Format

Send to

Choose Destination
Elife. 2019 May 21;8. pii: e43079. doi: 10.7554/eLife.43079.

Neurogenetic dissection of the Drosophila lateral horn reveals major outputs, diverse behavioural functions, and interactions with the mushroom body.

Author information

1
Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, United States.
2
Division of Neurobiology, MRC Laboratory of Molecular Biology, Cambridge, United Kingdom.
3
Department of Zoology, University of Cambridge, Cambridge, United Kingdom.
#
Contributed equally

Abstract

Animals exhibit innate behaviours to a variety of sensory stimuli including olfactory cues. In Drosophila, one higher olfactory centre, the lateral horn (LH), is implicated in innate behaviour. However, our structural and functional understanding of the LH is scant, in large part due to a lack of sparse neurogenetic tools for this region. We generate a collection of split-GAL4 driver lines providing genetic access to 82 LH cell types. We use these to create an anatomical and neurotransmitter map of the LH and link this to EM connectomics data. We find ~30% of LH projections converge with outputs from the mushroom body, site of olfactory learning and memory. Using optogenetic activation, we identify LH cell types that drive changes in valence behavior or specific locomotor programs. In summary, we have generated a resource for manipulating and mapping LH neurons, providing new insights into the circuit basis of innate and learned olfactory behavior.

KEYWORDS:

D. melanogaster; behaviour; innate; lateral Horn; learning; memory; neurogenetics; neuroscience

Supplemental Content

Full text links

Icon for eLife Sciences Publications, Ltd Icon for PubMed Central
Loading ...
Support Center