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Proc Natl Acad Sci U S A. 2002 Aug 20;99(17):11049-54. Epub 2002 Aug 12.

Global analysis of the Deinococcus radiodurans proteome by using accurate mass tags.

Author information

1
Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, P.O. Box 999, MSIN: K8-98, Richland, WA 99352, USA.

Abstract

Understanding biological systems and the roles of their constituents is facilitated by the ability to make quantitative, sensitive, and comprehensive measurements of how their proteome changes, e.g., in response to environmental perturbations. To this end, we have developed a high-throughput methodology to characterize an organism's dynamic proteome based on the combination of global enzymatic digestion, high-resolution liquid chromatographic separations, and analysis by Fourier transform ion cyclotron resonance mass spectrometry. The peptides produced serve as accurate mass tags for the proteins and have been used to identify with high confidence >61% of the predicted proteome for the ionizing radiation-resistant bacterium Deinococcus radiodurans. This fraction represents the broadest proteome coverage for any organism to date and includes 715 proteins previously annotated as either hypothetical or conserved hypothetical.

PMID:
12177431
PMCID:
PMC129300
DOI:
10.1073/pnas.172170199
[Indexed for MEDLINE]
Free PMC Article

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