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Items: 12


High-throughput single-cell DNA sequencing of acute myeloid leukemia tumors with droplet microfluidics.

Pellegrino M, Sciambi A, Treusch S, Durruthy-Durruthy R, Gokhale K, Jacob J, Chen TX, Geis JA, Oldham W, Matthews J, Kantarjian H, Futreal PA, Patel K, Jones KW, Takahashi K, Eastburn DJ.

Genome Res. 2018 Sep;28(9):1345-1352. doi: 10.1101/gr.232272.117. Epub 2018 Aug 7.


Lgr6 is a stem cell marker in mouse skin squamous cell carcinoma.

Huang PY, Kandyba E, Jabouille A, Sjolund J, Kumar A, Halliwill K, McCreery M, DelRosario R, Kang HC, Wong CE, Seibler J, Beuger V, Pellegrino M, Sciambi A, Eastburn DJ, Balmain A.

Nat Genet. 2017 Nov;49(11):1624-1632. doi: 10.1038/ng.3957. Epub 2017 Sep 25.


RNA-Seq following PCR-based sorting reveals rare cell transcriptional signatures.

Pellegrino M, Sciambi A, Yates JL, Mast JD, Silver C, Eastburn DJ.

BMC Genomics. 2016 May 17;17:361. doi: 10.1186/s12864-016-2694-2.


Microfluidic droplet enrichment for targeted sequencing.

Eastburn DJ, Huang Y, Pellegrino M, Sciambi A, Ptáček LJ, Abate AR.

Nucleic Acids Res. 2015 Jul 27;43(13):e86. doi: 10.1093/nar/gkv297. Epub 2015 Apr 14.


Accurate microfluidic sorting of droplets at 30 kHz.

Sciambi A, Abate AR.

Lab Chip. 2015 Jan 7;15(1):47-51. doi: 10.1039/c4lc01194e.


Identification and genetic analysis of cancer cells with PCR-activated cell sorting.

Eastburn DJ, Sciambi A, Abate AR.

Nucleic Acids Res. 2014;42(16):e128. doi: 10.1093/nar/gku606. Epub 2014 Jul 16.


Picoinjection of microfluidic drops without metal electrodes.

O'Donovan B, Tran T, Sciambi A, Abate A.

J Vis Exp. 2014 Apr 18;(86). doi: 10.3791/50913.


Generating electric fields in PDMS microfluidic devices with salt water electrodes.

Sciambi A, Abate AR.

Lab Chip. 2014 Aug 7;14(15):2605-9. doi: 10.1039/c4lc00078a. Epub 2014 Mar 27.


Adding reagent to droplets with controlled rupture of encapsulated double emulsions.

Sciambi A, Abate AR.

Biomicrofluidics. 2013 Aug 5;7(4):44112. doi: 10.1063/1.4817793. eCollection 2013.


Ultrahigh-throughput Mammalian single-cell reverse-transcriptase polymerase chain reaction in microfluidic drops.

Eastburn DJ, Sciambi A, Abate AR.

Anal Chem. 2013 Aug 20;85(16):8016-21. doi: 10.1021/ac402057q. Epub 2013 Aug 8.


Picoinjection enables digital detection of RNA with droplet rt-PCR.

Eastburn DJ, Sciambi A, Abate AR.

PLoS One. 2013 Apr 26;8(4):e62961. doi: 10.1371/journal.pone.0062961. Print 2013.


Design of a scanning gate microscope for mesoscopic electron systems in a cryogen-free dilution refrigerator.

Pelliccione M, Sciambi A, Bartel J, Keller AJ, Goldhaber-Gordon D.

Rev Sci Instrum. 2013 Mar;84(3):033703. doi: 10.1063/1.4794767.


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