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Items: 23

1.

Overexpression and purification of human myosins from transiently and stably transfected suspension adapted HEK293SF-3F6 cells.

Ušaj M, Zattelman L, Regev R, Shneyer BI, Wiesel-Motiuk N, Henn A.

Anal Biochem. 2018 Oct 1;558:19-27. doi: 10.1016/j.ab.2018.07.026. Epub 2018 Aug 1.

PMID:
30075102
2.

Blebbistatin Effects Expose Hidden Secrets in the Force-Generating Cycle of Actin and Myosin.

Rahman MA, Ušaj M, Rassier DE, Månsson A.

Biophys J. 2018 Jul 17;115(2):386-397. doi: 10.1016/j.bpj.2018.05.037.

3.

Do Actomyosin Single-Molecule Mechanics Data Predict Mechanics of Contracting Muscle?

Månsson A, Ušaj M, Moretto L, Rassier DE.

Int J Mol Sci. 2018 Jun 25;19(7). pii: E1863. doi: 10.3390/ijms19071863. Review.

4.

Systematic analysis of complex genetic interactions.

Kuzmin E, VanderSluis B, Wang W, Tan G, Deshpande R, Chen Y, Usaj M, Balint A, Mattiazzi Usaj M, van Leeuwen J, Koch EN, Pons C, Dagilis AJ, Pryszlak M, Wang JZY, Hanchard J, Riggi M, Xu K, Heydari H, San Luis BJ, Shuteriqi E, Zhu H, Van Dyk N, Sharifpoor S, Costanzo M, Loewith R, Caudy A, Bolnick D, Brown GW, Andrews BJ, Boone C, Myers CL.

Science. 2018 Apr 20;360(6386). pii: eaao1729. doi: 10.1126/science.aao1729.

5.

Kinetic adaptation of human Myo19 for active mitochondrial transport to growing filopodia tips.

Ušaj M, Henn A.

Sci Rep. 2017 Sep 14;7(1):11596. doi: 10.1038/s41598-017-11984-6.

6.

ROS induced distribution of mitochondria to filopodia by Myo19 depends on a class specific tryptophan in the motor domain.

Shneyer BI, Ušaj M, Wiesel-Motiuk N, Regev R, Henn A.

Sci Rep. 2017 Sep 14;7(1):11577. doi: 10.1038/s41598-017-11002-9.

7.

N-terminal splicing extensions of the human MYO1C gene fine-tune the kinetics of the three full-length myosin IC isoforms.

Zattelman L, Regev R, Ušaj M, Reinke PYA, Giese S, Samson AO, Taft MH, Manstein DJ, Henn A.

J Biol Chem. 2017 Oct 27;292(43):17804-17818. doi: 10.1074/jbc.M117.794008. Epub 2017 Sep 11.

8.

Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens.

Hart T, Tong AHY, Chan K, Van Leeuwen J, Seetharaman A, Aregger M, Chandrashekhar M, Hustedt N, Seth S, Noonan A, Habsid A, Sizova O, Nedyalkova L, Climie R, Tworzyanski L, Lawson K, Sartori MA, Alibeh S, Tieu D, Masud S, Mero P, Weiss A, Brown KR, Usaj M, Billmann M, Rahman M, Constanzo M, Myers CL, Andrews BJ, Boone C, Durocher D, Moffat J.

G3 (Bethesda). 2017 Aug 7;7(8):2719-2727. doi: 10.1534/g3.117.041277.

9.

TheCellMap.org: A Web-Accessible Database for Visualizing and Mining the Global Yeast Genetic Interaction Network.

Usaj M, Tan Y, Wang W, VanderSluis B, Zou A, Myers CL, Costanzo M, Andrews B, Boone C.

G3 (Bethesda). 2017 May 5;7(5):1539-1549. doi: 10.1534/g3.117.040220.

10.

Exploring genetic suppression interactions on a global scale.

van Leeuwen J, Pons C, Mellor JC, Yamaguchi TN, Friesen H, Koschwanez J, Ušaj MM, Pechlaner M, Takar M, Ušaj M, VanderSluis B, Andrusiak K, Bansal P, Baryshnikova A, Boone CE, Cao J, Cote A, Gebbia M, Horecka G, Horecka I, Kuzmin E, Legro N, Liang W, van Lieshout N, McNee M, San Luis BJ, Shaeri F, Shuteriqi E, Sun S, Yang L, Youn JY, Yuen M, Costanzo M, Gingras AC, Aloy P, Oostenbrink C, Murray A, Graham TR, Myers CL, Andrews BJ, Roth FP, Boone C.

Science. 2016 Nov 4;354(6312). pii: aag0839.

11.

A global genetic interaction network maps a wiring diagram of cellular function.

Costanzo M, VanderSluis B, Koch EN, Baryshnikova A, Pons C, Tan G, Wang W, Usaj M, Hanchard J, Lee SD, Pelechano V, Styles EB, Billmann M, van Leeuwen J, van Dyk N, Lin ZY, Kuzmin E, Nelson J, Piotrowski JS, Srikumar T, Bahr S, Chen Y, Deshpande R, Kurat CF, Li SC, Li Z, Usaj MM, Okada H, Pascoe N, San Luis BJ, Sharifpoor S, Shuteriqi E, Simpkins SW, Snider J, Suresh HG, Tan Y, Zhu H, Malod-Dognin N, Janjic V, Przulj N, Troyanskaya OG, Stagljar I, Xia T, Ohya Y, Gingras AC, Raught B, Boutros M, Steinmetz LM, Moore CL, Rosebrock AP, Caudy AA, Myers CL, Andrews B, Boone C.

Science. 2016 Sep 23;353(6306). pii: aaf1420.

12.

Exploring Quantitative Yeast Phenomics with Single-Cell Analysis of DNA Damage Foci.

Styles EB, Founk KJ, Zamparo LA, Sing TL, Altintas D, Ribeyre C, Ribaud V, Rougemont J, Mayhew D, Costanzo M, Usaj M, Verster AJ, Koch EN, Novarina D, Graf M, Luke B, Muzi-Falconi M, Myers CL, Mitra RD, Shore D, Brown GW, Zhang Z, Boone C, Andrews BJ.

Cell Syst. 2016 Sep 28;3(3):264-277.e10. doi: 10.1016/j.cels.2016.08.008. Epub 2016 Sep 8.

13.

Myo19 is an outer mitochondrial membrane motor and effector of starvation-induced filopodia.

Shneyer BI, Ušaj M, Henn A.

J Cell Sci. 2016 Feb 1;129(3):543-56. doi: 10.1242/jcs.175349. Epub 2015 Dec 11.

14.

Modified Adherence Method (MAM) for Electrofusion of Anchorage-Dependent Cells.

Ušaj M, Kandušer M.

Methods Mol Biol. 2015;1313:203-16. doi: 10.1007/978-1-4939-2703-6_15.

PMID:
25947667
15.

RNA-dependent chromatin localization of KDM4D lysine demethylase promotes H3K9me3 demethylation.

Zoabi M, Nadar-Ponniah PT, Khoury-Haddad H, Usaj M, Budowski-Tal I, Haran T, Henn A, Mandel-Gutfreund Y, Ayoub N.

Nucleic Acids Res. 2014 Dec 1;42(21):13026-38. doi: 10.1093/nar/gku1021. Epub 2014 Nov 5.

16.

Cell electrofusion: past and future perspectives for antibody production and cancer cell vaccines.

Kandušer M, Ušaj M.

Expert Opin Drug Deliv. 2014 Dec;11(12):1885-98. doi: 10.1517/17425247.2014.938632. Epub 2014 Jul 10. Review.

PMID:
25010248
17.

Cell electrofusion using nanosecond electric pulses.

Rems L, Ušaj M, Kandušer M, Reberšek M, Miklavčič D, Pucihar G.

Sci Rep. 2013 Nov 29;3:3382. doi: 10.1038/srep03382.

18.

SGAtools: one-stop analysis and visualization of array-based genetic interaction screens.

Wagih O, Usaj M, Baryshnikova A, VanderSluis B, Kuzmin E, Costanzo M, Myers CL, Andrews BJ, Boone CM, Parts L.

Nucleic Acids Res. 2013 Jul;41(Web Server issue):W591-6. doi: 10.1093/nar/gkt400. Epub 2013 May 15.

19.

Electrofusion of B16-F1 and CHO cells: the comparison of the pulse first and contact first protocols.

Usaj M, Flisar K, Miklavcic D, Kanduser M.

Bioelectrochemistry. 2013 Feb;89:34-41. doi: 10.1016/j.bioelechem.2012.09.001. Epub 2012 Sep 13. Erratum in: Bioelectrochemistry. 2013 Aug;92:47.

PMID:
23032299
20.

The systematic study of the electroporation and electrofusion of B16-F1 and CHO cells in isotonic and hypotonic buffer.

Usaj M, Kanduser M.

J Membr Biol. 2012 Sep;245(9):583-90. doi: 10.1007/s00232-012-9470-2. Epub 2012 Jul 29.

PMID:
22843161
21.

Cell counting tool parameters optimization approach for electroporation efficiency determination of attached cells in phase contrast images.

Usaj M, Torkar D, Kanduser M, Miklavcic D.

J Microsc. 2011 Mar;241(3):303-14. doi: 10.1111/j.1365-2818.2010.03441.x. Epub 2010 Sep 9.

22.

Cell electrofusion visualized with fluorescence microscopy.

Trontelj K, Usaj M, Miklavcic D.

J Vis Exp. 2010 Jul 1;(41). pii: 1991. doi: 10.3791/1991.

23.

Cell-cell electrofusion: optimization of electric field amplitude and hypotonic treatment for mouse melanoma (B16-F1) and Chinese Hamster ovary (CHO) cells.

Usaj M, Trontelj K, Miklavcic D, Kanduser M.

J Membr Biol. 2010 Jul;236(1):107-16. doi: 10.1007/s00232-010-9272-3. Epub 2010 Jul 14.

PMID:
20628737

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