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Items: 50

1.

A transporter converted into a sensor, a phototaxis signaling mutant of bacteriorhodopsin at 3.0 Å.

Spudich EN, Ozorowski G, Schow EV, Tobias DJ, Spudich JL, Luecke H.

J Mol Biol. 2012 Jan 20;415(3):455-63. doi: 10.1016/j.jmb.2011.11.025. Epub 2011 Nov 22.

2.

Diversity of Chlamydomonas channelrhodopsins.

Hou SY, Govorunova EG, Ntefidou M, Lane CE, Spudich EN, Sineshchekov OA, Spudich JL.

Photochem Photobiol. 2012 Jan-Feb;88(1):119-28. doi: 10.1111/j.1751-1097.2011.01027.x. Epub 2011 Nov 29.

3.

New channelrhodopsin with a red-shifted spectrum and rapid kinetics from Mesostigma viride.

Govorunova EG, Spudich EN, Lane CE, Sineshchekov OA, Spudich JL.

MBio. 2011 Jun 21;2(3):e00115-11. doi: 10.1128/mBio.00115-11. Print 2011.

4.

Active water in protein-protein communication within the membrane: the case of SRII-HtrII signal relay.

Bergo VB, Spudich EN, Spudich JL, Rothschild KJ.

Biochemistry. 2009 Feb 10;48(5):811-3. doi: 10.1021/bi802180a.

5.

His-75 in proteorhodopsin, a novel component in light-driven proton translocation by primary pumps.

Bergo VB, Sineshchekov OA, Kralj JM, Partha R, Spudich EN, Rothschild KJ, Spudich JL.

J Biol Chem. 2009 Jan 30;284(5):2836-43. doi: 10.1074/jbc.M803792200. Epub 2008 Nov 17.

6.

Different structural changes occur in blue- and green-proteorhodopsins during the primary photoreaction.

Amsden JJ, Kralj JM, Bergo VB, Spudich EN, Spudich JL, Rothschild KJ.

Biochemistry. 2008 Nov 4;47(44):11490-8. doi: 10.1021/bi800945t. Epub 2008 Oct 9.

7.

Raman spectroscopy reveals direct chromophore interactions in the Leu/Gln105 spectral tuning switch of proteorhodopsins.

Kralj JM, Spudich EN, Spudich JL, Rothschild KJ.

J Phys Chem B. 2008 Sep 18;112(37):11770-6. doi: 10.1021/jp802629e. Epub 2008 Aug 22.

8.

Protonation state of Glu142 differs in the green- and blue-absorbing variants of proteorhodopsin.

Kralj JM, Bergo VB, Amsden JJ, Spudich EN, Spudich JL, Rothschild KJ.

Biochemistry. 2008 Mar 18;47(11):3447-53. doi: 10.1021/bi7018964. Epub 2008 Feb 20.

PMID:
18284210
9.

Constitutive activity in chimeras and deletions localize sensory rhodopsin II/HtrII signal relay to the membrane-inserted domain.

Sasaki J, Nara T, Spudich EN, Spudich JL.

Mol Microbiol. 2007 Dec;66(6):1321-30. Epub 2007 Nov 6.

10.

Subpicosecond protein backbone changes detected during the green-absorbing proteorhodopsin primary photoreaction.

Amsden JJ, Kralj JM, Chieffo LR, Wang X, Erramilli S, Spudich EN, Spudich JL, Ziegler LD, Rothschild KJ.

J Phys Chem B. 2007 Oct 11;111(40):11824-31. Epub 2007 Sep 19.

PMID:
17880126
11.

Crystal structure of the Anabaena sensory rhodopsin transducer.

Vogeley L, Trivedi VD, Sineshchekov OA, Spudich EN, Spudich JL, Luecke H.

J Mol Biol. 2007 Mar 30;367(3):741-51. Epub 2006 Dec 1.

12.

Role of the cytoplasmic domain in Anabaena sensory rhodopsin photocycling: vectoriality of Schiff base deprotonation.

Sineshchekov OA, Spudich EN, Trivedi VD, Spudich JL.

Biophys J. 2006 Dec 15;91(12):4519-27. Epub 2006 Sep 29.

13.

Photoactivation perturbs the membrane-embedded contacts between sensory rhodopsin II and its transducer.

Bergo VB, Spudich EN, Rothschild KJ, Spudich JL.

J Biol Chem. 2005 Aug 5;280(31):28365-9. Epub 2005 Jun 10.

14.

The cytoplasmic membrane-proximal domain of the HtrII transducer interacts with the E-F loop of photoactivated Natronomonas pharaonis sensory rhodopsin II.

Yang CS, Sineshchekov O, Spudich EN, Spudich JL.

J Biol Chem. 2004 Oct 8;279(41):42970-6. Epub 2004 Jul 15. Erratum in: J Biol Chem. 2005 May 27;280(21):20915.

15.

Structural changes in the photoactive site of proteorhodopsin during the primary photoreaction.

Bergo V, Amsden JJ, Spudich EN, Spudich JL, Rothschild KJ.

Biochemistry. 2004 Jul 20;43(28):9075-83.

PMID:
15248764
16.

Characterization of RS29, a blue-green proteorhodopsin variant from the Red Sea.

Man-Aharonovich D, Sabehi G, Sineshchekov OA, Spudich EN, Spudich JL, Béjà O.

Photochem Photobiol Sci. 2004 May;3(5):459-62. Epub 2004 Mar 31.

PMID:
15122363
17.

Conformational changes detected in a sensory rhodopsin II-transducer complex.

Bergo V, Spudich EN, Spudich JL, Rothschild KJ.

J Biol Chem. 2003 Sep 19;278(38):36556-62. Epub 2003 Jun 23.

18.

Spectroscopic and photochemical characterization of a deep ocean proteorhodopsin.

Wang WW, Sineshchekov OA, Spudich EN, Spudich JL.

J Biol Chem. 2003 Sep 5;278(36):33985-91. Epub 2003 Jun 23.

19.

Diversification and spectral tuning in marine proteorhodopsins.

Man D, Wang W, Sabehi G, Aravind L, Post AF, Massana R, Spudich EN, Spudich JL, Béjà O.

EMBO J. 2003 Apr 15;22(8):1725-31.

20.

A Fourier transform infrared study of Neurospora rhodopsin: similarities with archaeal rhodopsins.

Bergo V, Spudich EN, Spudich JL, Rothschild KJ.

Photochem Photobiol. 2002 Sep;76(3):341-9.

PMID:
12403457
21.

Proton transfers in the photochemical reaction cycle of proteorhodopsin.

Dioumaev AK, Brown LS, Shih J, Spudich EN, Spudich JL, Lanyi JK.

Biochemistry. 2002 Apr 30;41(17):5348-58.

PMID:
11969395
22.

An archaeal photosignal-transducing module mediates phototaxis in Escherichia coli.

Jung KH, Spudich EN, Trivedi VD, Spudich JL.

J Bacteriol. 2001 Nov;183(21):6365-71.

23.

Proteorhodopsin phototrophy in the ocean.

Béjà O, Spudich EN, Spudich JL, Leclerc M, DeLong EF.

Nature. 2001 Jun 14;411(6839):786-9.

PMID:
11459054
24.

Crystal structure of sensory rhodopsin II at 2.4 angstroms: insights into color tuning and transducer interaction.

Luecke H, Schobert B, Lanyi JK, Spudich EN, Spudich JL.

Science. 2001 Aug 24;293(5534):1499-503. Epub 2001 Jul 12.

25.

Photochemical reaction cycle and proton transfers in Neurospora rhodopsin.

Brown LS, Dioumaev AK, Lanyi JK, Spudich EN, Spudich JL.

J Biol Chem. 2001 Aug 31;276(35):32495-505. Epub 2001 Jul 2.

26.

Electron crystallographic analysis of two-dimensional crystals of sensory rhodopsin II: a 6.9 A projection structure.

Kunji ER, Spudich EN, Grisshammer R, Henderson R, Spudich JL.

J Mol Biol. 2001 Apr 27;308(2):279-93.

PMID:
11327767
27.

Retinylidene proteins: structures and functions from archaea to humans.

Spudich JL, Yang CS, Jung KH, Spudich EN.

Annu Rev Cell Dev Biol. 2000;16:365-92. Review.

PMID:
11031241
28.

Bacterial rhodopsin: evidence for a new type of phototrophy in the sea.

Béjà O, Aravind L, Koonin EV, Suzuki MT, Hadd A, Nguyen LP, Jovanovich SB, Gates CM, Feldman RA, Spudich JL, Spudich EN, DeLong EF.

Science. 2000 Sep 15;289(5486):1902-6.

29.

FTIR analysis of the SII540 intermediate of sensory rhodopsin II: Asp73 is the Schiff base proton acceptor.

Bergo V, Spudich EN, Scott KL, Spudich JL, Rothschild KJ.

Biochemistry. 2000 Mar 21;39(11):2823-30.

PMID:
10715101
30.

A eukaryotic protein, NOP-1, binds retinal to form an archaeal rhodopsin-like photochemically reactive pigment.

Bieszke JA, Spudich EN, Scott KL, Borkovich KA, Spudich JL.

Biochemistry. 1999 Oct 26;38(43):14138-45.

PMID:
10571987
31.

Transducer-binding and transducer-mutations modulate photoactive-site-deprotonation in sensory rhodopsin I.

Jung KH, Spudich EN, Dag P, Spudich JL.

Biochemistry. 1999 Oct 5;38(40):13270-4.

PMID:
10529200
32.

Effects of substitutions D73E, D73N, D103N and V106M on signaling and pH titration of sensory rhodopsin II.

Zhu J, Spudich EN, Alam M, Spudich JL.

Photochem Photobiol. 1997 Dec;66(6):788-91.

PMID:
9421965
33.
34.

Deletion mapping of the sites on the HtrI transducer for sensory rhodopsin I interaction.

Perazzona B, Spudich EN, Spudich JL.

J Bacteriol. 1996 Nov;178(22):6475-8.

35.

Rapid high-yield purification and liposome reconstitution of polyhistidine-tagged sensory rhodopsin I.

Krebs MP, Spudich EN, Spudich JL.

Protein Expr Purif. 1995 Dec;6(6):780-8.

PMID:
8746630
36.
37.

The photochemical reactions of sensory rhodopsin I are altered by its transducer.

Spudich EN, Spudich JL.

J Biol Chem. 1993 Aug 5;268(22):16095-7.

38.

Synthesis of a gene for sensory rhodopsin I and its functional expression in Halobacterium halobium.

Krebs MP, Spudich EN, Khorana HG, Spudich JL.

Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3486-90.

39.

Conformational changes in sensory rhodopsin I: similarities and differences with bacteriorhodopsin, halorhodopsin, and rhodopsin.

Bousché O, Spudich EN, Spudich JL, Rothschild KJ.

Biochemistry. 1991 Jun 4;30(22):5395-400.

PMID:
2036407
40.

Sensory rhodopsins I and II modulate a methylation/demethylation system in Halobacterium halobium phototaxis.

Spudich EN, Takahashi T, Spudich JL.

Proc Natl Acad Sci U S A. 1989 Oct;86(20):7746-50.

41.

Methyl-accepting protein associated with bacterial sensory rhodopsin I.

Spudich EN, Hasselbacher CA, Spudich JL.

J Bacteriol. 1988 Sep;170(9):4280-5.

42.

Properties of a second sensory receptor protein in Halobacterium halobium phototaxis.

Spudich EN, Sundberg SA, Manor D, Spudich JL.

Proteins. 1986 Nov;1(3):239-46.

PMID:
3449857
43.

Biochemical characterization of halorhodopsin in native membranes.

Spudich EN, Spudich JL.

J Biol Chem. 1985 Jan 25;260(2):1208-12.

45.

Genetic and biochemical resolution of the chromophoric polypeptide of halorhodopsin.

Spudich EN, Bogomolni RA, Spudich JL.

Biochem Biophys Res Commun. 1983 Apr 15;112(1):332-8.

PMID:
6838616
48.
49.

Fine-structure map of the histidine transport genes in Salmonella typhimurium.

Ames GF, Noel KD, Taber H, Spudich EN, Nikaido K, Afong J.

J Bacteriol. 1977 Mar;129(3):1289-97.

50.

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