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Items: 1 to 50 of 110


High-resolution restoration of 3D structures from widefield images with extreme low signal-to-noise-ratio.

Arigovindan M, Fung JC, Elnatan D, Mennella V, Chan YH, Pollard M, Branlund E, Sedat JW, Agard DA.

Proc Natl Acad Sci U S A. 2013 Oct 22;110(43):17344-9. doi: 10.1073/pnas.1315675110. Epub 2013 Oct 8.


Interferometer-based structured-illumination microscopy utilizing complementary phase relationship through constructive and destructive image detection by two cameras.

Shao L, Winoto L, Agard DA, Gustafsson MG, Sedat JW.

J Microsc. 2012 Jun;246(3):229-36. doi: 10.1111/j.1365-2818.2012.03604.x. Epub 2012 Apr 4.


Effect of depth dependent spherical aberrations in 3D structured illumination microscopy.

Arigovindan M, Sedat JW, Agard DA.

Opt Express. 2012 Mar 12;20(6):6527-41. doi: 10.1364/OE.20.006527.


OMX: a new platform for multimodal, multichannel wide-field imaging.

Dobbie IM, King E, Parton RM, Carlton PM, Sedat JW, Swedlow JR, Davis I.

Cold Spring Harb Protoc. 2011 Aug 1;2011(8):899-909. doi: 10.1101/pdb.top121. No abstract available.


A distributed multi-GPU system for high speed electron microscopic tomographic reconstruction.

Zheng SQ, Branlund E, Kesthelyi B, Braunfeld MB, Cheng Y, Sedat JW, Agard DA.

Ultramicroscopy. 2011 Jul;111(8):1137-43. doi: 10.1016/j.ultramic.2011.03.015. Epub 2011 Apr 1.


Evidence of activity-specific, radial organization of mitotic chromosomes in Drosophila.

Strukov YG, Sural TH, Kuroda MI, Sedat JW.

PLoS Biol. 2011 Jan 11;9(1):e1000574. doi: 10.1371/journal.pbio.1000574.


Automated data collection for electron microscopic tomography.

Zheng SQ, Sedat JW, Agard DA.

Methods Enzymol. 2010;481:283-315. doi: 10.1016/S0076-6879(10)81012-2.


Condensed mitotic chromosome structure at nanometer resolution using PALM and EGFP- histones.

Matsuda A, Shao L, Boulanger J, Kervrann C, Carlton PM, Kner P, Agard D, Sedat JW.

PLoS One. 2010 Sep 15;5(9):e12768. doi: 10.1371/journal.pone.0012768.


Fast live simultaneous multiwavelength four-dimensional optical microscopy.

Carlton PM, Boulanger J, Kervrann C, Sibarita JB, Salamero J, Gordon-Messer S, Bressan D, Haber JE, Haase S, Shao L, Winoto L, Matsuda A, Kner P, Uzawa S, Gustafsson M, Kam Z, Agard DA, Sedat JW.

Proc Natl Acad Sci U S A. 2010 Sep 14;107(37):16016-22. doi: 10.1073/pnas.1004037107. Epub 2010 Aug 12.


A Parallel Product-Convolution approach for representing the depth varying Point Spread Functions in 3D widefield microscopy based on principal component analysis.

Arigovindan M, Shaevitz J, McGowan J, Sedat JW, Agard DA.

Opt Express. 2010 Mar 29;18(7):6461-76. doi: 10.1364/OE.18.006461.


Closed loop adaptive optics for microscopy without a wavefront sensor.

Kner P, Winoto L, Agard DA, Sedat JW.

Proc SPIE Int Soc Opt Eng. 2010 Feb 24;7570. doi: 10.1117/12.840943.


High-resolution wide-field microscopy with adaptive optics for spherical aberration correction and motionless focusing.

Kner P, Sedat JW, Agard DA, Kam Z.

J Microsc. 2010 Feb;237(2):136-47. doi: 10.1111/j.1365-2818.2009.03315.x.


Dual-axis target mapping and automated sequential acquisition of dual-axis EM tomographic data.

Zheng SQ, Matsuda A, Braunfeld MB, Sedat JW, Agard DA.

J Struct Biol. 2009 Nov;168(2):323-31. doi: 10.1016/j.jsb.2009.06.010. Epub 2009 Jun 21.


Rapid telomere motions in live human cells analyzed by highly time-resolved microscopy.

Wang X, Kam Z, Carlton PM, Xu L, Sedat JW, Blackburn EH.

Epigenetics Chromatin. 2008 Oct 27;1(1):4. doi: 10.1186/1756-8935-1-4.


Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy.

Schermelleh L, Carlton PM, Haase S, Shao L, Winoto L, Kner P, Burke B, Cardoso MC, Agard DA, Gustafsson MG, Leonhardt H, Sedat JW.

Science. 2008 Jun 6;320(5881):1332-6. doi: 10.1126/science.1156947.


N-terminal processing of proteins exported by malaria parasites.

Chang HH, Falick AM, Carlton PM, Sedat JW, DeRisi JL, Marletta MA.

Mol Biochem Parasitol. 2008 Aug;160(2):107-15. doi: 10.1016/j.molbiopara.2008.04.011. Epub 2008 May 2.


A presynaptic giant ankyrin stabilizes the NMJ through regulation of presynaptic microtubules and transsynaptic cell adhesion.

Pielage J, Cheng L, Fetter RD, Carlton PM, Sedat JW, Davis GW.

Neuron. 2008 Apr 24;58(2):195-209. doi: 10.1016/j.neuron.2008.02.017.


Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination.

Gustafsson MG, Shao L, Carlton PM, Wang CJ, Golubovskaya IN, Cande WZ, Agard DA, Sedat JW.

Biophys J. 2008 Jun;94(12):4957-70. doi: 10.1529/biophysj.107.120345. Epub 2008 Mar 7.


I5S: wide-field light microscopy with 100-nm-scale resolution in three dimensions.

Shao L, Isaac B, Uzawa S, Agard DA, Sedat JW, Gustafsson MG.

Biophys J. 2008 Jun;94(12):4971-83. doi: 10.1529/biophysj.107.120352. Epub 2008 Mar 7.


AIDA: an adaptive image deconvolution algorithm with application to multi-frame and three-dimensional data.

Hom EF, Marchis F, Lee TK, Haase S, Agard DA, Sedat JW.

J Opt Soc Am A Opt Image Sci Vis. 2007 Jun;24(6):1580-600.


Modelling the application of adaptive optics to wide-field microscope live imaging.

Kam Z, Kner P, Agard D, Sedat JW.

J Microsc. 2007 Apr;226(Pt 1):33-42.


Wavelet-based characterization of vertebral trabecular bone structure from magnetic resonance images at 3 T compared with micro-computed tomographic measurements.

Krug R, Carballido-Gamio J, Burghardt AJ, Haase S, Sedat JW, Moss WC, Majumdar S.

Magn Reson Imaging. 2007 Apr;25(3):392-8. Epub 2006 Nov 14.


The three-dimensional structure of in vitro reconstituted Xenopus laevis chromosomes by EM tomography.

König P, Braunfeld MB, Sedat JW, Agard DA.

Chromosoma. 2007 Aug;116(4):349-72. Epub 2007 Feb 28.


Automated acquisition of electron microscopic random conical tilt sets.

Zheng SQ, Kollman JM, Braunfeld MB, Sedat JW, Agard DA.

J Struct Biol. 2007 Jan;157(1):148-55. Epub 2006 Nov 3.


UCSF tomography: an integrated software suite for real-time electron microscopic tomographic data collection, alignment, and reconstruction.

Zheng SQ, Keszthelyi B, Branlund E, Lyle JM, Braunfeld MB, Sedat JW, Agard DA.

J Struct Biol. 2007 Jan;157(1):138-47. Epub 2006 Jun 23.


The Mcp element mediates stable long-range chromosome-chromosome interactions in Drosophila.

Vazquez J, Müller M, Pirrotta V, Sedat JW.

Mol Biol Cell. 2006 May;17(5):2158-65. Epub 2006 Feb 22.


A novel 3D wavelet-based filter for visualizing features in noisy biological data.

Moss WC, Haase S, Lyle JM, Agard DA, Sedat JW.

J Microsc. 2005 Aug;219(Pt 2):43-9.


Phase-retrieved pupil functions in wide-field fluorescence microscopy.

Hanser BM, Gustafsson MG, Agard DA, Sedat JW.

J Microsc. 2004 Oct;216(Pt 1):32-48.


An improved strategy for automated electron microscopic tomography.

Zheng QS, Braunfeld MB, Sedat JW, Agard DA.

J Struct Biol. 2004 Aug;147(2):91-101.


Phase retrieval for high-numerical-aperture optical systems.

Hanser BM, Gustafsson MG, Agard DA, Sedat JW.

Opt Lett. 2003 May 15;28(10):801-3.


The dynamics of homologous chromosome pairing during male Drosophila meiosis.

Vazquez J, Belmont AS, Sedat JW.

Curr Biol. 2002 Sep 3;12(17):1473-83.


Multiple regimes of constrained chromosome motion are regulated in the interphase Drosophila nucleus.

Vazquez J, Belmont AS, Sedat JW.

Curr Biol. 2001 Aug 21;11(16):1227-39.


Early transcription and silencing of cytokine genes underlie polarization of T helper cell subsets.

Grogan JL, Mohrs M, Harmon B, Lacy DA, Sedat JW, Locksley RM.

Immunity. 2001 Mar;14(3):205-15.


Computational adaptive optics for live three-dimensional biological imaging.

Kam Z, Hanser B, Gustafsson MG, Agard DA, Sedat JW.

Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):3790-5.


Evidence for the coincident initiation of homolog pairing and synapsis during the telomere-clustering (bouquet) stage of meiotic prophase.

Bass HW, Riera-Lizarazu O, Ananiev EV, Bordoli SJ, Rines HW, Phillips RL, Sedat JW, Agard DA, Cande WZ.

J Cell Sci. 2000 Mar;113 ( Pt 6):1033-42.


Polarization of chemoattractant receptor signaling during neutrophil chemotaxis.

Servant G, Weiner OD, Herzmark P, Balla T, Sedat JW, Bourne HR.

Science. 2000 Feb 11;287(5455):1037-40.


Enteropathogenic E. coli acts through WASP and Arp2/3 complex to form actin pedestals.

Kalman D, Weiner OD, Goosney DL, Sedat JW, Finlay BB, Abo A, Bishop JM.

Nat Cell Biol. 1999 Oct;1(6):389-91. No abstract available.


Spatial control of actin polymerization during neutrophil chemotaxis.

Weiner OD, Servant G, Welch MD, Mitchison TJ, Sedat JW, Bourne HR.

Nat Cell Biol. 1999 Jun;1(2):75-81.


I5M: 3D widefield light microscopy with better than 100 nm axial resolution.

Gustafsson MG, Agard DA, Sedat JW.

J Microsc. 1999 Jul;195(Pt 1):10-6.


Nuclear architecture.

Marshall WF, Sedat JW.

Results Probl Cell Differ. 1999;25:283-301. Review. No abstract available.


Dynamics of a chemoattractant receptor in living neutrophils during chemotaxis.

Servant G, Weiner OD, Neptune ER, Sedat JW, Bourne HR.

Mol Biol Cell. 1999 Apr;10(4):1163-78.


Time-lapse microscopy reveals unique roles for kinesins during anaphase in budding yeast.

Straight AF, Sedat JW, Murray AW.

J Cell Biol. 1998 Nov 2;143(3):687-94.


Homologous chromosome pairing in Drosophila melanogaster proceeds through multiple independent initiations.

Fung JC, Marshall WF, Dernburg A, Agard DA, Sedat JW.

J Cell Biol. 1998 Apr 6;141(1):5-20.


Interphase chromosomes undergo constrained diffusional motion in living cells.

Marshall WF, Straight A, Marko JF, Swedlow J, Dernburg A, Belmont A, Murray AW, Agard DA, Sedat JW.

Curr Biol. 1997 Dec 1;7(12):930-9.


Mapping three-dimensional chromosome architecture in situ.

Dernburg AF, Sedat JW.

Methods Cell Biol. 1998;53:187-233. Review. No abstract available.


Mitosis in living budding yeast: anaphase A but no metaphase plate.

Straight AF, Marshall WF, Sedat JW, Murray AW.

Science. 1997 Jul 25;277(5325):574-8.


Two proteins that cycle asynchronously between centrosomes and nuclear structures: Drosophila CP60 and CP190.

Oegema K, Marshall WF, Sedat JW, Alberts BM.

J Cell Sci. 1997 Jul;110 ( Pt 14):1573-83.

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