL, Studholme DJ, Sun J, Viana CJ, Wallin E, Wang B, Wheeler C, Zhu H, Dean DR, Dixon R, Wood D.

J Bacteriol. 2009 Jul;191(14):4534-45. doi: 10.1128/JB.00504-09. Epub 2009 May 8.


The future is artificial.

Valla S.

Microb Biotechnol. 2009 Mar;2(2):152-3. doi: 10.1111/j.1751-7915.2009.00090_19.x. No abstract available.


Random mutagenesis of the PM promoter as a powerful strategy for improvement of recombinant-gene expression.

Bakke I, Berg L, Aune TE, Brautaset T, Sletta H, Tøndervik A, Valla S.

Appl Environ Microbiol. 2009 Apr;75(7):2002-11. doi: 10.1128/AEM.02315-08. Epub 2009 Feb 5.


Positively regulated bacterial expression systems.

Brautaset T, Lale R, Valla S.

Microb Biotechnol. 2009 Jan;2(1):15-30. doi: 10.1111/j.1751-7915.2008.00048.x. Epub 2008 Oct 15. Review.


Overexpression of wild-type aspartokinase increases L-lysine production in the thermotolerant methylotrophic bacterium Bacillus methanolicus.

Jakobsen OM, Brautaset T, Degnes KF, Heggeset TM, Balzer S, Flickinger MC, Valla S, Ellingsen TE.

Appl Environ Microbiol. 2009 Feb;75(3):652-61. doi: 10.1128/AEM.01176-08. Epub 2008 Dec 5.


Structural and mutational characterization of the catalytic A-module of the mannuronan C-5-epimerase AlgE4 from Azotobacter vinelandii.

Rozeboom HJ, Bjerkan TM, Kalk KH, Ertesvåg H, Holtan S, Aachmann FL, Valla S, Dijkstra BW.

J Biol Chem. 2008 Aug 29;283(35):23819-28. doi: 10.1074/jbc.M804119200. Epub 2008 Jun 23.


The Azotobacter vinelandii AlgE mannuronan C-5-epimerase family is essential for the in vivo control of alginate monomer composition and for functional cyst formation.

Steigedal M, Sletta H, Moreno S, Maerk M, Christensen BE, Bjerkan T, Ellingsen TE, Espìn G, Ertesvåg H, Valla S.

Environ Microbiol. 2008 Jul;10(7):1760-70. doi: 10.1111/j.1462-2920.2008.01597.x. Epub 2008 Mar 28.


The presence of N-terminal secretion signal sequences leads to strong stimulation of the total expression levels of three tested medically important proteins during high-cell-density cultivations of Escherichia coli.

Sletta H, Tøndervik A, Hakvåg S, Aune TE, Nedal A, Aune R, Evensen G, Valla S, Ellingsen TE, Brautaset T.

Appl Environ Microbiol. 2007 Feb;73(3):906-12. Epub 2006 Dec 1.


Identification and characterization of an Azotobacter vinelandii type I secretion system responsible for export of the AlgE-type mannuronan C-5-epimerases.

Gimmestad M, Steigedal M, Ertesvåg H, Moreno S, Christensen BE, Espín G, Valla S.

J Bacteriol. 2006 Aug;188(15):5551-60.

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