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Biol Open. 2018 Oct 10. pii: bio.037978. doi: 10.1242/bio.037978. [Epub ahead of print]

Model-based serial blood sampling protocol for minimal mortality and better recovery in small to medium sized tilapia.

Author information

1
Department of Zoology, University of Johannesburg, Auckland Park, 2006, South Africa.
2
Department of Zoology, University of Johannesburg, Auckland Park, 2006, South Africa inaw@uj.ac.za.

Abstract

Serial blood sampling involving sampling blood from the same individual at different time points is essential in time-based studies including xenobiotic toxicokinetics and biochemical studies. However, high fish mortality due to phlebotomy-induced anaemia (PIA) constrains serial blood sampling in small to medium sized fish. The aim of the study was to develop and implement a model-based serial blood sampling protocol that minimizes fish mortality by regulating anaemia within levels that sustain fish survival and recovery. A model simulating the reduction in haemoglobin was developed from blood sampling data of sixteen (N=16) medium sized Oreochromis mossambicus The model was incorporated into a serial blood sampling protocol whose performance was tested on eight (N=8) fish. The protocol avoided fish mortality and the fish recovered from PIA within three weeks of the post-sampling period. Therefore, managing anaemia minimizes mortality and improves the applicability of serial blood sampling in small to medium sized fish.

KEYWORDS:

Fish; Model-based; Serial blood sampling

PMID:
30305283
DOI:
10.1242/bio.037978
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