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Items: 1 to 50 of 111

1.

Benchmarking Smartphone Fluorescence-Based Microscopy with DNA Origami Nanobeads: Reducing the Gap toward Single-Molecule Sensitivity.

Vietz C, Schütte ML, Wei Q, Richter L, Lalkens B, Ozcan A, Tinnefeld P, Acuna GP.

ACS Omega. 2019 Jan 31;4(1):637-642. doi: 10.1021/acsomega.8b03136. Epub 2019 Jan 9.

PMID:
30775643
2.

Interchromophoric Interactions Determine the Maximum Brightness Density in DNA Origami Structures.

Schröder T, Scheible MB, Steiner F, Vogelsang J, Tinnefeld P.

Nano Lett. 2019 Feb 13;19(2):1275-1281. doi: 10.1021/acs.nanolett.8b04845. Epub 2019 Jan 25.

PMID:
30681342
3.

Publisher Correction: Precision and accuracy of single-molecule FRET measurements-a multi-laboratory benchmark study.

Hellenkamp B, Schmid S, Doroshenko O, Opanasyuk O, Kühnemuth R, Adariani SR, Ambrose B, Aznauryan M, Barth A, Birkedal V, Bowen ME, Chen H, Cordes T, Eilert T, Fijen C, Gebhardt C, Götz M, Gouridis G, Gratton E, Ha T, Hao P, Hanke CA, Hartmann A, Hendrix J, Hildebrandt LL, Hirschfeld V, Hohlbein J, Hua B, Hübner CG, Kallis E, Kapanidis AN, Kim JY, Krainer G, Lamb DC, Lee NK, Lemke EA, Levesque B, Levitus M, McCann JJ, Naredi-Rainer N, Nettels D, Ngo T, Qiu R, Robb NC, Röcker C, Sanabria H, Schlierf M, Schröder T, Schuler B, Seidel H, Streit L, Thurn J, Tinnefeld P, Tyagi S, Vandenberk N, Vera AM, Weninger KR, Wünsch B, Yanez-Orozco IS, Michaelis J, Seidel CAM, Craggs TD, Hugel T.

Nat Methods. 2018 Nov;15(11):984. doi: 10.1038/s41592-018-0193-x.

PMID:
30327572
4.

Precision and accuracy of single-molecule FRET measurements-a multi-laboratory benchmark study.

Hellenkamp B, Schmid S, Doroshenko O, Opanasyuk O, Kühnemuth R, Rezaei Adariani S, Ambrose B, Aznauryan M, Barth A, Birkedal V, Bowen ME, Chen H, Cordes T, Eilert T, Fijen C, Gebhardt C, Götz M, Gouridis G, Gratton E, Ha T, Hao P, Hanke CA, Hartmann A, Hendrix J, Hildebrandt LL, Hirschfeld V, Hohlbein J, Hua B, Hübner CG, Kallis E, Kapanidis AN, Kim JY, Krainer G, Lamb DC, Lee NK, Lemke EA, Levesque B, Levitus M, McCann JJ, Naredi-Rainer N, Nettels D, Ngo T, Qiu R, Robb NC, Röcker C, Sanabria H, Schlierf M, Schröder T, Schuler B, Seidel H, Streit L, Thurn J, Tinnefeld P, Tyagi S, Vandenberk N, Vera AM, Weninger KR, Wünsch B, Yanez-Orozco IS, Michaelis J, Seidel CAM, Craggs TD, Hugel T.

Nat Methods. 2018 Sep;15(9):669-676. doi: 10.1038/s41592-018-0085-0. Epub 2018 Aug 31. Erratum in: Nat Methods. 2018 Nov;15(11):984.

5.

Towards structural biology with super-resolution microscopy.

Molle J, Jakob L, Bohlen J, Raab M, Tinnefeld P, Grohmann D.

Nanoscale. 2018 Sep 13;10(35):16416-16424. doi: 10.1039/c8nr03361g.

PMID:
30141803
6.

Axial Colocalization of Single Molecules with Nanometer Accuracy Using Metal-Induced Energy Transfer.

Isbaner S, Karedla N, Kaminska I, Ruhlandt D, Raab M, Bohlen J, Chizhik A, Gregor I, Tinnefeld P, Enderlein J, Tsukanov R.

Nano Lett. 2018 Apr 11;18(4):2616-2622. doi: 10.1021/acs.nanolett.8b00425. Epub 2018 Mar 27.

PMID:
29562123
7.

Optical Voltage Sensing Using DNA Origami.

Hemmig EA, Fitzgerald C, Maffeo C, Hecker L, Ochmann SE, Aksimentiev A, Tinnefeld P, Keyser UF.

Nano Lett. 2018 Mar 14;18(3):1962-1971. doi: 10.1021/acs.nanolett.7b05354. Epub 2018 Feb 21.

8.

Site-Specific Labelling of Native Mammalian Proteins for Single-Molecule FRET Measurements.

Gust A, Jakob L, Zeitler DM, Bruckmann A, Kramm K, Willkomm S, Tinnefeld P, Meister G, Grohmann D.

Chembiochem. 2018 Apr 16;19(8):780-783. doi: 10.1002/cbic.201700696. Epub 2018 Mar 6.

PMID:
29394002
9.

Using DNA origami nanorulers as traceable distance measurement standards and nanoscopic benchmark structures.

Raab M, Jusuk I, Molle J, Buhr E, Bodermann B, Bergmann D, Bosse H, Tinnefeld P.

Sci Rep. 2018 Jan 29;8(1):1780. doi: 10.1038/s41598-018-19905-x.

10.

Strong Plasmonic Enhancement of a Single Peridinin-Chlorophyll a-Protein Complex on DNA Origami-Based Optical Antennas.

Kaminska I, Bohlen J, Mackowski S, Tinnefeld P, Acuna GP.

ACS Nano. 2018 Feb 27;12(2):1650-1655. doi: 10.1021/acsnano.7b08233. Epub 2018 Jan 29.

PMID:
29353479
11.

Optical Nanoantenna for Single Molecule-Based Detection of Zika Virus Nucleic Acids without Molecular Multiplication.

Ochmann SE, Vietz C, Trofymchuk K, Acuna GP, Lalkens B, Tinnefeld P.

Anal Chem. 2017 Dec 5;89(23):13000-13007. doi: 10.1021/acs.analchem.7b04082. Epub 2017 Nov 22.

PMID:
29144729
12.

Synergistic Combination of Unquenching and Plasmonic Fluorescence Enhancement in Fluorogenic Nucleic Acid Hybridization Probes.

Vietz C, Lalkens B, Acuna GP, Tinnefeld P.

Nano Lett. 2017 Oct 11;17(10):6496-6500. doi: 10.1021/acs.nanolett.7b03844. Epub 2017 Sep 29.

PMID:
28956440
13.

A DNA Walker as a Fluorescence Signal Amplifier.

Wang D, Vietz C, Schröder T, Acuna G, Lalkens B, Tinnefeld P.

Nano Lett. 2017 Sep 13;17(9):5368-5374. doi: 10.1021/acs.nanolett.7b01829. Epub 2017 Aug 7.

PMID:
28750513
14.

Generation of Recombinant Antibodies against the beta-(1,6)-Branched beta-(1,3)-D-Glucan Schizophyllan from Immunized Mice via Phage Display.

Josewski J, Buchmeier S, Frenzel A, Tinnefeld P, Dübel S, Rau U.

Biotechnol Res Int. 2017;2017:8791359. doi: 10.1155/2017/8791359. Epub 2017 May 23.

15.

Plasmonics Enhanced Smartphone Fluorescence Microscopy.

Wei Q, Acuna G, Kim S, Vietz C, Tseng D, Chae J, Shir D, Luo W, Tinnefeld P, Ozcan A.

Sci Rep. 2017 May 18;7(1):2124. doi: 10.1038/s41598-017-02395-8.

16.

Broadband Fluorescence Enhancement with Self-Assembled Silver Nanoparticle Optical Antennas.

Vietz C, Kaminska I, Sanz Paz M, Tinnefeld P, Acuna GP.

ACS Nano. 2017 May 23;11(5):4969-4975. doi: 10.1021/acsnano.7b01621. Epub 2017 Apr 28.

PMID:
28445644
17.

Guide-independent DNA cleavage by archaeal Argonaute from Methanocaldococcus jannaschii.

Zander A, Willkomm S, Ofer S, van Wolferen M, Egert L, Buchmeier S, Stöckl S, Tinnefeld P, Schneider S, Klingl A, Albers SV, Werner F, Grohmann D.

Nat Microbiol. 2017 Mar 20;2:17034. doi: 10.1038/nmicrobiol.2017.34.

PMID:
28319081
18.

Shifting molecular localization by plasmonic coupling in a single-molecule mirage.

Raab M, Vietz C, Stefani FD, Acuna GP, Tinnefeld P.

Nat Commun. 2017 Jan 11;8:13966. doi: 10.1038/ncomms13966.

19.

Repression of RNA polymerase by the archaeo-viral regulator ORF145/RIP.

Sheppard C, Blombach F, Belsom A, Schulz S, Daviter T, Smollett K, Mahieu E, Erdmann S, Tinnefeld P, Garrett R, Grohmann D, Rappsilber J, Werner F.

Nat Commun. 2016 Nov 24;7:13595. doi: 10.1038/ncomms13595.

20.

Molecular force spectroscopy with a DNA origami-based nanoscopic force clamp.

Nickels PC, Wünsch B, Holzmeister P, Bae W, Kneer LM, Grohmann D, Tinnefeld P, Liedl T.

Science. 2016 Oct 21;354(6310):305-307.

PMID:
27846560
21.

A Two-Component Regulatory System Impacts Extracellular Membrane-Derived Vesicle Production in Group A Streptococcus.

Resch U, Tsatsaronis JA, Le Rhun A, Stübiger G, Rohde M, Kasvandik S, Holzmeister S, Tinnefeld P, Wai SN, Charpentier E.

MBio. 2016 Nov 1;7(6). pii: e00207-16. doi: 10.1128/mBio.00207-16.

22.

Fluorescent Nanodiamond-Gold Hybrid Particles for Multimodal Optical and Electron Microscopy Cellular Imaging.

Liu W, Naydenov B, Chakrabortty S, Wuensch B, Hübner K, Ritz S, Cölfen H, Barth H, Koynov K, Qi H, Leiter R, Reuter R, Wrachtrup J, Boldt F, Scheuer J, Kaiser U, Sison M, Lasser T, Tinnefeld P, Jelezko F, Walther P, Wu Y, Weil T.

Nano Lett. 2016 Oct 12;16(10):6236-6244. Epub 2016 Sep 21.

PMID:
27629492
23.

DNA Origami Seesaws as Comparative Binding Assay.

Nickels PC, Høiberg HC, Simmel SS, Holzmeister P, Tinnefeld P, Liedl T.

Chembiochem. 2016 Jun 16;17(12):1093-6. doi: 10.1002/cbic.201600059. Epub 2016 May 6.

24.

TFE and Spt4/5 open and close the RNA polymerase clamp during the transcription cycle.

Schulz S, Gietl A, Smollett K, Tinnefeld P, Werner F, Grohmann D.

Proc Natl Acad Sci U S A. 2016 Mar 29;113(13):E1816-25. doi: 10.1073/pnas.1515817113. Epub 2016 Mar 15. Erratum in: Proc Natl Acad Sci U S A. 2016 May 17;113(20):E2871.

25.

Programming Light-Harvesting Efficiency Using DNA Origami.

Hemmig EA, Creatore C, Wünsch B, Hecker L, Mair P, Parker MA, Emmott S, Tinnefeld P, Keyser UF, Chin AW.

Nano Lett. 2016 Apr 13;16(4):2369-74. doi: 10.1021/acs.nanolett.5b05139. Epub 2016 Mar 1.

26.

Superresolution microscopy with transient binding.

Molle J, Raab M, Holzmeister S, Schmitt-Monreal D, Grohmann D, He Z, Tinnefeld P.

Curr Opin Biotechnol. 2016 Jun;39:8-16. doi: 10.1016/j.copbio.2015.12.009. Epub 2016 Jan 12. Review.

PMID:
26773299
27.

Multifunctional Dumbbell-Shaped DNA-Templated Selective Formation of Fluorescent Silver Nanoclusters or Copper Nanoparticles for Sensitive Detection of Biomolecules.

Chen J, Ji X, Tinnefeld P, He Z.

ACS Appl Mater Interfaces. 2016 Jan 27;8(3):1786-94. doi: 10.1021/acsami.5b09678. Epub 2016 Jan 12.

PMID:
26719979
28.

DNA Origami Nanoantennas with over 5000-fold Fluorescence Enhancement and Single-Molecule Detection at 25 μM.

Puchkova A, Vietz C, Pibiri E, Wünsch B, Sanz Paz M, Acuna GP, Tinnefeld P.

Nano Lett. 2015 Dec 9;15(12):8354-9. doi: 10.1021/acs.nanolett.5b04045. Epub 2015 Nov 5.

PMID:
26523768
29.

Super-Resolution Imaging Conditions for enhanced Yellow Fluorescent Protein (eYFP) Demonstrated on DNA Origami Nanorulers.

Jusuk I, Vietz C, Raab M, Dammeyer T, Tinnefeld P.

Sci Rep. 2015 Sep 16;5:14075. doi: 10.1038/srep14075.

30.

Mapping molecules in scanning far-field fluorescence nanoscopy.

Ta H, Keller J, Haltmeier M, Saka SK, Schmied J, Opazo F, Tinnefeld P, Munk A, Hell SW.

Nat Commun. 2015 Aug 13;6:7977. doi: 10.1038/ncomms8977.

31.

Simple and aberration-free 4color-STED--multiplexing by transient binding.

Beater S, Holzmeister P, Lalkens B, Tinnefeld P.

Opt Express. 2015 Apr 6;23(7):8630-8. doi: 10.1364/OE.23.008630.

PMID:
25968701
32.

Absolute Arrangement of Subunits in Cytoskeletal Septin Filaments in Cells Measured by Fluorescence Microscopy.

Kaplan C, Jing B, Winterflood CM, Bridges AA, Occhipinti P, Schmied J, Grinhagens S, Gronemeyer T, Tinnefeld P, Gladfelter AS, Ries J, Ewers H.

Nano Lett. 2015 Jun 10;15(6):3859-64. doi: 10.1021/acs.nanolett.5b00693. Epub 2015 May 8.

PMID:
25939363
33.

Enzymatic characterization of recombinant nitrate reductase expressed and purified from Neurospora crassa.

Ringel P, Probst C, Dammeyer T, Buchmeier S, Jänsch L, Wissing J, Tinnefeld P, Mendel RR, Jockusch BM, Kruse T.

Fungal Genet Biol. 2015 Jul;80:10-8. doi: 10.1016/j.fgb.2015.04.016. Epub 2015 Apr 23.

PMID:
25914160
34.

Enzymatic polymerization of poly(thymine) for the synthesis of copper nanoparticles with tunable size and their application in enzyme sensing.

Ye T, Li C, Su C, Ji X, Zheng J, Tinnefeld P, He Z.

Chem Commun (Camb). 2015 May 21;51(41):8644-7. doi: 10.1039/c5cc01517k.

PMID:
25899921
35.

Quantum yield and excitation rate of single molecules close to metallic nanostructures.

Holzmeister P, Pibiri E, Schmied JJ, Sen T, Acuna GP, Tinnefeld P.

Nat Commun. 2014 Nov 5;5:5356. doi: 10.1038/ncomms6356.

36.

A starting point for fluorescence-based single-molecule measurements in biomolecular research.

Gust A, Zander A, Gietl A, Holzmeister P, Schulz S, Lalkens B, Tinnefeld P, Grohmann D.

Molecules. 2014 Sep 30;19(10):15824-65. doi: 10.3390/molecules191015824. Review.

37.

Toward quantitative fluorescence microscopy with DNA origami nanorulers.

Beater S, Raab M, Tinnefeld P.

Methods Cell Biol. 2014;123:449-66. doi: 10.1016/B978-0-12-420138-5.00024-0.

PMID:
24974042
38.

Enhancing single-molecule fluorescence with nanophotonics.

Acuna G, Grohmann D, Tinnefeld P.

FEBS Lett. 2014 Oct 1;588(19):3547-52. doi: 10.1016/j.febslet.2014.06.016. Epub 2014 Jun 10. Review.

39.

Fluorescence microscopy with 6 nm resolution on DNA origami.

Raab M, Schmied JJ, Jusuk I, Forthmann C, Tinnefeld P.

Chemphyschem. 2014 Aug 25;15(12):2431-5. doi: 10.1002/cphc.201402179. Epub 2014 Jun 4.

PMID:
24895173
40.

DNA origami-based standards for quantitative fluorescence microscopy.

Schmied JJ, Raab M, Forthmann C, Pibiri E, Wünsch B, Dammeyer T, Tinnefeld P.

Nat Protoc. 2014;9(6):1367-91. doi: 10.1038/nprot.2014.079. Epub 2014 May 15.

PMID:
24833175
41.

Single-molecule positioning in zeromode waveguides by DNA origami nanoadapters.

Pibiri E, Holzmeister P, Lalkens B, Acuna GP, Tinnefeld P.

Nano Lett. 2014 Jun 11;14(6):3499-503. doi: 10.1021/nl501064b. Epub 2014 May 8.

PMID:
24773477
42.

Eukaryotic and archaeal TBP and TFB/TF(II)B follow different promoter DNA bending pathways.

Gietl A, Holzmeister P, Blombach F, Schulz S, von Voithenberg LV, Lamb DC, Werner F, Tinnefeld P, Grohmann D.

Nucleic Acids Res. 2014 Jun;42(10):6219-31. doi: 10.1093/nar/gku273. Epub 2014 Apr 17.

43.

Geminate recombination as a photoprotection mechanism for fluorescent dyes.

Holzmeister P, Gietl A, Tinnefeld P.

Angew Chem Int Ed Engl. 2014 May 26;53(22):5685-8. doi: 10.1002/anie.201310300. Epub 2014 Apr 8.

PMID:
24715383
44.

Controlled reduction of photobleaching in DNA origami-gold nanoparticle hybrids.

Pellegrotti JV, Acuna GP, Puchkova A, Holzmeister P, Gietl A, Lalkens B, Stefani FD, Tinnefeld P.

Nano Lett. 2014 May 14;14(5):2831-6. doi: 10.1021/nl500841n. Epub 2014 Apr 4.

PMID:
24690008
45.

Choosing dyes for cw-STED nanoscopy using self-assembled nanorulers.

Beater S, Holzmeister P, Pibiri E, Lalkens B, Tinnefeld P.

Phys Chem Chem Phys. 2014 Apr 21;16(15):6990-6. doi: 10.1039/c4cp00127c. Epub 2014 Mar 6.

46.

Single-molecule FRET supports the two-state model of Argonaute action.

Zander A, Holzmeister P, Klose D, Tinnefeld P, Grohmann D.

RNA Biol. 2014;11(1):45-56. doi: 10.4161/rna.27446. Epub 2013 Dec 20.

47.

Single-molecule photophysics of dark quenchers as non-fluorescent FRET acceptors.

Holzmeister P, Wünsch B, Gietl A, Tinnefeld P.

Photochem Photobiol Sci. 2014 Jun;13(6):853-8. doi: 10.1039/c3pp50274k.

PMID:
24100609
48.

Breaking the concentration limit of optical single-molecule detection.

Holzmeister P, Acuna GP, Grohmann D, Tinnefeld P.

Chem Soc Rev. 2014 Feb 21;43(4):1014-28. doi: 10.1039/c3cs60207a. Epub 2013 Sep 10. Review.

PMID:
24019005
49.

Counting fluorescent dye molecules on DNA origami by means of photon statistics.

Kurz A, Schmied JJ, Grußmayer KS, Holzmeister P, Tinnefeld P, Herten DP.

Small. 2013 Dec 9;9(23):4061-8. doi: 10.1002/smll.201300619. Epub 2013 Jun 24.

PMID:
23794455
50.

Single-molecule detection: Breaking the concentration barrier.

Tinnefeld P.

Nat Nanotechnol. 2013 Jul;8(7):480-2. doi: 10.1038/nnano.2013.122. Epub 2013 Jun 16. No abstract available.

PMID:
23770809

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