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Items: 13


Escherichia coli "Marionette" strains with 12 highly optimized small-molecule sensors.

Meyer AJ, Segall-Shapiro TH, Glassey E, Zhang J, Voigt CA.

Nat Chem Biol. 2019 Feb;15(2):196-204. doi: 10.1038/s41589-018-0168-3. Epub 2018 Nov 26.


Engineered promoters enable constant gene expression at any copy number in bacteria.

Segall-Shapiro TH, Sontag ED, Voigt CA.

Nat Biotechnol. 2018 Apr;36(4):352-358. doi: 10.1038/nbt.4111. Epub 2018 Mar 19.


The Structure of a Thermophilic Kinase Shapes Fitness upon Random Circular Permutation.

Jones AM, Mehta MM, Thomas EE, Atkinson JT, Segall-Shapiro TH, Liu S, Silberg JJ.

ACS Synth Biol. 2016 May 20;5(5):415-25. doi: 10.1021/acssynbio.5b00305. Epub 2016 Mar 25.


TREC-IN: gene knock-in genetic tool for genomes cloned in yeast.

Chandran S, Noskov VN, Segall-Shapiro TH, Ma L, Whiteis C, Lartigue C, Jores J, Vashee S, Chuang RY.

BMC Genomics. 2014 Dec 24;15:1180. doi: 10.1186/1471-2164-15-1180.


A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase.

Segall-Shapiro TH, Meyer AJ, Ellington AD, Sontag ED, Voigt CA.

Mol Syst Biol. 2014 Jul 30;10:742. doi: 10.15252/msb.20145299.


Advances in genetic circuit design: novel biochemistries, deep part mining, and precision gene expression.

Nielsen AA, Segall-Shapiro TH, Voigt CA.

Curr Opin Chem Biol. 2013 Dec;17(6):878-92. doi: 10.1016/j.cbpa.2013.10.003. Epub 2013 Nov 19. Review.


Design of orthogonal genetic switches based on a crosstalk map of σs, anti-σs, and promoters.

Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA.

Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.


A genetically encoded and gate for cell-targeted metabolic labeling of proteins.

Mahdavi A, Segall-Shapiro TH, Kou S, Jindal GA, Hoff KG, Liu S, Chitsaz M, Ismagilov RF, Silberg JJ, Tirrell DA.

J Am Chem Soc. 2013 Feb 27;135(8):2979-82. doi: 10.1021/ja400448f. Epub 2013 Feb 18.


An adaptor from translational to transcriptional control enables predictable assembly of complex regulation.

Liu CC, Qi L, Lucks JB, Segall-Shapiro TH, Wang D, Mutalik VK, Arkin AP.

Nat Methods. 2012 Nov;9(11):1088-94. doi: 10.1038/nmeth.2184. Epub 2012 Sep 30.


Modular control of multiple pathways using engineered orthogonal T7 polymerases.

Temme K, Hill R, Segall-Shapiro TH, Moser F, Voigt CA.

Nucleic Acids Res. 2012 Sep 1;40(17):8773-81. Epub 2012 Jun 28.


Mesophilic and hyperthermophilic adenylate kinases differ in their tolerance to random fragmentation.

Segall-Shapiro TH, Nguyen PQ, Dos Santos ED, Subedi S, Judd J, Suh J, Silberg JJ.

J Mol Biol. 2011 Feb 11;406(1):135-48. doi: 10.1016/j.jmb.2010.11.057. Epub 2010 Dec 8.


Creation of a bacterial cell controlled by a chemically synthesized genome.

Gibson DG, Glass JI, Lartigue C, Noskov VN, Chuang RY, Algire MA, Benders GA, Montague MG, Ma L, Moodie MM, Merryman C, Vashee S, Krishnakumar R, Assad-Garcia N, Andrews-Pfannkoch C, Denisova EA, Young L, Qi ZQ, Segall-Shapiro TH, Calvey CH, Parmar PP, Hutchison CA 3rd, Smith HO, Venter JC.

Science. 2010 Jul 2;329(5987):52-6. doi: 10.1126/science.1190719. Epub 2010 May 20.


Tandem repeat coupled with endonuclease cleavage (TREC): a seamless modification tool for genome engineering in yeast.

Noskov VN, Segall-Shapiro TH, Chuang RY.

Nucleic Acids Res. 2010 May;38(8):2570-6. doi: 10.1093/nar/gkq099. Epub 2010 Mar 12.

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