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Items: 36

1.

Measuring the Activities of Two-Component Regulatory System Phosphatases.

Bourret RB, Silversmith RE.

Methods Enzymol. 2018;607:321-351. doi: 10.1016/bs.mie.2018.05.006. Epub 2018 Jun 23.

PMID:
30149864
2.

Fluorescence Measurement of Kinetics of CheY Autophosphorylation with Small Molecule Phosphodonors.

Silversmith RE, Bourret RB.

Methods Mol Biol. 2018;1729:321-335. doi: 10.1007/978-1-4939-7577-8_25.

PMID:
29429101
3.

A Variable Active Site Residue Influences the Kinetics of Response Regulator Phosphorylation and Dephosphorylation.

Immormino RM, Silversmith RE, Bourret RB.

Biochemistry. 2016 Oct 4;55(39):5595-5609. Epub 2016 Sep 19.

4.

Phosphoryl Group Flow within the Pseudomonas aeruginosa Pil-Chp Chemosensory System: DIFFERENTIAL FUNCTION OF THE EIGHT PHOSPHOTRANSFERASE AND THREE RECEIVER DOMAINS.

Silversmith RE, Wang B, Fulcher NB, Wolfgang MC, Bourret RB.

J Biol Chem. 2016 Aug 19;291(34):17677-91. doi: 10.1074/jbc.M116.737528. Epub 2016 Jun 27.

5.

Imidazole as a Small Molecule Analogue in Two-Component Signal Transduction.

Page SC, Silversmith RE, Collins EJ, Bourret RB.

Biochemistry. 2015 Dec 15;54(49):7248-60. doi: 10.1021/acs.biochem.5b01082. Epub 2015 Dec 2.

6.

Probing Mechanistic Similarities between Response Regulator Signaling Proteins and Haloacid Dehalogenase Phosphatases.

Immormino RM, Starbird CA, Silversmith RE, Bourret RB.

Biochemistry. 2015 Jun 9;54(22):3514-27. doi: 10.1021/acs.biochem.5b00286. Epub 2015 May 28.

7.

A link between dimerization and autophosphorylation of the response regulator PhoB.

Creager-Allen RL, Silversmith RE, Bourret RB.

J Biol Chem. 2013 Jul 26;288(30):21755-69. doi: 10.1074/jbc.M113.471763. Epub 2013 Jun 11.

8.

Nonconserved active site residues modulate CheY autophosphorylation kinetics and phosphodonor preference.

Thomas SA, Immormino RM, Bourret RB, Silversmith RE.

Biochemistry. 2013 Apr 2;52(13):2262-73. doi: 10.1021/bi301654m. Epub 2013 Mar 19.

9.
10.

Measurement of response regulator autodephosphorylation rates spanning six orders of magnitude.

Bourret RB, Thomas SA, Page SC, Creager-Allen RL, Moore AM, Silversmith RE.

Methods Enzymol. 2010;471:89-114. doi: 10.1016/S0076-6879(10)71006-5. Epub 2010 Mar 1.

PMID:
20946844
11.

Two-component signal transduction.

Bourret RB, Silversmith RE.

Curr Opin Microbiol. 2010 Apr;13(2):113-5. doi: 10.1016/j.mib.2010.02.003. Epub 2010 Mar 10. No abstract available.

12.

Auxiliary phosphatases in two-component signal transduction.

Silversmith RE.

Curr Opin Microbiol. 2010 Apr;13(2):177-83. doi: 10.1016/j.mib.2010.01.004. Epub 2010 Feb 3. Review.

13.

Identical phosphatase mechanisms achieved through distinct modes of binding phosphoprotein substrate.

Pazy Y, Motaleb MA, Guarnieri MT, Charon NW, Zhao R, Silversmith RE.

Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):1924-9. doi: 10.1073/pnas.0911185107. Epub 2010 Jan 14.

14.

Matching biochemical reaction kinetics to the timescales of life: structural determinants that influence the autodephosphorylation rate of response regulator proteins.

Pazy Y, Wollish AC, Thomas SA, Miller PJ, Collins EJ, Bourret RB, Silversmith RE.

J Mol Biol. 2009 Oct 9;392(5):1205-20. doi: 10.1016/j.jmb.2009.07.064. Epub 2009 Jul 28.

15.

Kinetic characterization of catalysis by the chemotaxis phosphatase CheZ. Modulation of activity by the phosphorylated CheY substrate.

Silversmith RE, Levin MD, Schilling E, Bourret RB.

J Biol Chem. 2008 Jan 11;283(2):756-65. Epub 2007 Nov 12.

16.

CheX is a phosphorylated CheY phosphatase essential for Borrelia burgdorferi chemotaxis.

Motaleb MA, Miller MR, Li C, Bakker RG, Goldstein SF, Silversmith RE, Bourret RB, Charon NW.

J Bacteriol. 2005 Dec;187(23):7963-9.

17.

Sense and sensibility in bacteria. VIIIth International Conference on Bacterial Locomotion and Sensory Transduction.

Jenal U, Silversmith RE, Sogaard-Andersen L, Sockett L.

EMBO Rep. 2005 Jul;6(7):615-9. No abstract available.

19.

YbiV from Escherichia coli K12 is a HAD phosphatase.

Roberts A, Lee SY, McCullagh E, Silversmith RE, Wemmer DE.

Proteins. 2005 Mar 1;58(4):790-801.

PMID:
15657928
20.

A search for amino acid substitutions that universally activate response regulators.

Smith JG, Latiolais JA, Guanga GP, Pennington JD, Silversmith RE, Bourret RB.

Mol Microbiol. 2004 Feb;51(3):887-901.

21.

Investigation of the role of electrostatic charge in activation of the Escherichia coli response regulator CheY.

Smith JG, Latiolais JA, Guanga GP, Citineni S, Silversmith RE, Bourret RB.

J Bacteriol. 2003 Nov;185(21):6385-91.

22.

CheZ-mediated dephosphorylation of the Escherichia coli chemotaxis response regulator CheY: role for CheY glutamate 89.

Silversmith RE, Guanga GP, Betts L, Chu C, Zhao R, Bourret RB.

J Bacteriol. 2003 Mar;185(5):1495-502.

23.

Structure and catalytic mechanism of the E. coli chemotaxis phosphatase CheZ.

Zhao R, Collins EJ, Bourret RB, Silversmith RE.

Nat Struct Biol. 2002 Aug;9(8):570-5. Erratum in: Nat Struct Biol 2002 Sep;9(9):711.

PMID:
12080332
24.

Conformational coupling in the chemotaxis response regulator CheY.

Schuster M, Silversmith RE, Bourret RB.

Proc Natl Acad Sci U S A. 2001 May 22;98(11):6003-8. Epub 2001 May 15.

25.

Alteration of a nonconserved active site residue in the chemotaxis response regulator CheY affects phosphorylation and interaction with CheZ.

Silversmith RE, Smith JG, Guanga GP, Les JT, Bourret RB.

J Biol Chem. 2001 May 25;276(21):18478-84. Epub 2001 Feb 27.

26.

Isolation and characterization of nonchemotactic CheZ mutants of Escherichia coli.

Boesch KC, Silversmith RE, Bourret RB.

J Bacteriol. 2000 Jun;182(12):3544-52.

27.

Throwing the switch in bacterial chemotaxis.

Silversmith RE, Bourret RB.

Trends Microbiol. 1999 Jan;7(1):16-22. Review.

PMID:
10068993
28.

Computer-aided resolution of an experimental paradox in bacterial chemotaxis.

Abouhamad WN, Bray D, Schuster M, Boesch KC, Silversmith RE, Bourret RB.

J Bacteriol. 1998 Aug;180(15):3757-64.

29.
30.

Chemotactic response regulator mutant CheY95IV exhibits enhanced binding to the flagellar switch and phosphorylation-dependent constitutive signalling.

Schuster M, Abouhamad WN, Silversmith RE, Bourret RB.

Mol Microbiol. 1998 Mar;27(5):1065-75. Erratum in: Mol Microbiol 1998 May;28(4):863.

32.

T4-phage deoxycytidylate deaminase is a metalloprotein containing two zinc atoms per subunit.

Moore JT, Silversmith RE, Maley GF, Maley F.

J Biol Chem. 1993 Feb 5;268(4):2288-91.

33.
34.

Assembly of the membrane attack complex of complement on small unilamellar phospholipid vesicles.

Silversmith RE, Nelsestuen GL.

Biochemistry. 1986 Feb 25;25(4):852-60.

PMID:
3964649
35.

Fluid-phase assembly of the membrane attack complex of complement.

Silversmith RE, Nelsestuen GL.

Biochemistry. 1986 Feb 25;25(4):841-51.

PMID:
3964648
36.

Calcium effects on prothrombin and its reaction with bifunctional alkylating reagents.

Silversmith RE, Wei GJ, Nelsestuen GL.

Biochem Biophys Res Commun. 1983 Feb 28;111(1):213-8.

PMID:
6830590

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