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Biol Open. 2018 Nov 2;7(11). pii: bio033001. doi: 10.1242/bio.033001.

Drosophila Ror is a nervous system-specific co-receptor for Wnt ligands.

Author information

1
Stem Cell Biology, Institute for Anatomy and Cell Biology, Georg-August University Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany.
2
Laboratory of Developmental Neurobiology, Department of Molecular Cell Biology, Leiden University Medical Center, Einthovenweg 20, 2300RC Leiden, The Netherlands.
3
Stem Cell Biology, Institute for Anatomy and Cell Biology, Georg-August University Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany Karen.Linnemannstoens@zentr.uni-goettingen.de andreas.wodarz@uk-koeln.de.
4
Molecular Cell Biology, Institute I for Anatomy, University of Cologne Medical School, Kerpener Str. 62, 50937 Köln, Germany.
5
Department of Neurobiology, University of Massachusetts Medical School, 364 Plantation Street, LRB 760, Worcester, MA 01605, USA.
6
Cluster of Excellence - Cellular stress response in aging-associated diseases (CECAD), University of Cologne, Joseph-Stelzmann-Str. 26, 50931 Cologne, Germany.

Abstract

Wnt ligands are secreted glycoproteins that control many developmental processes and are crucial for homeostasis of numerous tissues in the adult organism. Signal transduction of Wnts involves the binding of Wnts to receptor complexes at the surface of target cells. These receptor complexes are commonly formed between a member of the Frizzled family of seven-pass transmembrane proteins and a co-receptor, which is usually a single-pass transmembrane protein. Among these co-receptors are several with structural homology to receptor tyrosine kinases, including Ror, PTK7, Ryk and MUSK. In vertebrates, Ror-2 and PTK7 are important regulators of planar cell polarity (PCP). By contrast, PCP phenotypes were not reported for mutations in off-track (otk) and off-track2 (otk2), encoding the Drosophila orthologs of PTK7. Here we show that Drosophila Ror is expressed in the nervous system and localizes to the plasma membrane of perikarya and neurites. A null allele of Ror is homozygous viable and fertile, does not display PCP phenotypes and interacts genetically with mutations in otk and otk2 We show that Ror binds specifically to Wingless (Wg), Wnt4 and Wnt5 and also to Frizzled2 (Fz2) and Otk. Our findings establish Drosophila Ror as a Wnt co-receptor expressed in the nervous system.

KEYWORDS:

Nervous system development; Planar cell polarity; Ror; Wnt signaling

Conflict of interest statement

Competing interestsThe authors declare no competing or financial interests.

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