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Improved production of poly(lactic acid)-like polyester based on metabolite analysis to address the rate-limiting step.

Matsumoto K, Tobitani K, Aoki S, Song Y, Ooi T, Taguchi S.

AMB Express. 2014 Dec;4(1):83. doi: 10.1186/s13568-014-0083-2. Epub 2014 Nov 18.


Directed evolution and structural analysis of NADPH-dependent Acetoacetyl Coenzyme A (Acetoacetyl-CoA) reductase from Ralstonia eutropha reveals two mutations responsible for enhanced kinetics.

Matsumoto K, Tanaka Y, Watanabe T, Motohashi R, Ikeda K, Tobitani K, Yao M, Tanaka I, Taguchi S.

Appl Environ Microbiol. 2013 Oct;79(19):6134-9. doi: 10.1128/AEM.01768-13. Epub 2013 Aug 2.

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