Format

Send to

Choose Destination
Development. 2019 Jun 21;146(13). pii: dev176305. doi: 10.1242/dev.176305.

Specification of basal region identity after asymmetric zygotic division requires mitogen-activated protein kinase 6 in rice.

Author information

1
Department of Plant Production Sciences, Graduate School of Bioagricultural sciences, Nagoya University, Nagoya, Aichi 464-8601, Japan.
2
Molecular Genetics Department, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
3
Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan.
4
Genome Resource Unit, Agrogenomics Resource Center, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
5
National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan.
6
College of Bioscience and Biotechnology, Chubu University, Kasugai, Aichi 487-8501, Japan.
7
National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan yusato@nig.ac.jp.

Abstract

Asymmetric cell division is a key step in cellular differentiation in multicellular organisms. In plants, asymmetric zygotic division produces the apical and basal cells. The mitogen-activated protein kinase (MPK) cascade in Arabidopsis acts in asymmetric divisions such as zygotic division and stomatal development, but whether the effect on cellular differentiation of this cascade is direct or indirect following asymmetric division is not clear. Here, we report the analysis of a rice mutant, globular embryo 4 (gle4). In two- and four-cell-stage embryos, asymmetric zygotic division and subsequent cell division patterns were indistinguishable between the wild type and gle4 mutants. However, marker gene expression and transcriptome analyses showed that specification of the basal region was compromised in gle4 We found that GLE4 encodes MPK6 and that GLE4/MPK6 is essential in cellular differentiation rather than in asymmetric zygotic division. Our findings provide a new insight into the role of MPK in plant development. We propose that the regulation of asymmetric zygotic division is separate from the regulation of cellular differentiation that leads to apical-basal polarity.

KEYWORDS:

Embryogenesis; GLE4; GLOBULAR EMBRYO 4; MPK6; Rice

PMID:
31118231
DOI:
10.1242/dev.176305

Conflict of interest statement

Competing interestsThe authors declare no competing or financial interests.

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center