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Items: 1 to 50 of 62


Chromatin-Associated RNA Sequencing (ChAR-seq).

Jukam D, Limouse C, Smith OK, Risca VI, Bell JC, Straight AF.

Curr Protoc Mol Biol. 2019 Apr;126(1):e87. doi: 10.1002/cpmb.87. Epub 2019 Feb 20.


CDK phosphorylation of Xenopus laevis M18BP1 promotes its metaphase centromere localization.

French BT, Straight AF.

EMBO J. 2019 Feb 15;38(4). pii: e100093. doi: 10.15252/embj.2018100093. Epub 2019 Jan 2.


Chromatin-associated RNA sequencing (ChAR-seq) maps genome-wide RNA-to-DNA contacts.

Bell JC, Jukam D, Teran NA, Risca VI, Smith OK, Johnson WL, Skotheim JM, Greenleaf WJ, Straight AF.

Elife. 2018 Apr 12;7. pii: e27024. doi: 10.7554/eLife.27024.


Measurement of Mesoscale Conformational Dynamics of Freely Diffusing Molecules with Tracking FCS.

Limouse C, Bell JC, Fuller CJ, Straight AF, Mabuchi H.

Biophys J. 2018 Apr 10;114(7):1539-1550. doi: 10.1016/j.bpj.2018.01.044.


Centromere and Kinetochore Assembly in Xenopus laevis Egg Extract.

Flores Servin JC, Straight AF.

Cold Spring Harb Protoc. 2018 Sep 4;2018(9). doi: 10.1101/pdb.prot102509.


Constitutive centromere-associated network contacts confer differential stability on CENP-A nucleosomes in vitro and in the cell.

Cao S, Zhou K, Zhang Z, Luger K, Straight AF.

Mol Biol Cell. 2018 Mar 15;29(6):751-762. doi: 10.1091/mbc.E17-10-0596. Epub 2018 Jan 17.


The Power of Xenopus Egg Extract for Reconstitution of Centromere and Kinetochore Function.

French BT, Straight AF.

Prog Mol Subcell Biol. 2017;56:59-84. doi: 10.1007/978-3-319-58592-5_3. Review.


RNA-dependent stabilization of SUV39H1 at constitutive heterochromatin.

Johnson WL, Yewdell WT, Bell JC, McNulty SM, Duda Z, O'Neill RJ, Sullivan BA, Straight AF.

Elife. 2017 Aug 1;6. pii: e25299. doi: 10.7554/eLife.25299.


Xenopus laevis M18BP1 Directly Binds Existing CENP-A Nucleosomes to Promote Centromeric Chromatin Assembly.

French BT, Westhorpe FG, Limouse C, Straight AF.

Dev Cell. 2017 Jul 24;42(2):190-199.e10. doi: 10.1016/j.devcel.2017.06.021.


Editorial overview: The cell nucleus: New discoveries on nuclear structure, dynamics and function.

Foisner R, Straight AF.

Curr Opin Cell Biol. 2017 Jun;46:iv-vi. doi: 10.1016/ No abstract available.


RNA-mediated regulation of heterochromatin.

Johnson WL, Straight AF.

Curr Opin Cell Biol. 2017 Jun;46:102-109. doi: 10.1016/ Epub 2017 Jun 11. Review.


Form and function of topologically associating genomic domains in budding yeast.

Eser U, Chandler-Brown D, Ay F, Straight AF, Duan Z, Noble WS, Skotheim JM.

Proc Natl Acad Sci U S A. 2017 Apr 11;114(15):E3061-E3070. doi: 10.1073/pnas.1612256114. Epub 2017 Mar 27. Erratum in: Proc Natl Acad Sci U S A. 2017 Oct 10;114(41):E8801.


Variable chromatin structure revealed by in situ spatially correlated DNA cleavage mapping.

Risca VI, Denny SK, Straight AF, Greenleaf WJ.

Nature. 2017 Jan 12;541(7636):237-241. doi: 10.1038/nature20781. Epub 2016 Dec 26.


Chromosome Segregation: Reconstituting the Kinetochore.

Westhorpe FG, Straight AF.

Curr Biol. 2016 Dec 5;26(23):R1242-R1245. doi: 10.1016/j.cub.2016.09.051.


MIS12/MIND Control at the Kinetochore.

Ladurner R, Straight AF.

Cell. 2016 Nov 3;167(4):889-891. doi: 10.1016/j.cell.2016.10.036.


Acetylation of histone H4 lysine 5 and 12 is required for CENP-A deposition into centromeres.

Shang WH, Hori T, Westhorpe FG, Godek KM, Toyoda A, Misu S, Monma N, Ikeo K, Carroll CW, Takami Y, Fujiyama A, Kimura H, Straight AF, Fukagawa T.

Nat Commun. 2016 Nov 4;7:13465. doi: 10.1038/ncomms13465.


In Vitro Kinetochore Assembly.

Miell MD, Straight AF.

Methods Mol Biol. 2016;1413:111-33. doi: 10.1007/978-1-4939-3542-0_8.


Condensing chromosome condensation.

Bell JC, Straight AF.

Nat Cell Biol. 2015 Aug;17(8):964-5. doi: 10.1038/ncb3212.


A cell-free CENP-A assembly system defines the chromatin requirements for centromere maintenance.

Westhorpe FG, Fuller CJ, Straight AF.

J Cell Biol. 2015 Jun 22;209(6):789-801. doi: 10.1083/jcb.201503132. Epub 2015 Jun 15.


Histone titration against the genome sets the DNA-to-cytoplasm threshold for the Xenopus midblastula transition.

Amodeo AA, Jukam D, Straight AF, Skotheim JM.

Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):E1086-95. doi: 10.1073/pnas.1413990112. Epub 2015 Feb 23.


Regulating the timing of CENP-A nucleosome assembly by phosphorylation.

Miell MD, Straight AF.

Dev Cell. 2015 Jan 12;32(1):1-2. doi: 10.1016/j.devcel.2014.12.020.


The centromere: epigenetic control of chromosome segregation during mitosis.

Westhorpe FG, Straight AF.

Cold Spring Harb Perspect Biol. 2014 Nov 20;7(1):a015818. doi: 10.1101/cshperspect.a015818. Review.


Reply to "CENP-A octamers do not confer a reduction in nucleosome height by AFM".

Miell MD, Straight AF, Allshire RC.

Nat Struct Mol Biol. 2014 Jan;21(1):5-8. doi: 10.1038/nsmb.2744. No abstract available.


Swapping CENP-A at the centromere.

French BT, Straight AF.

Nat Cell Biol. 2013 Sep;15(9):1028-30. doi: 10.1038/ncb2833.


Fluorescent protein applications in microscopy.

Johnson WL, Straight AF.

Methods Cell Biol. 2013;114:99-123. doi: 10.1016/B978-0-12-407761-4.00005-1.


A conserved mechanism for centromeric nucleosome recognition by centromere protein CENP-C.

Kato H, Jiang J, Zhou BR, Rozendaal M, Feng H, Ghirlando R, Xiao TS, Straight AF, Bai Y.

Science. 2013 May 31;340(6136):1110-3. doi: 10.1126/science.1235532.


CENP-A confers a reduction in height on octameric nucleosomes.

Miell MD, Fuller CJ, Guse A, Barysz HM, Downes A, Owen-Hughes T, Rappsilber J, Straight AF, Allshire RC.

Nat Struct Mol Biol. 2013 Jun;20(6):763-5. doi: 10.1038/nsmb.2574. Epub 2013 May 5.


Esperanto for histones: CENP-A, not CenH3, is the centromeric histone H3 variant.

Earnshaw WC, Allshire RC, Black BE, Bloom K, Brinkley BR, Brown W, Cheeseman IM, Choo KH, Copenhaver GP, Deluca JG, Desai A, Diekmann S, Erhardt S, Fitzgerald-Hayes M, Foltz D, Fukagawa T, Gassmann R, Gerlich DW, Glover DM, Gorbsky GJ, Harrison SC, Heun P, Hirota T, Jansen LE, Karpen G, Kops GJ, Lampson MA, Lens SM, Losada A, Luger K, Maiato H, Maddox PS, Margolis RL, Masumoto H, McAinsh AD, Mellone BG, Meraldi P, Musacchio A, Oegema K, O'Neill RJ, Salmon ED, Scott KC, Straight AF, Stukenberg PT, Sullivan BA, Sullivan KF, Sunkel CE, Swedlow JR, Walczak CE, Warburton PE, Westermann S, Willard HF, Wordeman L, Yanagida M, Yen TJ, Yoda K, Cleveland DW.

Chromosome Res. 2013 Apr;21(2):101-6. doi: 10.1007/s10577-013-9347-y. Epub 2013 Apr 12.


Functions of the centromere and kinetochore in chromosome segregation.

Westhorpe FG, Straight AF.

Curr Opin Cell Biol. 2013 Jun;25(3):334-40. doi: 10.1016/ Epub 2013 Mar 13. Review.


A cell-free system for functional centromere and kinetochore assembly.

Guse A, Fuller CJ, Straight AF.

Nat Protoc. 2012 Oct;7(10):1847-69. doi: 10.1038/nprot.2012.112. Epub 2012 Sep 20.


Imaging nanometre-scale structure in cells using in situ aberration correction.

Fuller CJ, Straight AF.

J Microsc. 2012 Oct;248(1):90-101. doi: 10.1111/j.1365-2818.2012.03654.x. Epub 2012 Aug 20.


The split personality of CENP-A nucleosomes.

Westhorpe FG, Straight AF.

Cell. 2012 Jul 20;150(2):245-7. doi: 10.1016/j.cell.2012.07.003.


Dynamics of CENP-N kinetochore binding during the cell cycle.

Hellwig D, Emmerth S, Ulbricht T, Döring V, Hoischen C, Martin R, Samora CP, McAinsh AD, Carroll CW, Straight AF, Meraldi P, Diekmann S.

J Cell Sci. 2011 Nov 15;124(Pt 22):3871-83. doi: 10.1242/jcs.088625. Epub 2011 Nov 18.


CENP-C recruits M18BP1 to centromeres to promote CENP-A chromatin assembly.

Moree B, Meyer CB, Fuller CJ, Straight AF.

J Cell Biol. 2011 Sep 19;194(6):855-71. doi: 10.1083/jcb.201106079. Epub 2011 Sep 12.


In vitro centromere and kinetochore assembly on defined chromatin templates.

Guse A, Carroll CW, Moree B, Fuller CJ, Straight AF.

Nature. 2011 Aug 28;477(7364):354-8. doi: 10.1038/nature10379.


Local geometry and elasticity in compact chromatin structure.

Koslover EF, Fuller CJ, Straight AF, Spakowitz AJ.

Biophys J. 2010 Dec 15;99(12):3941-50. doi: 10.1016/j.bpj.2010.10.024.


Dual recognition of CENP-A nucleosomes is required for centromere assembly.

Carroll CW, Milks KJ, Straight AF.

J Cell Biol. 2010 Jun 28;189(7):1143-55. doi: 10.1083/jcb.201001013. Epub 2010 Jun 21.


RB's original CIN?

Sage J, Straight AF.

Genes Dev. 2010 Jul 1;24(13):1329-33. doi: 10.1101/gad.1948010. Epub 2010 Jun 15.


Image analysis benchmarking methods for high-content screen design.

Fuller CJ, Straight AF.

J Microsc. 2010 May;238(2):145-61. doi: 10.1111/j.1365-2818.2009.03337.x.


Dissection of CENP-C-directed centromere and kinetochore assembly.

Milks KJ, Moree B, Straight AF.

Mol Biol Cell. 2009 Oct;20(19):4246-55. doi: 10.1091/mbc.E09-05-0378. Epub 2009 Jul 29.


Centromere assembly requires the direct recognition of CENP-A nucleosomes by CENP-N.

Carroll CW, Silva MC, Godek KM, Jansen LE, Straight AF.

Nat Cell Biol. 2009 Jul;11(7):896-902. doi: 10.1038/ncb1899. Epub 2009 Jun 21.


Genome-wide analysis reveals a cell cycle-dependent mechanism controlling centromere propagation.

Erhardt S, Mellone BG, Betts CM, Zhang W, Karpen GH, Straight AF.

J Cell Biol. 2008 Dec 1;183(5):805-18. doi: 10.1083/jcb.200806038.


Fluorescent protein applications in microscopy.

Straight AF.

Methods Cell Biol. 2007;81:93-113. Review. No abstract available.


Polo-like kinase controls vertebrate spindle elongation and cytokinesis.

Brennan IM, Peters U, Kapoor TM, Straight AF.

PLoS One. 2007 May 2;2(5):e409.


Centromeric chromatin gets loaded.

Carroll CW, Straight AF.

J Cell Biol. 2007 Mar 12;176(6):735-6. Epub 2007 Mar 5.


Centromere formation: from epigenetics to self-assembly.

Carroll CW, Straight AF.

Trends Cell Biol. 2006 Feb;16(2):70-8. Epub 2006 Jan 18. Review.


Blebbistatin, a myosin II inhibitor, is photoinactivated by blue light.

Sakamoto T, Limouze J, Combs CA, Straight AF, Sellers JR.

Biochemistry. 2005 Jan 18;44(2):584-8.


Specificity of blebbistatin, an inhibitor of myosin II.

Limouze J, Straight AF, Mitchison T, Sellers JR.

J Muscle Res Cell Motil. 2004;25(4-5):337-41.


Kinetic mechanism of blebbistatin inhibition of nonmuscle myosin IIb.

Ramamurthy B, Yengo CM, Straight AF, Mitchison TJ, Sweeney HL.

Biochemistry. 2004 Nov 23;43(46):14832-9.


Anillin binds nonmuscle myosin II and regulates the contractile ring.

Straight AF, Field CM, Mitchison TJ.

Mol Biol Cell. 2005 Jan;16(1):193-201. Epub 2004 Oct 20.

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