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Development and Characterization of Monoclonal Antibodies to Botulinum Neurotoxin Type E.

Bever CS, Scotcher M, Cheng LW, Hnasko RM, Stanker LH.

Toxins (Basel). 2019 Jul 13;11(7). pii: E407. doi: 10.3390/toxins11070407.


Influence of Food Matrices on the Stability and Bioavailability of Abrin.

Tam CC, Henderson TD 2nd, Stanker LH, Cheng LW.

Toxins (Basel). 2018 Dec 1;10(12). pii: E502. doi: 10.3390/toxins10120502.


A New Conjugation Method Used for the Development of an Immunoassay for the Detection of Amanitin, a Deadly Mushroom Toxin.

Bever CS, Barnych B, Hnasko R, Cheng LW, Stanker LH.

Toxins (Basel). 2018 Jun 28;10(7). pii: E265. doi: 10.3390/toxins10070265.


Detection of Abrin Holotoxin Using Novel Monoclonal Antibodies.

He X, Patfield S, Cheng LW, Stanker LH, Rasooly R, McKeon TA, Zhang Y, Brandon DL.

Toxins (Basel). 2017 Nov 28;9(12). pii: E386. doi: 10.3390/toxins9120386.


A Monoclonal-Monoclonal Antibody Based Capture ELISA for Abrin.

Tam CC, Cheng LW, He X, Merrill P, Hodge D, Stanker LH.

Toxins (Basel). 2017 Oct 18;9(10). pii: E328. doi: 10.3390/toxins9100328.


Abrin Toxicity and Bioavailability after Temperature and pH Treatment.

Tam CC, Henderson TD, Stanker LH, He X, Cheng LW.

Toxins (Basel). 2017 Oct 13;9(10). pii: E320. doi: 10.3390/toxins9100320.


Identification and verification of hybridoma-derived monoclonal antibody variable region sequences using recombinant DNA technology and mass spectrometry.

Babrak L, McGarvey JA, Stanker LH, Hnasko R.

Mol Immunol. 2017 Oct;90:287-294. doi: 10.1016/j.molimm.2017.08.014. Epub 2017 Sep 1.


Probiotic Microorganisms Inhibit Epithelial Cell Internalization of Botulinum Neurotoxin Serotype A.

Lam TI, Tam CC, Stanker LH, Cheng LW.

Toxins (Basel). 2016 Dec 16;8(12). pii: E377. doi: 10.3390/toxins8120377.


Rapid Microfluidic Assay for the Detection of Botulinum Neurotoxin in Animal Sera.

Babrak L, Lin A, Stanker LH, McGarvey J, Hnasko R.

Toxins (Basel). 2016 Jan 4;8(1). pii: E13. doi: 10.3390/toxins8010013.


Use of Monoclonal Antibodies in the Sensitive Detection and Neutralization of Botulinum Neurotoxin Serotype B.

Cheng LW, Henderson TD 2nd, Lam TI, Stanker LH.

Toxins (Basel). 2015 Nov 27;7(12):5068-78. doi: 10.3390/toxins7124863.


A Double-Sandwich ELISA for Identification of Monoclonal Antibodies Suitable for Sandwich Immunoassays.

Stanker LH, Hnasko RM.

Methods Mol Biol. 2015;1318:69-78. doi: 10.1007/978-1-4939-2742-5_7.


Hybridoma Technology.

Hnasko RM, Stanker LH.

Methods Mol Biol. 2015;1318:15-28. doi: 10.1007/978-1-4939-2742-5_2.


Bacterial populations on the surfaces of organic and conventionally grown almond drupes.

McGarvey JA, Han R, Connell JH, Stanker LH, Hnasko R.

J Appl Microbiol. 2015 Aug;119(2):529-38. doi: 10.1111/jam.12850. Epub 2015 Jun 16.


Detection of shiga toxins by lateral flow assay.

Ching KH, He X, Stanker LH, Lin AV, McGarvey JA, Hnasko R.

Toxins (Basel). 2015 Apr 3;7(4):1163-73. doi: 10.3390/toxins7041163.


Translocation of botulinum neurotoxin serotype A and associated proteins across the intestinal epithelia.

Lam TI, Stanker LH, Lee K, Jin R, Cheng LW.

Cell Microbiol. 2015 Aug;17(8):1133-43. doi: 10.1111/cmi.12424. Epub 2015 Feb 15.


Centrifugal microfluidic platform for ultrasensitive detection of botulinum toxin.

Koh CY, Schaff UY, Piccini ME, Stanker LH, Cheng LW, Ravichandran E, Singh BR, Sommer GJ, Singh AK.

Anal Chem. 2015 Jan 20;87(2):922-8. doi: 10.1021/ac504054u. Epub 2015 Jan 5.


New high-affinity monoclonal antibodies against Shiga toxin 1 facilitate the detection of hybrid Stx1/Stx2 in vivo.

Skinner C, Patfield S, Stanker LH, Fratamico P, He X.

PLoS One. 2014 Jun 10;9(6):e99854. doi: 10.1371/journal.pone.0099854. eCollection 2014.


Use of benzo analogs to enhance antimycotic activity of kresoxim methyl for control of aflatoxigenic fungal pathogens.

Kim JH, Mahoney N, Chan KL, Campbell BC, Haff RP, Stanker LH.

Front Microbiol. 2014 Mar 7;5:87. doi: 10.3389/fmicb.2014.00087. eCollection 2014.


Bacterial population structure and dynamics during the development of almond drupes.

McGarvey JA, Connell JH, Stanker LH, Hnasko R.

J Appl Microbiol. 2014 Jun;116(6):1543-52. doi: 10.1111/jam.12464. Epub 2014 Mar 4.


Atoxic derivative of botulinum neurotoxin A as a prototype molecular vehicle for targeted delivery to the neuronal cytoplasm.

Vazquez-Cintron EJ, Vakulenko M, Band PA, Stanker LH, Johnson EA, Ichtchenko K.

PLoS One. 2014 Jan 22;9(1):e85517. doi: 10.1371/journal.pone.0085517. eCollection 2014.


A monoclonal antibody based capture ELISA for botulinum neurotoxin serotype B: toxin detection in food.

Stanker LH, Scotcher MC, Cheng L, Ching K, McGarvey J, Hodge D, Hnasko R.

Toxins (Basel). 2013 Nov 18;5(11):2212-26. doi: 10.3390/toxins5112212.


Mouse in vivo neutralization of Escherichia coli Shiga toxin 2 with monoclonal antibodies.

Cheng LW, Henderson TD, Patfield S, Stanker LH, He X.

Toxins (Basel). 2013 Oct 22;5(10):1845-58. doi: 10.3390/toxins5101845.


Purification and characterization of neurotoxin complex from a dual toxin gene containing Clostridium Botulinum Strain PS-5.

Singh AK, Sachdeva A, Degrasse JA, Croley TR, Stanker LH, Hodge D, Sharma SK.

Protein J. 2013 Apr;32(4):288-96. doi: 10.1007/s10930-013-9486-1.


Development and characterization of monoclonal antibodies against Shiga toxin 2 and their application for toxin detection in milk.

He X, McMahon S, Skinner C, Merrill P, Scotcher MC, Stanker LH.

J Immunol Methods. 2013 Mar 29;389(1-2):18-28. doi: 10.1016/j.jim.2012.12.005. Epub 2012 Dec 30.


Detection of botulinum neurotoxin serotypes A and B using a chemiluminescent versus electrochemiluminescent immunoassay in food and serum.

Cheng LW, Stanker LH.

J Agric Food Chem. 2013 Jan 23;61(3):755-60. doi: 10.1021/jf3041963. Epub 2013 Jan 10.


Novel epitopes identified by anti-PrP monoclonal antibodies produced following immunization of Prnp0/0 Balb/cJ mice with purified scrapie prions.

Stanker LH, Scotcher MC, Lin A, McGarvey J, Prusiner SB, Hnasko R.

Hybridoma (Larchmt). 2012 Oct;31(5):314-24. doi: 10.1089/hyb.2012.0022.


Detection of botulinum neurotoxin serotype A, B, and F proteolytic activity in complex matrices with picomolar to femtomolar sensitivity.

Dunning FM, Ruge DR, Piazza TM, Stanker LH, Zeytin FN, Tucker WC.

Appl Environ Microbiol. 2012 Nov;78(21):7687-97. doi: 10.1128/AEM.01664-12. Epub 2012 Aug 24.


Botulinum neurotoxin: where are we with detection technologies?

Singh AK, Stanker LH, Sharma SK.

Crit Rev Microbiol. 2013 Feb;39(1):43-56. doi: 10.3109/1040841X.2012.691457. Epub 2012 Jun 8. Review.


Rapid and selective detection of botulinum neurotoxin serotype-A and -B with a single immunochromatographic test strip.

Ching KH, Lin A, McGarvey JA, Stanker LH, Hnasko R.

J Immunol Methods. 2012 Jun 29;380(1-2):23-9. doi: 10.1016/j.jim.2012.03.008. Epub 2012 Apr 4.


A rapid method to improve protein detection by indirect ELISA.

Hnasko R, Lin A, McGarvey JA, Stanker LH.

Biochem Biophys Res Commun. 2011 Jul 15;410(4):726-31. doi: 10.1016/j.bbrc.2011.06.005. Epub 2011 Jun 7.


Effects of sodium bisulfate on the bacterial population structure of dairy cow waste.

McGarvey JA, Stackhouse KR, Miller WG, Stanker LH, Hnasko R, Mitloehner F.

J Appl Microbiol. 2011 Aug;111(2):319-28. doi: 10.1111/j.1365-2672.2011.05047.x. Epub 2011 Jun 9.


Neuronal targeting, internalization, and biological activity of a recombinant atoxic derivative of botulinum neurotoxin A.

Pellett S, Tepp WH, Stanker LH, Band PA, Johnson EA, Ichtchenko K.

Biochem Biophys Res Commun. 2011 Feb 25;405(4):673-7. doi: 10.1016/j.bbrc.2011.01.093. Epub 2011 Feb 1.


Generation of antisera to purified prions in lipid rafts.

Hnasko R, Serban AV, Carlson G, Prusiner SB, Stanker LH.

Prion. 2010 Apr-Jun;4(2):94-104. Epub 2010 Apr 9. Erratum in: Prion. 2011 Jul-Sep;5(3):235.


Detection of botulinum neurotoxin serotype B at sub mouse LD(50) levels by a sandwich immunoassay and its application to toxin detection in milk.

Scotcher MC, Cheng LW, Stanker LH.

PLoS One. 2010 Jun 10;5(6):e11047. doi: 10.1371/journal.pone.0011047.


Conformation-dependent high-affinity monoclonal antibodies to prion proteins.

Stanker LH, Serban AV, Cleveland E, Hnasko R, Lemus A, Safar J, DeArmond SJ, Prusiner SB.

J Immunol. 2010 Jul 1;185(1):729-37. doi: 10.4049/jimmunol.0902930. Epub 2010 Jun 7.


Characterization of the epitope region of F1-2 and F1-5, two monoclonal antibodies to Botulinum neurotoxin type A.

Scotcher MC, Johnson EA, Stanker LH.

Hybridoma (Larchmt). 2009 Oct;28(5):315-25. doi: 10.1089/hyb.2009.0022.


Antibody protection against botulinum neurotoxin intoxication in mice.

Cheng LW, Stanker LH, Henderson TD 2nd, Lou J, Marks JD.

Infect Immun. 2009 Oct;77(10):4305-13. doi: 10.1128/IAI.00405-09. Epub 2009 Aug 3.


Epitope characterization and variable region sequence of f1-40, a high-affinity monoclonal antibody to botulinum neurotoxin type a (Hall strain).

Scotcher MC, McGarvey JA, Johnson EA, Stanker LH.

PLoS One. 2009;4(3):e4924. doi: 10.1371/journal.pone.0004924. Epub 2009 Mar 17. Erratum in: PLoS One. 2009;4(10). doi: 10.1371/annotation/47f9bc3f-7c79-453c-85e3-588bc3056951. PLoS One. 2009;4(10). doi: 10.1371/annotation/6359a6c2-90f8-4f7e-ad86-5bac5d62a7fe. PLoS One. 2009;4(10). doi: 10.1371/annotation/8d83a0a4-4dd0-4c57-ad94-304130f565e6. PLoS One. 2009;4(11). doi: 10.1371/annotation/6359a6c2-90f8-4f7e-ad86-5bac5d62a7fe.


Detection of botulinum neurotoxin-A activity in food by peptide cleavage assay.

Rasooly R, Stanker LH, Carter JM, Do PM, Cheng LW, He X, Brandon DL.

Int J Food Microbiol. 2008 Aug 15;126(1-2):135-9. doi: 10.1016/j.ijfoodmicro.2008.05.012. Epub 2008 May 21.


Effects of purification on the bioavailability of botulinum neurotoxin type A.

Cheng LW, Onisko B, Johnson EA, Reader JR, Griffey SM, Larson AE, Tepp WH, Stanker LH, Brandon DL, Carter JM.

Toxicology. 2008 Jul 30;249(2-3):123-9. doi: 10.1016/j.tox.2008.04.018. Epub 2008 May 2.


Development and partial characterization of high-affinity monoclonal antibodies for botulinum toxin type A and their use in analysis of milk by sandwich ELISA.

Stanker LH, Merrill P, Scotcher MC, Cheng LW.

J Immunol Methods. 2008 Jul 20;336(1):1-8. doi: 10.1016/j.jim.2008.03.003. Epub 2008 Apr 9.


The complete genome sequence and analysis of the epsilonproteobacterium Arcobacter butzleri.

Miller WG, Parker CT, Rubenfield M, Mendz GL, Wösten MM, Ussery DW, Stolz JF, Binnewies TT, Hallin PF, Wang G, Malek JA, Rogosin A, Stanker LH, Mandrell RE.

PLoS One. 2007 Dec 26;2(12):e1358.


Survival of Escherichia coli O157:H7 in wastewater from dairy lagoons.

Ravva SV, Sarreal CZ, Duffy B, Stanker LH.

J Appl Microbiol. 2006 Oct;101(4):891-902.


Prevalence of Campylobacter spp isolated from the intestinal tract of pigs raised in an integrated swine production system.

Harvey RB, Young CR, Ziprin RL, Hume ME, Genovese KJ, Anderson RC, Droleskey RE, Stanker LH, Nisbet DJ.

J Am Vet Med Assoc. 1999 Dec 1;215(11):1601-4.


Fluorescent marker for the detection of crop and upper gastrointestinal leakage in poultry processing plants.

Byrd JA, Hargis BM, Corrier DE, Brewer RL, Caldwell DJ, Bailey RH, McReynolds JL, Herron KL, Stanker LH.

Poult Sci. 2002 Jan;81(1):70-4.


Chicken mim-1 protein, P33, is a heterophil chemotactic factor present in Salmonella enteritidis immune lymphokine.

Bischoff KM, Pishko EJ, Genovese KJ, Crippen TL, Holtzapple CK, Stanker LH, Nisbet DJ, Kogut MH.

J Food Prot. 2001 Oct;64(10):1503-9.


Role of Campylobacter jejuni potential virulence genes in cecal colonization.

Ziprin RL, Young CR, Byrd JA, Stanker LH, Hume ME, Gray SA, Kim BJ, Konkel ME.

Avian Dis. 2001 Jul-Sep;45(3):549-57.


Genotypic variation among arcobacter isolates from a farrow-to-finish swine facility.

Hume ME, Harvey RB, Stanker LH, Droleskey RE, Poole TL, Zhang HB.

J Food Prot. 2001 May;64(5):645-51.


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