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Plant Physiol. 2019 Mar 12. pii: pp.01432.2018. doi: 10.1104/pp.18.01432. [Epub ahead of print]

Highly resolved systems biology to dissect the etioplast-to-chloroplast transition in tobacco leaves.

Author information

1
Max Planck Institute of Molecular Plant Physiology CITY: Potsdam-Golm Germany [DE].
2
University of Warsaw CITY: Warszawa Poland [PL].
3
Universidad de Guadalajara CITY: Guadalajara Mexico [MX].
4
Max Planck Institute of Molecular Plant Physiology CITY: Potsdam STATE: Brandenburg POSTAL_CODE: 14476 Germany [DE].
5
MPI of Molecular Plant Physiology, Golm CITY: D-14476 Potsdam-Golm STATE: Brandenburg Germany [DE].
6
Max Planck Institute for Molecular Plant Physiology CITY: Potsdam.Golm Germany [DE].
7
Max Planck Institute for Molecular Plant Physiology CITY: Potsdam Germany [DE].
8
Max-Planck-Institut for Molecular Plant Physiology CITY: Potsdam POSTAL_CODE: 14476 Germany [DE].
9
Max Planck Institute of Molecular Plant Physiology CITY: Potsdam Germany [DE].
10
Max Planck Institute of Molecular Plant Physiology CITY: Potsdam-Golm STATE: Br POSTAL_CODE: 14476 Germany [DE].
11
MPIMP N/A CITY: N/A POSTAL_CODE: N/A Germany [DE].
12
Weizmann Institute of Science CITY: Rehovot POSTAL_CODE: 76100 Israel [IL].
13
Max Planck Institute of Molecular Plant Physiology CITY: D-14476 Potsdam-Golm Germany [DE].
14
Max Planck Institute of Molecular Plant Physiology CITY: D-14476 Potsdam-Golm Germany [DE] rbock@mpimp-golm.mpg.de.

Abstract

Upon exposure to light, plant cells quickly acquire photosynthetic competence by converting pale etioplasts into green chloroplasts. This developmental transition involves the de novo biogenesis of the thylakoid system, and requires reprogramming of metabolism and gene expression. Etioplast-to-chloroplast differentiation involves massive changes in plastid ultrastructure, but how these changes are connected to specific changes in physiology, metabolism and expression of the plastid and nuclear genomes is poorly understood. Here, we describe a new experimental system in the dicotyledonous model plant tobacco (Nicotiana tabacum) that allows us to study the leaf de-etiolation process at the systems level. We have determined the accumulation kinetics of photosynthetic complexes, pigments, lipids and soluble metabolites, and recorded the dynamic changes in plastid ultrastructure and in the nuclear and plastid transcriptomes. Our data describe the greening process at high temporal resolution, resolve distinct genetic and metabolic phases during de-etiolation, and reveal numerous candidate genes that may be involved in light-induced chloroplast development and thylakoid biogenesis.

PMID:
30862726
DOI:
10.1104/pp.18.01432
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