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Biotinylation of Membrane Proteins for Binder Selections.

Kuhn BT, Zimmermann I, Egloff P, Hürlimann LM, Hutter CAJ, Miscenic C, Dawson RJP, Seeger MA, Geertsma ER.

Methods Mol Biol. 2020;2127:151-165. doi: 10.1007/978-1-0716-0373-4_11.


The structure of the Legionella response regulator LqsR reveals amino acids critical for phosphorylation and dimerization.

Hochstrasser R, Hutter CAJ, Arnold FM, Bärlocher K, Seeger MA, Hilbi H.

Mol Microbiol. 2020 Jan 29. doi: 10.1111/mmi.14477. [Epub ahead of print]


Spin-labeled nanobodies as protein conformational reporters for electron paramagnetic resonance in cellular membranes.

Galazzo L, Meier G, Timachi MH, Hutter CAJ, Seeger MA, Bordignon E.

Proc Natl Acad Sci U S A. 2020 Feb 4;117(5):2441-2448. doi: 10.1073/pnas.1913737117. Epub 2020 Jan 21.


Mycobacterium marinum produces distinct mycobactin and carboxymycobactin siderophores to promote growth in broth and phagocytes.

Knobloch P, Koliwer-Brandl H, Arnold FM, Hanna N, Gonda I, Adenau S, Personnic N, Barisch C, Seeger MA, Soldati T, Hilbi H.

Cell Microbiol. 2020 Jan 16:e13163. doi: 10.1111/cmi.13163. [Epub ahead of print]


The extracellular gate shapes the energy profile of an ABC exporter.

Hutter CAJ, Timachi MH, Hürlimann LM, Zimmermann I, Egloff P, Göddeke H, Kucher S, Štefanić S, Karttunen M, Schäfer LV, Bordignon E, Seeger MA.

Nat Commun. 2019 May 21;10(1):2260. doi: 10.1038/s41467-019-09892-6.


Identification of conformation-selective nanobodies against the membrane protein insertase BamA by an integrated structural biology approach.

Kaur H, Hartmann JB, Jakob RP, Zahn M, Zimmermann I, Maier T, Seeger MA, Hiller S.

J Biomol NMR. 2019 Jul;73(6-7):375-384. doi: 10.1007/s10858-019-00250-8. Epub 2019 May 9.


Engineered peptide barcodes for in-depth analyses of binding protein libraries.

Egloff P, Zimmermann I, Arnold FM, Hutter CAJ, Morger D, Opitz L, Poveda L, Keserue HA, Panse C, Roschitzki B, Seeger MA.

Nat Methods. 2019 May;16(5):421-428. doi: 10.1038/s41592-019-0389-8. Epub 2019 Apr 22.


Structure and function of Spc42 coiled-coils in yeast centrosome assembly and duplication.

Drennan AC, Krishna S, Seeger MA, Andreas MP, Gardner JM, Sether EKR, Jaspersen SL, Rayment I.

Mol Biol Cell. 2019 Jun 1;30(12):1505-1522. doi: 10.1091/mbc.E19-03-0167. Epub 2019 Apr 10.


Structural basis for pH-dependent retrieval of ER proteins from the Golgi by the KDEL receptor.

Bräuer P, Parker JL, Gerondopoulos A, Zimmermann I, Seeger MA, Barr FA, Newstead S.

Science. 2019 Mar 8;363(6431):1103-1107. doi: 10.1126/science.aaw2859.


Increased drug permeability of a stiffened mycobacterial outer membrane in cells lacking MFS transporter Rv1410 and lipoprotein LprG.

Hohl M, Remm S, Eskandarian HA, Dal Molin M, Arnold FM, Hürlimann LM, Krügel A, Fantner GE, Sander P, Seeger MA.

Mol Microbiol. 2019 May;111(5):1263-1282. doi: 10.1111/mmi.14220. Epub 2019 Mar 18.


A Library-Based Screening Strategy for the Identification of DARPins as Ligands for Receptor-Targeted AAV and Lentiviral Vectors.

Hartmann J, Münch RC, Freiling RT, Schneider IC, Dreier B, Samukange W, Koch J, Seeger MA, Plückthun A, Buchholz CJ.

Mol Ther Methods Clin Dev. 2018 Jul 6;10:128-143. doi: 10.1016/j.omtm.2018.07.001. eCollection 2018 Sep 21.


A uniform cloning platform for mycobacterial genetics and protein production.

Arnold FM, Hohl M, Remm S, Koliwer-Brandl H, Adenau S, Chusri S, Sander P, Hilbi H, Seeger MA.

Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.1038/s41598-018-27687-5.


Synthetic single domain antibodies for the conformational trapping of membrane proteins.

Zimmermann I, Egloff P, Hutter CA, Arnold FM, Stohler P, Bocquet N, Hug MN, Huber S, Siegrist M, Hetemann L, Gera J, Gmür S, Spies P, Gygax D, Geertsma ER, Dawson RJ, Seeger MA.

Elife. 2018 May 24;7. pii: e34317. doi: 10.7554/eLife.34317.


Atomistic Mechanism of Large-Scale Conformational Transition in a Heterodimeric ABC Exporter.

Göddeke H, Timachi MH, Hutter CAJ, Galazzo L, Seeger MA, Karttunen M, Bordignon E, Schäfer LV.

J Am Chem Soc. 2018 Apr 4;140(13):4543-4551. doi: 10.1021/jacs.7b12944. Epub 2018 Mar 26.


Structural insights into Legionella RidL-Vps29 retromer subunit interaction reveal displacement of the regulator TBC1D5.

Bärlocher K, Hutter CAJ, Swart AL, Steiner B, Welin A, Hohl M, Letourneur F, Seeger MA, Hilbi H.

Nat Commun. 2017 Nov 16;8(1):1543. doi: 10.1038/s41467-017-01512-5.


Membrane transporter research in times of countless structures.

Seeger MA.

Biochim Biophys Acta Biomembr. 2018 Apr;1860(4):804-808. doi: 10.1016/j.bbamem.2017.08.009. Epub 2017 Aug 31. Review.


Non-invasive in vivo imaging of tumour-associated cathepsin B by a highly selective inhibitory DARPin.

Kramer L, Renko M, Završnik J, Turk D, Seeger MA, Vasiljeva O, Grütter MG, Turk V, Turk B.

Theranostics. 2017 Jul 8;7(11):2806-2821. doi: 10.7150/thno.19081. eCollection 2017.


Topically Delivered Tumor Necrosis Factor-α-Targeted Gene Regulation for Psoriasis.

Lewandowski KT, Thiede R, Guido N, Daniel WL, Kang R, Guerrero-Zayas MI, Seeger MA, Wang XQ, Giljohann DA, Paller AS.

J Invest Dermatol. 2017 Sep;137(9):2027-2030. doi: 10.1016/j.jid.2017.04.027. Epub 2017 May 11. No abstract available.


Split tasks of asymmetric nucleotide-binding sites in the heterodimeric ABC exporter EfrCD.

Hürlimann LM, Hohl M, Seeger MA.

FEBS J. 2017 Jun;284(11):1672-1687. doi: 10.1111/febs.14065. Epub 2017 Apr 18.


Exploring conformational equilibria of a heterodimeric ABC transporter.

Timachi MH, Hutter CA, Hohl M, Assafa T, Böhm S, Mittal A, Seeger MA, Bordignon E.

Elife. 2017 Jan 4;6. pii: e20236. doi: 10.7554/eLife.20236.


The Heterodimeric ABC Transporter EfrCD Mediates Multidrug Efflux in Enterococcus faecalis.

Hürlimann LM, Corradi V, Hohl M, Bloemberg GV, Tieleman DP, Seeger MA.

Antimicrob Agents Chemother. 2016 Aug 22;60(9):5400-11. doi: 10.1128/AAC.00661-16. Print 2016 Sep.


A Transporter Motor Taken Apart: Flexibility in the Nucleotide Binding Domains of a Heterodimeric ABC Exporter.

Bukowska MA, Hohl M, Geertsma ER, Hürlimann LM, Grütter MG, Seeger MA.

Biochemistry. 2015 May 19;54(19):3086-99. doi: 10.1021/acs.biochem.5b00188. Epub 2015 May 7.


The Roles of Growth Factors in Keratinocyte Migration.

Seeger MA, Paller AS.

Adv Wound Care (New Rochelle). 2015 Apr 1;4(4):213-224. Review.


siRNA-based spherical nucleic acids reverse impaired wound healing in diabetic mice by ganglioside GM3 synthase knockdown.

Randeria PS, Seeger MA, Wang XQ, Wilson H, Shipp D, Mirkin CA, Paller AS.

Proc Natl Acad Sci U S A. 2015 May 5;112(18):5573-8. doi: 10.1073/pnas.1505951112. Epub 2015 Apr 20.


Bicistronic mRNAs to enhance membrane protein overexpression.

Marino J, Hohl M, Seeger MA, Zerbe O, Geertsma ER.

J Mol Biol. 2015 Feb 27;427(4):943-954. doi: 10.1016/j.jmb.2014.11.002. Epub 2014 Nov 9.


Coupling of remote alternating-access transport mechanisms for protons and substrates in the multidrug efflux pump AcrB.

Eicher T, Seeger MA, Anselmi C, Zhou W, Brandstätter L, Verrey F, Diederichs K, Faraldo-Gómez JD, Pos KM.

Elife. 2014 Sep 19;3. doi: 10.7554/eLife.03145.


Structural basis for allosteric cross-talk between the asymmetric nucleotide binding sites of a heterodimeric ABC exporter.

Hohl M, Hürlimann LM, Böhm S, Schöppe J, Grütter MG, Bordignon E, Seeger MA.

Proc Natl Acad Sci U S A. 2014 Jul 29;111(30):11025-30. doi: 10.1073/pnas.1400485111. Epub 2014 Jul 16.


Intrinsic Disorder in the Kinesin Superfamily.

Seeger MA, Rice SE.

Biophys Rev. 2013 Sep 1;5(3). doi: 10.1007/s12551-012-0096-5.


The role of abnormalities in the distal pathway of cholesterol synthesis in the Congenital Hemidysplasia with Ichthyosiform erythroderma and Limb Defects (CHILD) syndrome.

Seeger MA, Paller AS.

Biochim Biophys Acta. 2014 Mar;1841(3):345-52. doi: 10.1016/j.bbalip.2013.09.006. Epub 2013 Sep 20. Review.


Design, construction, and characterization of a second-generation DARP in library with reduced hydrophobicity.

Seeger MA, Zbinden R, Flütsch A, Gutte PG, Engeler S, Roschitzki-Voser H, Grütter MG.

Protein Sci. 2013 Sep;22(9):1239-57. doi: 10.1002/pro.2312. Epub 2013 Aug 6.


A genome-wide RNAi screen reveals a Trio-regulated Rho GTPase circuitry transducing mitogenic signals initiated by G protein-coupled receptors.

Vaqué JP, Dorsam RT, Feng X, Iglesias-Bartolome R, Forsthoefel DJ, Chen Q, Debant A, Seeger MA, Ksander BR, Teramoto H, Gutkind JS.

Mol Cell. 2013 Jan 10;49(1):94-108. doi: 10.1016/j.molcel.2012.10.018. Epub 2012 Nov 21.


X-Ray Structure of a Heterodimeric ABC Transporter Crystallized in its Inward-Facing Conformation.

Hohl M, Grütter MG, Seeger MA.

Chimia (Aarau). 2012 Oct 31;66(10):802. doi: 10.2533/chimia.2012.802. No abstract available.


Tuning the drug efflux activity of an ABC transporter in vivo by in vitro selected DARPin binders.

Seeger MA, Mittal A, Velamakanni S, Hohl M, Schauer S, Salaa I, Grütter MG, van Veen HW.

PLoS One. 2012;7(6):e37845. doi: 10.1371/journal.pone.0037845. Epub 2012 Jun 4.


Kinesin tail domains are intrinsically disordered.

Seeger MA, Zhang Y, Rice SE.

Proteins. 2012 Oct;80(10):2437-46. doi: 10.1002/prot.24128. Epub 2012 Jul 7.


Asymmetry in the homodimeric ABC transporter MsbA recognized by a DARPin.

Mittal A, Böhm S, Grütter MG, Bordignon E, Seeger MA.

J Biol Chem. 2012 Jun 8;287(24):20395-406. doi: 10.1074/jbc.M112.359794. Epub 2012 Apr 20.


Transport of drugs by the multidrug transporter AcrB involves an access and a deep binding pocket that are separated by a switch-loop.

Eicher T, Cha HJ, Seeger MA, Brandstätter L, El-Delik J, Bohnert JA, Kern WV, Verrey F, Grütter MG, Diederichs K, Pos KM.

Proc Natl Acad Sci U S A. 2012 Apr 10;109(15):5687-92. doi: 10.1073/pnas.1114944109. Epub 2012 Mar 26.


Crystal structure of a heterodimeric ABC transporter in its inward-facing conformation.

Hohl M, Briand C, Grütter MG, Seeger MA.

Nat Struct Mol Biol. 2012 Mar 25;19(4):395-402. doi: 10.1038/nsmb.2267.


Analysis of AcrB and AcrB/DARPin ligand complexes by LILBID MS.

Brandstätter L, Sokolova L, Eicher T, Seeger MA, Briand C, Cha HJ, Cernescu M, Bohnert J, Kern WV, Brutschy B, Pos KM.

Biochim Biophys Acta. 2011 Sep;1808(9):2189-96. doi: 10.1016/j.bbamem.2011.05.009. Epub 2011 May 15.


Designed ankyrin repeat protein binders for the crystallization of AcrB: plasticity of the dominant interface.

Monroe N, Sennhauser G, Seeger MA, Briand C, Grütter MG.

J Struct Biol. 2011 May;174(2):269-81. doi: 10.1016/j.jsb.2011.01.014. Epub 2011 Feb 4.


In the absence of frazzled over-expression of Abelson tyrosine kinase disrupts commissure formation and causes axons to leave the embryonic CNS.

Dorsten JN, Varughese BE, Karmo S, Seeger MA, VanBerkum MF.

PLoS One. 2010 Mar 23;5(3):e9822. doi: 10.1371/journal.pone.0009822.


Microtubule-associated protein-like binding of the kinesin-1 tail to microtubules.

Seeger MA, Rice SE.

J Biol Chem. 2010 Mar 12;285(11):8155-62. doi: 10.1074/jbc.M109.068247. Epub 2010 Jan 12.


A multidrug ABC transporter with a taste for salt.

Velamakanni S, Lau CH, Gutmann DA, Venter H, Barrera NP, Seeger MA, Woebking B, Matak-Vinkovic D, Balakrishnan L, Yao Y, U EC, Shilling RA, Robinson CV, Thorn P, van Veen HW.

PLoS One. 2009 Jul 10;4(7):e6137. doi: 10.1371/journal.pone.0006137.


Mass spectrometry of membrane transporters reveals subunit stoichiometry and interactions.

Barrera NP, Isaacson SC, Zhou M, Bavro VN, Welch A, Schaedler TA, Seeger MA, Miguel RN, Korkhov VM, van Veen HW, Venter H, Walmsley AR, Tate CG, Robinson CV.

Nat Methods. 2009 Aug;6(8):585-7. doi: 10.1038/nmeth.1347. Epub 2009 Jul 5.


Crucial role of Asp408 in the proton translocation pathway of multidrug transporter AcrB: evidence from site-directed mutagenesis and carbodiimide labeling.

Seeger MA, von Ballmoos C, Verrey F, Pos KM.

Biochemistry. 2009 Jun 30;48(25):5801-12. doi: 10.1021/bi900446j.


Molecular basis of multidrug transport by ABC transporters.

Seeger MA, van Veen HW.

Biochim Biophys Acta. 2009 May;1794(5):725-37. doi: 10.1016/j.bbapap.2008.12.004. Epub 2008 Dec 24. Review.


Site-directed mutagenesis reveals putative substrate binding residues in the Escherichia coli RND efflux pump AcrB.

Bohnert JA, Schuster S, Seeger MA, Fähnrich E, Pos KM, Kern WV.

J Bacteriol. 2008 Dec;190(24):8225-9. doi: 10.1128/JB.00912-08. Epub 2008 Oct 10.


Functional role of transmembrane helix 6 in drug binding and transport by the ABC transporter MsbA.

Woebking B, Velamakanni S, Federici L, Seeger MA, Murakami S, van Veen HW.

Biochemistry. 2008 Oct 14;47(41):10904-14. doi: 10.1021/bi800778d. Epub 2008 Sep 20.


The AcrB efflux pump: conformational cycling and peristalsis lead to multidrug resistance.

Seeger MA, Diederichs K, Eicher T, Brandstätter L, Schiefner A, Verrey F, Pos KM.

Curr Drug Targets. 2008 Sep;9(9):729-49. Review.


Engineered disulfide bonds support the functional rotation mechanism of multidrug efflux pump AcrB.

Seeger MA, von Ballmoos C, Eicher T, Brandstätter L, Verrey F, Diederichs K, Pos KM.

Nat Struct Mol Biol. 2008 Feb;15(2):199-205. doi: 10.1038/nsmb.1379. Epub 2008 Jan 27.


Structural asymmetry of AcrB trimer suggests a peristaltic pump mechanism.

Seeger MA, Schiefner A, Eicher T, Verrey F, Diederichs K, Pos KM.

Science. 2006 Sep 1;313(5791):1295-8.

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