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Items: 19

1.

Various expression-augmenting DNA elements benefit from STAR-Select, a novel high stringency selection system for protein expression.

Otte AP, Kwaks TH, van Blokland RJ, Sewalt RG, Verhees J, Klaren VN, Siersma TK, Korse HW, Teunissen NC, Botschuijver S, van Mer C, Man SY.

Biotechnol Prog. 2007 Jul-Aug;23(4):801-7. Epub 2007 Jun 22.

PMID:
17585780
2.

A novel, high stringency selection system allows screening of few clones for high protein expression.

van Blokland HJ, Kwaks TH, Sewalt RG, Verhees JA, Klaren VN, Siersma TK, Korse JW, Teunissen NC, Botschuijver S, van Mer C, Man SY, Otte AP.

J Biotechnol. 2007 Feb 1;128(2):237-45. Epub 2006 Oct 10.

PMID:
17092592
3.

Expression of the p16(INK4a) gene product, methylation of the p16(INK4a) promoter region and expression of the polycomb-group gene BMI-1 in squamous cell lung carcinoma and premalignant endobronchial lesions.

Breuer RH, Snijders PJ, Sutedja GT, Sewalt RG, Otte AP, Postmus PE, Meijer CJ, Raaphorst FM, Smit EF.

Lung Cancer. 2005 Jun;48(3):299-306.

PMID:
15892997
4.

Increased expression of the EZH2 polycomb group gene in BMI-1-positive neoplastic cells during bronchial carcinogenesis.

Breuer RH, Snijders PJ, Smit EF, Sutedja TG, Sewalt RG, Otte AP, van Kemenade FJ, Postmus PE, Meijer CJ, Raaphorst FM.

Neoplasia. 2004 Nov-Dec;6(6):736-43.

5.

Targeting of a histone acetyltransferase domain to a promoter enhances protein expression levels in mammalian cells.

Kwaks TH, Sewalt RG, van Blokland R, Siersma TJ, Kasiem M, Kelder A, Otte AP.

J Biotechnol. 2005 Jan 12;115(1):35-46.

PMID:
15607223
6.

Distinct expression patterns of polycomb oncoproteins and their binding partners during the germinal center reaction.

van Galen JC, Dukers DF, Giroth C, Sewalt RG, Otte AP, Meijer CJ, Raaphorst FM.

Eur J Immunol. 2004 Jul;34(7):1870-81.

7.

Unique polycomb gene expression pattern in Hodgkin's lymphoma and Hodgkin's lymphoma-derived cell lines.

Dukers DF, van Galen JC, Giroth C, Jansen P, Sewalt RG, Otte AP, Kluin-Nelemans HC, Meijer CJ, Raaphorst FM.

Am J Pathol. 2004 Mar;164(3):873-81.

8.
9.

Site-specific expression of polycomb-group genes encoding the HPC-HPH/PRC1 complex in clinically defined primary nodal and cutaneous large B-cell lymphomas.

Raaphorst FM, Vermeer M, Fieret E, Blokzijl T, Dukers D, Sewalt RG, Otte AP, Willemze R, Meijer CJ.

Am J Pathol. 2004 Feb;164(2):533-42.

10.

EZH2 is a marker of aggressive breast cancer and promotes neoplastic transformation of breast epithelial cells.

Kleer CG, Cao Q, Varambally S, Shen R, Ota I, Tomlins SA, Ghosh D, Sewalt RG, Otte AP, Hayes DF, Sabel MS, Livant D, Weiss SJ, Rubin MA, Chinnaiyan AM.

Proc Natl Acad Sci U S A. 2003 Sep 30;100(20):11606-11. Epub 2003 Sep 19.

11.

Identification of anti-repressor elements that confer high and stable protein production in mammalian cells.

Kwaks TH, Barnett P, Hemrika W, Siersma T, Sewalt RG, Satijn DP, Brons JF, van Blokland R, Kwakman P, Kruckeberg AL, Kelder A, Otte AP.

Nat Biotechnol. 2003 May;21(5):553-8. Epub 2003 Apr 7. Erratum in: Nat Biotechnol. 2003 Jul;21(7):822.

PMID:
12679786
12.

The polycomb group protein EZH2 is involved in progression of prostate cancer.

Varambally S, Dhanasekaran SM, Zhou M, Barrette TR, Kumar-Sinha C, Sanda MG, Ghosh D, Pienta KJ, Sewalt RG, Otte AP, Rubin MA, Chinnaiyan AM.

Nature. 2002 Oct 10;419(6907):624-9.

13.

A panel of monoclonal antibodies against human polycomb group proteins.

Hamer KM, Sewalt RG, den Blaauwen JL, Hendrix T, Satijn DP, Otte AP.

Hybrid Hybridomics. 2002 Aug;21(4):245-52.

PMID:
12193277
14.

Selective interactions between vertebrate polycomb homologs and the SUV39H1 histone lysine methyltransferase suggest that histone H3-K9 methylation contributes to chromosomal targeting of Polycomb group proteins.

Sewalt RG, Lachner M, Vargas M, Hamer KM, den Blaauwen JL, Hendrix T, Melcher M, Schweizer D, Jenuwein T, Otte AP.

Mol Cell Biol. 2002 Aug;22(15):5539-53.

15.

Transcriptional repression mediated by polycomb group proteins and other chromatin-associated repressors is selectively blocked by insulators.

van der Vlag J, den Blaauwen JL, Sewalt RG, van Driel R, Otte AP.

J Biol Chem. 2000 Jan 7;275(1):697-704.

16.

A bipartite sequence element associated with matrix/scaffold attachment regions.

van Drunen CM, Sewalt RG, Oosterling RW, Weisbeek PJ, Smeekens SC, van Driel R.

Nucleic Acids Res. 1999 Jul 15;27(14):2924-30.

17.

C-Terminal binding protein is a transcriptional repressor that interacts with a specific class of vertebrate Polycomb proteins.

Sewalt RG, Gunster MJ, van der Vlag J, Satijn DP, Otte AP.

Mol Cell Biol. 1999 Jan;19(1):777-87.

18.

Characterization of interactions between the mammalian polycomb-group proteins Enx1/EZH2 and EED suggests the existence of different mammalian polycomb-group protein complexes.

Sewalt RG, van der Vlag J, Gunster MJ, Hamer KM, den Blaauwen JL, Satijn DP, Hendrix T, van Driel R, Otte AP.

Mol Cell Biol. 1998 Jun;18(6):3586-95.

19.

Interference with the expression of a novel human polycomb protein, hPc2, results in cellular transformation and apoptosis.

Satijn DP, Olson DJ, van der Vlag J, Hamer KM, Lambrechts C, Masselink H, Gunster MJ, Sewalt RG, van Driel R, Otte AP.

Mol Cell Biol. 1997 Oct;17(10):6076-86.

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