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Jundishapur J Microbiol. 2014 May;7(5):e10020. doi: 10.5812/jjm.10020. Epub 2014 May 1.

Biofilm Formation by Staphylococcus epidermidis on Foldable and Rigid Intraocular Lenses.

Author information

1
Biotechnology Research Centre, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IR Iran.
2
School of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran.
3
Department of Food and Drug Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IR Iran.

Abstract

BACKGROUND:

Biofilm formation of Staphylococcus epidermidis is a major etiological factor of inducing device-related infections.

OBJECTIVES:

The ability of biofilm formation by the S. epidermidis was assessed in vitro on two brands of foldable (hydrophilic) and two brands of rigid (hydrophobic) intraocular lens materials in order to investigate the role of lens material in postoperative endophthalmitis.

MATERIALS AND METHODS:

To ensure reproducibility of biofilm formation on intraocular lenses, two strains of S. epidermidis and three quantification methods were performed. The S. epidermidis strains, DSMZ3270 (biofilm-producer) and ATCC12228 (non-biofilm-producer) were applied. Organisms were cultivated on disks of different brands of foldable hydrophilic Intra Ocular Lens (IOL) made of acrylic (Didar, Iran; (A) and Omni, India; (B)), and rigid hydrophobic IOL made of polymethyl methacrylate (PMMA; Didar, Iran; (C) and Hexavision, France; (D)). Biofilms were stained with crystal violet (CV) dye, which is an index of biofilm formation. The bacterial population was counted after biofilm homogenization. Scanning electron microscopy (SEM) was performed to examine the extent of biofilm formation.

RESULTS:

Adherence of DSMZ3270 strain on both types of foldable and rigid IOLs, was significantly more than ATCC12228 (P < 0.001-0.05 and, P < 0.01-0.05, respectively). The bacterial populations between the lenses were significantly different (P < 0.05). Subsequent studies demonstrated significant differences between brands of foldable and PMMA IOLs. According to statistical analyses the incubation time influenced the biofilm formation on both types of IOLs which meant that by increasing incubation time, the biofilm formation increased. According to the SEM pictures, biofilm seems to be lysed at 72 hours.

CONCLUSIONS:

These data demonstrated that the attachment of bacteria to hydrophilic acrylic IOLs was more than hydrophobic PMMA ones independent of the brand. According to these results the bacterial strain might have more hydrophilic properties. Augmenting the biomass of biofilm by passing of time demonstrated the key role of time in biofilm formation on the IOL surfaces. The differences between IOL brands in the biofilm formation indicated the influence of design parameters for IOLs.

KEYWORDS:

Biofilms; Endophthalmitis; Lenses, Intraocular; Staphylococcus epidermidis

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