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Items: 1 to 50 of 86

1.

A yeast display immunoprecipitation screen for targeted discovery of antibodies against membrane protein complexes.

Lajoie JM, Cho YK, Frost D, Bremner S, Li L, Shusta EV.

Protein Eng Des Sel. 2019 Nov 26. pii: gzz035. doi: 10.1093/protein/gzz035. [Epub ahead of print]

PMID:
31769480
2.

Correction to: An isogenic neurovascular unit model comprised of human induced pluripotent stem cell-derived brain microvascular endothelial cells, pericytes, astrocytes, and neurons.

Canfield SG, Stebbins MJ, Faubion MG, Gastfriend BD, Palecek SP, Shusta EV.

Fluids Barriers CNS. 2019 Sep 10;16(1):31. doi: 10.1186/s12987-019-0151-8.

3.

Streptococcus agalactiae disrupts P-glycoprotein function in brain endothelial cells.

Kim BJ, McDonagh MA, Deng L, Gastfriend BD, Schubert-Unkmeir A, Doran KS, Shusta EV.

Fluids Barriers CNS. 2019 Aug 22;16(1):26. doi: 10.1186/s12987-019-0146-5.

4.

An isogenic neurovascular unit model comprised of human induced pluripotent stem cell-derived brain microvascular endothelial cells, pericytes, astrocytes, and neurons.

Canfield SG, Stebbins MJ, Faubion MG, Gastfriend BD, Palecek SP, Shusta EV.

Fluids Barriers CNS. 2019 Aug 7;16(1):25. doi: 10.1186/s12987-019-0145-6. Erratum in: Fluids Barriers CNS. 2019 Sep 10;16(1):31.

5.

Past and Current Perspectives in Modeling Bacteria and Blood-Brain Barrier Interactions.

Kim BJ, Shusta EV, Doran KS.

Front Microbiol. 2019 Jun 13;10:1336. doi: 10.3389/fmicb.2019.01336. eCollection 2019.

6.

Induced Pluripotent Stem Cell-Derived Brain Endothelial Cells as a Cellular Model to Study Neisseria meningitidis Infection.

Martins Gomes SF, Westermann AJ, Sauerwein T, Hertlein T, Förstner KU, Ohlsen K, Metzger M, Shusta EV, Kim BJ, Appelt-Menzel A, Schubert-Unkmeir A.

Front Microbiol. 2019 May 29;10:1181. doi: 10.3389/fmicb.2019.01181. eCollection 2019.

7.

Regionally specified human pluripotent stem cell-derived astrocytes exhibit different molecular signatures and functional properties.

Bradley RA, Shireman J, McFalls C, Choi J, Canfield SG, Dong Y, Liu K, Lisota B, Jones JR, Petersen A, Bhattacharyya A, Palecek SP, Shusta EV, Kendziorski C, Zhang SC.

Development. 2019 Jul 8;146(13). pii: dev170910. doi: 10.1242/dev.170910.

8.

Identification of variable lymphocyte receptors that can target therapeutics to pathologically exposed brain extracellular matrix.

Umlauf BJ, Clark PA, Lajoie JM, Georgieva JV, Bremner S, Herrin BR, Kuo JS, Shusta EV.

Sci Adv. 2019 May 15;5(5):eaau4245. doi: 10.1126/sciadv.aau4245. eCollection 2019 May.

9.

Human pluripotent stem cell-derived brain pericyte-like cells induce blood-brain barrier properties.

Stebbins MJ, Gastfriend BD, Canfield SG, Lee MS, Richards D, Faubion MG, Li WJ, Daneman R, Palecek SP, Shusta EV.

Sci Adv. 2019 Mar 13;5(3):eaau7375. doi: 10.1126/sciadv.aau7375. eCollection 2019 Mar.

10.

Coupling Brain Perfusion Screens and Next Generation Sequencing to Identify Blood-Brain Barrier Binding Antibodies.

Stutz CC, Georgieva JV, Shusta EV.

AIChE J. 2018 Dec;64(12):4229-4236. doi: 10.1002/aic.16360. Epub 2018 Aug 22.

11.

Exploiting BBB disruption for the delivery of nanocarriers to the diseased CNS.

Umlauf BJ, Shusta EV.

Curr Opin Biotechnol. 2019 Dec;60:146-152. doi: 10.1016/j.copbio.2019.01.013. Epub 2019 Mar 5. Review. No abstract available.

PMID:
30849699
12.

Directed Differentiation of Human Pluripotent Stem Cells to Podocytes under Defined Conditions.

Qian T, Hernday SE, Bao X, Olson WR, Panzer SE, Shusta EV, Palecek SP.

Sci Rep. 2019 Feb 26;9(1):2765. doi: 10.1038/s41598-019-39504-8.

13.

Impacts of the -1 Amino Acid on Yeast Production of Protein-Intein Fusions.

Goulatis LI, Ramanathan R, Shusta EV.

Biotechnol Prog. 2019 Jan;35(1):e2736. doi: 10.1002/btpr.2736. Epub 2018 Nov 23.

PMID:
30341810
14.

Modeling the blood-brain barrier: Beyond the endothelial cells.

Gastfriend BD, Palecek SP, Shusta EV.

Curr Opin Biomed Eng. 2018 Mar;5:6-12. doi: 10.1016/j.cobme.2017.11.002.

15.

Site-Specific Antibody Functionalization Using Tetrazine-Styrene Cycloaddition.

Umlauf BJ, Mix KA, Grosskopf VA, Raines RT, Shusta EV.

Bioconjug Chem. 2018 May 16;29(5):1605-1613. doi: 10.1021/acs.bioconjchem.8b00114. Epub 2018 May 3.

16.

The variable lymphocyte receptor as an antibody alternative.

Waters EA, Shusta EV.

Curr Opin Biotechnol. 2018 Aug;52:74-79. doi: 10.1016/j.copbio.2018.02.016. Epub 2018 Mar 26. Review.

17.

Hyaluronan impairs the barrier integrity of brain microvascular endothelial cells through a CD44-dependent pathway.

Al-Ahmad AJ, Patel R, Palecek SP, Shusta EV.

J Cereb Blood Flow Metab. 2019 Sep;39(9):1759-1775. doi: 10.1177/0271678X18767748. Epub 2018 Mar 28.

PMID:
29589805
18.

Transthyretin Mimetics as Anti-β-Amyloid Agents: A Comparison of Peptide and Protein Approaches.

Pate KM, Kim BJ, Shusta EV, Murphy RM.

ChemMedChem. 2018 May 8;13(9):968-979. doi: 10.1002/cmdc.201800031. Epub 2018 Apr 16.

19.

Yeast display biopanning identifies human antibodies targeting glioblastoma stem-like cells.

Zorniak M, Clark PA, Umlauf BJ, Cho Y, Shusta EV, Kuo JS.

Sci Rep. 2017 Nov 20;7(1):15840. doi: 10.1038/s41598-017-16066-1.

20.

Directed differentiation of human pluripotent stem cells to blood-brain barrier endothelial cells.

Qian T, Maguire SE, Canfield SG, Bao X, Olson WR, Shusta EV, Palecek SP.

Sci Adv. 2017 Nov 8;3(11):e1701679. doi: 10.1126/sciadv.1701679. eCollection 2017 Nov.

21.

Modeling Group B Streptococcus and Blood-Brain Barrier Interaction by Using Induced Pluripotent Stem Cell-Derived Brain Endothelial Cells.

Kim BJ, Bee OB, McDonagh MA, Stebbins MJ, Palecek SP, Doran KS, Shusta EV.

mSphere. 2017 Nov 1;2(6). pii: e00398-17. doi: 10.1128/mSphere.00398-17. eCollection 2017 Nov-Dec.

22.

Activation of RARα, RARγ, or RXRα Increases Barrier Tightness in Human Induced Pluripotent Stem Cell-Derived Brain Endothelial Cells.

Stebbins MJ, Lippmann ES, Faubion MG, Daneman R, Palecek SP, Shusta EV.

Biotechnol J. 2018 Feb;13(2). doi: 10.1002/biot.201700093. Epub 2017 Sep 28.

23.

Modeling Psychomotor Retardation using iPSCs from MCT8-Deficient Patients Indicates a Prominent Role for the Blood-Brain Barrier.

Vatine GD, Al-Ahmad A, Barriga BK, Svendsen S, Salim A, Garcia L, Garcia VJ, Ho R, Yucer N, Qian T, Lim RG, Wu J, Thompson LM, Spivia WR, Chen Z, Van Eyk J, Palecek SP, Refetoff S, Shusta EV, Svendsen CN.

Cell Stem Cell. 2017 Jun 1;20(6):831-843.e5. doi: 10.1016/j.stem.2017.04.002. Epub 2017 May 16.

24.

Directed Evolution of Protein Thermal Stability Using Yeast Surface Display.

Traxlmayr MW, Shusta EV.

Methods Mol Biol. 2017;1575:45-65. doi: 10.1007/978-1-4939-6857-2_4.

PMID:
28255874
25.

Protein engineering approaches for regulating blood-brain barrier transcytosis.

Goulatis LI, Shusta EV.

Curr Opin Struct Biol. 2017 Aug;45:109-115. doi: 10.1016/j.sbi.2016.12.005. Epub 2016 Dec 29. Review.

PMID:
28040636
26.

An isogenic blood-brain barrier model comprising brain endothelial cells, astrocytes, and neurons derived from human induced pluripotent stem cells.

Canfield SG, Stebbins MJ, Morales BS, Asai SW, Vatine GD, Svendsen CN, Palecek SP, Shusta EV.

J Neurochem. 2017 Mar;140(6):874-888. doi: 10.1111/jnc.13923. Epub 2017 Feb 14.

27.
28.

Analysis of Cancer-Targeting Alkylphosphocholine Analogue Permeability Characteristics Using a Human Induced Pluripotent Stem Cell Blood-Brain Barrier Model.

Clark PA, Al-Ahmad AJ, Qian T, Zhang RR, Wilson HK, Weichert JP, Palecek SP, Kuo JS, Shusta EV.

Mol Pharm. 2016 Sep 6;13(9):3341-9. doi: 10.1021/acs.molpharmaceut.6b00441. Epub 2016 Aug 4.

29.

In vitro models of the blood-brain barrier: An overview of commonly used brain endothelial cell culture models and guidelines for their use.

Helms HC, Abbott NJ, Burek M, Cecchelli R, Couraud PO, Deli MA, Förster C, Galla HJ, Romero IA, Shusta EV, Stebbins MJ, Vandenhaute E, Weksler B, Brodin B.

J Cereb Blood Flow Metab. 2016 May;36(5):862-90. doi: 10.1177/0271678X16630991. Epub 2016 Feb 11. Review.

30.

Engineering an Anti-Transferrin Receptor ScFv for pH-Sensitive Binding Leads to Increased Intracellular Accumulation.

Tillotson BJ, Goulatis LI, Parenti I, Duxbury E, Shusta EV.

PLoS One. 2015 Dec 29;10(12):e0145820. doi: 10.1371/journal.pone.0145820. eCollection 2015.

31.

Pro-region engineering for improved yeast display and secretion of brain derived neurotrophic factor.

Burns ML, Malott TM, Metcalf KJ, Puguh A, Chan JR, Shusta EV.

Biotechnol J. 2016 Mar;11(3):425-36. doi: 10.1002/biot.201500360. Epub 2015 Dec 9.

PMID:
26580314
32.

Differentiation and characterization of human pluripotent stem cell-derived brain microvascular endothelial cells.

Stebbins MJ, Wilson HK, Canfield SG, Qian T, Palecek SP, Shusta EV.

Methods. 2016 May 15;101:93-102. doi: 10.1016/j.ymeth.2015.10.016. Epub 2015 Oct 27.

33.

Advances in microfluidic platforms for analyzing and regulating human pluripotent stem cells.

Qian T, Shusta EV, Palecek SP.

Curr Opin Genet Dev. 2015 Oct;34:54-60. doi: 10.1016/j.gde.2015.07.007. Epub 2015 Aug 24. Review.

34.

Yeast Display-Based Antibody Affinity Maturation Using Detergent-Solubilized Cell Lysates.

Tillotson BJ, Lajoie JM, Shusta EV.

Methods Mol Biol. 2015;1319:65-78. doi: 10.1007/978-1-4939-2748-7_4.

35.

Exploring the effects of cell seeding density on the differentiation of human pluripotent stem cells to brain microvascular endothelial cells.

Wilson HK, Canfield SG, Hjortness MK, Palecek SP, Shusta EV.

Fluids Barriers CNS. 2015 May 21;12:13. doi: 10.1186/s12987-015-0007-9.

36.

Efficient differentiation of human pluripotent stem cells to endothelial progenitors via small-molecule activation of WNT signaling.

Lian X, Bao X, Al-Ahmad A, Liu J, Wu Y, Dong W, Dunn KK, Shusta EV, Palecek SP.

Stem Cell Reports. 2014 Nov 11;3(5):804-16. doi: 10.1016/j.stemcr.2014.09.005. Epub 2014 Oct 9. Erratum in: Stem Cell Reports. 2015 Jan 13;4(1):170.

37.

An evolved Mxe GyrA intein for enhanced production of fusion proteins.

Marshall CJ, Grosskopf VA, Moehling TJ, Tillotson BJ, Wiepz GJ, Abbott NL, Raines RT, Shusta EV.

ACS Chem Biol. 2015 Feb 20;10(2):527-38. doi: 10.1021/cb500689g. Epub 2014 Nov 24.

38.

Introducing glycophage arrays: facile production, purification and patterning of glycophages.

Lajoie JM, Shusta EV.

Biotechnol J. 2015 Jan;10(1):20-1. doi: 10.1002/biot.201400591. Epub 2014 Oct 31.

PMID:
25359621
39.

Targeting receptor-mediated transport for delivery of biologics across the blood-brain barrier.

Lajoie JM, Shusta EV.

Annu Rev Pharmacol Toxicol. 2015;55:613-31. doi: 10.1146/annurev-pharmtox-010814-124852. Epub 2014 Oct 8. Review.

40.

Concise review: tissue-specific microvascular endothelial cells derived from human pluripotent stem cells.

Wilson HK, Canfield SG, Shusta EV, Palecek SP.

Stem Cells. 2014 Dec;32(12):3037-45. doi: 10.1002/stem.1797. Review.

41.

Directed evolution of brain-derived neurotrophic factor for improved folding and expression in Saccharomyces cerevisiae.

Burns ML, Malott TM, Metcalf KJ, Hackel BJ, Chan JR, Shusta EV.

Appl Environ Microbiol. 2014 Sep;80(18):5732-42. doi: 10.1128/AEM.01466-14. Epub 2014 Jul 11.

42.

Liquid crystal droplet-based amplification of microvesicles that are shed by mammalian cells.

Tan LN, Wiepz GJ, Miller DS, Shusta EV, Abbott NL.

Analyst. 2014 May 21;139(10):2386-96. doi: 10.1039/c3an02360e.

43.
44.

Identifying blood-brain-barrier selective single-chain antibody fragments.

Jones AR, Stutz CC, Zhou Y, Marks JD, Shusta EV.

Biotechnol J. 2014 May;9(5):664-74. doi: 10.1002/biot.201300550. Epub 2014 Mar 18.

45.

A retinoic acid-enhanced, multicellular human blood-brain barrier model derived from stem cell sources.

Lippmann ES, Al-Ahmad A, Azarin SM, Palecek SP, Shusta EV.

Sci Rep. 2014 Feb 24;4:4160. doi: 10.1038/srep04160.

46.

αB-crystallin: a novel regulator of breast cancer metastasis to the brain.

Malin D, Strekalova E, Petrovic V, Deal AM, Al Ahmad A, Adamo B, Miller CR, Ugolkov A, Livasy C, Fritchie K, Hamilton E, Blackwell K, Geradts J, Ewend M, Carey L, Shusta EV, Anders CK, Cryns VL.

Clin Cancer Res. 2014 Jan 1;20(1):56-67. doi: 10.1158/1078-0432.CCR-13-1255. Epub 2013 Oct 16.

47.

Facile chemical functionalization of proteins through intein-linked yeast display.

Marshall CJ, Agarwal N, Kalia J, Grosskopf VA, McGrath NA, Abbott NL, Raines RT, Shusta EV.

Bioconjug Chem. 2013 Sep 18;24(9):1634-44. doi: 10.1021/bc4002618. Epub 2013 Aug 22.

48.

Combinatorial approaches for the identification of brain drug delivery targets.

Stutz CC, Zhang X, Shusta EV.

Curr Pharm Des. 2014;20(10):1564-76. Review.

49.

Modeling the blood-brain barrier using stem cell sources.

Lippmann ES, Al-Ahmad A, Palecek SP, Shusta EV.

Fluids Barriers CNS. 2013 Jan 10;10(1):2. doi: 10.1186/2045-8118-10-2.

50.

Antibody affinity maturation using yeast display with detergent-solubilized membrane proteins as antigen sources.

Tillotson BJ, de Larrinoa IF, Skinner CA, Klavas DM, Shusta EV.

Protein Eng Des Sel. 2013 Feb;26(2):101-12. doi: 10.1093/protein/gzs077. Epub 2012 Oct 28.

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