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β-Cardiac myosin hypertrophic cardiomyopathy mutations release sequestered heads and increase enzymatic activity.

Adhikari AS, Trivedi DV, Sarkar SS, Song D, Kooiker KB, Bernstein D, Spudich JA, Ruppel KM.

Nat Commun. 2019 Jun 18;10(1):2685. doi: 10.1038/s41467-019-10555-9.


Three perspectives on the molecular basis of hypercontractility caused by hypertrophic cardiomyopathy mutations.

Spudich JA.

Pflugers Arch. 2019 May;471(5):701-717. doi: 10.1007/s00424-019-02259-2. Epub 2019 Feb 15. Review.


SETD3 is an actin histidine methyltransferase that prevents primary dystocia.

Wilkinson AW, Diep J, Dai S, Liu S, Ooi YS, Song D, Li TM, Horton JR, Zhang X, Liu C, Trivedi DV, Ruppel KM, Vilches-Moure JG, Casey KM, Mak J, Cowan T, Elias JE, Nagamine CM, Spudich JA, Cheng X, Carette JE, Gozani O.

Nature. 2019 Jan;565(7739):372-376. doi: 10.1038/s41586-018-0821-8. Epub 2018 Dec 10.


Deciphering the super relaxed state of human β-cardiac myosin and the mode of action of mavacamten from myosin molecules to muscle fibers.

Anderson RL, Trivedi DV, Sarkar SS, Henze M, Ma W, Gong H, Rogers CS, Gorham JM, Wong FL, Morck MM, Seidman JG, Ruppel KM, Irving TC, Cooke R, Green EM, Spudich JA.

Proc Natl Acad Sci U S A. 2018 Aug 28;115(35):E8143-E8152. doi: 10.1073/pnas.1809540115. Epub 2018 Aug 13.


Controlling load-dependent kinetics of β-cardiac myosin at the single-molecule level.

Liu C, Kawana M, Song D, Ruppel KM, Spudich JA.

Nat Struct Mol Biol. 2018 Jun;25(6):505-514. doi: 10.1038/s41594-018-0069-x. Epub 2018 Jun 4.


Dilated cardiomyopathy myosin mutants have reduced force-generating capacity.

Ujfalusi Z, Vera CD, Mijailovich SM, Svicevic M, Yu EC, Kawana M, Ruppel KM, Spudich JA, Geeves MA, Leinwand LA.

J Biol Chem. 2018 Jun 8;293(23):9017-9029. doi: 10.1074/jbc.RA118.001938. Epub 2018 Apr 17.


Molecular mechanisms and structural features of cardiomyopathy-causing troponin T mutants in the tropomyosin overlap region.

Gangadharan B, Sunitha MS, Mukherjee S, Chowdhury RR, Haque F, Sekar N, Sowdhamini R, Spudich JA, Mercer JA.

Proc Natl Acad Sci U S A. 2017 Oct 17;114(42):11115-11120. doi: 10.1073/pnas.1710354114. Epub 2017 Oct 2.


Hypertrophic cardiomyopathy and the myosin mesa: viewing an old disease in a new light.

Trivedi DV, Adhikari AS, Sarkar SS, Ruppel KM, Spudich JA.

Biophys Rev. 2018 Feb;10(1):27-48. doi: 10.1007/s12551-017-0274-6. Epub 2017 Jul 17. Review.


The myosin mesa and the basis of hypercontractility caused by hypertrophic cardiomyopathy mutations.

Nag S, Trivedi DV, Sarkar SS, Adhikari AS, Sunitha MS, Sutton S, Ruppel KM, Spudich JA.

Nat Struct Mol Biol. 2017 Jun;24(6):525-533. doi: 10.1038/nsmb.3408. Epub 2017 May 8.


Biophysical properties of human β-cardiac myosin with converter mutations that cause hypertrophic cardiomyopathy.

Kawana M, Sarkar SS, Sutton S, Ruppel KM, Spudich JA.

Sci Adv. 2017 Feb 10;3(2):e1601959. doi: 10.1126/sciadv.1601959. eCollection 2017 Feb.


How to Measure Load-Dependent Kinetics of Individual Motor Molecules Without a Force-Clamp.

Sung J, Mortensen KI, Spudich JA, Flyvbjerg H.

Methods Enzymol. 2017;582:1-29. doi: 10.1016/bs.mie.2016.08.002. Epub 2016 Oct 31.


Early-Onset Hypertrophic Cardiomyopathy Mutations Significantly Increase the Velocity, Force, and Actin-Activated ATPase Activity of Human β-Cardiac Myosin.

Adhikari AS, Kooiker KB, Sarkar SS, Liu C, Bernstein D, Spudich JA, Ruppel KM.

Cell Rep. 2016 Dec 13;17(11):2857-2864. doi: 10.1016/j.celrep.2016.11.040.


How to Measure Separations and Angles Between Intramolecular Fluorescent Markers.

Mortensen KI, Sung J, Spudich JA, Flyvbjerg H.

Methods Enzymol. 2016;581:147-185. doi: 10.1016/bs.mie.2016.08.020. Epub 2016 Oct 6.


Multidimensional structure-function relationships in human β-cardiac myosin from population-scale genetic variation.

Homburger JR, Green EM, Caleshu C, Sunitha MS, Taylor RE, Ruppel KM, Metpally RP, Colan SD, Michels M, Day SM, Olivotto I, Bustamante CD, Dewey FE, Ho CY, Spudich JA, Ashley EA.

Proc Natl Acad Sci U S A. 2016 Jun 14;113(24):6701-6. doi: 10.1073/pnas.1606950113. Epub 2016 May 31.


A small-molecule inhibitor of sarcomere contractility suppresses hypertrophic cardiomyopathy in mice.

Green EM, Wakimoto H, Anderson RL, Evanchik MJ, Gorham JM, Harrison BC, Henze M, Kawas R, Oslob JD, Rodriguez HM, Song Y, Wan W, Leinwand LA, Spudich JA, McDowell RS, Seidman JG, Seidman CE.

Science. 2016 Feb 5;351(6273):617-21. doi: 10.1126/science.aad3456.


Effects of hypertrophic and dilated cardiomyopathy mutations on power output by human β-cardiac myosin.

Spudich JA, Aksel T, Bartholomew SR, Nag S, Kawana M, Yu EC, Sarkar SS, Sung J, Sommese RF, Sutton S, Cho C, Adhikari AS, Taylor R, Liu C, Trivedi D, Ruppel KM.

J Exp Biol. 2016 Jan;219(Pt 2):161-7. doi: 10.1242/jeb.125930. Review.


Contractility parameters of human β-cardiac myosin with the hypertrophic cardiomyopathy mutation R403Q show loss of motor function.

Nag S, Sommese RF, Ujfalusi Z, Combs A, Langer S, Sutton S, Leinwand LA, Geeves MA, Ruppel KM, Spudich JA.

Sci Adv. 2015 Oct 9;1(9):e1500511. doi: 10.1126/sciadv.1500511. eCollection 2015 Oct.


Optimized measurements of separations and angles between intra-molecular fluorescent markers.

Mortensen KI, Sung J, Flyvbjerg H, Spudich JA.

Nat Commun. 2015 Oct 16;6:8621. doi: 10.1038/ncomms9621.


Establishing disease causality for a novel gene variant in familial dilated cardiomyopathy using a functional in-vitro assay of regulated thin filaments and human cardiac myosin.

Pan S, Sommese RF, Sallam KI, Nag S, Sutton S, Miller SM, Spudich JA, Ruppel KM, Ashley EA.

BMC Med Genet. 2015 Oct 26;16:97. doi: 10.1186/s12881-015-0243-5.


Harmonic force spectroscopy measures load-dependent kinetics of individual human β-cardiac myosin molecules.

Sung J, Nag S, Mortensen KI, Vestergaard CL, Sutton S, Ruppel K, Flyvbjerg H, Spudich JA.

Nat Commun. 2015 Aug 4;6:7931. doi: 10.1038/ncomms8931.


Mechanical coordination in motor ensembles revealed using engineered artificial myosin filaments.

Hariadi RF, Sommese RF, Adhikari AS, Taylor RE, Sutton S, Spudich JA, Sivaramakrishnan S.

Nat Nanotechnol. 2015 Aug;10(8):696-700. doi: 10.1038/nnano.2015.132. Epub 2015 Jul 6.


Ensemble force changes that result from human cardiac myosin mutations and a small-molecule effector.

Aksel T, Choe Yu E, Sutton S, Ruppel KM, Spudich JA.

Cell Rep. 2015 May 12;11(6):910-920. doi: 10.1016/j.celrep.2015.04.006. Epub 2015 Apr 30.


Mechanistic heterogeneity in contractile properties of α-tropomyosin (TPM1) mutants associated with inherited cardiomyopathies.

Gupte TM, Haque F, Gangadharan B, Sunitha MS, Mukherjee S, Anandhan S, Rani DS, Mukundan N, Jambekar A, Thangaraj K, Sowdhamini R, Sommese RF, Nag S, Spudich JA, Mercer JA.

J Biol Chem. 2015 Mar 13;290(11):7003-15. doi: 10.1074/jbc.M114.596676. Epub 2014 Dec 29.


Effects of troponin T cardiomyopathy mutations on the calcium sensitivity of the regulated thin filament and the actomyosin cross-bridge kinetics of human β-cardiac myosin.

Sommese RF, Nag S, Sutton S, Miller SM, Spudich JA, Ruppel KM.

PLoS One. 2013 Dec 18;8(12):e83403. doi: 10.1371/journal.pone.0083403. eCollection 2013.


Molecular consequences of the R453C hypertrophic cardiomyopathy mutation on human β-cardiac myosin motor function.

Sommese RF, Sung J, Nag S, Sutton S, Deacon JC, Choe E, Leinwand LA, Ruppel K, Spudich JA.

Proc Natl Acad Sci U S A. 2013 Jul 30;110(31):12607-12. doi: 10.1073/pnas.1309493110. Epub 2013 Jun 24.


Single-molecule fluorescence imaging of processive myosin with enhanced background suppression using linear zero-mode waveguides (ZMWs) and convex lens induced confinement (CLIC).

Elting MW, Leslie SR, Churchman LS, Korlach J, McFaul CM, Leith JS, Levene MJ, Cohen AE, Spudich JA.

Opt Express. 2013 Jan 14;21(1):1189-202. doi: 10.1364/OE.21.001189.


Future challenges in single-molecule fluorescence and laser trap approaches to studies of molecular motors.

Elting MW, Spudich JA.

Dev Cell. 2012 Dec 11;23(6):1084-91. doi: 10.1016/j.devcel.2012.10.002. Review.


Integrative structural modelling of the cardiac thin filament: energetics at the interface and conservation patterns reveal a spotlight on period 2 of tropomyosin.

Margaret Sunitha S, Mercer JA, Spudich JA, Sowdhamini R.

Bioinform Biol Insights. 2012;6:203-23. doi: 10.4137/BBI.S9798. Epub 2012 Oct 3.


One path to understanding energy transduction in biological systems.

Spudich JA.

Nat Med. 2012 Oct;18(10):1478-82. doi: 10.1038/nm.2924. No abstract available.


Cell-intrinsic functional effects of the α-cardiac myosin Arg-403-Gln mutation in familial hypertrophic cardiomyopathy.

Chuan P, Sivaramakrishnan S, Ashley EA, Spudich JA.

Biophys J. 2012 Jun 20;102(12):2782-90. doi: 10.1016/j.bpj.2012.04.049. Epub 2012 Jun 19.


The myosin superfamily at a glance.

Hartman MA, Spudich JA.

J Cell Sci. 2012 Apr 1;125(Pt 7):1627-32. doi: 10.1242/jcs.094300. Review. No abstract available.


Structural and functional insights on the Myosin superfamily.

Syamaladevi DP, Spudich JA, Sowdhamini R.

Bioinform Biol Insights. 2012;6:11-21. doi: 10.4137/BBI.S8451. Epub 2012 Feb 1.


Single-molecule high-resolution colocalization of single probes.

Churchman LS, Spudich JA.

Cold Spring Harb Protoc. 2012 Feb 1;2012(2):242-5. doi: 10.1101/pdb.prot067926.


Colocalization of fluorescent probes: accurate and precise registration with nanometer resolution.

Churchman LS, Spudich JA.

Cold Spring Harb Protoc. 2012 Feb 1;2012(2):141-9. doi: 10.1101/pdb.top067918.


Single-molecule gold-nanoparticle tracking.

Dunn AR, Spudich JA.

Cold Spring Harb Protoc. 2011 Dec 1;2011(12):1498-506. doi: 10.1101/pdb.prot066977.


Systematic control of protein interaction using a modular ER/K α-helix linker.

Sivaramakrishnan S, Spudich JA.

Proc Natl Acad Sci U S A. 2011 Dec 20;108(51):20467-72. doi: 10.1073/pnas.1116066108. Epub 2011 Nov 28.


The optical trapping dumbbell assay for nonprocessive motors or motors that turn around filaments.

Spudich JA, Rice SE, Rock RS, Purcell TJ, Warrick HM.

Cold Spring Harb Protoc. 2011 Nov 1;2011(11):1372-4. doi: 10.1101/pdb.prot066688.


Attachment of anti-GFP antibodies to microspheres for optical trapping experiments.

Spudich JA, Rice SE, Rock RS, Purcell TJ, Warrick HM.

Cold Spring Harb Protoc. 2011 Nov 1;2011(11):1370-1. doi: 10.1101/pdb.prot066670.


Optical traps to study properties of molecular motors.

Spudich JA, Rice SE, Rock RS, Purcell TJ, Warrick HM.

Cold Spring Harb Protoc. 2011 Nov 1;2011(11):1305-18. doi: 10.1101/pdb.top066662.


Molecular motors: forty years of interdisciplinary research.

Spudich JA.

Mol Biol Cell. 2011 Nov;22(21):3936-9. doi: 10.1091/mbc.E11-05-0447.


Principles of unconventional myosin function and targeting.

Hartman MA, Finan D, Sivaramakrishnan S, Spudich JA.

Annu Rev Cell Dev Biol. 2011;27:133-55. doi: 10.1146/annurev-cellbio-100809-151502. Epub 2011 May 31. Review.


Biochemistry. Molecular motors, beauty in complexity.

Spudich JA.

Science. 2011 Mar 4;331(6021):1143-4. doi: 10.1126/science.1203978. No abstract available.


Proteomics approach to study the functions of Drosophila myosin VI through identification of multiple cargo-binding proteins.

Finan D, Hartman MA, Spudich JA.

Proc Natl Acad Sci U S A. 2011 Apr 5;108(14):5566-71. doi: 10.1073/pnas.1101415108. Epub 2011 Feb 28.


Detailed tuning of structure and intramolecular communication are dispensable for processive motion of myosin VI.

Elting MW, Bryant Z, Liao JC, Spudich JA.

Biophys J. 2011 Jan 19;100(2):430-9. doi: 10.1016/j.bpj.2010.11.045.


Nucleotide pocket thermodynamics measured by EPR reveal how energy partitioning relates myosin speed to efficiency.

Purcell TJ, Naber N, Franks-Skiba K, Dunn AR, Eldred CC, Berger CL, Málnási-Csizmadia A, Spudich JA, Swank DM, Pate E, Cooke R.

J Mol Biol. 2011 Mar 18;407(1):79-91. doi: 10.1016/j.jmb.2010.11.053. Epub 2010 Dec 23.


Robust mechanosensing and tension generation by myosin VI.

Chuan P, Spudich JA, Dunn AR.

J Mol Biol. 2011 Jan 7;405(1):105-12. doi: 10.1016/j.jmb.2010.10.010. Epub 2010 Oct 21.


Helicity of short E-R/K peptides.

Sommese RF, Sivaramakrishnan S, Baldwin RL, Spudich JA.

Protein Sci. 2010 Oct;19(10):2001-5. doi: 10.1002/pro.469.


Single-molecule dual-beam optical trap analysis of protein structure and function.

Sung J, Sivaramakrishnan S, Dunn AR, Spudich JA.

Methods Enzymol. 2010;475:321-75. doi: 10.1016/S0076-6879(10)75014-X.


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