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Items: 1 to 50 of 56

1.

Super-resolution microscopy demystified.

Schermelleh L, Ferrand A, Huser T, Eggeling C, Sauer M, Biehlmaier O, Drummen GPC.

Nat Cell Biol. 2019 Jan;21(1):72-84. doi: 10.1038/s41556-018-0251-8. Epub 2019 Jan 2. Review.

PMID:
30602772
2.

Microscope calibration using laser written fluorescence.

Corbett AD, Shaw M, Yacoot A, Jefferson A, Schermelleh L, Wilson T, Booth M, Salter PS.

Opt Express. 2018 Aug 20;26(17):21887-21899. doi: 10.1364/OE.26.021887.

PMID:
30130891
3.

Accurate and fiducial-marker-free correction for three-dimensional chromatic shift in biological fluorescence microscopy.

Matsuda A, Schermelleh L, Hirano Y, Haraguchi T, Hiraoka Y.

Sci Rep. 2018 May 15;8(1):7583. doi: 10.1038/s41598-018-25922-7.

4.

The SET1 Complex Selects Actively Transcribed Target Genes via Multivalent Interaction with CpG Island Chromatin.

Brown DA, Di Cerbo V, Feldmann A, Ahn J, Ito S, Blackledge NP, Nakayama M, McClellan M, Dimitrova E, Turberfield AH, Long HK, King HW, Kriaucionis S, Schermelleh L, Kutateladze TG, Koseki H, Klose RJ.

Cell Rep. 2017 Sep 5;20(10):2313-2327. doi: 10.1016/j.celrep.2017.08.030.

5.

PCGF3/5-PRC1 initiates Polycomb recruitment in X chromosome inactivation.

Almeida M, Pintacuda G, Masui O, Koseki Y, Gdula M, Cerase A, Brown D, Mould A, Innocent C, Nakayama M, Schermelleh L, Nesterova TB, Koseki H, Brockdorff N.

Science. 2017 Jun 9;356(6342):1081-1084. doi: 10.1126/science.aal2512.

PMID:
28596365
6.

Strategic and practical guidelines for successful structured illumination microscopy.

Demmerle J, Innocent C, North AJ, Ball G, Müller M, Miron E, Matsuda A, Dobbie IM, Markaki Y, Schermelleh L.

Nat Protoc. 2017 May;12(5):988-1010. doi: 10.1038/nprot.2017.019. Epub 2017 Apr 13. Review.

PMID:
28406496
7.

Quantitative 3D structured illumination microscopy of nuclear structures.

Kraus F, Miron E, Demmerle J, Chitiashvili T, Budco A, Alle Q, Matsuda A, Leonhardt H, Schermelleh L, Markaki Y.

Nat Protoc. 2017 May;12(5):1011-1028. doi: 10.1038/nprot.2017.020. Epub 2017 Apr 13. Review.

PMID:
28406495
8.

In Vivo and In Situ Replication Labeling Methods for Super-resolution Structured Illumination Microscopy of Chromosome Territories and Chromatin Domains.

Miron E, Innocent C, Heyde S, Schermelleh L.

Methods Mol Biol. 2016;1431:127-40. doi: 10.1007/978-1-4939-3631-1_10.

PMID:
27283306
9.

4D Visualization of replication foci in mammalian cells corresponding to individual replicons.

Chagin VO, Casas-Delucchi CS, Reinhart M, Schermelleh L, Markaki Y, Maiser A, Bolius JJ, Bensimon A, Fillies M, Domaing P, Rozanov YM, Leonhardt H, Cardoso MC.

Nat Commun. 2016 Apr 7;7:11231. doi: 10.1038/ncomms11231.

10.

Erratum: SIMcheck: a Toolbox for Successful Super-resolution Structured Illumination Microscopy.

Ball G, Demmerle J, Kaufmann R, Davis I, Dobbie IM, Schermelleh L.

Sci Rep. 2016 Feb 5;6:20754. doi: 10.1038/srep20754. No abstract available.

11.

Structural Basis of Vesicle Formation at the Inner Nuclear Membrane.

Hagen C, Dent KC, Zeev-Ben-Mordehai T, Grange M, Bosse JB, Whittle C, Klupp BG, Siebert CA, Vasishtan D, Bäuerlein FJ, Cheleski J, Werner S, Guttmann P, Rehbein S, Henzler K, Demmerle J, Adler B, Koszinowski U, Schermelleh L, Schneider G, Enquist LW, Plitzko JM, Mettenleiter TC, Grünewald K.

Cell. 2015 Dec 17;163(7):1692-701. doi: 10.1016/j.cell.2015.11.029.

12.

SIMcheck: a Toolbox for Successful Super-resolution Structured Illumination Microscopy.

Ball G, Demmerle J, Kaufmann R, Davis I, Dobbie IM, Schermelleh L.

Sci Rep. 2015 Nov 3;5:15915. doi: 10.1038/srep15915. Erratum in: Sci Rep. 2016;6:20754.

13.

A Pooled shRNA Screen Identifies Rbm15, Spen, and Wtap as Factors Required for Xist RNA-Mediated Silencing.

Moindrot B, Cerase A, Coker H, Masui O, Grijzenhout A, Pintacuda G, Schermelleh L, Nesterova TB, Brockdorff N.

Cell Rep. 2015 Jul 28;12(4):562-72. doi: 10.1016/j.celrep.2015.06.053. Epub 2015 Jul 16.

14.

Assessing resolution in super-resolution imaging.

Demmerle J, Wegel E, Schermelleh L, Dobbie IM.

Methods. 2015 Oct 15;88:3-10. doi: 10.1016/j.ymeth.2015.07.001. Epub 2015 Jul 8.

PMID:
26164701
15.

A molecular mechanism of mitotic centrosome assembly in Drosophila.

Conduit PT, Richens JH, Wainman A, Holder J, Vicente CC, Pratt MB, Dix CI, Novak ZA, Dobbie IM, Schermelleh L, Raff JW.

Elife. 2014 Aug 22;3:e03399. doi: 10.7554/eLife.03399.

16.

Three-dimensional super-resolution microscopy of the inactive X chromosome territory reveals a collapse of its active nuclear compartment harboring distinct Xist RNA foci.

Smeets D, Markaki Y, Schmid VJ, Kraus F, Tattermusch A, Cerase A, Sterr M, Fiedler S, Demmerle J, Popken J, Leonhardt H, Brockdorff N, Cremer T, Schermelleh L, Cremer M.

Epigenetics Chromatin. 2014 Apr 28;7:8. doi: 10.1186/1756-8935-7-8. eCollection 2014.

17.

Targeting polycomb to pericentric heterochromatin in embryonic stem cells reveals a role for H2AK119u1 in PRC2 recruitment.

Cooper S, Dienstbier M, Hassan R, Schermelleh L, Sharif J, Blackledge NP, De Marco V, Elderkin S, Koseki H, Klose R, Heger A, Brockdorff N.

Cell Rep. 2014 Jun 12;7(5):1456-1470. doi: 10.1016/j.celrep.2014.04.012. Epub 2014 May 22.

18.

Simultaneous dual-color 3D STED microscopy.

Osseforth C, Moffitt JR, Schermelleh L, Michaelis J.

Opt Express. 2014 Mar 24;22(6):7028-39. doi: 10.1364/OE.22.007028.

PMID:
24664051
19.

Spatial separation of Xist RNA and polycomb proteins revealed by superresolution microscopy.

Cerase A, Smeets D, Tang YA, Gdula M, Kraus F, Spivakov M, Moindrot B, Leleu M, Tattermusch A, Demmerle J, Nesterova TB, Green C, Otte AP, Schermelleh L, Brockdorff N.

Proc Natl Acad Sci U S A. 2014 Feb 11;111(6):2235-40. doi: 10.1073/pnas.1312951111. Epub 2014 Jan 27.

20.

RecA bundles mediate homology pairing between distant sisters during DNA break repair.

Lesterlin C, Ball G, Schermelleh L, Sherratt DJ.

Nature. 2014 Feb 13;506(7487):249-53. doi: 10.1038/nature12868. Epub 2013 Dec 22.

21.

The polyserine domain of the lysyl-5 hydroxylase Jmjd6 mediates subnuclear localization.

Wolf A, Mantri M, Heim A, Müller U, Fichter E, Mackeen MM, Schermelleh L, Dadie G, Leonhardt H, Vénien-Bryan C, Kessler BM, Schofield CJ, Böttger A.

Biochem J. 2013 Aug 1;453(3):357-70. doi: 10.1042/BJ20130529.

PMID:
23688307
22.

Role of Cdc48/p97 as a SUMO-targeted segregase curbing Rad51-Rad52 interaction.

Bergink S, Ammon T, Kern M, Schermelleh L, Leonhardt H, Jentsch S.

Nat Cell Biol. 2013 May;15(5):526-32. doi: 10.1038/ncb2729. Epub 2013 Apr 28.

PMID:
23624404
23.

Suv4-20h2 mediates chromatin compaction and is important for cohesin recruitment to heterochromatin.

Hahn M, Dambacher S, Dulev S, Kuznetsova AY, Eck S, Wörz S, Sadic D, Schulte M, Mallm JP, Maiser A, Debs P, von Melchner H, Leonhardt H, Schermelleh L, Rohr K, Rippe K, Storchova Z, Schotta G.

Genes Dev. 2013 Apr 15;27(8):859-72. doi: 10.1101/gad.210377.112. Epub 2013 Apr 18.

24.

Dissection of cell cycle-dependent dynamics of Dnmt1 by FRAP and diffusion-coupled modeling.

Schneider K, Fuchs C, Dobay A, Rottach A, Qin W, Wolf P, Álvarez-Castro JM, Nalaskowski MM, Kremmer E, Schmid V, Leonhardt H, Schermelleh L.

Nucleic Acids Res. 2013 May;41(9):4860-76. doi: 10.1093/nar/gkt191. Epub 2013 Mar 27.

25.

3D-structured illumination microscopy provides novel insight into architecture of human centrosomes.

Sonnen KF, Schermelleh L, Leonhardt H, Nigg EA.

Biol Open. 2012 Oct 15;1(10):965-76. doi: 10.1242/bio.20122337. Epub 2012 Aug 8.

26.

Fluorescence in situ hybridization applications for super-resolution 3D structured illumination microscopy.

Markaki Y, Smeets D, Cremer M, Schermelleh L.

Methods Mol Biol. 2013;950:43-64. doi: 10.1007/978-1-62703-137-0_4.

PMID:
23086869
27.

Dynein light chain 1 and a spindle-associated adaptor promote dynein asymmetry and spindle orientation.

Dunsch AK, Hammond D, Lloyd J, Schermelleh L, Gruneberg U, Barr FA.

J Cell Biol. 2012 Sep 17;198(6):1039-54. doi: 10.1083/jcb.201202112. Epub 2012 Sep 10.

28.

Myb-binding protein 1a (Mybbp1a) regulates levels and processing of pre-ribosomal RNA.

Hochstatter J, Hölzel M, Rohrmoser M, Schermelleh L, Leonhardt H, Keough R, Gonda TJ, Imhof A, Eick D, Längst G, Németh A.

J Biol Chem. 2012 Jul 13;287(29):24365-77. doi: 10.1074/jbc.M111.303719. Epub 2012 May 29.

30.

Analysis of centriole elimination during C. elegans oogenesis.

Mikeladze-Dvali T, von Tobel L, Strnad P, Knott G, Leonhardt H, Schermelleh L, Gönczy P.

Development. 2012 May;139(9):1670-9. doi: 10.1242/dev.075440.

31.

H2A.Z.2.2 is an alternatively spliced histone H2A.Z variant that causes severe nucleosome destabilization.

Bönisch C, Schneider K, Pünzeler S, Wiedemann SM, Bielmeier C, Bocola M, Eberl HC, Kuegel W, Neumann J, Kremmer E, Leonhardt H, Mann M, Michaelis J, Schermelleh L, Hake SB.

Nucleic Acids Res. 2012 Jul;40(13):5951-64. Epub 2012 Mar 29.

32.

ATRX-mediated chromatin association of histone variant macroH2A1 regulates α-globin expression.

Ratnakumar K, Duarte LF, LeRoy G, Hasson D, Smeets D, Vardabasso C, Bönisch C, Zeng T, Xiang B, Zhang DY, Li H, Wang X, Hake SB, Schermelleh L, Garcia BA, Bernstein E.

Genes Dev. 2012 Mar 1;26(5):433-8. doi: 10.1101/gad.179416.111.

33.

Functional nuclear organization of transcription and DNA replication: a topographical marriage between chromatin domains and the interchromatin compartment.

Markaki Y, Gunkel M, Schermelleh L, Beichmanis S, Neumann J, Heidemann M, Leonhardt H, Eick D, Cremer C, Cremer T.

Cold Spring Harb Symp Quant Biol. 2010;75:475-92. doi: 10.1101/sqb.2010.75.042. Epub 2011 Apr 5.

PMID:
21467142
34.

Mechanisms and advancement of antifading agents for fluorescence microscopy and single-molecule spectroscopy.

Cordes T, Maiser A, Steinhauer C, Schermelleh L, Tinnefeld P.

Phys Chem Chem Phys. 2011 Apr 14;13(14):6699-709. doi: 10.1039/c0cp01919d. Epub 2011 Feb 11.

PMID:
21311807
35.

Cortical constriction during abscission involves helices of ESCRT-III-dependent filaments.

Guizetti J, Schermelleh L, Mäntler J, Maar S, Poser I, Leonhardt H, Müller-Reichert T, Gerlich DW.

Science. 2011 Mar 25;331(6024):1616-20. doi: 10.1126/science.1201847. Epub 2011 Feb 10.

36.

Identification and characterization of two novel primate-specific histone H3 variants, H3.X and H3.Y.

Wiedemann SM, Mildner SN, Bönisch C, Israel L, Maiser A, Matheisl S, Straub T, Merkl R, Leonhardt H, Kremmer E, Schermelleh L, Hake SB.

J Cell Biol. 2010 Sep 6;190(5):777-91. doi: 10.1083/jcb.201002043.

37.

A guide to super-resolution fluorescence microscopy.

Schermelleh L, Heintzmann R, Leonhardt H.

J Cell Biol. 2010 Jul 26;190(2):165-75. doi: 10.1083/jcb.201002018. Epub 2010 Jul 19. Review.

38.

Genomic integration of adenoviral gene transfer vectors following transduction of fertilized mouse oocytes.

Larochelle N, Stucka R, Rieger N, Schermelleh L, Schiedner G, Kochanek S, Wolf E, Lochmüller H.

Transgenic Res. 2011 Feb;20(1):123-35. doi: 10.1007/s11248-010-9401-x. Epub 2010 May 13.

PMID:
20464633
39.

Measurement of replication structures at the nanometer scale using super-resolution light microscopy.

Baddeley D, Chagin VO, Schermelleh L, Martin S, Pombo A, Carlton PM, Gahl A, Domaing P, Birk U, Leonhardt H, Cremer C, Cardoso MC.

Nucleic Acids Res. 2010 Jan;38(2):e8. doi: 10.1093/nar/gkp901. Epub 2009 Oct 28.

40.

Visualization and measurement of DNA methyltransferase activity in living cells.

Schermelleh L, Spada F, Leonhardt H.

Curr Protoc Cell Biol. 2008 Jun;Chapter 22:Unit 22.12. doi: 10.1002/0471143030.cb2212s39.

PMID:
18551419
41.

Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy.

Schermelleh L, Carlton PM, Haase S, Shao L, Winoto L, Kner P, Burke B, Cardoso MC, Agard DA, Gustafsson MG, Leonhardt H, Sedat JW.

Science. 2008 Jun 6;320(5881):1332-6. doi: 10.1126/science.1156947.

42.

Synthesis of DNA dumbbell based inhibitors for the human DNA methyltransferase Dnmt1.

Kuch D, Schermelleh L, Manetto S, Leonhardt H, Carell T.

Angew Chem Int Ed Engl. 2008;47(8):1515-8. doi: 10.1002/anie.200702055. No abstract available.

PMID:
18203234
43.

Dynamics of Dnmt1 interaction with the replication machinery and its role in postreplicative maintenance of DNA methylation.

Schermelleh L, Haemmer A, Spada F, Rösing N, Meilinger D, Rothbauer U, Cardoso MC, Leonhardt H.

Nucleic Acids Res. 2007;35(13):4301-12. Epub 2007 Jun 18.

44.

DNMT1 but not its interaction with the replication machinery is required for maintenance of DNA methylation in human cells.

Spada F, Haemmer A, Kuch D, Rothbauer U, Schermelleh L, Kremmer E, Carell T, Längst G, Leonhardt H.

J Cell Biol. 2007 Feb 26;176(5):565-71. Epub 2007 Feb 20.

45.

Chromatin domains and the interchromatin compartment form structurally defined and functionally interacting nuclear networks.

Albiez H, Cremer M, Tiberi C, Vecchio L, Schermelleh L, Dittrich S, Küpper K, Joffe B, Thormeyer T, von Hase J, Yang S, Rohr K, Leonhardt H, Solovei I, Cremer C, Fakan S, Cremer T.

Chromosome Res. 2006;14(7):707-33. Epub 2006 Nov 22.

PMID:
17115328
46.

Targeting and tracing antigens in live cells with fluorescent nanobodies.

Rothbauer U, Zolghadr K, Tillib S, Nowak D, Schermelleh L, Gahl A, Backmann N, Conrath K, Muyldermans S, Cardoso MC, Leonhardt H.

Nat Methods. 2006 Nov;3(11):887-9.

PMID:
17060912
47.

Regulation of DNA methyltransferase 1.

Spada F, Rothbauer U, Zolghadr K, Schermelleh L, Leonhardt H.

Adv Enzyme Regul. 2006;46:224-34. Epub 2006 Jul 20. Review. No abstract available.

PMID:
16859735
48.

Trapped in action: direct visualization of DNA methyltransferase activity in living cells.

Schermelleh L, Spada F, Easwaran HP, Zolghadr K, Margot JB, Cardoso MC, Leonhardt H.

Nat Methods. 2005 Oct;2(10):751-6.

PMID:
16179921
49.

Restricted mobility of Dnmt1 in preimplantation embryos: implications for epigenetic reprogramming.

Grohmann M, Spada F, Schermelleh L, Alenina N, Bader M, Cardoso MC, Leonhardt H.

BMC Dev Biol. 2005 Aug 24;5:18.

50.

Recruitment of DNA methyltransferase I to DNA repair sites.

Mortusewicz O, Schermelleh L, Walter J, Cardoso MC, Leonhardt H.

Proc Natl Acad Sci U S A. 2005 Jun 21;102(25):8905-9. Epub 2005 Jun 13.

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