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eNeuro. 2020 Jan 10;7(1). pii: ENEURO.0213-19.2019. doi: 10.1523/ENEURO.0213-19.2019. Print 2020 Jan/Feb.

Odor-Induced Multi-Level Inhibitory Maps in Drosophila.

Author information

1
Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Jena 07745, Germany.
2
Department of Molecular Neurobiology of Behavior, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, University of Göttingen, Göttingen 37077, Germany.
3
Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Jena 07745, Germany ssachse@ice.mpg.de.

Abstract

Optical imaging of intracellular Ca2+ influx as a correlate of neuronal excitation represents a standard technique for visualizing spatiotemporal activity of neuronal networks. However, the information-processing properties of single neurons and neuronal circuits likewise involve inhibition of neuronal membrane potential. Here, we report spatially resolved optical imaging of odor-evoked inhibitory patterns in the olfactory circuitry of Drosophila using a genetically encoded fluorescent Cl- sensor. In combination with the excitatory component reflected by intracellular Ca2+ dynamics, we present a comprehensive functional map of both odor-evoked neuronal activation and inhibition at different levels of olfactory processing. We demonstrate that odor-evoked inhibition carried by Cl- influx is present both in sensory neurons and second-order projection neurons (PNs), and is characterized by stereotypic, odor-specific patterns. Cl--mediated inhibition features distinct dynamics in different neuronal populations. Our data support a dual role of inhibitory neurons in the olfactory system: global gain control across the neuronal circuitry and glomerulus-specific inhibition to enhance neuronal information processing.

KEYWORDS:

Drosophila; antennal lobe; chloride imaging; inhibition; olfactory coding; sensory processing

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