Format

Send to

Choose Destination
Biol Open. 2016 Oct 15;5(10):1473-1484. doi: 10.1242/bio.015826.

Zebrafish P54 RNA helicases are cytoplasmic granule residents that are required for development and stress resilience.

Author information

1
EvoDevo Laboratory, Unidad de Sistemas Arrecifales, Instituto de Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, Puerto Morelos, Quintana Roo, México, 77580.
2
Departamento de Biología Celular y del Desarrollo, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, D.F. México, México, 04510.
3
Laboratory of Genetics, University of Wisconsin-Madison, Wisconsin 53706, USA.
4
EvoDevo Laboratory, Unidad de Sistemas Arrecifales, Instituto de Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, Puerto Morelos, Quintana Roo, México, 77580 ernesto@cmarl.unam.mx.

Abstract

Stress granules are cytoplasmic foci that directly respond to the protein synthesis status of the cell. Various environmental insults, such as oxidative stress or extreme heat, block protein synthesis; consequently, mRNA will stall in translation, and stress granules will immediately form and become enriched with mRNAs. P54 DEAD box RNA helicases are components of RNA granules such as P-bodies and stress granules. We studied the expression, in cytoplasmic foci, of both zebrafish P54 RNA helicases (P54a and P54b) during development and found that they are expressed in cytoplasmic granules under both normal conditions and stress conditions. In zebrafish embryos exposed to heat shock, some proportion of P54a and P54b helicases move to larger granules that exhibit the properties of genuine stress granules. Knockdown of P54a and/or P54b in zebrafish embryos produces developmental abnormalities restricted to the posterior trunk; further, these embryos do not form stress granules, and their survival upon exposure to heat-shock conditions is compromised. Our observations fit the model that cells lacking stress granules have no resilience or ability to recover once the stress has ended, indicating that stress granules play an essential role in the way organisms adapt to a changing environment.

KEYWORDS:

P-bodies; P54 RNA helicase; Stress granules; Zebrafish

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center